Lawsonella clevelandensis
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1376 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M5KZG0|A0A0M5KZG0_9ACTN Glycosyl transferase family 1 OS=Lawsonella clevelandensis OX=1528099 GN=AL705_01750 PE=4 SV=1
MM1 pKa = 7.14 ATDD4 pKa = 3.6 YY5 pKa = 10.35 DD6 pKa = 3.78 APRR9 pKa = 11.84 NNDD12 pKa = 3.03 ADD14 pKa = 4.0 GLEE17 pKa = 4.28 SSSLEE22 pKa = 3.76 EE23 pKa = 3.92 LQAGRR28 pKa = 11.84 GEE30 pKa = 4.34 SQAGSAEE37 pKa = 3.72 IDD39 pKa = 3.51 EE40 pKa = 4.61 VDD42 pKa = 3.29 TAEE45 pKa = 4.52 SYY47 pKa = 10.11 EE48 pKa = 4.27 LPGADD53 pKa = 5.14 LSDD56 pKa = 3.91 EE57 pKa = 4.19 EE58 pKa = 4.39 LTVRR62 pKa = 11.84 VVPKK66 pKa = 10.61 QKK68 pKa = 11.02 DD69 pKa = 3.33 EE70 pKa = 4.36 FTCGVCFLVQHH81 pKa = 6.9 ISRR84 pKa = 11.84 LAYY87 pKa = 9.27 IDD89 pKa = 3.96 EE90 pKa = 5.53 DD91 pKa = 3.8 GTMVCQDD98 pKa = 3.52 CAGG101 pKa = 3.34
Molecular weight: 10.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.77
IPC_protein 3.732
Toseland 3.528
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.643
Rodwell 3.567
Grimsley 3.439
Solomon 3.694
Lehninger 3.656
Nozaki 3.834
DTASelect 4.024
Thurlkill 3.579
EMBOSS 3.656
Sillero 3.846
Patrickios 1.85
IPC_peptide 3.694
IPC2_peptide 3.834
IPC2.peptide.svr19 3.775
Protein with the highest isoelectric point:
>tr|A0A0M3TB96|A0A0M3TB96_9ACTN Uncharacterized protein OS=Lawsonella clevelandensis OX=1528099 GN=AL705_00810 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 VHH19 pKa = 6.02 GFRR22 pKa = 11.84 ARR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 TLISRR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 AKK40 pKa = 10.52 GRR42 pKa = 11.84 ASLSAA47 pKa = 3.83
Molecular weight: 5.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.705
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.427
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.265
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1376
0
1376
460035
30
3412
334.3
36.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.071 ± 0.086
1.0 ± 0.021
6.008 ± 0.056
5.886 ± 0.074
3.084 ± 0.038
8.1 ± 0.051
2.423 ± 0.034
5.019 ± 0.052
3.376 ± 0.057
9.514 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.327 ± 0.027
2.954 ± 0.04
5.282 ± 0.052
3.527 ± 0.036
6.158 ± 0.061
5.734 ± 0.044
6.42 ± 0.052
8.184 ± 0.055
1.411 ± 0.031
2.523 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here