Raphidiopsis brookii D9

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Aphanizomenonaceae; Raphidiopsis; Raphidiopsis brookii

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3007 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4TMS5|D4TMS5_9CYAN Heat shock protein DnaJ-like protein OS=Raphidiopsis brookii D9 OX=533247 GN=CRD_00239 PE=4 SV=1
MM1 pKa = 7.68NDD3 pKa = 2.62GHH5 pKa = 6.56YY6 pKa = 10.77LIGLYY11 pKa = 9.92QSQLSISATTPAWTDD26 pKa = 2.63IDD28 pKa = 4.48ILDD31 pKa = 4.44NLDD34 pKa = 3.64NLIDD38 pKa = 3.96PSVFFTTDD46 pKa = 2.68LGKK49 pKa = 10.96YY50 pKa = 8.6GG51 pKa = 3.58

Molecular weight: 5.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4TV06|D4TV06_9CYAN Peptidoglycan glycosyltransferase RodA OS=Raphidiopsis brookii D9 OX=533247 GN=rodA PE=3 SV=1
MM1 pKa = 7.08QRR3 pKa = 11.84TLGGTCRR10 pKa = 11.84KK11 pKa = 9.61RR12 pKa = 11.84KK13 pKa = 7.61RR14 pKa = 11.84TSGFRR19 pKa = 11.84ARR21 pKa = 11.84MQTPTGRR28 pKa = 11.84NVIRR32 pKa = 11.84TRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.96KK37 pKa = 9.82GRR39 pKa = 11.84HH40 pKa = 5.0RR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3007

0

3007

904229

29

2249

300.7

33.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.036 ± 0.049

1.017 ± 0.015

4.762 ± 0.029

6.161 ± 0.046

3.899 ± 0.03

6.905 ± 0.04

1.99 ± 0.023

7.362 ± 0.035

5.162 ± 0.037

11.042 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.997 ± 0.02

4.579 ± 0.032

4.688 ± 0.032

5.179 ± 0.035

4.951 ± 0.031

6.461 ± 0.032

5.586 ± 0.029

6.644 ± 0.031

1.423 ± 0.025

3.157 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski