Sphingomonas sp. L-1-4w-11

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U1L2D7|A0A4U1L2D7_9SPHN 2-polyprenylphenol 6-hydroxylase OS=Sphingomonas sp. L-1-4w-11 OX=2572576 GN=ubiB PE=4 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84FSTPSLAALSLMLFAAPAYY21 pKa = 9.39AQEE24 pKa = 4.31EE25 pKa = 4.46TAPPEE30 pKa = 4.22PLTVTGSVTLASDD43 pKa = 3.34YY44 pKa = 10.82RR45 pKa = 11.84FRR47 pKa = 11.84GVSQTDD53 pKa = 3.26KK54 pKa = 11.04EE55 pKa = 4.23FAIQGGATIAHH66 pKa = 6.66EE67 pKa = 4.45SGVYY71 pKa = 10.21AGFWGSNLAGWGTFGGANMEE91 pKa = 4.02LDD93 pKa = 3.11IYY95 pKa = 11.17GGFKK99 pKa = 10.65FPVGDD104 pKa = 4.29GGTLDD109 pKa = 5.14VGVTWYY115 pKa = 8.57MYY117 pKa = 10.39PGGADD122 pKa = 3.12VTDD125 pKa = 3.98FAEE128 pKa = 5.02PYY130 pKa = 10.14VKK132 pKa = 10.67LSGTAGPVSLTAGVFYY148 pKa = 10.72APKK151 pKa = 10.16QEE153 pKa = 4.07ALGDD157 pKa = 3.85VFFTGAAAASGIPDD171 pKa = 4.22DD172 pKa = 5.5PGDD175 pKa = 4.36SEE177 pKa = 5.69DD178 pKa = 3.93NLYY181 pKa = 11.18LSGDD185 pKa = 3.49AVFAIPEE192 pKa = 4.48TPVSLRR198 pKa = 11.84GHH200 pKa = 6.71IGYY203 pKa = 9.99SDD205 pKa = 4.0GNPGLGPNGTSVAPTGQYY223 pKa = 9.57WDD225 pKa = 3.71WNLGADD231 pKa = 3.8VSVLGLTLGVSYY243 pKa = 10.94IDD245 pKa = 3.83TDD247 pKa = 3.28ISAADD252 pKa = 3.76GAYY255 pKa = 10.77LLPNFQKK262 pKa = 8.08TTGGSIADD270 pKa = 3.66GTIVVSLTAAFF281 pKa = 3.94

Molecular weight: 28.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U1L0N4|A0A4U1L0N4_9SPHN Uncharacterized protein OS=Sphingomonas sp. L-1-4w-11 OX=2572576 GN=FBR43_05690 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.82GFFARR21 pKa = 11.84MATAAGRR28 pKa = 11.84KK29 pKa = 8.9VIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3108

0

3108

998105

29

4506

321.1

34.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.954 ± 0.084

0.739 ± 0.013

6.111 ± 0.043

5.318 ± 0.04

3.401 ± 0.028

9.05 ± 0.045

1.961 ± 0.022

4.779 ± 0.023

2.233 ± 0.038

9.867 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.36 ± 0.021

2.249 ± 0.03

5.464 ± 0.03

2.895 ± 0.025

8.121 ± 0.047

4.603 ± 0.031

5.147 ± 0.036

7.286 ± 0.031

1.412 ± 0.02

2.049 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski