Pseudorhodoplanes sinuspersici

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiales incertae sedis; Pseudorhodoplanes

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5631 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1W6ZWH1|A0A1W6ZWH1_9RHIZ Protein-export membrane protein SecG OS=Pseudorhodoplanes sinuspersici OX=1235591 GN=CAK95_23085 PE=3 SV=1
MM1 pKa = 6.96QNARR5 pKa = 11.84FEE7 pKa = 4.83SVNTAATLRR16 pKa = 11.84TSSTIVQPSAATLEE30 pKa = 3.84NGILYY35 pKa = 7.6GTNRR39 pKa = 11.84DD40 pKa = 3.55DD41 pKa = 5.0VLFTTPQYY49 pKa = 10.71PHH51 pKa = 7.18LAGLTGADD59 pKa = 3.59RR60 pKa = 11.84LTGDD64 pKa = 3.27EE65 pKa = 4.49RR66 pKa = 11.84DD67 pKa = 3.26NRR69 pKa = 11.84LYY71 pKa = 11.09GGAHH75 pKa = 6.45NDD77 pKa = 3.34QLFGQDD83 pKa = 4.18GNDD86 pKa = 3.24ILDD89 pKa = 4.17GGTEE93 pKa = 3.99ADD95 pKa = 4.12RR96 pKa = 11.84LEE98 pKa = 4.65GGAGNDD104 pKa = 3.02IYY106 pKa = 11.52YY107 pKa = 10.25VDD109 pKa = 4.5HH110 pKa = 6.57FQDD113 pKa = 3.91VIIEE117 pKa = 4.2EE118 pKa = 4.28ADD120 pKa = 3.11GGYY123 pKa = 10.65DD124 pKa = 4.4IMFTNPDD131 pKa = 3.67GIYY134 pKa = 10.59GNSHH138 pKa = 6.0RR139 pKa = 11.84MALNVEE145 pKa = 4.1QMIMTGSGWQMGEE158 pKa = 4.8GNTQDD163 pKa = 3.16NFIIGSEE170 pKa = 4.17SEE172 pKa = 4.18NLILAYY178 pKa = 10.33GGDD181 pKa = 4.12DD182 pKa = 2.58IVYY185 pKa = 10.48GMGGTDD191 pKa = 4.41RR192 pKa = 11.84IYY194 pKa = 11.21GDD196 pKa = 5.0DD197 pKa = 4.13LNAPYY202 pKa = 10.51AGNDD206 pKa = 2.91ILYY209 pKa = 10.29GGEE212 pKa = 4.34GDD214 pKa = 3.97DD215 pKa = 4.47FLYY218 pKa = 11.0GGLGDD223 pKa = 4.23DD224 pKa = 3.64MLYY227 pKa = 10.82GGRR230 pKa = 11.84GVDD233 pKa = 3.49MLSGSGGNDD242 pKa = 2.55TGVFADD248 pKa = 4.41AEE250 pKa = 4.46SAVIIDD256 pKa = 3.71LTRR259 pKa = 11.84PYY261 pKa = 11.13NNGGSATGEE270 pKa = 3.85YY271 pKa = 10.45LFSIEE276 pKa = 4.36NLIGSAFNDD285 pKa = 4.04TLSGGQSRR293 pKa = 11.84NMLEE297 pKa = 4.43GGDD300 pKa = 3.83GNDD303 pKa = 2.93RR304 pKa = 11.84LFGRR308 pKa = 11.84EE309 pKa = 3.6GGDD312 pKa = 3.45TVRR315 pKa = 11.84GGAGSDD321 pKa = 4.13FIDD324 pKa = 4.03GGANRR329 pKa = 11.84DD330 pKa = 3.75VLTGGAGIGDD340 pKa = 4.27APDD343 pKa = 3.26TFYY346 pKa = 10.92FASAAEE352 pKa = 4.42AGDD355 pKa = 3.94TITDD359 pKa = 4.58FFSDD363 pKa = 4.66HH364 pKa = 6.59IALSAEE370 pKa = 4.07GFGLEE375 pKa = 4.41SVDD378 pKa = 3.92DD379 pKa = 4.04FVFARR384 pKa = 11.84TSDD387 pKa = 3.97PLTDD391 pKa = 4.32MPTMIYY397 pKa = 10.5DD398 pKa = 3.63SRR400 pKa = 11.84SGNLSWDD407 pKa = 3.42ADD409 pKa = 3.77GSGGEE414 pKa = 3.94AAVYY418 pKa = 9.09FATLTGAPLLTQNDD432 pKa = 3.77FLIII436 pKa = 3.93

Molecular weight:
45.93 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1W6ZRP6|A0A1W6ZRP6_9RHIZ Gamma-glutamyltransferase OS=Pseudorhodoplanes sinuspersici OX=1235591 GN=CAK95_10305 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.31VIAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight:
5.14 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5631

0

5631

1729092

31

2510

307.1

33.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.984 ± 0.039

0.842 ± 0.011

5.627 ± 0.028

5.282 ± 0.029

3.874 ± 0.023

8.214 ± 0.029

2.044 ± 0.016

5.678 ± 0.023

3.894 ± 0.027

9.746 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.565 ± 0.016

2.843 ± 0.02

5.305 ± 0.025

3.181 ± 0.017

6.922 ± 0.031

5.496 ± 0.021

5.395 ± 0.024

7.453 ± 0.023

1.321 ± 0.014

2.335 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski