Bacillus phage PfIS075

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cecivirus; unclassified Cecivirus

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1P858|A0A1B1P858_9CAUD Uncharacterized protein OS=Bacillus phage PfIS075 OX=1868600 PE=4 SV=1
MM1 pKa = 7.65EE2 pKa = 4.74LTLEE6 pKa = 4.3GLEE9 pKa = 4.0QCFNEE14 pKa = 4.41AASEE18 pKa = 4.03EE19 pKa = 4.25ANYY22 pKa = 10.53VAVQIEE28 pKa = 4.37MDD30 pKa = 4.39GFPSDD35 pKa = 3.82EE36 pKa = 4.09VIINDD41 pKa = 3.32KK42 pKa = 11.08HH43 pKa = 7.35NIVSKK48 pKa = 11.04LEE50 pKa = 4.15YY51 pKa = 10.22YY52 pKa = 10.53KK53 pKa = 9.84KK54 pKa = 9.26TYY56 pKa = 10.83NEE58 pKa = 4.25DD59 pKa = 3.34LEE61 pKa = 4.29HH62 pKa = 7.21RR63 pKa = 11.84YY64 pKa = 9.25AAGIRR69 pKa = 11.84IVGYY73 pKa = 9.93AYY75 pKa = 9.92GYY77 pKa = 10.2SFSGVQRR84 pKa = 11.84EE85 pKa = 4.03LGLVIEE91 pKa = 4.73

Molecular weight: 10.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1P8E4|A0A1B1P8E4_9CAUD Uncharacterized protein OS=Bacillus phage PfIS075 OX=1868600 PE=4 SV=1
MM1 pKa = 7.2EE2 pKa = 5.51RR3 pKa = 11.84MRR5 pKa = 11.84HH6 pKa = 3.88TRR8 pKa = 11.84NRR10 pKa = 11.84QMTKK14 pKa = 9.4IGEE17 pKa = 4.17EE18 pKa = 3.7NFMGMKK24 pKa = 9.74NIKK27 pKa = 9.73ISTIRR32 pKa = 11.84KK33 pKa = 9.08FDD35 pKa = 3.56GEE37 pKa = 4.45FNNRR41 pKa = 11.84LDD43 pKa = 3.56PTFRR47 pKa = 11.84WHH49 pKa = 7.54RR50 pKa = 11.84DD51 pKa = 3.27YY52 pKa = 11.54HH53 pKa = 6.99GMNIVSFNRR62 pKa = 11.84KK63 pKa = 9.3GEE65 pKa = 4.0AFKK68 pKa = 11.02TNVIKK73 pKa = 10.88VRR75 pKa = 11.84NN76 pKa = 3.71

Molecular weight: 9.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

13938

39

1310

172.1

19.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.292 ± 0.283

0.689 ± 0.109

5.682 ± 0.258

8.143 ± 0.508

3.781 ± 0.166

6.077 ± 0.337

1.456 ± 0.11

7.426 ± 0.343

8.717 ± 0.447

8.093 ± 0.295

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.171 ± 0.35

5.568 ± 0.243

3.078 ± 0.204

3.968 ± 0.256

4.549 ± 0.305

5.797 ± 0.285

5.854 ± 0.284

6.421 ± 0.244

1.169 ± 0.099

4.068 ± 0.282
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski