SAR202 cluster bacterium AD-812-D07_MRT_10900m

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Chloroflexi incertae sedis; SAR202 cluster

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 274 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N8YXV4|A0A5N8YXV4_9CHLR Uncharacterized protein OS=SAR202 cluster bacterium AD-812-D07_MRT_10900m OX=2587835 GN=FIL92_00715 PE=4 SV=1
MM1 pKa = 7.49ACAAGITNEE10 pKa = 4.3LLVPLTGSSEE20 pKa = 4.17MFSGAKK26 pKa = 9.99NKK28 pKa = 9.28WLVLVVVAALGAFVIACGTDD48 pKa = 3.39EE49 pKa = 4.81TPVTQNDD56 pKa = 4.41GVGSQPGQNGSGGGSIIADD75 pKa = 3.59SQALEE80 pKa = 4.08TALEE84 pKa = 4.12LEE86 pKa = 4.63GADD89 pKa = 3.77VEE91 pKa = 5.14FGVQSEE97 pKa = 4.29FAGFFGLRR105 pKa = 11.84PTEE108 pKa = 3.8LKK110 pKa = 11.27VNGEE114 pKa = 3.98SLLIYY119 pKa = 9.76EE120 pKa = 4.9FAPGTSAEE128 pKa = 4.19EE129 pKa = 4.03ASEE132 pKa = 4.33GVSPDD137 pKa = 3.65GITMVNPDD145 pKa = 3.43GSATSVFWAAPPHH158 pKa = 6.65FYY160 pKa = 11.14LFGNSILLYY169 pKa = 9.89NGNDD173 pKa = 3.49AEE175 pKa = 4.49IGALLGSVSVQFAGRR190 pKa = 11.84DD191 pKa = 3.7FEE193 pKa = 4.54EE194 pKa = 4.47VSNGSGSGSGDD205 pKa = 3.5LGDD208 pKa = 5.35PPPSDD213 pKa = 3.25PGFEE217 pKa = 4.25VVEE220 pKa = 3.92ALAPIEE226 pKa = 4.17SVEE229 pKa = 3.9ILTLEE234 pKa = 4.33SYY236 pKa = 10.54PEE238 pKa = 3.78QFIVQVTSSLPNGCASYY255 pKa = 10.88SHH257 pKa = 6.92NEE259 pKa = 3.84VTQDD263 pKa = 2.98GTDD266 pKa = 3.14IKK268 pKa = 10.65ISVYY272 pKa = 9.58NTVPAPGEE280 pKa = 4.26LIACTEE286 pKa = 4.36IYY288 pKa = 10.14RR289 pKa = 11.84LHH291 pKa = 6.83DD292 pKa = 3.55QNIGLGSDD300 pKa = 4.03FEE302 pKa = 5.33RR303 pKa = 11.84GTTYY307 pKa = 10.24TVLVNDD313 pKa = 4.42HH314 pKa = 6.99PGEE317 pKa = 4.25TFTTGSAPLPSGATPPAPEE336 pKa = 4.05VPVDD340 pKa = 3.89HH341 pKa = 7.03EE342 pKa = 4.96LVTAPIEE349 pKa = 4.09SLEE352 pKa = 4.35IIQGEE357 pKa = 4.3DD358 pKa = 2.92SRR360 pKa = 11.84GRR362 pKa = 11.84ATYY365 pKa = 9.36SARR368 pKa = 11.84VAWGLSDD375 pKa = 4.3GCKK378 pKa = 9.6QSYY381 pKa = 10.13NRR383 pKa = 11.84TISRR387 pKa = 11.84IDD389 pKa = 3.22EE390 pKa = 4.11TTFEE394 pKa = 4.07IKK396 pKa = 10.53AIVTSPTGDD405 pKa = 3.4VMCTLDD411 pKa = 3.93YY412 pKa = 10.42RR413 pKa = 11.84TDD415 pKa = 3.2SDD417 pKa = 4.48DD418 pKa = 4.16FYY420 pKa = 11.49LGAVGEE426 pKa = 4.49ALTACTVYY434 pKa = 10.71HH435 pKa = 6.39IVAGKK440 pKa = 10.23LRR442 pKa = 11.84VEE444 pKa = 4.39YY445 pKa = 10.4QAIAPNVRR453 pKa = 11.84CVDD456 pKa = 3.73PEE458 pKa = 4.17LTPSAGSGGGSIIADD473 pKa = 3.64SQALEE478 pKa = 4.36LSLEE482 pKa = 4.34SKK484 pKa = 10.69GADD487 pKa = 3.33VEE489 pKa = 4.51FGGASAFSKK498 pKa = 10.76LFGVAPSEE506 pKa = 4.3LKK508 pKa = 11.27VNGQAVQIYY517 pKa = 8.76QFAPGTSAEE526 pKa = 4.22EE527 pKa = 4.14ASEE530 pKa = 4.3SVSPGGTTIVNPDD543 pKa = 3.06GSVISVMWIAPPHH556 pKa = 6.41FYY558 pKa = 11.06LFGNAIILYY567 pKa = 9.33VGNDD571 pKa = 3.65PEE573 pKa = 5.41IGALLDD579 pKa = 3.71SVAGKK584 pKa = 9.58FAGSNFEE591 pKa = 4.4DD592 pKa = 3.85AGSGSGEE599 pKa = 3.62IDD601 pKa = 3.01NEE603 pKa = 3.92YY604 pKa = 10.37RR605 pKa = 11.84IQTAQIVRR613 pKa = 11.84VDD615 pKa = 3.28IASTRR620 pKa = 11.84SIPAQHH626 pKa = 6.72MISMTIALGGSCEE639 pKa = 4.06EE640 pKa = 4.41FAGLDD645 pKa = 3.17WRR647 pKa = 11.84VEE649 pKa = 3.91GRR651 pKa = 11.84EE652 pKa = 3.93VIIDD656 pKa = 3.62VTTKK660 pKa = 10.45VPTAPVPCTLAIIYY674 pKa = 8.53EE675 pKa = 4.22DD676 pKa = 3.37QSVNIGDD683 pKa = 3.77EE684 pKa = 4.39YY685 pKa = 10.06EE686 pKa = 4.17TGVEE690 pKa = 3.64YY691 pKa = 10.86DD692 pKa = 3.63VIVNGEE698 pKa = 4.01RR699 pKa = 11.84QGTFIGGG706 pKa = 3.31

Molecular weight: 73.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N8YYA7|A0A5N8YYA7_9CHLR DNA repair protein RadA OS=SAR202 cluster bacterium AD-812-D07_MRT_10900m OX=2587835 GN=radA PE=3 SV=1
MM1 pKa = 7.09EE2 pKa = 4.89WFRR5 pKa = 11.84RR6 pKa = 11.84WIVEE10 pKa = 4.41GYY12 pKa = 8.62SVRR15 pKa = 11.84QLALQSGHH23 pKa = 5.78SRR25 pKa = 11.84RR26 pKa = 11.84KK27 pKa = 9.29LRR29 pKa = 11.84RR30 pKa = 11.84LIDD33 pKa = 3.6SFLAQAPPTSALEE46 pKa = 4.13PQTARR51 pKa = 11.84YY52 pKa = 9.46LLFDD56 pKa = 3.35GTFLHH61 pKa = 6.86RR62 pKa = 11.84PHH64 pKa = 7.77SIVVLMNGQTHH75 pKa = 6.39RR76 pKa = 11.84LVRR79 pKa = 11.84GQFDD83 pKa = 3.36VRR85 pKa = 11.84EE86 pKa = 4.04NSGPQLRR93 pKa = 11.84AFFEE97 pKa = 4.23PMMDD101 pKa = 3.09EE102 pKa = 4.22GLRR105 pKa = 11.84PLSFTVDD112 pKa = 3.08GNRR115 pKa = 11.84QVIRR119 pKa = 11.84VLRR122 pKa = 11.84TLWPDD127 pKa = 3.27AVIQRR132 pKa = 11.84CLVHH136 pKa = 6.42IQRR139 pKa = 11.84QGLSWCRR146 pKa = 11.84ISPKK150 pKa = 9.85TPYY153 pKa = 10.46ARR155 pKa = 11.84QLRR158 pKa = 11.84DD159 pKa = 2.84IFLQVTKK166 pKa = 10.31IATPADD172 pKa = 3.61RR173 pKa = 11.84EE174 pKa = 4.27AFLNLVAIWEE184 pKa = 4.21EE185 pKa = 4.49RR186 pKa = 11.84YY187 pKa = 8.82GTEE190 pKa = 3.06IDD192 pKa = 3.21ARR194 pKa = 11.84KK195 pKa = 9.78EE196 pKa = 3.52IGRR199 pKa = 11.84VFSDD203 pKa = 3.27IKK205 pKa = 10.29RR206 pKa = 11.84ARR208 pKa = 11.84SMLMHH213 pKa = 7.46ALPDD217 pKa = 3.59MFHH220 pKa = 7.14YY221 pKa = 10.85LDD223 pKa = 4.92DD224 pKa = 3.81RR225 pKa = 11.84HH226 pKa = 7.25IPTTTNGLEE235 pKa = 4.65GYY237 pKa = 10.14FSRR240 pKa = 11.84LKK242 pKa = 9.89SHH244 pKa = 5.96YY245 pKa = 8.3RR246 pKa = 11.84QHH248 pKa = 7.21RR249 pKa = 11.84GLSPRR254 pKa = 11.84KK255 pKa = 9.15RR256 pKa = 11.84PNYY259 pKa = 7.99FAWYY263 pKa = 8.81FHH265 pKa = 6.28FTPKK269 pKa = 10.65

Molecular weight: 31.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

274

0

274

81315

52

1923

296.8

32.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.463 ± 0.162

0.767 ± 0.042

6.328 ± 0.125

6.384 ± 0.129

3.633 ± 0.084

8.654 ± 0.164

2.019 ± 0.073

5.695 ± 0.086

3.252 ± 0.126

9.066 ± 0.134

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.236 ± 0.071

3.264 ± 0.077

5.115 ± 0.11

2.972 ± 0.074

6.44 ± 0.184

6.921 ± 0.141

5.987 ± 0.161

7.926 ± 0.127

1.439 ± 0.061

2.439 ± 0.077
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski