Thermosinus carboxydivorans Nor1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Sporomusaceae; Thermosinus; Thermosinus carboxydivorans

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2738 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A1HQ97|A1HQ97_9FIRM Cysteine synthase A OS=Thermosinus carboxydivorans Nor1 OX=401526 GN=TcarDRAFT_1113 PE=4 SV=1
MM1 pKa = 7.7ANLKK5 pKa = 10.38EE6 pKa = 4.16KK7 pKa = 10.51VAYY10 pKa = 10.0LQGLTKK16 pKa = 10.46GLNVDD21 pKa = 3.67TQSPEE26 pKa = 3.53GKK28 pKa = 10.08LLVNIVDD35 pKa = 3.98VLDD38 pKa = 4.03SFAEE42 pKa = 4.27EE43 pKa = 4.17WACMSVAQQEE53 pKa = 4.43LEE55 pKa = 4.29NYY57 pKa = 10.04VEE59 pKa = 4.78TIDD62 pKa = 6.15DD63 pKa = 4.74DD64 pKa = 4.22LTDD67 pKa = 5.29LEE69 pKa = 4.98DD70 pKa = 3.21EE71 pKa = 4.59VYY73 pKa = 10.24DD74 pKa = 4.12VKK76 pKa = 10.87MVDD79 pKa = 3.56DD80 pKa = 5.32AEE82 pKa = 4.4IADD85 pKa = 4.02EE86 pKa = 6.07DD87 pKa = 4.06EE88 pKa = 5.4DD89 pKa = 4.45EE90 pKa = 5.51DD91 pKa = 4.12MVEE94 pKa = 4.4VEE96 pKa = 5.7CPICHH101 pKa = 6.84EE102 pKa = 4.53DD103 pKa = 3.37VTFEE107 pKa = 5.14ADD109 pKa = 5.77LLDD112 pKa = 4.03EE113 pKa = 5.34DD114 pKa = 5.47DD115 pKa = 4.68PVEE118 pKa = 4.52VTCPHH123 pKa = 6.84CGGIVYY129 pKa = 10.58DD130 pKa = 4.01NTLDD134 pKa = 3.75FADD137 pKa = 5.83DD138 pKa = 3.46EE139 pKa = 4.64MEE141 pKa = 4.16DD142 pKa = 3.75MRR144 pKa = 11.84SGRR147 pKa = 11.84HH148 pKa = 5.36LLHH151 pKa = 7.01PGVV154 pKa = 3.9

Molecular weight: 17.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A1HQX2|A1HQX2_9FIRM 4Fe-4S ferredoxin iron-sulfur binding domain protein OS=Thermosinus carboxydivorans Nor1 OX=401526 GN=TcarDRAFT_1298 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 10.54RR4 pKa = 11.84KK5 pKa = 9.56LRR7 pKa = 11.84LLVEE11 pKa = 4.29KK12 pKa = 10.34GYY14 pKa = 11.31VSGWDD19 pKa = 3.83DD20 pKa = 3.38PRR22 pKa = 11.84MPTISGLRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84GYY34 pKa = 8.4TPEE37 pKa = 5.22AIRR40 pKa = 11.84DD41 pKa = 3.57FCDD44 pKa = 3.97RR45 pKa = 11.84IGVAKK50 pKa = 10.24SKK52 pKa = 9.67QHH54 pKa = 6.12GRR56 pKa = 11.84YY57 pKa = 9.3CPTGTLHH64 pKa = 6.87SRR66 pKa = 11.84RR67 pKa = 11.84SS68 pKa = 3.4

Molecular weight: 7.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2738

0

2738

821021

24

1814

299.9

32.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.642 ± 0.066

1.149 ± 0.022

4.886 ± 0.034

6.059 ± 0.054

3.639 ± 0.031

7.893 ± 0.05

1.895 ± 0.017

6.859 ± 0.035

5.154 ± 0.048

10.194 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.594 ± 0.022

3.292 ± 0.029

4.403 ± 0.027

3.485 ± 0.03

5.793 ± 0.05

4.543 ± 0.029

5.261 ± 0.026

8.128 ± 0.047

1.01 ± 0.018

3.12 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski