Amino acid dipepetide frequency for Bacillus phage PfIS075

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.449AlaAla: 4.449 ± 0.607
0.574AlaCys: 0.574 ± 0.175
2.798AlaAsp: 2.798 ± 0.418
5.597AlaGlu: 5.597 ± 0.527
3.014AlaPhe: 3.014 ± 0.396
3.157AlaGly: 3.157 ± 0.476
0.359AlaHis: 0.359 ± 0.146
4.879AlaIle: 4.879 ± 0.466
5.525AlaLys: 5.525 ± 0.536
6.027AlaLeu: 6.027 ± 0.766
2.511AlaMet: 2.511 ± 0.591
3.731AlaAsn: 3.731 ± 0.478
1.794AlaPro: 1.794 ± 0.377
2.727AlaGln: 2.727 ± 0.499
2.44AlaArg: 2.44 ± 0.597
3.229AlaSer: 3.229 ± 0.441
3.301AlaThr: 3.301 ± 0.659
4.162AlaVal: 4.162 ± 0.543
0.502AlaTrp: 0.502 ± 0.176
2.153AlaTyr: 2.153 ± 0.54
0.0AlaXaa: 0.0 ± 0.0
Cys
0.359CysAla: 0.359 ± 0.167
0.072CysCys: 0.072 ± 0.074
0.574CysAsp: 0.574 ± 0.238
0.861CysGlu: 0.861 ± 0.292
0.574CysPhe: 0.574 ± 0.213
0.215CysGly: 0.215 ± 0.144
0.144CysHis: 0.144 ± 0.116
0.287CysIle: 0.287 ± 0.128
0.287CysLys: 0.287 ± 0.174
0.215CysLeu: 0.215 ± 0.114
0.0CysMet: 0.0 ± 0.0
0.502CysAsn: 0.502 ± 0.222
0.502CysPro: 0.502 ± 0.234
0.144CysGln: 0.144 ± 0.096
0.215CysArg: 0.215 ± 0.12
0.431CysSer: 0.431 ± 0.174
0.502CysThr: 0.502 ± 0.224
0.646CysVal: 0.646 ± 0.226
0.0CysTrp: 0.0 ± 0.0
0.359CysTyr: 0.359 ± 0.191
0.0CysXaa: 0.0 ± 0.0
Asp
3.157AspAla: 3.157 ± 0.397
0.215AspCys: 0.215 ± 0.116
3.372AspAsp: 3.372 ± 0.611
5.023AspGlu: 5.023 ± 0.796
2.511AspPhe: 2.511 ± 0.433
3.588AspGly: 3.588 ± 0.594
1.148AspHis: 1.148 ± 0.308
4.736AspIle: 4.736 ± 0.572
4.305AspLys: 4.305 ± 0.587
5.238AspLeu: 5.238 ± 0.791
2.296AspMet: 2.296 ± 0.43
2.583AspAsn: 2.583 ± 0.368
1.22AspPro: 1.22 ± 0.325
1.794AspGln: 1.794 ± 0.324
2.224AspArg: 2.224 ± 0.455
2.87AspSer: 2.87 ± 0.408
2.511AspThr: 2.511 ± 0.456
4.377AspVal: 4.377 ± 0.675
0.718AspTrp: 0.718 ± 0.219
2.942AspTyr: 2.942 ± 0.492
0.0AspXaa: 0.0 ± 0.0
Glu
6.745GluAla: 6.745 ± 0.673
1.148GluCys: 1.148 ± 0.325
3.516GluAsp: 3.516 ± 0.528
7.677GluGlu: 7.677 ± 1.182
2.87GluPhe: 2.87 ± 0.421
4.592GluGly: 4.592 ± 0.498
1.22GluHis: 1.22 ± 0.329
6.242GluIle: 6.242 ± 0.73
7.032GluLys: 7.032 ± 0.878
7.247GluLeu: 7.247 ± 0.821
2.44GluMet: 2.44 ± 0.532
4.305GluAsn: 4.305 ± 0.582
2.224GluPro: 2.224 ± 0.613
3.085GluGln: 3.085 ± 0.555
3.372GluArg: 3.372 ± 0.531
4.52GluSer: 4.52 ± 0.476
3.372GluThr: 3.372 ± 0.385
5.166GluVal: 5.166 ± 0.66
1.579GluTrp: 1.579 ± 0.296
3.014GluTyr: 3.014 ± 0.598
0.0GluXaa: 0.0 ± 0.0
Phe
1.866PheAla: 1.866 ± 0.256
0.287PheCys: 0.287 ± 0.135
2.727PheAsp: 2.727 ± 0.52
2.727PheGlu: 2.727 ± 0.525
1.076PhePhe: 1.076 ± 0.248
2.081PheGly: 2.081 ± 0.359
0.431PheHis: 0.431 ± 0.213
3.229PheIle: 3.229 ± 0.512
2.44PheLys: 2.44 ± 0.357
2.583PheLeu: 2.583 ± 0.352
0.933PheMet: 0.933 ± 0.248
2.296PheAsn: 2.296 ± 0.379
1.435PhePro: 1.435 ± 0.327
1.435PheGln: 1.435 ± 0.311
2.153PheArg: 2.153 ± 0.356
2.942PheSer: 2.942 ± 0.392
2.798PheThr: 2.798 ± 0.348
2.153PheVal: 2.153 ± 0.415
0.359PheTrp: 0.359 ± 0.164
1.866PheTyr: 1.866 ± 0.408
0.0PheXaa: 0.0 ± 0.0
Gly
2.727GlyAla: 2.727 ± 0.447
0.431GlyCys: 0.431 ± 0.19
3.372GlyAsp: 3.372 ± 0.564
4.162GlyGlu: 4.162 ± 0.545
3.085GlyPhe: 3.085 ± 0.475
4.018GlyGly: 4.018 ± 0.522
0.789GlyHis: 0.789 ± 0.205
6.027GlyIle: 6.027 ± 0.891
5.74GlyLys: 5.74 ± 0.593
5.166GlyLeu: 5.166 ± 0.865
3.014GlyMet: 3.014 ± 0.534
3.085GlyAsn: 3.085 ± 0.639
0.718GlyPro: 0.718 ± 0.237
2.009GlyGln: 2.009 ± 0.425
2.368GlyArg: 2.368 ± 0.38
2.655GlySer: 2.655 ± 0.348
3.444GlyThr: 3.444 ± 0.589
3.875GlyVal: 3.875 ± 0.405
0.646GlyTrp: 0.646 ± 0.234
3.444GlyTyr: 3.444 ± 0.483
0.0GlyXaa: 0.0 ± 0.0
His
0.646HisAla: 0.646 ± 0.209
0.072HisCys: 0.072 ± 0.074
0.861HisAsp: 0.861 ± 0.213
1.579HisGlu: 1.579 ± 0.354
0.574HisPhe: 0.574 ± 0.201
0.933HisGly: 0.933 ± 0.204
0.359HisHis: 0.359 ± 0.178
1.292HisIle: 1.292 ± 0.317
0.933HisLys: 0.933 ± 0.281
1.363HisLeu: 1.363 ± 0.329
0.215HisMet: 0.215 ± 0.135
0.861HisAsn: 0.861 ± 0.243
0.574HisPro: 0.574 ± 0.156
0.431HisGln: 0.431 ± 0.168
0.933HisArg: 0.933 ± 0.285
0.933HisSer: 0.933 ± 0.24
0.646HisThr: 0.646 ± 0.279
0.718HisVal: 0.718 ± 0.27
0.144HisTrp: 0.144 ± 0.088
0.502HisTyr: 0.502 ± 0.21
0.0HisXaa: 0.0 ± 0.0
Ile
6.027IleAla: 6.027 ± 0.551
0.215IleCys: 0.215 ± 0.144
5.381IleAsp: 5.381 ± 0.61
6.96IleGlu: 6.96 ± 0.699
1.363IlePhe: 1.363 ± 0.355
3.875IleGly: 3.875 ± 0.768
1.363IleHis: 1.363 ± 0.346
5.023IleIle: 5.023 ± 0.853
6.529IleLys: 6.529 ± 0.719
4.951IleLeu: 4.951 ± 0.854
1.65IleMet: 1.65 ± 0.304
5.094IleAsn: 5.094 ± 0.74
2.583IlePro: 2.583 ± 0.472
3.085IleGln: 3.085 ± 0.707
3.946IleArg: 3.946 ± 0.384
3.803IleSer: 3.803 ± 0.729
4.951IleThr: 4.951 ± 0.628
4.377IleVal: 4.377 ± 0.702
0.861IleTrp: 0.861 ± 0.226
2.224IleTyr: 2.224 ± 0.563
0.0IleXaa: 0.0 ± 0.0
Lys
4.664LysAla: 4.664 ± 0.588
0.431LysCys: 0.431 ± 0.172
5.381LysAsp: 5.381 ± 0.83
7.964LysGlu: 7.964 ± 1.23
2.368LysPhe: 2.368 ± 0.399
4.951LysGly: 4.951 ± 0.77
1.435LysHis: 1.435 ± 0.347
4.305LysIle: 4.305 ± 0.517
8.682LysLys: 8.682 ± 1.439
7.677LysLeu: 7.677 ± 0.782
2.511LysMet: 2.511 ± 0.371
3.659LysAsn: 3.659 ± 0.497
2.368LysPro: 2.368 ± 0.427
4.233LysGln: 4.233 ± 0.537
4.736LysArg: 4.736 ± 0.61
5.381LysSer: 5.381 ± 0.529
5.668LysThr: 5.668 ± 0.529
5.31LysVal: 5.31 ± 0.579
1.435LysTrp: 1.435 ± 0.309
4.018LysTyr: 4.018 ± 0.614
0.0LysXaa: 0.0 ± 0.0
Leu
5.023LeuAla: 5.023 ± 0.582
0.431LeuCys: 0.431 ± 0.186
4.736LeuAsp: 4.736 ± 0.551
6.171LeuGlu: 6.171 ± 0.794
2.942LeuPhe: 2.942 ± 0.49
5.238LeuGly: 5.238 ± 0.756
1.005LeuHis: 1.005 ± 0.246
5.453LeuIle: 5.453 ± 0.529
7.39LeuLys: 7.39 ± 0.808
5.955LeuLeu: 5.955 ± 0.806
2.511LeuMet: 2.511 ± 0.465
4.592LeuAsn: 4.592 ± 0.734
3.085LeuPro: 3.085 ± 0.871
3.372LeuGln: 3.372 ± 0.516
4.377LeuArg: 4.377 ± 0.525
5.166LeuSer: 5.166 ± 0.663
4.664LeuThr: 4.664 ± 0.742
4.233LeuVal: 4.233 ± 0.571
0.574LeuTrp: 0.574 ± 0.191
4.018LeuTyr: 4.018 ± 0.717
0.0LeuXaa: 0.0 ± 0.0
Met
2.296MetAla: 2.296 ± 0.393
0.287MetCys: 0.287 ± 0.159
1.435MetAsp: 1.435 ± 0.332
3.085MetGlu: 3.085 ± 0.571
0.646MetPhe: 0.646 ± 0.206
2.153MetGly: 2.153 ± 0.931
0.215MetHis: 0.215 ± 0.107
1.937MetIle: 1.937 ± 0.353
3.301MetLys: 3.301 ± 0.348
2.44MetLeu: 2.44 ± 0.427
1.435MetMet: 1.435 ± 0.496
2.44MetAsn: 2.44 ± 0.454
0.574MetPro: 0.574 ± 0.241
1.507MetGln: 1.507 ± 0.323
1.507MetArg: 1.507 ± 0.384
1.866MetSer: 1.866 ± 0.437
1.794MetThr: 1.794 ± 0.406
1.292MetVal: 1.292 ± 0.386
0.287MetTrp: 0.287 ± 0.13
1.22MetTyr: 1.22 ± 0.315
0.0MetXaa: 0.0 ± 0.0
Asn
2.44AsnAla: 2.44 ± 0.514
0.502AsnCys: 0.502 ± 0.233
3.229AsnAsp: 3.229 ± 0.57
3.803AsnGlu: 3.803 ± 0.589
2.153AsnPhe: 2.153 ± 0.304
5.094AsnGly: 5.094 ± 0.675
0.718AsnHis: 0.718 ± 0.25
4.162AsnIle: 4.162 ± 0.567
4.951AsnLys: 4.951 ± 0.668
4.807AsnLeu: 4.807 ± 0.615
1.937AsnMet: 1.937 ± 0.54
3.301AsnAsn: 3.301 ± 0.406
2.44AsnPro: 2.44 ± 0.43
2.296AsnGln: 2.296 ± 0.362
1.794AsnArg: 1.794 ± 0.429
3.372AsnSer: 3.372 ± 0.733
2.87AsnThr: 2.87 ± 0.404
3.803AsnVal: 3.803 ± 0.414
0.431AsnTrp: 0.431 ± 0.18
1.579AsnTyr: 1.579 ± 0.313
0.0AsnXaa: 0.0 ± 0.0
Pro
1.579ProAla: 1.579 ± 0.317
0.287ProCys: 0.287 ± 0.149
1.722ProAsp: 1.722 ± 0.292
2.296ProGlu: 2.296 ± 0.444
1.292ProPhe: 1.292 ± 0.293
1.794ProGly: 1.794 ± 0.36
0.287ProHis: 0.287 ± 0.144
1.65ProIle: 1.65 ± 0.351
2.081ProLys: 2.081 ± 0.427
2.081ProLeu: 2.081 ± 0.357
0.718ProMet: 0.718 ± 0.218
1.292ProAsn: 1.292 ± 0.301
1.148ProPro: 1.148 ± 0.335
1.65ProGln: 1.65 ± 0.354
1.076ProArg: 1.076 ± 0.231
2.798ProSer: 2.798 ± 0.417
2.655ProThr: 2.655 ± 0.479
2.655ProVal: 2.655 ± 0.531
0.215ProTrp: 0.215 ± 0.115
1.507ProTyr: 1.507 ± 0.355
0.0ProXaa: 0.0 ± 0.0
Gln
1.937GlnAla: 1.937 ± 0.431
0.215GlnCys: 0.215 ± 0.133
1.866GlnAsp: 1.866 ± 0.292
2.583GlnGlu: 2.583 ± 0.485
0.933GlnPhe: 0.933 ± 0.27
2.44GlnGly: 2.44 ± 0.396
0.359GlnHis: 0.359 ± 0.178
3.803GlnIle: 3.803 ± 0.512
3.301GlnLys: 3.301 ± 0.417
3.014GlnLeu: 3.014 ± 0.546
1.435GlnMet: 1.435 ± 0.299
2.009GlnAsn: 2.009 ± 0.521
1.363GlnPro: 1.363 ± 0.292
2.44GlnGln: 2.44 ± 0.629
2.583GlnArg: 2.583 ± 0.435
1.866GlnSer: 1.866 ± 0.289
2.44GlnThr: 2.44 ± 0.337
2.511GlnVal: 2.511 ± 0.469
0.718GlnTrp: 0.718 ± 0.235
1.866GlnTyr: 1.866 ± 0.295
0.0GlnXaa: 0.0 ± 0.0
Arg
2.655ArgAla: 2.655 ± 0.448
0.359ArgCys: 0.359 ± 0.2
2.798ArgAsp: 2.798 ± 0.488
3.731ArgGlu: 3.731 ± 0.563
1.937ArgPhe: 1.937 ± 0.383
2.224ArgGly: 2.224 ± 0.426
0.933ArgHis: 0.933 ± 0.267
4.09ArgIle: 4.09 ± 0.526
4.807ArgLys: 4.807 ± 0.805
3.659ArgLeu: 3.659 ± 0.481
1.435ArgMet: 1.435 ± 0.271
2.368ArgAsn: 2.368 ± 0.415
1.076ArgPro: 1.076 ± 0.281
1.507ArgGln: 1.507 ± 0.405
2.368ArgArg: 2.368 ± 0.441
2.153ArgSer: 2.153 ± 0.356
2.655ArgThr: 2.655 ± 0.468
2.727ArgVal: 2.727 ± 0.472
0.431ArgTrp: 0.431 ± 0.152
1.579ArgTyr: 1.579 ± 0.274
0.0ArgXaa: 0.0 ± 0.0
Ser
3.301SerAla: 3.301 ± 0.543
0.215SerCys: 0.215 ± 0.126
2.798SerAsp: 2.798 ± 0.509
4.09SerGlu: 4.09 ± 0.64
3.085SerPhe: 3.085 ± 0.488
3.444SerGly: 3.444 ± 0.504
0.933SerHis: 0.933 ± 0.26
3.803SerIle: 3.803 ± 0.54
5.812SerLys: 5.812 ± 0.581
4.592SerLeu: 4.592 ± 0.5
1.722SerMet: 1.722 ± 0.36
2.942SerAsn: 2.942 ± 0.543
1.579SerPro: 1.579 ± 0.332
2.727SerGln: 2.727 ± 0.418
2.44SerArg: 2.44 ± 0.477
3.731SerSer: 3.731 ± 0.836
3.229SerThr: 3.229 ± 0.509
4.592SerVal: 4.592 ± 0.69
0.718SerTrp: 0.718 ± 0.21
2.224SerTyr: 2.224 ± 0.487
0.0SerXaa: 0.0 ± 0.0
Thr
5.166ThrAla: 5.166 ± 0.845
0.359ThrCys: 0.359 ± 0.197
2.511ThrAsp: 2.511 ± 0.359
2.727ThrGlu: 2.727 ± 0.467
2.942ThrPhe: 2.942 ± 0.427
3.946ThrGly: 3.946 ± 0.551
1.148ThrHis: 1.148 ± 0.261
4.52ThrIle: 4.52 ± 0.914
4.377ThrLys: 4.377 ± 0.638
5.525ThrLeu: 5.525 ± 0.919
1.363ThrMet: 1.363 ± 0.355
4.09ThrAsn: 4.09 ± 0.584
3.372ThrPro: 3.372 ± 0.555
1.65ThrGln: 1.65 ± 0.377
1.937ThrArg: 1.937 ± 0.41
3.229ThrSer: 3.229 ± 0.578
3.157ThrThr: 3.157 ± 0.48
4.018ThrVal: 4.018 ± 0.498
0.646ThrTrp: 0.646 ± 0.194
1.866ThrTyr: 1.866 ± 0.326
0.0ThrXaa: 0.0 ± 0.0
Val
4.377ValAla: 4.377 ± 0.704
0.431ValCys: 0.431 ± 0.185
4.449ValAsp: 4.449 ± 0.622
5.31ValGlu: 5.31 ± 0.666
1.866ValPhe: 1.866 ± 0.362
4.162ValGly: 4.162 ± 0.624
0.933ValHis: 0.933 ± 0.342
4.807ValIle: 4.807 ± 0.53
5.812ValLys: 5.812 ± 0.655
3.731ValLeu: 3.731 ± 0.512
2.009ValMet: 2.009 ± 0.464
3.229ValAsn: 3.229 ± 0.477
2.224ValPro: 2.224 ± 0.367
2.081ValGln: 2.081 ± 0.424
2.511ValArg: 2.511 ± 0.464
4.233ValSer: 4.233 ± 0.478
4.736ValThr: 4.736 ± 0.793
4.592ValVal: 4.592 ± 0.567
0.861ValTrp: 0.861 ± 0.237
1.866ValTyr: 1.866 ± 0.427
0.0ValXaa: 0.0 ± 0.0
Trp
0.718TrpAla: 0.718 ± 0.222
0.072TrpCys: 0.072 ± 0.076
0.933TrpAsp: 0.933 ± 0.263
1.579TrpGlu: 1.579 ± 0.345
0.646TrpPhe: 0.646 ± 0.186
0.431TrpGly: 0.431 ± 0.196
0.431TrpHis: 0.431 ± 0.201
0.933TrpIle: 0.933 ± 0.253
0.789TrpLys: 0.789 ± 0.218
0.933TrpLeu: 0.933 ± 0.266
0.215TrpMet: 0.215 ± 0.122
0.933TrpAsn: 0.933 ± 0.257
0.0TrpPro: 0.0 ± 0.0
0.144TrpGln: 0.144 ± 0.1
0.646TrpArg: 0.646 ± 0.203
0.574TrpSer: 0.574 ± 0.239
0.718TrpThr: 0.718 ± 0.197
0.574TrpVal: 0.574 ± 0.231
0.144TrpTrp: 0.144 ± 0.105
0.287TrpTyr: 0.287 ± 0.154
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.798TyrAla: 2.798 ± 0.465
0.287TyrCys: 0.287 ± 0.191
2.368TyrAsp: 2.368 ± 0.335
3.516TyrGlu: 3.516 ± 0.676
1.937TyrPhe: 1.937 ± 0.427
2.44TyrGly: 2.44 ± 0.353
0.359TyrHis: 0.359 ± 0.167
3.085TyrIle: 3.085 ± 0.416
3.085TyrLys: 3.085 ± 0.433
3.731TyrLeu: 3.731 ± 0.51
1.292TyrMet: 1.292 ± 0.301
2.368TyrAsn: 2.368 ± 0.402
0.502TyrPro: 0.502 ± 0.216
1.363TyrGln: 1.363 ± 0.395
1.866TyrArg: 1.866 ± 0.341
2.224TyrSer: 2.224 ± 0.442
2.44TyrThr: 2.44 ± 0.499
2.44TyrVal: 2.44 ± 0.485
0.431TyrTrp: 0.431 ± 0.155
2.153TyrTyr: 2.153 ± 0.497
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 81 proteins (13938 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski