Desemzia incerta

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Carnobacteriaceae; Desemzia

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2143 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I5WW93|A0A1I5WW93_9LACT Uncharacterized protein OS=Desemzia incerta OX=82801 GN=SAMN04488506_1142 PE=4 SV=1
MM1 pKa = 7.46NKK3 pKa = 10.22LKK5 pKa = 9.95TKK7 pKa = 10.52KK8 pKa = 10.44SFLLSLVSAGVLAGCALPGLGSGSGEE34 pKa = 3.98EE35 pKa = 4.92GITVAGVTSTEE46 pKa = 4.01GLIMSYY52 pKa = 10.01VVEE55 pKa = 4.62GMIEE59 pKa = 4.15HH60 pKa = 6.44YY61 pKa = 10.07MDD63 pKa = 5.25DD64 pKa = 5.22LDD66 pKa = 4.02VQVINNLGSSTVSHH80 pKa = 6.05QALLNGDD87 pKa = 3.9ANIAGVKK94 pKa = 8.1YY95 pKa = 9.17TGTSLTGEE103 pKa = 4.28LGEE106 pKa = 5.87DD107 pKa = 4.84PITDD111 pKa = 3.54PEE113 pKa = 4.18KK114 pKa = 10.03ALEE117 pKa = 4.23VVVEE121 pKa = 4.32GFDD124 pKa = 5.04KK125 pKa = 11.2EE126 pKa = 5.31FDD128 pKa = 4.04LKK130 pKa = 10.51WFPSYY135 pKa = 11.12GFANSYY141 pKa = 11.21AFMVTKK147 pKa = 9.23EE148 pKa = 3.91TAEE151 pKa = 4.02EE152 pKa = 4.14YY153 pKa = 10.69GLEE156 pKa = 4.46KK157 pKa = 10.41ISDD160 pKa = 4.15LEE162 pKa = 4.33PYY164 pKa = 9.75ALEE167 pKa = 4.13MNAGVDD173 pKa = 3.47NSWIEE178 pKa = 4.06RR179 pKa = 11.84EE180 pKa = 3.92GDD182 pKa = 3.55GYY184 pKa = 11.29DD185 pKa = 4.46AFLDD189 pKa = 3.92TYY191 pKa = 11.12GFDD194 pKa = 3.99FNRR197 pKa = 11.84VYY199 pKa = 10.91PMQIGLVYY207 pKa = 10.6DD208 pKa = 4.24ALQAGSMDD216 pKa = 4.14IILGYY221 pKa = 8.9STDD224 pKa = 3.62GRR226 pKa = 11.84IASYY230 pKa = 11.21DD231 pKa = 3.78LVVLEE236 pKa = 6.01DD237 pKa = 4.68DD238 pKa = 4.35LQLFPPYY245 pKa = 9.88DD246 pKa = 4.19ASPLATNEE254 pKa = 3.66ILEE257 pKa = 4.63TYY259 pKa = 10.09PEE261 pKa = 4.36LEE263 pKa = 5.11GILLRR268 pKa = 11.84MEE270 pKa = 4.15GMISEE275 pKa = 4.35EE276 pKa = 3.96QMQEE280 pKa = 3.65MNYY283 pKa = 10.31EE284 pKa = 3.82ADD286 pKa = 3.84NNLKK290 pKa = 9.89EE291 pKa = 4.43PKK293 pKa = 10.14VVADD297 pKa = 6.25DD298 pKa = 3.96FLEE301 pKa = 4.58DD302 pKa = 3.19NNYY305 pKa = 10.02FEE307 pKa = 6.25DD308 pKa = 4.76GSSNTEE314 pKa = 3.64GGEE317 pKa = 3.95

Molecular weight: 34.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I5XFU5|A0A1I5XFU5_9LACT Inosine-5'-monophosphate dehydrogenase OS=Desemzia incerta OX=82801 GN=guaB PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.7QPKK8 pKa = 8.83KK9 pKa = 9.05RR10 pKa = 11.84KK11 pKa = 7.43RR12 pKa = 11.84QKK14 pKa = 8.87VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.83NGRR28 pKa = 11.84NVLQSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.58GRR39 pKa = 11.84KK40 pKa = 8.76VLSAA44 pKa = 4.05

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2143

0

2143

661021

27

1518

308.5

34.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.104 ± 0.054

0.493 ± 0.012

5.4 ± 0.041

8.046 ± 0.065

4.39 ± 0.041

6.696 ± 0.056

1.841 ± 0.02

7.622 ± 0.05

6.593 ± 0.049

9.636 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.771 ± 0.021

4.551 ± 0.032

3.463 ± 0.024

3.912 ± 0.039

3.728 ± 0.036

6.17 ± 0.037

5.891 ± 0.033

7.156 ± 0.04

0.881 ± 0.018

3.656 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski