Candidatus Tenderia electrophaga
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3329 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S2TEN0|A0A0S2TEN0_9GAMM Response regulatory domain-containing protein OS=Candidatus Tenderia electrophaga OX=1748243 GN=Tel_10975 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 9.15 NTAAVICFLTTVIPGLSMAQLGYY25 pKa = 11.05 GPVEE29 pKa = 4.2 GDD31 pKa = 3.51 RR32 pKa = 11.84 EE33 pKa = 4.12 LSVAGTGSSEE43 pKa = 4.11 SDD45 pKa = 3.18 FDD47 pKa = 3.9 NGSFGITGDD56 pKa = 3.42 MGWYY60 pKa = 10.15 FRR62 pKa = 11.84 DD63 pKa = 3.34 NMVAGVRR70 pKa = 11.84 QSINYY75 pKa = 9.82 ASIEE79 pKa = 4.13 GASLNDD85 pKa = 3.87 DD86 pKa = 4.15 FWNGSTRR93 pKa = 11.84 GYY95 pKa = 11.01 LNYY98 pKa = 10.19 QFGSSQWRR106 pKa = 11.84 PFVGGSLGMIYY117 pKa = 10.68 GDD119 pKa = 3.56 GVNNSGFAGVEE130 pKa = 3.98 LGLKK134 pKa = 10.18 YY135 pKa = 10.63 YY136 pKa = 10.81 VLAKK140 pKa = 9.9 TYY142 pKa = 10.28 ILARR146 pKa = 11.84 LDD148 pKa = 3.83 YY149 pKa = 10.85 QWFFDD154 pKa = 5.14 DD155 pKa = 6.07 SDD157 pKa = 6.26 DD158 pKa = 5.27 IDD160 pKa = 3.84 DD161 pKa = 4.58 TFDD164 pKa = 4.1 DD165 pKa = 5.0 GAWQNTVGIGFNFF178 pKa = 3.6
Molecular weight: 19.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.834
IPC_protein 3.834
Toseland 3.592
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.503
Solomon 3.821
Lehninger 3.783
Nozaki 3.961
DTASelect 4.24
Thurlkill 3.668
EMBOSS 3.821
Sillero 3.948
Patrickios 0.998
IPC_peptide 3.821
IPC2_peptide 3.923
IPC2.peptide.svr19 3.845
Protein with the highest isoelectric point:
>tr|A0A0S2TGJ6|A0A0S2TGJ6_9GAMM T2SSC domain-containing protein OS=Candidatus Tenderia electrophaga OX=1748243 GN=Tel_14740 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.04 RR14 pKa = 11.84 AHH16 pKa = 6.12 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 8.88 ILKK32 pKa = 9.29 NRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.8 GRR39 pKa = 11.84 KK40 pKa = 8.69 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3329
0
3329
1089595
44
2846
327.3
36.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.564 ± 0.056
0.933 ± 0.015
5.947 ± 0.033
6.187 ± 0.043
3.605 ± 0.027
7.679 ± 0.038
2.435 ± 0.024
5.304 ± 0.033
3.683 ± 0.044
10.827 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.521 ± 0.021
3.13 ± 0.03
4.576 ± 0.027
4.443 ± 0.03
6.623 ± 0.041
5.408 ± 0.032
4.822 ± 0.029
7.171 ± 0.039
1.347 ± 0.021
2.795 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here