Amino acid dipepetide frequency for Halolactibacillus miurensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.297AlaAla: 4.297 ± 0.082
0.506AlaCys: 0.506 ± 0.022
3.466AlaAsp: 3.466 ± 0.07
4.258AlaGlu: 4.258 ± 0.075
3.262AlaPhe: 3.262 ± 0.07
4.262AlaGly: 4.262 ± 0.081
1.377AlaHis: 1.377 ± 0.038
5.874AlaIle: 5.874 ± 0.092
4.315AlaLys: 4.315 ± 0.079
7.745AlaLeu: 7.745 ± 0.103
2.121AlaMet: 2.121 ± 0.054
2.571AlaAsn: 2.571 ± 0.053
1.934AlaPro: 1.934 ± 0.05
1.974AlaGln: 1.974 ± 0.048
2.563AlaArg: 2.563 ± 0.055
4.022AlaSer: 4.022 ± 0.07
3.785AlaThr: 3.785 ± 0.064
5.098AlaVal: 5.098 ± 0.081
0.548AlaTrp: 0.548 ± 0.025
2.532AlaTyr: 2.532 ± 0.058
0.0AlaXaa: 0.0 ± 0.0
Cys
0.332CysAla: 0.332 ± 0.019
0.064CysCys: 0.064 ± 0.009
0.387CysAsp: 0.387 ± 0.021
0.337CysGlu: 0.337 ± 0.02
0.272CysPhe: 0.272 ± 0.018
0.488CysGly: 0.488 ± 0.025
0.244CysHis: 0.244 ± 0.013
0.31CysIle: 0.31 ± 0.018
0.237CysLys: 0.237 ± 0.018
0.585CysLeu: 0.585 ± 0.026
0.128CysMet: 0.128 ± 0.011
0.2CysAsn: 0.2 ± 0.016
0.267CysPro: 0.267 ± 0.018
0.284CysGln: 0.284 ± 0.019
0.194CysArg: 0.194 ± 0.016
0.319CysSer: 0.319 ± 0.02
0.251CysThr: 0.251 ± 0.017
0.398CysVal: 0.398 ± 0.019
0.026CysTrp: 0.026 ± 0.006
0.23CysTyr: 0.23 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
3.852AspAla: 3.852 ± 0.081
0.392AspCys: 0.392 ± 0.022
3.879AspAsp: 3.879 ± 0.089
5.199AspGlu: 5.199 ± 0.084
2.759AspPhe: 2.759 ± 0.058
3.763AspGly: 3.763 ± 0.08
1.606AspHis: 1.606 ± 0.048
4.558AspIle: 4.558 ± 0.086
3.294AspLys: 3.294 ± 0.074
6.045AspLeu: 6.045 ± 0.081
1.637AspMet: 1.637 ± 0.045
2.118AspAsn: 2.118 ± 0.051
2.06AspPro: 2.06 ± 0.045
3.298AspGln: 3.298 ± 0.064
2.631AspArg: 2.631 ± 0.056
2.545AspSer: 2.545 ± 0.056
3.525AspThr: 3.525 ± 0.067
5.235AspVal: 5.235 ± 0.078
0.719AspTrp: 0.719 ± 0.031
3.031AspTyr: 3.031 ± 0.052
0.0AspXaa: 0.0 ± 0.0
Glu
6.377GluAla: 6.377 ± 0.098
0.294GluCys: 0.294 ± 0.02
4.253GluAsp: 4.253 ± 0.077
6.075GluGlu: 6.075 ± 0.095
1.887GluPhe: 1.887 ± 0.053
4.091GluGly: 4.091 ± 0.075
1.656GluHis: 1.656 ± 0.05
4.419GluIle: 4.419 ± 0.076
5.49GluLys: 5.49 ± 0.091
6.436GluLeu: 6.436 ± 0.087
2.175GluMet: 2.175 ± 0.048
3.052GluAsn: 3.052 ± 0.061
2.139GluPro: 2.139 ± 0.049
3.663GluGln: 3.663 ± 0.072
3.688GluArg: 3.688 ± 0.062
3.551GluSer: 3.551 ± 0.068
5.177GluThr: 5.177 ± 0.086
5.061GluVal: 5.061 ± 0.088
0.714GluTrp: 0.714 ± 0.031
1.859GluTyr: 1.859 ± 0.045
0.0GluXaa: 0.0 ± 0.0
Phe
2.257PheAla: 2.257 ± 0.054
0.264PheCys: 0.264 ± 0.016
3.056PheAsp: 3.056 ± 0.06
2.822PheGlu: 2.822 ± 0.054
2.291PhePhe: 2.291 ± 0.07
3.108PheGly: 3.108 ± 0.075
1.077PheHis: 1.077 ± 0.036
3.876PheIle: 3.876 ± 0.081
2.97PheLys: 2.97 ± 0.059
4.004PheLeu: 4.004 ± 0.09
1.152PheMet: 1.152 ± 0.037
2.321PheAsn: 2.321 ± 0.054
1.395PhePro: 1.395 ± 0.037
1.448PheGln: 1.448 ± 0.045
1.435PheArg: 1.435 ± 0.034
3.143PheSer: 3.143 ± 0.059
2.831PheThr: 2.831 ± 0.072
3.172PheVal: 3.172 ± 0.059
0.375PheTrp: 0.375 ± 0.022
1.81PheTyr: 1.81 ± 0.045
0.0PheXaa: 0.0 ± 0.0
Gly
4.259GlyAla: 4.259 ± 0.088
0.451GlyCys: 0.451 ± 0.023
3.652GlyAsp: 3.652 ± 0.078
4.407GlyGlu: 4.407 ± 0.073
3.328GlyPhe: 3.328 ± 0.07
4.017GlyGly: 4.017 ± 0.087
1.409GlyHis: 1.409 ± 0.036
4.945GlyIle: 4.945 ± 0.078
3.892GlyLys: 3.892 ± 0.069
6.443GlyLeu: 6.443 ± 0.107
1.931GlyMet: 1.931 ± 0.054
2.195GlyAsn: 2.195 ± 0.053
1.522GlyPro: 1.522 ± 0.042
2.208GlyGln: 2.208 ± 0.047
2.413GlyArg: 2.413 ± 0.059
3.386GlySer: 3.386 ± 0.058
3.658GlyThr: 3.658 ± 0.067
5.128GlyVal: 5.128 ± 0.091
0.635GlyTrp: 0.635 ± 0.028
2.891GlyTyr: 2.891 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
1.613HisAla: 1.613 ± 0.049
0.151HisCys: 0.151 ± 0.014
1.477HisAsp: 1.477 ± 0.047
1.462HisGlu: 1.462 ± 0.046
1.166HisPhe: 1.166 ± 0.036
1.398HisGly: 1.398 ± 0.046
1.012HisHis: 1.012 ± 0.037
1.687HisIle: 1.687 ± 0.046
1.015HisLys: 1.015 ± 0.035
2.544HisLeu: 2.544 ± 0.057
0.654HisMet: 0.654 ± 0.026
0.842HisAsn: 0.842 ± 0.031
1.175HisPro: 1.175 ± 0.035
1.236HisGln: 1.236 ± 0.038
1.015HisArg: 1.015 ± 0.032
1.087HisSer: 1.087 ± 0.034
1.328HisThr: 1.328 ± 0.038
1.919HisVal: 1.919 ± 0.048
0.258HisTrp: 0.258 ± 0.015
1.266HisTyr: 1.266 ± 0.038
0.0HisXaa: 0.0 ± 0.0
Ile
5.144IleAla: 5.144 ± 0.079
0.387IleCys: 0.387 ± 0.022
5.609IleAsp: 5.609 ± 0.075
6.016IleGlu: 6.016 ± 0.086
3.156IlePhe: 3.156 ± 0.081
5.371IleGly: 5.371 ± 0.092
1.833IleHis: 1.833 ± 0.046
5.604IleIle: 5.604 ± 0.106
4.869IleLys: 4.869 ± 0.078
6.481IleLeu: 6.481 ± 0.106
1.568IleMet: 1.568 ± 0.042
3.646IleAsn: 3.646 ± 0.069
2.808IlePro: 2.808 ± 0.068
2.786IleGln: 2.786 ± 0.058
2.635IleArg: 2.635 ± 0.058
4.307IleSer: 4.307 ± 0.073
4.207IleThr: 4.207 ± 0.068
5.173IleVal: 5.173 ± 0.081
0.494IleTrp: 0.494 ± 0.025
2.525IleTyr: 2.525 ± 0.055
0.0IleXaa: 0.0 ± 0.0
Lys
4.58LysAla: 4.58 ± 0.08
0.247LysCys: 0.247 ± 0.016
4.332LysAsp: 4.332 ± 0.08
5.893LysGlu: 5.893 ± 0.089
1.504LysPhe: 1.504 ± 0.045
3.684LysGly: 3.684 ± 0.06
1.725LysHis: 1.725 ± 0.045
3.824LysIle: 3.824 ± 0.059
5.294LysLys: 5.294 ± 0.091
4.913LysLeu: 4.913 ± 0.079
1.86LysMet: 1.86 ± 0.05
2.771LysAsn: 2.771 ± 0.066
1.984LysPro: 1.984 ± 0.046
4.005LysGln: 4.005 ± 0.078
3.554LysArg: 3.554 ± 0.064
3.058LysSer: 3.058 ± 0.059
3.836LysThr: 3.836 ± 0.075
4.24LysVal: 4.24 ± 0.077
0.528LysTrp: 0.528 ± 0.026
1.845LysTyr: 1.845 ± 0.05
0.0LysXaa: 0.0 ± 0.0
Leu
6.767LeuAla: 6.767 ± 0.096
0.425LeuCys: 0.425 ± 0.026
5.983LeuAsp: 5.983 ± 0.083
6.251LeuGlu: 6.251 ± 0.094
4.731LeuPhe: 4.731 ± 0.098
5.88LeuGly: 5.88 ± 0.097
1.985LeuHis: 1.985 ± 0.046
7.595LeuIle: 7.595 ± 0.11
6.659LeuLys: 6.659 ± 0.097
9.603LeuLeu: 9.603 ± 0.145
2.792LeuMet: 2.792 ± 0.059
4.714LeuAsn: 4.714 ± 0.089
3.887LeuPro: 3.887 ± 0.074
3.055LeuGln: 3.055 ± 0.059
3.584LeuArg: 3.584 ± 0.066
7.278LeuSer: 7.278 ± 0.097
7.165LeuThr: 7.165 ± 0.092
6.414LeuVal: 6.414 ± 0.08
0.657LeuTrp: 0.657 ± 0.029
3.333LeuTyr: 3.333 ± 0.065
0.0LeuXaa: 0.0 ± 0.0
Met
2.01MetAla: 2.01 ± 0.059
0.129MetCys: 0.129 ± 0.011
1.494MetAsp: 1.494 ± 0.036
1.537MetGlu: 1.537 ± 0.043
1.089MetPhe: 1.089 ± 0.036
1.581MetGly: 1.581 ± 0.041
0.565MetHis: 0.565 ± 0.024
2.351MetIle: 2.351 ± 0.048
2.169MetLys: 2.169 ± 0.047
2.594MetLeu: 2.594 ± 0.055
1.0MetMet: 1.0 ± 0.038
1.412MetAsn: 1.412 ± 0.04
0.979MetPro: 0.979 ± 0.036
0.913MetGln: 0.913 ± 0.033
1.274MetArg: 1.274 ± 0.039
1.761MetSer: 1.761 ± 0.045
2.219MetThr: 2.219 ± 0.046
1.895MetVal: 1.895 ± 0.047
0.176MetTrp: 0.176 ± 0.014
0.835MetTyr: 0.835 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
2.455AsnAla: 2.455 ± 0.062
0.219AsnCys: 0.219 ± 0.014
2.876AsnAsp: 2.876 ± 0.057
3.195AsnGlu: 3.195 ± 0.068
1.632AsnPhe: 1.632 ± 0.049
2.797AsnGly: 2.797 ± 0.058
1.221AsnHis: 1.221 ± 0.034
3.03AsnIle: 3.03 ± 0.063
2.813AsnLys: 2.813 ± 0.061
3.36AsnLeu: 3.36 ± 0.066
1.203AsnMet: 1.203 ± 0.033
2.034AsnAsn: 2.034 ± 0.055
1.748AsnPro: 1.748 ± 0.043
2.532AsnGln: 2.532 ± 0.056
2.079AsnArg: 2.079 ± 0.051
1.78AsnSer: 1.78 ± 0.047
2.148AsnThr: 2.148 ± 0.052
2.945AsnVal: 2.945 ± 0.058
0.443AsnTrp: 0.443 ± 0.024
1.606AsnTyr: 1.606 ± 0.044
0.0AsnXaa: 0.0 ± 0.0
Pro
1.985ProAla: 1.985 ± 0.047
0.19ProCys: 0.19 ± 0.016
2.145ProAsp: 2.145 ± 0.051
2.836ProGlu: 2.836 ± 0.053
1.897ProPhe: 1.897 ± 0.046
2.026ProGly: 2.026 ± 0.056
0.862ProHis: 0.862 ± 0.029
2.712ProIle: 2.712 ± 0.056
1.955ProLys: 1.955 ± 0.051
3.498ProLeu: 3.498 ± 0.067
0.895ProMet: 0.895 ± 0.03
1.518ProAsn: 1.518 ± 0.042
0.783ProPro: 0.783 ± 0.031
0.938ProGln: 0.938 ± 0.032
1.006ProArg: 1.006 ± 0.033
2.018ProSer: 2.018 ± 0.051
2.118ProThr: 2.118 ± 0.045
2.766ProVal: 2.766 ± 0.056
0.315ProTrp: 0.315 ± 0.02
1.515ProTyr: 1.515 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
3.707GlnAla: 3.707 ± 0.074
0.216GlnCys: 0.216 ± 0.017
2.027GlnAsp: 2.027 ± 0.046
2.477GlnGlu: 2.477 ± 0.054
1.77GlnPhe: 1.77 ± 0.045
2.17GlnGly: 2.17 ± 0.052
1.004GlnHis: 1.004 ± 0.038
2.489GlnIle: 2.489 ± 0.055
2.35GlnLys: 2.35 ± 0.057
4.932GlnLeu: 4.932 ± 0.091
1.279GlnMet: 1.279 ± 0.034
1.354GlnAsn: 1.354 ± 0.039
1.446GlnPro: 1.446 ± 0.041
2.254GlnGln: 2.254 ± 0.065
1.611GlnArg: 1.611 ± 0.045
2.735GlnSer: 2.735 ± 0.052
2.895GlnThr: 2.895 ± 0.059
2.672GlnVal: 2.672 ± 0.056
0.429GlnTrp: 0.429 ± 0.024
1.492GlnTyr: 1.492 ± 0.045
0.0GlnXaa: 0.0 ± 0.0
Arg
2.493ArgAla: 2.493 ± 0.059
0.176ArgCys: 0.176 ± 0.015
2.377ArgAsp: 2.377 ± 0.044
3.185ArgGlu: 3.185 ± 0.062
2.132ArgPhe: 2.132 ± 0.049
2.313ArgGly: 2.313 ± 0.048
1.05ArgHis: 1.05 ± 0.035
2.752ArgIle: 2.752 ± 0.064
2.584ArgLys: 2.584 ± 0.056
4.689ArgLeu: 4.689 ± 0.074
1.234ArgMet: 1.234 ± 0.036
1.434ArgAsn: 1.434 ± 0.038
1.334ArgPro: 1.334 ± 0.038
1.949ArgGln: 1.949 ± 0.05
1.825ArgArg: 1.825 ± 0.05
2.021ArgSer: 2.021 ± 0.045
2.125ArgThr: 2.125 ± 0.056
2.878ArgVal: 2.878 ± 0.058
0.369ArgTrp: 0.369 ± 0.019
2.031ArgTyr: 2.031 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
3.217SerAla: 3.217 ± 0.062
0.286SerCys: 0.286 ± 0.019
3.674SerAsp: 3.674 ± 0.076
3.966SerGlu: 3.966 ± 0.075
3.113SerPhe: 3.113 ± 0.068
4.257SerGly: 4.257 ± 0.075
1.422SerHis: 1.422 ± 0.038
4.463SerIle: 4.463 ± 0.07
3.234SerLys: 3.234 ± 0.06
5.986SerLeu: 5.986 ± 0.093
1.66SerMet: 1.66 ± 0.042
2.301SerAsn: 2.301 ± 0.054
1.854SerPro: 1.854 ± 0.041
2.151SerGln: 2.151 ± 0.044
2.314SerArg: 2.314 ± 0.05
3.295SerSer: 3.295 ± 0.067
3.172SerThr: 3.172 ± 0.064
4.135SerVal: 4.135 ± 0.062
0.533SerTrp: 0.533 ± 0.026
2.401SerTyr: 2.401 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
3.893ThrAla: 3.893 ± 0.073
0.387ThrCys: 0.387 ± 0.022
4.001ThrAsp: 4.001 ± 0.085
4.114ThrGlu: 4.114 ± 0.083
3.083ThrPhe: 3.083 ± 0.05
4.169ThrGly: 4.169 ± 0.067
1.515ThrHis: 1.515 ± 0.04
4.966ThrIle: 4.966 ± 0.086
3.506ThrLys: 3.506 ± 0.067
6.798ThrLeu: 6.798 ± 0.101
1.426ThrMet: 1.426 ± 0.036
2.528ThrAsn: 2.528 ± 0.05
2.396ThrPro: 2.396 ± 0.05
1.845ThrGln: 1.845 ± 0.045
2.222ThrArg: 2.222 ± 0.054
3.674ThrSer: 3.674 ± 0.062
3.838ThrThr: 3.838 ± 0.087
4.629ThrVal: 4.629 ± 0.088
0.525ThrTrp: 0.525 ± 0.026
2.369ThrTyr: 2.369 ± 0.064
0.0ThrXaa: 0.0 ± 0.0
Val
4.624ValAla: 4.624 ± 0.077
0.476ValCys: 0.476 ± 0.024
4.53ValAsp: 4.53 ± 0.07
5.016ValGlu: 5.016 ± 0.078
3.327ValPhe: 3.327 ± 0.068
4.512ValGly: 4.512 ± 0.076
1.312ValHis: 1.312 ± 0.036
6.057ValIle: 6.057 ± 0.081
4.72ValLys: 4.72 ± 0.072
6.914ValLeu: 6.914 ± 0.089
2.084ValMet: 2.084 ± 0.048
3.15ValAsn: 3.15 ± 0.055
2.444ValPro: 2.444 ± 0.048
1.992ValGln: 1.992 ± 0.049
2.656ValArg: 2.656 ± 0.054
4.864ValSer: 4.864 ± 0.073
5.013ValThr: 5.013 ± 0.085
5.394ValVal: 5.394 ± 0.088
0.554ValTrp: 0.554 ± 0.023
2.537ValTyr: 2.537 ± 0.056
0.0ValXaa: 0.0 ± 0.0
Trp
0.521TrpAla: 0.521 ± 0.028
0.066TrpCys: 0.066 ± 0.008
0.504TrpAsp: 0.504 ± 0.023
0.504TrpGlu: 0.504 ± 0.026
0.52TrpPhe: 0.52 ± 0.025
0.516TrpGly: 0.516 ± 0.026
0.203TrpHis: 0.203 ± 0.016
0.618TrpIle: 0.618 ± 0.024
0.425TrpLys: 0.425 ± 0.021
1.069TrpLeu: 1.069 ± 0.038
0.285TrpMet: 0.285 ± 0.015
0.362TrpAsn: 0.362 ± 0.022
0.285TrpPro: 0.285 ± 0.018
0.473TrpGln: 0.473 ± 0.022
0.398TrpArg: 0.398 ± 0.022
0.525TrpSer: 0.525 ± 0.023
0.46TrpThr: 0.46 ± 0.021
0.562TrpVal: 0.562 ± 0.022
0.101TrpTrp: 0.101 ± 0.01
0.366TrpTyr: 0.366 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.127TyrAla: 2.127 ± 0.043
0.224TyrCys: 0.224 ± 0.017
2.425TyrAsp: 2.425 ± 0.052
2.506TyrGlu: 2.506 ± 0.052
1.887TyrPhe: 1.887 ± 0.045
2.37TyrGly: 2.37 ± 0.046
1.229TyrHis: 1.229 ± 0.04
2.487TyrIle: 2.487 ± 0.051
1.885TyrLys: 1.885 ± 0.048
4.116TyrLeu: 4.116 ± 0.08
0.897TyrMet: 0.897 ± 0.031
1.591TyrAsn: 1.591 ± 0.044
1.54TyrPro: 1.54 ± 0.044
2.305TyrGln: 2.305 ± 0.051
1.853TyrArg: 1.853 ± 0.049
2.066TyrSer: 2.066 ± 0.048
2.135TyrThr: 2.135 ± 0.052
2.464TyrVal: 2.464 ± 0.057
0.366TyrTrp: 0.366 ± 0.02
1.733TyrTyr: 1.733 ± 0.049
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3011 proteins (919271 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski