Halolactibacillus miurensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Halolactibacillus

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3011 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I6SCH3|A0A1I6SCH3_9BACI Lysophospholipase alpha-beta hydrolase superfamily OS=Halolactibacillus miurensis OX=306541 GN=SAMN05421668_10879 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.41KK3 pKa = 9.96IFTLLLILMLGVLAACGGDD22 pKa = 3.52DD23 pKa = 4.68TSNDD27 pKa = 3.51DD28 pKa = 3.82TGGSDD33 pKa = 3.91SDD35 pKa = 3.83NGTDD39 pKa = 4.34SGEE42 pKa = 4.28TSDD45 pKa = 4.91GVQSVTAWAWDD56 pKa = 3.53PNFNIRR62 pKa = 11.84ALEE65 pKa = 4.09LANQAYY71 pKa = 10.54DD72 pKa = 3.99GDD74 pKa = 3.99SEE76 pKa = 4.73MYY78 pKa = 10.76VEE80 pKa = 5.18IIEE83 pKa = 4.3NAQDD87 pKa = 5.55DD88 pKa = 4.38IIQRR92 pKa = 11.84LNAGLSSGTTQGMPNIVLIEE112 pKa = 4.37DD113 pKa = 3.41YY114 pKa = 10.36RR115 pKa = 11.84AQSFLQSYY123 pKa = 9.77PDD125 pKa = 3.33AFYY128 pKa = 10.81PLTDD132 pKa = 4.51YY133 pKa = 11.31INPDD137 pKa = 3.18DD138 pKa = 3.84FAQYY142 pKa = 10.33KK143 pKa = 9.65VEE145 pKa = 4.01ATSFDD150 pKa = 3.21GDD152 pKa = 3.47IYY154 pKa = 11.22GLPFDD159 pKa = 4.35TGVSGLYY166 pKa = 10.3VRR168 pKa = 11.84TDD170 pKa = 3.52YY171 pKa = 11.53LEE173 pKa = 4.16EE174 pKa = 5.52AGYY177 pKa = 8.13TVEE180 pKa = 5.12DD181 pKa = 4.4LSDD184 pKa = 3.74ITWKK188 pKa = 10.63EE189 pKa = 3.63YY190 pKa = 10.57IEE192 pKa = 4.16IGKK195 pKa = 8.45EE196 pKa = 3.73VKK198 pKa = 10.27AATGKK203 pKa = 10.66DD204 pKa = 4.07MITLDD209 pKa = 4.03PNDD212 pKa = 3.76FGMVRR217 pKa = 11.84MMLQSAGEE225 pKa = 4.14WYY227 pKa = 10.54FEE229 pKa = 4.25EE230 pKa = 5.86DD231 pKa = 3.58GNTPALADD239 pKa = 3.62NPALEE244 pKa = 4.97EE245 pKa = 3.91IFVLYY250 pKa = 10.63KK251 pKa = 11.1EE252 pKa = 4.67MMDD255 pKa = 3.73ADD257 pKa = 3.84IVKK260 pKa = 10.5LNSDD264 pKa = 3.29WSQFVSAFNSGDD276 pKa = 3.47VATVPTGNWITPSVKK291 pKa = 10.57AEE293 pKa = 3.85ASQSGNWAVLPLPRR307 pKa = 11.84LEE309 pKa = 3.99VDD311 pKa = 3.55GAVNTSNLGGSSWYY325 pKa = 10.4VLNQDD330 pKa = 3.65GKK332 pKa = 9.71EE333 pKa = 3.77LAAEE337 pKa = 4.52FLANTFGSNEE347 pKa = 3.71QFYY350 pKa = 10.19TDD352 pKa = 4.1LVTEE356 pKa = 4.69IGALGTYY363 pKa = 9.91IPGTQGEE370 pKa = 4.44AFEE373 pKa = 4.65QEE375 pKa = 4.32DD376 pKa = 4.51PFFGGQAIIQDD387 pKa = 4.0FATWSEE393 pKa = 4.06QIPPVNYY400 pKa = 10.22GMHH403 pKa = 6.74TYY405 pKa = 10.8AVDD408 pKa = 5.4DD409 pKa = 3.78ILKK412 pKa = 10.43VEE414 pKa = 4.08MQNYY418 pKa = 9.97LNGADD423 pKa = 3.81VGDD426 pKa = 4.1VLDD429 pKa = 4.37NAQSQAEE436 pKa = 4.3TQLRR440 pKa = 3.75

Molecular weight: 48.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I6NW54|A0A1I6NW54_9BACI Uncharacterized protein OS=Halolactibacillus miurensis OX=306541 GN=SAMN05421668_10126 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.49KK14 pKa = 8.63VHH16 pKa = 5.57GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.25NGRR28 pKa = 11.84LVLKK32 pKa = 10.3RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.56GRR39 pKa = 11.84KK40 pKa = 8.44VLSAA44 pKa = 4.05

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3011

0

3011

919271

29

2419

305.3

34.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.651 ± 0.041

0.555 ± 0.013

6.214 ± 0.04

7.205 ± 0.049

4.481 ± 0.032

6.277 ± 0.043

2.431 ± 0.026

7.462 ± 0.046

6.368 ± 0.047

9.876 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.798 ± 0.023

4.211 ± 0.035

3.398 ± 0.022

4.131 ± 0.032

4.095 ± 0.032

5.743 ± 0.034

6.188 ± 0.036

7.218 ± 0.039

0.876 ± 0.016

3.821 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski