Streptomyces jumonjinensis
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7035 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A646KFI6|A0A646KFI6_STRJU Dihydrolipoyl dehydrogenase OS=Streptomyces jumonjinensis OX=1945 GN=lpdA PE=3 SV=1
MM1 pKa = 7.05 TAQHH5 pKa = 6.3 EE6 pKa = 4.45 APSDD10 pKa = 3.67 GAAEE14 pKa = 4.07 ALEE17 pKa = 4.04 VWIDD21 pKa = 3.41 QDD23 pKa = 3.99 LCTGDD28 pKa = 4.65 GICVQYY34 pKa = 11.21 APDD37 pKa = 3.85 VFEE40 pKa = 6.18 LDD42 pKa = 3.22 IDD44 pKa = 3.57 GLAYY48 pKa = 10.48 VKK50 pKa = 11.02 SPDD53 pKa = 4.9 DD54 pKa = 4.64 EE55 pKa = 5.2 LLQDD59 pKa = 4.6 PGATAPVPLPLLRR72 pKa = 11.84 DD73 pKa = 3.56 VVDD76 pKa = 4.29 SVKK79 pKa = 10.35 EE80 pKa = 3.97 CPGDD84 pKa = 4.13 CIHH87 pKa = 6.28 VRR89 pKa = 11.84 RR90 pKa = 11.84 VSDD93 pKa = 3.47 SVEE96 pKa = 3.98 VYY98 pKa = 10.88 GPDD101 pKa = 3.29 VEE103 pKa = 4.49
Molecular weight: 11.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.694
IPC_protein 3.681
Toseland 3.465
ProMoST 3.834
Dawson 3.681
Bjellqvist 3.884
Wikipedia 3.643
Rodwell 3.503
Grimsley 3.376
Solomon 3.668
Lehninger 3.63
Nozaki 3.808
DTASelect 4.05
Thurlkill 3.528
EMBOSS 3.643
Sillero 3.808
Patrickios 0.896
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.766
Protein with the highest isoelectric point:
>tr|A0A646KE69|A0A646KE69_STRJU Response regulator OS=Streptomyces jumonjinensis OX=1945 GN=FF041_08950 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILASRR35 pKa = 11.84 RR36 pKa = 11.84 TKK38 pKa = 10.46 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSAA45 pKa = 3.91
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.676
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.398
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7035
0
7035
2247349
21
3239
319.5
34.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.594 ± 0.04
0.819 ± 0.007
5.894 ± 0.024
5.828 ± 0.028
2.675 ± 0.014
9.577 ± 0.031
2.275 ± 0.016
3.336 ± 0.017
2.052 ± 0.026
10.258 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.738 ± 0.012
1.7 ± 0.016
6.293 ± 0.029
2.706 ± 0.019
8.451 ± 0.028
5.207 ± 0.02
5.97 ± 0.024
8.034 ± 0.025
1.521 ± 0.013
2.072 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here