Pseudorhodoplanes sinuspersici
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5631 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W6ZWH1|A0A1W6ZWH1_9RHIZ Protein-export membrane protein SecG OS=Pseudorhodoplanes sinuspersici OX=1235591 GN=CAK95_23085 PE=3 SV=1
MM1 pKa = 6.96 QNARR5 pKa = 11.84 FEE7 pKa = 4.83 SVNTAATLRR16 pKa = 11.84 TSSTIVQPSAATLEE30 pKa = 3.84 NGILYY35 pKa = 7.6 GTNRR39 pKa = 11.84 DD40 pKa = 3.55 DD41 pKa = 5.0 VLFTTPQYY49 pKa = 10.71 PHH51 pKa = 7.18 LAGLTGADD59 pKa = 3.59 RR60 pKa = 11.84 LTGDD64 pKa = 3.27 EE65 pKa = 4.49 RR66 pKa = 11.84 DD67 pKa = 3.26 NRR69 pKa = 11.84 LYY71 pKa = 11.09 GGAHH75 pKa = 6.45 NDD77 pKa = 3.34 QLFGQDD83 pKa = 4.18 GNDD86 pKa = 3.24 ILDD89 pKa = 4.17 GGTEE93 pKa = 3.99 ADD95 pKa = 4.12 RR96 pKa = 11.84 LEE98 pKa = 4.65 GGAGNDD104 pKa = 3.02 IYY106 pKa = 11.52 YY107 pKa = 10.25 VDD109 pKa = 4.5 HH110 pKa = 6.57 FQDD113 pKa = 3.91 VIIEE117 pKa = 4.2 EE118 pKa = 4.28 ADD120 pKa = 3.11 GGYY123 pKa = 10.65 DD124 pKa = 4.4 IMFTNPDD131 pKa = 3.67 GIYY134 pKa = 10.59 GNSHH138 pKa = 6.0 RR139 pKa = 11.84 MALNVEE145 pKa = 4.1 QMIMTGSGWQMGEE158 pKa = 4.8 GNTQDD163 pKa = 3.16 NFIIGSEE170 pKa = 4.17 SEE172 pKa = 4.18 NLILAYY178 pKa = 10.33 GGDD181 pKa = 4.12 DD182 pKa = 2.58 IVYY185 pKa = 10.48 GMGGTDD191 pKa = 4.41 RR192 pKa = 11.84 IYY194 pKa = 11.21 GDD196 pKa = 5.0 DD197 pKa = 4.13 LNAPYY202 pKa = 10.51 AGNDD206 pKa = 2.91 ILYY209 pKa = 10.29 GGEE212 pKa = 4.34 GDD214 pKa = 3.97 DD215 pKa = 4.47 FLYY218 pKa = 11.0 GGLGDD223 pKa = 4.23 DD224 pKa = 3.64 MLYY227 pKa = 10.82 GGRR230 pKa = 11.84 GVDD233 pKa = 3.49 MLSGSGGNDD242 pKa = 2.55 TGVFADD248 pKa = 4.41 AEE250 pKa = 4.46 SAVIIDD256 pKa = 3.71 LTRR259 pKa = 11.84 PYY261 pKa = 11.13 NNGGSATGEE270 pKa = 3.85 YY271 pKa = 10.45 LFSIEE276 pKa = 4.36 NLIGSAFNDD285 pKa = 4.04 TLSGGQSRR293 pKa = 11.84 NMLEE297 pKa = 4.43 GGDD300 pKa = 3.83 GNDD303 pKa = 2.93 RR304 pKa = 11.84 LFGRR308 pKa = 11.84 EE309 pKa = 3.6 GGDD312 pKa = 3.45 TVRR315 pKa = 11.84 GGAGSDD321 pKa = 4.13 FIDD324 pKa = 4.03 GGANRR329 pKa = 11.84 DD330 pKa = 3.75 VLTGGAGIGDD340 pKa = 4.27 APDD343 pKa = 3.26 TFYY346 pKa = 10.92 FASAAEE352 pKa = 4.42 AGDD355 pKa = 3.94 TITDD359 pKa = 4.58 FFSDD363 pKa = 4.66 HH364 pKa = 6.59 IALSAEE370 pKa = 4.07 GFGLEE375 pKa = 4.41 SVDD378 pKa = 3.92 DD379 pKa = 4.04 FVFARR384 pKa = 11.84 TSDD387 pKa = 3.97 PLTDD391 pKa = 4.32 MPTMIYY397 pKa = 10.5 DD398 pKa = 3.63 SRR400 pKa = 11.84 SGNLSWDD407 pKa = 3.42 ADD409 pKa = 3.77 GSGGEE414 pKa = 3.94 AAVYY418 pKa = 9.09 FATLTGAPLLTQNDD432 pKa = 3.77 FLIII436 pKa = 3.93
Molecular weight: 45.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.63
IPC_protein 3.681
Toseland 3.439
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.63
Rodwell 3.49
Grimsley 3.338
Solomon 3.668
Lehninger 3.63
Nozaki 3.783
DTASelect 4.075
Thurlkill 3.49
EMBOSS 3.643
Sillero 3.795
Patrickios 1.863
IPC_peptide 3.668
IPC2_peptide 3.77
IPC2.peptide.svr19 3.733
Protein with the highest isoelectric point:
>tr|A0A1W6ZRP6|A0A1W6ZRP6_9RHIZ Gamma-glutamyltransferase OS=Pseudorhodoplanes sinuspersici OX=1235591 GN=CAK95_10305 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.31 VIAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5631
0
5631
1729092
31
2510
307.1
33.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.984 ± 0.039
0.842 ± 0.011
5.627 ± 0.028
5.282 ± 0.029
3.874 ± 0.023
8.214 ± 0.029
2.044 ± 0.016
5.678 ± 0.023
3.894 ± 0.027
9.746 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.565 ± 0.016
2.843 ± 0.02
5.305 ± 0.025
3.181 ± 0.017
6.922 ± 0.031
5.496 ± 0.021
5.395 ± 0.024
7.453 ± 0.023
1.321 ± 0.014
2.335 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here