Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) (Mucormycosis agent) (Rhizopus arrhizus var. delemar)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina; Mucoromycetes; Mucorales; Mucorineae; Rhizopodaceae; Rhizopus; Rhizopus delemar

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16971 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1BU05|I1BU05_RHIO9 Uncharacterized protein OS=Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) OX=246409 GN=RO3G_04390 PE=4 SV=1
MM1 pKa = 7.42YY2 pKa = 9.71YY3 pKa = 10.73SSITIISSILLATCSFVNAASTNSSYY29 pKa = 11.41PLTTINSQKK38 pKa = 10.41DD39 pKa = 3.29FCIFLPPQPGLEE51 pKa = 4.08VAVNEE56 pKa = 4.19NNGIPFCFNQDD67 pKa = 3.19AVPNATVFPTDD78 pKa = 4.72FITTAHH84 pKa = 5.95YY85 pKa = 10.57LKK87 pKa = 9.98TSTYY91 pKa = 9.02VQITGFFDD99 pKa = 3.13RR100 pKa = 11.84TKK102 pKa = 11.2YY103 pKa = 10.51DD104 pKa = 3.34LQEE107 pKa = 4.09TDD109 pKa = 4.72GGGQYY114 pKa = 10.89DD115 pKa = 3.6NHH117 pKa = 6.84AKK119 pKa = 10.07GKK121 pKa = 9.46PVGAQCKK128 pKa = 9.12GYY130 pKa = 10.66NYY132 pKa = 8.96FVNMIEE138 pKa = 4.38PDD140 pKa = 3.41LQRR143 pKa = 11.84FCIRR147 pKa = 11.84CCQDD151 pKa = 3.97KK152 pKa = 10.97DD153 pKa = 3.57DD154 pKa = 5.29CNTGRR159 pKa = 11.84SGYY162 pKa = 9.87GCLRR166 pKa = 11.84IITGDD171 pKa = 3.59YY172 pKa = 10.55TDD174 pKa = 4.86DD175 pKa = 3.59NNMIGNSTTSSGHH188 pKa = 5.7SNNHH192 pKa = 5.43INSVLAEE199 pKa = 4.21LDD201 pKa = 3.83EE202 pKa = 5.16LPAATDD208 pKa = 3.44STNATDD214 pKa = 3.64STNATDD220 pKa = 3.86STDD223 pKa = 3.39TATSADD229 pKa = 3.88TADD232 pKa = 3.79SSSTEE237 pKa = 4.19SNSALDD243 pKa = 3.98EE244 pKa = 4.64LDD246 pKa = 4.37DD247 pKa = 5.59LSVSDD252 pKa = 3.97STGSNDD258 pKa = 3.74TDD260 pKa = 3.63DD261 pKa = 4.78TGDD264 pKa = 3.76SDD266 pKa = 6.2DD267 pKa = 5.19SEE269 pKa = 4.6QSTDD273 pKa = 3.7DD274 pKa = 3.49TTEE277 pKa = 4.13DD278 pKa = 3.25ATEE281 pKa = 4.43EE282 pKa = 4.24PTSDD286 pKa = 3.09TTEE289 pKa = 3.92EE290 pKa = 4.38GTNDD294 pKa = 3.27DD295 pKa = 3.97TTATTASEE303 pKa = 4.31KK304 pKa = 10.09IANEE308 pKa = 3.63IQTLQSKK315 pKa = 10.35LSNQQSIDD323 pKa = 3.54EE324 pKa = 4.67VQTQWKK330 pKa = 7.54TFASQLSQDD339 pKa = 4.04YY340 pKa = 10.58PDD342 pKa = 3.88IATQISQLTNISSALTSSEE361 pKa = 3.85QLNTFYY367 pKa = 11.55GLVLTKK373 pKa = 10.79LQTFEE378 pKa = 4.66NNSTNTNTTATSDD391 pKa = 3.43PSATLTHH398 pKa = 6.82TNTQEE403 pKa = 4.57DD404 pKa = 4.93LDD406 pKa = 3.61WLYY409 pKa = 11.28NQRR412 pKa = 11.84EE413 pKa = 4.29SHH415 pKa = 7.15DD416 pKa = 4.05NQATWW421 pKa = 2.85

Molecular weight: 45.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1C6S7|I1C6S7_RHIO9 Uncharacterized protein OS=Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) OX=246409 GN=RO3G_08867 PE=4 SV=1
MM1 pKa = 7.64FGQLWTTATTRR12 pKa = 11.84FASLARR18 pKa = 11.84PNTMGSSILSSSLGAFRR35 pKa = 11.84NPLTTAFNTTTQMRR49 pKa = 11.84FISRR53 pKa = 11.84GNTYY57 pKa = 10.24QPSQLVRR64 pKa = 11.84KK65 pKa = 9.19RR66 pKa = 11.84RR67 pKa = 11.84HH68 pKa = 5.1GFLARR73 pKa = 11.84LATKK77 pKa = 10.41NGRR80 pKa = 11.84HH81 pKa = 4.42TLNRR85 pKa = 11.84RR86 pKa = 11.84RR87 pKa = 11.84MKK89 pKa = 10.31GRR91 pKa = 11.84KK92 pKa = 8.59NLSHH96 pKa = 7.01

Molecular weight: 10.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16971

0

16971

5840663

29

5940

344.2

39.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.339 ± 0.017

1.442 ± 0.008

5.608 ± 0.014

6.549 ± 0.03

4.018 ± 0.013

4.886 ± 0.018

2.592 ± 0.01

6.18 ± 0.016

6.793 ± 0.021

9.209 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.007

5.034 ± 0.017

4.756 ± 0.018

4.484 ± 0.017

5.038 ± 0.014

8.114 ± 0.023

5.93 ± 0.016

5.939 ± 0.02

1.231 ± 0.007

3.429 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski