Jatropha curcas (Barbados nut)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Jatropheae; Jatropha

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27058 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A067JYR6|A0A067JYR6_JATCU Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_26516 PE=4 SV=1
MM1 pKa = 7.38TSSDD5 pKa = 3.36LSGEE9 pKa = 4.33DD10 pKa = 3.61FLEE13 pKa = 4.23SLGISLDD20 pKa = 4.01DD21 pKa = 4.21VDD23 pKa = 5.07LTTDD27 pKa = 3.2ADD29 pKa = 4.19TYY31 pKa = 11.66ASVTGIFAQSLEE43 pKa = 4.18NMAHH47 pKa = 6.28LAEE50 pKa = 4.6YY51 pKa = 9.94DD52 pKa = 3.13WAGAILSRR60 pKa = 11.84MYY62 pKa = 11.41DD63 pKa = 3.47DD64 pKa = 4.76MSDD67 pKa = 3.79LSRR70 pKa = 11.84GHH72 pKa = 6.61CKK74 pKa = 10.62LSGTYY79 pKa = 9.14YY80 pKa = 9.15FWEE83 pKa = 4.41VEE85 pKa = 4.34VGAMTAGLQSWIQVDD100 pKa = 3.54SHH102 pKa = 5.82FQRR105 pKa = 11.84APRR108 pKa = 11.84AISEE112 pKa = 4.28ASGPVHH118 pKa = 7.35PDD120 pKa = 2.99LANLRR125 pKa = 11.84LPYY128 pKa = 9.99SIPYY132 pKa = 8.57YY133 pKa = 10.25PPDD136 pKa = 4.18GPPALWEE143 pKa = 4.11VSLEE147 pKa = 4.34SVDD150 pKa = 5.14RR151 pKa = 11.84PALPSEE157 pKa = 5.54DD158 pKa = 3.2ITEE161 pKa = 4.31VPVGLVNQMIEE172 pKa = 4.4LMLGMQQEE180 pKa = 4.38LTAAGLIGPLTIRR193 pKa = 11.84GGGGPVADD201 pKa = 4.1SCC203 pKa = 5.47

Molecular weight: 21.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A067JDP7|A0A067JDP7_JATCU Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_03197 PE=4 SV=1
MM1 pKa = 7.7PIWYY5 pKa = 9.44LKK7 pKa = 10.51RR8 pKa = 11.84PGRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 10.37DD14 pKa = 3.1RR15 pKa = 11.84GTGPEE20 pKa = 4.02IFFGGPKK27 pKa = 9.95LPKK30 pKa = 9.85KK31 pKa = 9.97PPEE34 pKa = 3.81RR35 pKa = 11.84KK36 pKa = 8.72IRR38 pKa = 11.84RR39 pKa = 11.84SAGRR43 pKa = 11.84GGGSSRR49 pKa = 11.84PSRR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84WQQPPFTAAVAAAALHH70 pKa = 6.02GGGGSSRR77 pKa = 11.84PSRR80 pKa = 11.84RR81 pKa = 11.84RR82 pKa = 11.84WQQPPFTAAVAAAALHH98 pKa = 6.02GGGGSSRR105 pKa = 11.84PSRR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84WQQPPFTAAVAAAALHH126 pKa = 6.02GGGGSSRR133 pKa = 11.84PSRR136 pKa = 11.84RR137 pKa = 11.84RR138 pKa = 11.84WQQPPFTAAVAAAALHH154 pKa = 6.02GGGGSSRR161 pKa = 11.84PSRR164 pKa = 11.84RR165 pKa = 11.84RR166 pKa = 11.84WQQPPFTAAVAAAALHH182 pKa = 6.02GGGGSSRR189 pKa = 11.84PSRR192 pKa = 11.84RR193 pKa = 11.84RR194 pKa = 11.84WQQPPFTAAVAAAALHH210 pKa = 6.02GGGGSSRR217 pKa = 11.84PSRR220 pKa = 11.84RR221 pKa = 11.84RR222 pKa = 11.84WQQPPFTAAVAAAALHH238 pKa = 6.02GGGGSSRR245 pKa = 11.84PSRR248 pKa = 11.84RR249 pKa = 11.84RR250 pKa = 11.84WQQPPFTAAVAAAALHH266 pKa = 6.02GGGGSSRR273 pKa = 11.84PSRR276 pKa = 11.84RR277 pKa = 11.84RR278 pKa = 11.84WQQPPFTAAVAAAALHH294 pKa = 6.02GGGGSSRR301 pKa = 11.84PSRR304 pKa = 11.84RR305 pKa = 11.84RR306 pKa = 11.84WQQPPFTAAVAAAALHH322 pKa = 6.02GGGGSSRR329 pKa = 11.84PSRR332 pKa = 11.84RR333 pKa = 11.84RR334 pKa = 11.84WQQPPFTAAVAAAALHH350 pKa = 6.02GGGGSSRR357 pKa = 11.84PSRR360 pKa = 11.84RR361 pKa = 11.84RR362 pKa = 11.84WQQPPFTAAVAAAALHH378 pKa = 6.02GGGGSSRR385 pKa = 11.84PSRR388 pKa = 11.84RR389 pKa = 11.84RR390 pKa = 11.84WQQPPFTAAVAAAALHH406 pKa = 6.02GGGGSSRR413 pKa = 11.84PSRR416 pKa = 11.84RR417 pKa = 11.84RR418 pKa = 11.84WQQPPFTAAVAAAALHH434 pKa = 6.02GGGGSSRR441 pKa = 11.84PSRR444 pKa = 11.84RR445 pKa = 11.84RR446 pKa = 11.84WQQPPFTAAVAAAALHH462 pKa = 6.02GGGGSSRR469 pKa = 11.84PSRR472 pKa = 11.84RR473 pKa = 11.84RR474 pKa = 11.84WQQPPFTAAVAAAALHH490 pKa = 6.02GGGGSSRR497 pKa = 11.84PSRR500 pKa = 11.84RR501 pKa = 11.84RR502 pKa = 11.84WQQPPFTAAVAAAALHH518 pKa = 6.02GGGGSSRR525 pKa = 11.84PSRR528 pKa = 11.84RR529 pKa = 11.84RR530 pKa = 11.84WQQPPFTAAVAAAALHH546 pKa = 6.02GGGGSSRR553 pKa = 11.84PSRR556 pKa = 11.84RR557 pKa = 11.84RR558 pKa = 11.84WQQPPFTAAVAAAALHH574 pKa = 6.02GGGGSSRR581 pKa = 11.84PSRR584 pKa = 11.84RR585 pKa = 11.84RR586 pKa = 11.84WQQPPFTAAVAAAALHH602 pKa = 6.02GGGGSSRR609 pKa = 11.84PSRR612 pKa = 11.84RR613 pKa = 11.84RR614 pKa = 11.84WQQPRR619 pKa = 11.84LVLQHH624 pKa = 7.02DD625 pKa = 4.16IVSGSTSGSS634 pKa = 3.26

Molecular weight: 65.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27058

0

27058

9912802

50

5587

366.4

40.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.772 ± 0.013

1.808 ± 0.009

5.284 ± 0.011

6.615 ± 0.018

4.205 ± 0.009

6.525 ± 0.018

2.315 ± 0.007

5.472 ± 0.012

6.091 ± 0.016

9.673 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.006

4.515 ± 0.01

5.017 ± 0.015

3.715 ± 0.01

5.374 ± 0.013

8.834 ± 0.017

4.905 ± 0.01

6.296 ± 0.01

1.305 ± 0.006

2.854 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski