Tenacibaculum adriaticum
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2925 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5S5DUB3|A0A5S5DUB3_9FLAO DNA repair protein RecN OS=Tenacibaculum adriaticum OX=413713 GN=C7447_102697 PE=3 SV=1
MM1 pKa = 7.27 NLIKK5 pKa = 10.45 KK6 pKa = 10.4 SSIQLIIIILFISITACNGDD26 pKa = 3.97 DD27 pKa = 5.44 DD28 pKa = 6.4 DD29 pKa = 7.13 DD30 pKa = 4.93 EE31 pKa = 5.01 FGNWVEE37 pKa = 4.53 SSIFDD42 pKa = 3.62 GDD44 pKa = 3.39 SRR46 pKa = 11.84 ANAVSFTIGTNGYY59 pKa = 9.86 LVTGYY64 pKa = 10.78 DD65 pKa = 3.58 GDD67 pKa = 5.54 DD68 pKa = 3.69 YY69 pKa = 11.84 LSDD72 pKa = 2.86 TWEE75 pKa = 4.16 YY76 pKa = 11.42 NSEE79 pKa = 3.74 DD80 pKa = 4.42 DD81 pKa = 4.01 YY82 pKa = 10.15 WTKK85 pKa = 10.83 KK86 pKa = 10.76 ADD88 pKa = 3.74 FPGIARR94 pKa = 11.84 SGAVGFSIDD103 pKa = 3.16 GKK105 pKa = 10.63 GYY107 pKa = 10.61 LGTGYY112 pKa = 10.67 DD113 pKa = 5.55 GEE115 pKa = 5.73 DD116 pKa = 3.59 EE117 pKa = 6.12 LNDD120 pKa = 3.45 FWEE123 pKa = 4.51 YY124 pKa = 11.64 NPDD127 pKa = 3.47 TDD129 pKa = 4.74 SWTQKK134 pKa = 11.08 ADD136 pKa = 3.5 FGGTARR142 pKa = 11.84 YY143 pKa = 8.63 GAIGFSLNNDD153 pKa = 3.52 GYY155 pKa = 10.59 IGTGYY160 pKa = 10.59 DD161 pKa = 3.23 GSEE164 pKa = 3.78 QKK166 pKa = 11.07 DD167 pKa = 3.41 FWKK170 pKa = 11.17 YY171 pKa = 8.7 NTTTDD176 pKa = 2.32 TWEE179 pKa = 3.94 QVVGFGGQKK188 pKa = 10.22 RR189 pKa = 11.84 KK190 pKa = 9.78 DD191 pKa = 3.35 ASVFTIDD198 pKa = 3.22 NIAYY202 pKa = 9.46 IGLGIHH208 pKa = 6.09 NGSYY212 pKa = 11.0 EE213 pKa = 3.98 EE214 pKa = 4.84 DD215 pKa = 4.02 FYY217 pKa = 12.0 SFDD220 pKa = 3.24 GSIWTRR226 pKa = 11.84 LSDD229 pKa = 5.63 LDD231 pKa = 6.01 DD232 pKa = 6.65 DD233 pKa = 6.52 DD234 pKa = 7.75 DD235 pKa = 7.54 DD236 pKa = 7.25 DD237 pKa = 6.78 DD238 pKa = 6.67 DD239 pKa = 5.68 YY240 pKa = 11.91 EE241 pKa = 5.08 ILLSSGVGFSINGKK255 pKa = 10.11 GYY257 pKa = 8.19 ITTGVSGSITTEE269 pKa = 3.28 SWEE272 pKa = 4.22 YY273 pKa = 10.49 TPSTDD278 pKa = 2.79 TWEE281 pKa = 4.08 EE282 pKa = 3.83 LPAFEE287 pKa = 5.74 GSARR291 pKa = 11.84 QNASAFTFATSAYY304 pKa = 9.65 VLMGRR309 pKa = 11.84 SGSYY313 pKa = 10.52 YY314 pKa = 10.31 FDD316 pKa = 4.55 DD317 pKa = 3.26 VWEE320 pKa = 4.43 FRR322 pKa = 11.84 PDD324 pKa = 3.3 EE325 pKa = 4.77 LEE327 pKa = 4.5 NEE329 pKa = 4.27 DD330 pKa = 4.74 DD331 pKa = 3.7
Molecular weight: 36.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.617
IPC_protein 3.656
Toseland 3.414
ProMoST 3.808
Dawson 3.656
Bjellqvist 3.821
Wikipedia 3.605
Rodwell 3.478
Grimsley 3.325
Solomon 3.656
Lehninger 3.605
Nozaki 3.77
DTASelect 4.037
Thurlkill 3.478
EMBOSS 3.617
Sillero 3.783
Patrickios 1.011
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.728
Protein with the highest isoelectric point:
>tr|A0A5S5DTX7|A0A5S5DTX7_9FLAO Phytoene/squalene synthetase OS=Tenacibaculum adriaticum OX=413713 GN=C7447_102683 PE=4 SV=1
MM1 pKa = 8.03 PKK3 pKa = 8.97 RR4 pKa = 11.84 TYY6 pKa = 10.33 QPSKK10 pKa = 9.01 RR11 pKa = 11.84 KK12 pKa = 9.47 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.27 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.04 GRR40 pKa = 11.84 KK41 pKa = 8.64 SLTVSSAPRR50 pKa = 11.84 PKK52 pKa = 10.38 KK53 pKa = 10.5
Molecular weight: 6.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.359
IPC2_protein 10.921
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.281
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.003
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.043
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2925
0
2925
971420
30
2624
332.1
37.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.896 ± 0.047
0.696 ± 0.014
5.402 ± 0.042
6.744 ± 0.044
5.376 ± 0.039
6.203 ± 0.046
1.699 ± 0.022
8.385 ± 0.049
8.613 ± 0.056
9.228 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.074 ± 0.021
6.54 ± 0.052
3.17 ± 0.023
3.311 ± 0.024
3.181 ± 0.031
6.499 ± 0.038
5.821 ± 0.046
6.182 ± 0.039
0.979 ± 0.016
3.999 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here