Caldivirga maquilingensis (strain ATCC 700844 / DSM 13496 / JCM 10307 / IC-167)
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1962 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A8ME74|A8ME74_CALMQ Transcriptional regulator TrmB OS=Caldivirga maquilingensis (strain ATCC 700844 / DSM 13496 / JCM 10307 / IC-167) OX=397948 GN=Cmaq_1253 PE=4 SV=1
MM1 pKa = 7.78 GIMLTRR7 pKa = 11.84 LYY9 pKa = 10.84 ARR11 pKa = 11.84 GGITVALILAVIAIITVIMVMLIHH35 pKa = 6.91 KK36 pKa = 8.88 GSQPAYY42 pKa = 10.46 SPVTQPITLPSGVSVLVIGPPSFVEE67 pKa = 3.9 DD68 pKa = 3.34 VGRR71 pKa = 11.84 IIPVAEE77 pKa = 4.2 SFSVDD82 pKa = 3.8 SIQEE86 pKa = 4.06 SPAGSVVIVDD96 pKa = 3.22 WDD98 pKa = 3.85 YY99 pKa = 11.84 LVNQVGVKK107 pKa = 10.27 GAVSDD112 pKa = 4.15 LSILFKK118 pKa = 11.15 QNDD121 pKa = 3.39 FMIVYY126 pKa = 10.59 SNDD129 pKa = 3.1 SFLAAYY135 pKa = 9.12 EE136 pKa = 4.22 LGRR139 pKa = 11.84 AWAMANGVNFTAIPAGVVGSFVAAFGDD166 pKa = 4.48 SKK168 pKa = 11.24 HH169 pKa = 5.6 LTYY172 pKa = 10.81 TSFNKK177 pKa = 8.63 PTEE180 pKa = 3.98 LLGAIAKK187 pKa = 9.89 YY188 pKa = 10.84 LEE190 pKa = 5.24 LKK192 pKa = 10.56 QSWQGTGQGYY202 pKa = 9.45 SFSPTRR208 pKa = 11.84 VLLQSNQQPTPDD220 pKa = 3.31 PCYY223 pKa = 10.39 QYY225 pKa = 10.31 GTQEE229 pKa = 4.02 GSSNGILFDD238 pKa = 4.89 YY239 pKa = 10.26 LPQYY243 pKa = 11.09 NGVTGEE249 pKa = 4.6 AYY251 pKa = 10.64 SDD253 pKa = 3.89 GNGTFYY259 pKa = 11.33 YY260 pKa = 8.14 DD261 pKa = 3.27 TCIFIYY267 pKa = 10.1 IYY269 pKa = 10.56 GIYY272 pKa = 9.42 YY273 pKa = 10.27 AADD276 pKa = 3.72 GTPSMDD282 pKa = 3.05 VVPAVWIAYY291 pKa = 8.44 VPSSTMVNNGGYY303 pKa = 9.25 INYY306 pKa = 9.44 YY307 pKa = 9.91 VGTIDD312 pKa = 4.39 HH313 pKa = 6.6 EE314 pKa = 4.67 KK315 pKa = 10.61 GYY317 pKa = 10.29 EE318 pKa = 3.9 AYY320 pKa = 10.04 EE321 pKa = 3.83 EE322 pKa = 5.45 GITNSHH328 pKa = 6.59 IDD330 pKa = 3.39 FAGGYY335 pKa = 9.19 SPGSASGEE343 pKa = 4.17 SPLTVNINYY352 pKa = 8.1 EE353 pKa = 4.17 NTGGATVNNTWTFTFSGYY371 pKa = 9.87 PNAGQTYY378 pKa = 8.67 SVAYY382 pKa = 8.94 MDD384 pKa = 4.89 DD385 pKa = 4.08 SAWMLTQGTNNEE397 pKa = 3.83 NTALLGNEE405 pKa = 3.65 IGVNLVTSAQYY416 pKa = 9.26 YY417 pKa = 8.78 VCFDD421 pKa = 3.4 ILNYY425 pKa = 10.72 EE426 pKa = 4.01 YY427 pKa = 10.16 MYY429 pKa = 11.71 VNFTWLLIYY438 pKa = 10.91 NPGQKK443 pKa = 8.21 PTYY446 pKa = 10.02 NATTHH451 pKa = 6.51 PTTSDD456 pKa = 3.28 PFITGITSYY465 pKa = 10.47 SQYY468 pKa = 10.88 IPIPCIGG475 pKa = 3.38
Molecular weight: 51.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.15
IPC2_protein 4.164
IPC_protein 4.139
Toseland 3.948
ProMoST 4.279
Dawson 4.113
Bjellqvist 4.266
Wikipedia 4.012
Rodwell 3.973
Grimsley 3.859
Solomon 4.101
Lehninger 4.062
Nozaki 4.215
DTASelect 4.418
Thurlkill 3.973
EMBOSS 4.024
Sillero 4.253
Patrickios 0.006
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.169
Protein with the highest isoelectric point:
>tr|A8MB39|A8MB39_CALMQ UspA domain protein OS=Caldivirga maquilingensis (strain ATCC 700844 / DSM 13496 / JCM 10307 / IC-167) OX=397948 GN=Cmaq_1851 PE=4 SV=1
MM1 pKa = 7.4 GKK3 pKa = 9.47 IKK5 pKa = 10.3 PSRR8 pKa = 11.84 TRR10 pKa = 11.84 EE11 pKa = 3.78 FGKK14 pKa = 10.82 GSLRR18 pKa = 11.84 CVRR21 pKa = 11.84 CGTHH25 pKa = 5.25 EE26 pKa = 3.99 AVIRR30 pKa = 11.84 RR31 pKa = 11.84 YY32 pKa = 10.83 GLMLCRR38 pKa = 11.84 RR39 pKa = 11.84 CFRR42 pKa = 11.84 EE43 pKa = 3.83 VAPQLGFKK51 pKa = 10.13 KK52 pKa = 10.72 YY53 pKa = 9.77 YY54 pKa = 10.09
Molecular weight: 6.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.736
IPC_protein 10.467
Toseland 10.599
ProMoST 10.321
Dawson 10.73
Bjellqvist 10.452
Wikipedia 10.921
Rodwell 10.994
Grimsley 10.774
Solomon 10.818
Lehninger 10.789
Nozaki 10.628
DTASelect 10.423
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.657
Patrickios 10.76
IPC_peptide 10.818
IPC2_peptide 9.736
IPC2.peptide.svr19 8.391
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1962
0
1962
600938
30
2938
306.3
34.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.162 ± 0.05
0.652 ± 0.019
4.499 ± 0.044
5.864 ± 0.059
3.29 ± 0.034
7.56 ± 0.053
1.456 ± 0.022
8.073 ± 0.05
4.965 ± 0.049
10.676 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.65 ± 0.024
4.638 ± 0.046
4.527 ± 0.039
1.871 ± 0.03
5.664 ± 0.05
6.685 ± 0.049
5.072 ± 0.062
8.981 ± 0.052
1.32 ± 0.025
4.395 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here