Amaricoccus sp. HAR-UPW-R2A-40
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4378 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2M9PGT8|A0A2M9PGT8_9RHOB NAD(P)H dehydrogenase (quinone) OS=Amaricoccus sp. HAR-UPW-R2A-40 OX=1985299 GN=CNY89_00685 PE=3 SV=1
MM1 pKa = 7.94 AYY3 pKa = 10.68 LDD5 pKa = 4.08 ANGVLLPTSRR15 pKa = 11.84 VASSWIGGGGALYY28 pKa = 10.77 GNSGDD33 pKa = 3.52 NGFYY37 pKa = 10.92 GSGDD41 pKa = 3.51 DD42 pKa = 4.03 TLTGGLGDD50 pKa = 3.99 DD51 pKa = 4.65 TYY53 pKa = 10.88 MVWVPSTTIVEE64 pKa = 4.2 AANGGVDD71 pKa = 3.58 TLDD74 pKa = 3.31 SRR76 pKa = 11.84 VWGEE80 pKa = 4.11 AILPEE85 pKa = 4.38 HH86 pKa = 7.02 VEE88 pKa = 3.97 NLLLNGPGTTAGTGNGLRR106 pKa = 11.84 NLIVAGNVGATLDD119 pKa = 4.01 GLAGDD124 pKa = 4.69 DD125 pKa = 4.02 VLVSGAGADD134 pKa = 3.33 IMRR137 pKa = 11.84 VQAGNGSDD145 pKa = 3.87 AIVGFVPGSDD155 pKa = 3.6 VIQLVGYY162 pKa = 9.38 GISTFDD168 pKa = 3.56 QLAQIAAQQGSDD180 pKa = 3.87 LVFTFSNDD188 pKa = 2.91 EE189 pKa = 4.1 KK190 pKa = 11.19 LVLRR194 pKa = 11.84 DD195 pKa = 3.7 VVLSDD200 pKa = 4.06 LDD202 pKa = 4.53 GYY204 pKa = 11.41 DD205 pKa = 3.77 FGLDD209 pKa = 3.35 QPLPPLPAGHH219 pKa = 6.28 QSLFGPGQAYY229 pKa = 10.28 SAFGWYY235 pKa = 9.48 VLNNVWNPGPLVYY248 pKa = 10.5 GVDD251 pKa = 3.89 YY252 pKa = 9.83 TVSSSYY258 pKa = 11.6 DD259 pKa = 3.48 PTDD262 pKa = 3.13 LTAGVTFHH270 pKa = 6.89 WAFPLTTNAFPTIIAYY286 pKa = 9.5 PEE288 pKa = 4.61 VIFGPAPMSGGHH300 pKa = 6.19 KK301 pKa = 9.44 VTDD304 pKa = 3.64 TAGVFPLQVSEE315 pKa = 4.69 IVDD318 pKa = 3.85 LTADD322 pKa = 3.55 YY323 pKa = 11.27 AVAIEE328 pKa = 4.35 GNTDD332 pKa = 3.36 GFNVAFDD339 pKa = 3.06 IWLTDD344 pKa = 3.44 VPNGGPSSVTNEE356 pKa = 3.58 VMVWVHH362 pKa = 6.17 KK363 pKa = 11.05 GGVTPYY369 pKa = 9.94 GQLAGTYY376 pKa = 10.44 DD377 pKa = 4.44 DD378 pKa = 5.2 GPVSAEE384 pKa = 4.02 IYY386 pKa = 10.78 VSDD389 pKa = 4.26 SGDD392 pKa = 2.9 WTYY395 pKa = 10.4 TAVVLDD401 pKa = 3.87 EE402 pKa = 4.85 DD403 pKa = 4.28 RR404 pKa = 11.84 LVGEE408 pKa = 4.43 ISVSGVLARR417 pKa = 11.84 LQALGIVSSSEE428 pKa = 3.65 YY429 pKa = 10.43 LASLEE434 pKa = 4.18 LGSEE438 pKa = 4.29 IVSGAGSLTIEE449 pKa = 4.85 DD450 pKa = 3.91 LTLNATLEE458 pKa = 4.13 DD459 pKa = 3.49 RR460 pKa = 11.84 TIEE463 pKa = 3.97 VTGAGTTTHH472 pKa = 7.57 LFPEE476 pKa = 4.99 DD477 pKa = 4.07 PPDD480 pKa = 3.6 LSGDD484 pKa = 3.65 DD485 pKa = 3.33 RR486 pKa = 11.84 VLYY489 pKa = 10.71 DD490 pKa = 3.57 PTQSLIEE497 pKa = 4.46 GGEE500 pKa = 4.21 GSDD503 pKa = 3.27 TLVLNVGATVRR514 pKa = 11.84 LDD516 pKa = 3.25 RR517 pKa = 11.84 FTTSQVDD524 pKa = 4.04 GPAYY528 pKa = 9.22 VTGFEE533 pKa = 4.43 NVDD536 pKa = 3.05 ASAANAGVTLYY547 pKa = 10.62 GSPYY551 pKa = 10.87 ANVLVGGAYY560 pKa = 9.28 TDD562 pKa = 3.78 TLSGGDD568 pKa = 3.51 GADD571 pKa = 3.41 VLRR574 pKa = 11.84 GGGGGDD580 pKa = 4.01 IIDD583 pKa = 4.56 GGAGADD589 pKa = 3.68 QIQGGDD595 pKa = 3.37 GNDD598 pKa = 3.7 RR599 pKa = 11.84 ITYY602 pKa = 9.63 DD603 pKa = 3.28 AADD606 pKa = 3.63 YY607 pKa = 11.18 SIDD610 pKa = 3.56 AGAGSDD616 pKa = 3.72 TLVLTVGATVRR627 pKa = 11.84 LDD629 pKa = 3.36 RR630 pKa = 11.84 FSTSQVDD637 pKa = 3.49 GGAYY641 pKa = 7.88 VTGFEE646 pKa = 4.7 KK647 pKa = 10.71 VDD649 pKa = 3.3 AAAASAAVNLTGSAYY664 pKa = 10.56 ANTLTGGSKK673 pKa = 9.99 RR674 pKa = 11.84 DD675 pKa = 3.68 VLTGGAGADD684 pKa = 2.96 QFVFKK689 pKa = 9.63 TAPKK693 pKa = 10.31 ASAADD698 pKa = 4.0 TITDD702 pKa = 4.04 FSVGEE707 pKa = 4.08 DD708 pKa = 4.06 RR709 pKa = 11.84 IHH711 pKa = 7.34 LDD713 pKa = 2.91 ASFFRR718 pKa = 11.84 GLPTGALASGALEE731 pKa = 4.39 FGTTAAASDD740 pKa = 3.76 DD741 pKa = 4.17 RR742 pKa = 11.84 ILYY745 pKa = 10.38 DD746 pKa = 3.44 SASGSLYY753 pKa = 10.37 FDD755 pKa = 4.19 RR756 pKa = 11.84 DD757 pKa = 3.33 GSADD761 pKa = 3.9 DD762 pKa = 3.82 YY763 pKa = 11.7 SAILFATIGPGKK775 pKa = 10.13 AVSAQDD781 pKa = 3.03 FWVIAA786 pKa = 3.95
Molecular weight: 80.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.681
IPC2_protein 3.643
IPC_protein 3.706
Toseland 3.452
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.363
Solomon 3.706
Lehninger 3.668
Nozaki 3.808
DTASelect 4.113
Thurlkill 3.516
EMBOSS 3.668
Sillero 3.821
Patrickios 1.481
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.759
Protein with the highest isoelectric point:
>tr|A0A2M9P8G0|A0A2M9P8G0_9RHOB Elongation factor Tu (Fragment) OS=Amaricoccus sp. HAR-UPW-R2A-40 OX=1985299 GN=tuf PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLVRR13 pKa = 11.84 ARR15 pKa = 11.84 RR16 pKa = 11.84 HH17 pKa = 4.37 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.19 AGRR29 pKa = 11.84 VILNRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AMGRR40 pKa = 11.84 KK41 pKa = 9.03 KK42 pKa = 10.63 LSAA45 pKa = 3.93
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4378
0
4378
800038
21
1506
182.7
19.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.635 ± 0.057
0.882 ± 0.015
5.728 ± 0.035
5.864 ± 0.039
3.841 ± 0.031
8.822 ± 0.046
2.001 ± 0.022
4.865 ± 0.033
2.824 ± 0.032
9.91 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.565 ± 0.02
2.398 ± 0.025
5.445 ± 0.033
2.942 ± 0.023
7.759 ± 0.06
5.08 ± 0.031
5.255 ± 0.033
7.392 ± 0.038
1.532 ± 0.021
2.259 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here