Sorangium cellulosum (strain So ce56) (Polyangium cellulosum (strain So ce56))
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9320 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A9GAX1|A9GAX1_SORC5 Uncharacterized protein OS=Sorangium cellulosum (strain So ce56) OX=448385 GN=sce2764 PE=4 SV=1
MM1 pKa = 7.56 RR2 pKa = 11.84 KK3 pKa = 8.96 SWLAVMFSVASMACLTSGCSALPDD27 pKa = 3.89 GDD29 pKa = 4.48 DD30 pKa = 3.8 PRR32 pKa = 11.84 TSEE35 pKa = 4.58 PVGTSQQPLVYY46 pKa = 9.54 GWSNRR51 pKa = 11.84 YY52 pKa = 9.46 GDD54 pKa = 4.27 INYY57 pKa = 8.92 QHH59 pKa = 7.03 AGDD62 pKa = 3.93 VTVDD66 pKa = 3.22 VSDD69 pKa = 5.05 NIIITAGGEE78 pKa = 4.14 GSVDD82 pKa = 4.03 FGGGAHH88 pKa = 6.28 TPTFYY93 pKa = 11.47 GVMLAKK99 pKa = 10.27 FDD101 pKa = 5.42 DD102 pKa = 4.28 SGNCIWSKK110 pKa = 9.81 MFEE113 pKa = 4.48 GVNLMGVATDD123 pKa = 3.36 SAGNVNIVGTMFGAIDD139 pKa = 3.87 FGGGPMSPLGMSDD152 pKa = 2.85 IFVAQFDD159 pKa = 3.71 ASGNYY164 pKa = 7.59 VWSDD168 pKa = 3.17 RR169 pKa = 11.84 FGGGPLEE176 pKa = 4.37 YY177 pKa = 10.8 AEE179 pKa = 4.52 GMAIAVDD186 pKa = 3.62 SSDD189 pKa = 3.44 NVIITGNYY197 pKa = 8.98 SGNLRR202 pKa = 11.84 FGSDD206 pKa = 3.07 FGIAVSNDD214 pKa = 2.74 VFLAKK219 pKa = 10.03 LDD221 pKa = 3.83 SSGNPVFGRR230 pKa = 11.84 FFGVPLVPGTGTGRR244 pKa = 11.84 AVAIDD249 pKa = 3.89 GSDD252 pKa = 3.69 NIVISGWYY260 pKa = 8.21 YY261 pKa = 10.85 GSMDD265 pKa = 3.99 FGGGPTPLVSVPDD278 pKa = 3.67 TADD281 pKa = 3.12 AFVAEE286 pKa = 4.85 FDD288 pKa = 4.49 SSGTYY293 pKa = 10.3 LWDD296 pKa = 3.42 TTFGNSGEE304 pKa = 4.04 ADD306 pKa = 3.73 ALNVATDD313 pKa = 3.41 SLGNVAVVGSFTGNMDD329 pKa = 2.89 IGGSTLTSAGNTDD342 pKa = 2.85 IFVAQFDD349 pKa = 3.85 NSGTHH354 pKa = 5.84 LWSHH358 pKa = 6.27 GFGDD362 pKa = 4.55 TDD364 pKa = 3.11 WDD366 pKa = 3.8 MASGVSINDD375 pKa = 3.39 SGDD378 pKa = 3.31 VFVTGDD384 pKa = 2.75 IGGAVDD390 pKa = 4.7 FGGGTLTSAGSSDD403 pKa = 3.6 VFVAHH408 pKa = 6.81 FDD410 pKa = 3.59 GSGTHH415 pKa = 5.77 VWSEE419 pKa = 4.23 NYY421 pKa = 10.62 GDD423 pKa = 4.26 TDD425 pKa = 3.77 PQGGSSIKK433 pKa = 9.82 IDD435 pKa = 3.66 SNGNAVVTGAFFGSVNFGGTTLTDD459 pKa = 3.47 AGTGDD464 pKa = 3.03 IFLAQFTPP472 pKa = 3.74
Molecular weight: 48.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.579
IPC_protein 3.643
Toseland 3.389
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.643
Rodwell 3.452
Grimsley 3.287
Solomon 3.656
Lehninger 3.605
Nozaki 3.77
DTASelect 4.101
Thurlkill 3.452
EMBOSS 3.643
Sillero 3.77
Patrickios 1.252
IPC_peptide 3.643
IPC2_peptide 3.732
IPC2.peptide.svr19 3.711
Protein with the highest isoelectric point:
>tr|A9FTL4|A9FTL4_SORC5 CENP-V/GFA domain-containing protein OS=Sorangium cellulosum (strain So ce56) OX=448385 GN=sce8373 PE=3 SV=1
MM1 pKa = 6.99 SHH3 pKa = 6.76 ARR5 pKa = 11.84 PVWSRR10 pKa = 11.84 HH11 pKa = 4.45 RR12 pKa = 11.84 SAPGPGTRR20 pKa = 11.84 SRR22 pKa = 11.84 IHH24 pKa = 6.01 TRR26 pKa = 11.84 DD27 pKa = 3.29 ASEE30 pKa = 4.01 QGPPATSTPVGWQGRR45 pKa = 11.84 LAGPPQPRR53 pKa = 11.84 SGWLAGPGPPQSRR66 pKa = 11.84 SGPPNLGRR74 pKa = 11.84 VRR76 pKa = 11.84 LNLGRR81 pKa = 11.84 VRR83 pKa = 11.84 LNLGRR88 pKa = 11.84 VRR90 pKa = 11.84 LNLGRR95 pKa = 11.84 VRR97 pKa = 11.84 LNLGRR102 pKa = 11.84 VRR104 pKa = 11.84 LSLGRR109 pKa = 11.84 GRR111 pKa = 11.84 LNLGRR116 pKa = 11.84 GRR118 pKa = 11.84 LNLGRR123 pKa = 11.84 VRR125 pKa = 11.84 LNLGRR130 pKa = 11.84 VRR132 pKa = 11.84 LNLGRR137 pKa = 11.84 VRR139 pKa = 11.84 LNLGRR144 pKa = 11.84 VHH146 pKa = 7.5 PARR149 pKa = 11.84 ASAVAGGAAALTCAAGAPIPPHH171 pKa = 5.61 LQRR174 pKa = 11.84 TAARR178 pKa = 11.84 TGGSRR183 pKa = 11.84 RR184 pKa = 11.84 PPSPLDD190 pKa = 3.53 LSPEE194 pKa = 4.01 LSDD197 pKa = 3.77 ALARR201 pKa = 11.84 SIPLPPTEE209 pKa = 4.19 PWRR212 pKa = 11.84 KK213 pKa = 9.4 RR214 pKa = 11.84 SS215 pKa = 3.38
Molecular weight: 23.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.37
IPC2_protein 10.804
IPC_protein 12.413
Toseland 12.574
ProMoST 13.071
Dawson 12.574
Bjellqvist 12.574
Wikipedia 13.056
Rodwell 12.091
Grimsley 12.618
Solomon 13.071
Lehninger 12.969
Nozaki 12.574
DTASelect 12.574
Thurlkill 12.574
EMBOSS 13.071
Sillero 12.574
Patrickios 11.798
IPC_peptide 13.071
IPC2_peptide 12.062
IPC2.peptide.svr19 9.179
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9320
0
9320
3743060
30
8417
401.6
42.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.161 ± 0.046
1.185 ± 0.019
5.674 ± 0.022
6.237 ± 0.022
3.041 ± 0.014
9.362 ± 0.034
2.044 ± 0.011
3.601 ± 0.015
2.334 ± 0.019
10.131 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.702 ± 0.01
1.759 ± 0.015
6.369 ± 0.025
2.677 ± 0.018
8.625 ± 0.03
5.707 ± 0.019
4.753 ± 0.021
7.449 ± 0.022
1.269 ± 0.011
1.92 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here