Comamonas serinivorans
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3699 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y0EP99|A0A1Y0EP99_9BURK Cytochrome C OS=Comamonas serinivorans OX=1082851 GN=CCO03_11000 PE=4 SV=1
MM1 pKa = 7.5 QILFPNSFGHH11 pKa = 6.97 PLPQEE16 pKa = 4.61 DD17 pKa = 4.43 EE18 pKa = 4.36 VLDD21 pKa = 3.96 LQEE24 pKa = 4.51 QYY26 pKa = 11.54 GFSQDD31 pKa = 3.25 YY32 pKa = 11.43 AEE34 pKa = 5.37 FLFRR38 pKa = 11.84 QNGFSFDD45 pKa = 3.79 RR46 pKa = 11.84 LADD49 pKa = 3.63 ASDD52 pKa = 4.07 PDD54 pKa = 3.25 EE55 pKa = 4.54 CLAVGEE61 pKa = 5.07 DD62 pKa = 3.96 DD63 pKa = 5.32 AEE65 pKa = 4.48 GNADD69 pKa = 4.02 LRR71 pKa = 11.84 HH72 pKa = 6.23 LYY74 pKa = 10.77 ALGAEE79 pKa = 4.5 APFDD83 pKa = 4.09 DD84 pKa = 6.22 LNAQLEE90 pKa = 4.34 DD91 pKa = 4.48 FIFRR95 pKa = 11.84 EE96 pKa = 4.09 VLFPIGAGYY105 pKa = 9.87 GGQVFVEE112 pKa = 4.32 VLAGKK117 pKa = 9.25 YY118 pKa = 8.81 QGFIASLDD126 pKa = 3.7 HH127 pKa = 6.86 EE128 pKa = 5.27 LYY130 pKa = 10.88 ASNTSLEE137 pKa = 4.29 EE138 pKa = 3.96 YY139 pKa = 10.7 LEE141 pKa = 4.16 EE142 pKa = 6.17 AEE144 pKa = 5.03 LEE146 pKa = 4.19 GSEE149 pKa = 4.3 EE150 pKa = 4.64 DD151 pKa = 4.25 RR152 pKa = 11.84 DD153 pKa = 3.98 ALADD157 pKa = 3.82 ALCDD161 pKa = 4.06 PEE163 pKa = 6.44 SGLAWFHH170 pKa = 6.25 ARR172 pKa = 11.84 SLRR175 pKa = 11.84 QFLSEE180 pKa = 5.22 CVFCDD185 pKa = 3.38 EE186 pKa = 4.59 SLTGFVMDD194 pKa = 5.92 ADD196 pKa = 4.43 DD197 pKa = 6.69 LPDD200 pKa = 3.58 EE201 pKa = 4.7 LLNN204 pKa = 4.27
Molecular weight: 22.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.643
IPC_protein 3.643
Toseland 3.439
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.541
Rodwell 3.478
Grimsley 3.35
Solomon 3.617
Lehninger 3.579
Nozaki 3.745
DTASelect 3.935
Thurlkill 3.49
EMBOSS 3.554
Sillero 3.77
Patrickios 0.807
IPC_peptide 3.617
IPC2_peptide 3.745
IPC2.peptide.svr19 3.697
Protein with the highest isoelectric point:
>tr|A0A1Y0ES83|A0A1Y0ES83_9BURK Probable GTP-binding protein EngB OS=Comamonas serinivorans OX=1082851 GN=engB PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.25 QPSKK9 pKa = 7.79 TRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.79 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.7 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3699
0
3699
1213414
34
2774
328.0
35.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.774 ± 0.06
0.926 ± 0.012
5.236 ± 0.03
4.745 ± 0.036
3.197 ± 0.024
8.409 ± 0.045
2.413 ± 0.023
3.752 ± 0.032
2.838 ± 0.037
10.861 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.364 ± 0.02
2.438 ± 0.024
5.718 ± 0.035
4.66 ± 0.029
6.823 ± 0.041
5.137 ± 0.031
5.295 ± 0.033
7.768 ± 0.035
1.625 ± 0.02
2.021 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here