Hippea maritima (strain ATCC 700847 / DSM 10411 / MH2)
Average proteome isoelectric point is 7.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1653 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2LXQ7|F2LXQ7_HIPMA Uncharacterized protein OS=Hippea maritima (strain ATCC 700847 / DSM 10411 / MH2) OX=760142 GN=Hipma_1342 PE=4 SV=1
MM1 pKa = 7.62 KK2 pKa = 10.03 EE3 pKa = 3.7 LYY5 pKa = 10.16 DD6 pKa = 3.71 VLFHH10 pKa = 7.19 KK11 pKa = 11.13 GEE13 pKa = 4.11 LLVEE17 pKa = 3.96 QGEE20 pKa = 4.57 CEE22 pKa = 3.82 KK23 pKa = 11.14 AIVYY27 pKa = 7.63 FLNAVKK33 pKa = 10.17 EE34 pKa = 4.26 NPSFEE39 pKa = 4.18 DD40 pKa = 3.77 AYY42 pKa = 10.3 IEE44 pKa = 4.58 IGYY47 pKa = 10.29 CYY49 pKa = 10.5 AQLDD53 pKa = 4.12 NFDD56 pKa = 4.7 DD57 pKa = 5.53 AEE59 pKa = 4.56 DD60 pKa = 3.98 YY61 pKa = 10.87 CNKK64 pKa = 10.38 AIEE67 pKa = 4.31 INPNNLEE74 pKa = 4.19 AYY76 pKa = 7.54 NTLAMIYY83 pKa = 9.68 HH84 pKa = 6.86 KK85 pKa = 10.69 FGFFEE90 pKa = 5.16 DD91 pKa = 4.15 EE92 pKa = 3.98 IEE94 pKa = 4.25 ALNEE98 pKa = 3.67 IIIRR102 pKa = 11.84 LDD104 pKa = 3.37 EE105 pKa = 5.06 PDD107 pKa = 3.1 ASIYY111 pKa = 10.99 LNIGNAYY118 pKa = 10.04 YY119 pKa = 10.56 EE120 pKa = 4.24 LGEE123 pKa = 3.97 NDD125 pKa = 3.63 RR126 pKa = 11.84 AIEE129 pKa = 4.55 FYY131 pKa = 11.63 DD132 pKa = 3.33 MAIGMEE138 pKa = 4.16 PDD140 pKa = 3.24 FAEE143 pKa = 5.5 AYY145 pKa = 10.68 ANMGNAYY152 pKa = 7.51 MAKK155 pKa = 10.18 DD156 pKa = 3.93 EE157 pKa = 4.54 YY158 pKa = 10.95 IKK160 pKa = 10.53 ATEE163 pKa = 4.45 AYY165 pKa = 8.91 KK166 pKa = 10.38 QALQIDD172 pKa = 4.66 PNMSDD177 pKa = 3.06 VYY179 pKa = 11.03 LNLGIVYY186 pKa = 10.21 GEE188 pKa = 4.0 LGSYY192 pKa = 10.76 DD193 pKa = 3.55 EE194 pKa = 4.13 AVKK197 pKa = 10.92 YY198 pKa = 9.94 FEE200 pKa = 3.73 QSIRR204 pKa = 11.84 INPYY208 pKa = 9.94 NPSAHH213 pKa = 6.14 YY214 pKa = 9.99 NLGIIWVMLNEE225 pKa = 4.29 KK226 pKa = 10.06 EE227 pKa = 4.23 KK228 pKa = 11.1 ALNEE232 pKa = 3.85 YY233 pKa = 10.06 EE234 pKa = 4.44 RR235 pKa = 11.84 LKK237 pKa = 11.17 NLNKK241 pKa = 10.41 DD242 pKa = 3.38 LALSLVQLIDD252 pKa = 3.37 KK253 pKa = 9.93 EE254 pKa = 4.79 SYY256 pKa = 10.54 KK257 pKa = 10.35 FRR259 pKa = 11.84 QQ260 pKa = 3.23
Molecular weight: 30.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.041
IPC2_protein 4.253
IPC_protein 4.202
Toseland 4.024
ProMoST 4.304
Dawson 4.139
Bjellqvist 4.291
Wikipedia 3.999
Rodwell 4.024
Grimsley 3.935
Solomon 4.139
Lehninger 4.088
Nozaki 4.24
DTASelect 4.38
Thurlkill 4.037
EMBOSS 4.024
Sillero 4.304
Patrickios 0.693
IPC_peptide 4.139
IPC2_peptide 4.291
IPC2.peptide.svr19 4.195
Protein with the highest isoelectric point:
>tr|F2LWX0|F2LWX0_HIPMA Glutaredoxin-like protein YruB-family OS=Hippea maritima (strain ATCC 700847 / DSM 10411 / MH2) OX=760142 GN=Hipma_1192 PE=4 SV=1
MM1 pKa = 7.38 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPHH8 pKa = 5.21 NKK10 pKa = 8.23 PRR12 pKa = 11.84 KK13 pKa = 7.28 RR14 pKa = 11.84 THH16 pKa = 6.07 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 MKK23 pKa = 8.41 TAGGRR28 pKa = 11.84 KK29 pKa = 8.24 VLSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.84 GRR39 pKa = 11.84 KK40 pKa = 8.55 RR41 pKa = 11.84 LSVV44 pKa = 3.2
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.522
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.735
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.457
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.169
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1653
0
1653
516646
35
1584
312.6
35.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.496 ± 0.053
0.941 ± 0.024
5.5 ± 0.048
6.831 ± 0.064
5.203 ± 0.048
6.476 ± 0.054
1.506 ± 0.021
8.985 ± 0.062
9.406 ± 0.071
9.511 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.298 ± 0.028
4.942 ± 0.052
3.349 ± 0.034
2.333 ± 0.03
4.123 ± 0.041
6.121 ± 0.053
4.473 ± 0.045
6.888 ± 0.049
0.704 ± 0.015
3.915 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here