Cryphonectria nitschkei chrysovirus 1
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D6QX90|D6QX90_9VIRU Putative replication associated protein OS=Cryphonectria nitschkei chrysovirus 1 OX=399394 PE=4 SV=1
MM1 pKa = 7.24 SAYY4 pKa = 9.42 KK5 pKa = 10.1 RR6 pKa = 11.84 VMDD9 pKa = 4.19 YY10 pKa = 10.74 MSGAPDD16 pKa = 3.42 SANHH20 pKa = 5.52 IKK22 pKa = 10.42 LWEE25 pKa = 4.18 KK26 pKa = 10.88 AQMDD30 pKa = 4.02 RR31 pKa = 11.84 MRR33 pKa = 11.84 DD34 pKa = 3.38 QVDD37 pKa = 3.07 ALEE40 pKa = 4.32 LEE42 pKa = 4.19 KK43 pKa = 11.1 RR44 pKa = 11.84 MLGPAEE50 pKa = 4.57 RR51 pKa = 11.84 DD52 pKa = 2.97 LMAAFHH58 pKa = 6.29 CQPKK62 pKa = 8.54 FTRR65 pKa = 11.84 KK66 pKa = 9.38 GMNEE70 pKa = 3.7 GLTIGVNVDD79 pKa = 3.24 CRR81 pKa = 11.84 TSSGDD86 pKa = 3.11 KK87 pKa = 10.11 VSRR90 pKa = 11.84 IKK92 pKa = 11.1 NVVVDD97 pKa = 5.03 FNGGEE102 pKa = 4.23 CWEE105 pKa = 4.3 IAHH108 pKa = 6.91 AAAHH112 pKa = 5.79 VDD114 pKa = 3.62 YY115 pKa = 10.04 VTRR118 pKa = 11.84 DD119 pKa = 3.32 PAFYY123 pKa = 10.17 TLDD126 pKa = 3.03 TTLRR130 pKa = 11.84 DD131 pKa = 4.24 KK132 pKa = 10.82 IISQCPGMSAAQLYY146 pKa = 6.91 EE147 pKa = 3.83 LRR149 pKa = 11.84 VDD151 pKa = 3.84 GVKK154 pKa = 10.65 DD155 pKa = 3.79 SLSLPVDD162 pKa = 3.38 MRR164 pKa = 11.84 HH165 pKa = 5.87 MYY167 pKa = 9.73 MKK169 pKa = 10.1 MMLMLWDD176 pKa = 3.6 YY177 pKa = 10.5 TLAATSKK184 pKa = 9.88 VEE186 pKa = 4.05 GMEE189 pKa = 3.97 MAEE192 pKa = 4.59 DD193 pKa = 3.5 IVDD196 pKa = 4.81 FSHH199 pKa = 7.65 PSLPADD205 pKa = 2.98 ARR207 pKa = 11.84 MATVANYY214 pKa = 10.08 RR215 pKa = 11.84 VVVDD219 pKa = 3.62 AEE221 pKa = 4.3 GMSRR225 pKa = 11.84 RR226 pKa = 11.84 EE227 pKa = 3.96 LGLLSMAGQQYY238 pKa = 9.8 PSVWYY243 pKa = 10.27 AGDD246 pKa = 3.87 NIYY249 pKa = 9.43 TKK251 pKa = 10.66 CHH253 pKa = 5.72 LAEE256 pKa = 5.25 DD257 pKa = 3.93 SLAIVSDD264 pKa = 3.69 GKK266 pKa = 10.61 IDD268 pKa = 3.73 MDD270 pKa = 3.99 TSGMWGSPDD279 pKa = 3.35 EE280 pKa = 4.85 LYY282 pKa = 11.1 SIIWSIACKK291 pKa = 9.83 TSSTKK296 pKa = 10.53 CLAEE300 pKa = 4.98 AITLLRR306 pKa = 11.84 GKK308 pKa = 8.62 CQHH311 pKa = 6.28 MADD314 pKa = 3.67 FTKK317 pKa = 10.4 RR318 pKa = 11.84 AGPITVQSGVPLSVCKK334 pKa = 10.53 VRR336 pKa = 11.84 SVGAASASTILQSQPGYY353 pKa = 10.55 FSTSLSLVTDD363 pKa = 4.06 LLYY366 pKa = 11.28 GKK368 pKa = 8.9 MFEE371 pKa = 4.18 ISATSVVEE379 pKa = 3.98 QVGGLGDD386 pKa = 3.79 RR387 pKa = 11.84 TCGEE391 pKa = 4.27 VPSTDD396 pKa = 2.75 RR397 pKa = 11.84 MYY399 pKa = 11.33 NGLLRR404 pKa = 11.84 DD405 pKa = 2.99 IGLRR409 pKa = 11.84 HH410 pKa = 6.61 EE411 pKa = 5.34 DD412 pKa = 3.37 GSINAVLKK420 pKa = 9.88 NWCSLTGSAYY430 pKa = 8.73 TFGYY434 pKa = 10.06 GGAIKK439 pKa = 10.3 DD440 pKa = 3.83 YY441 pKa = 10.54 VIKK444 pKa = 9.83 LTEE447 pKa = 4.04 LMRR450 pKa = 11.84 GGWDD454 pKa = 3.26 VEE456 pKa = 4.22 LPQLGTLIPFMDD468 pKa = 4.37 TKK470 pKa = 9.97 DD471 pKa = 3.64 TCWGYY476 pKa = 11.27 SRR478 pKa = 11.84 GYY480 pKa = 8.78 PGKK483 pKa = 9.54 PDD485 pKa = 4.35 VFDD488 pKa = 4.95 KK489 pKa = 11.03 DD490 pKa = 3.53 QTMKK494 pKa = 10.8 ASEE497 pKa = 4.31 SLSVCAWLMGLRR509 pKa = 11.84 ATRR512 pKa = 11.84 PKK514 pKa = 10.47 VGHH517 pKa = 6.01 NRR519 pKa = 11.84 SKK521 pKa = 10.87 KK522 pKa = 8.43 EE523 pKa = 3.9 SRR525 pKa = 11.84 HH526 pKa = 3.76 QTPEE530 pKa = 3.61 EE531 pKa = 3.93 RR532 pKa = 11.84 EE533 pKa = 3.9 LAVEE537 pKa = 4.51 SKK539 pKa = 11.07 GKK541 pKa = 8.98 MVLGSAQLWITDD553 pKa = 3.69 GLGGRR558 pKa = 11.84 EE559 pKa = 3.95 DD560 pKa = 4.49 DD561 pKa = 3.99 YY562 pKa = 11.98 EE563 pKa = 4.39 EE564 pKa = 4.93 GSGVLVKK571 pKa = 10.77 SSFPSVKK578 pKa = 10.22 CQLLYY583 pKa = 10.78 DD584 pKa = 3.98 EE585 pKa = 6.1 RR586 pKa = 11.84 DD587 pKa = 3.89 QEE589 pKa = 4.14 WHH591 pKa = 6.52 IPTSRR596 pKa = 11.84 EE597 pKa = 3.1 RR598 pKa = 11.84 DD599 pKa = 3.5 YY600 pKa = 11.38 TSMVRR605 pKa = 11.84 RR606 pKa = 11.84 TTMGEE611 pKa = 4.03 HH612 pKa = 5.58 EE613 pKa = 4.67 VEE615 pKa = 4.26 VAPVIVGPAPKK626 pKa = 10.14 SPEE629 pKa = 4.48 DD630 pKa = 3.24 IFSGVRR636 pKa = 11.84 AAVKK640 pKa = 9.2 EE641 pKa = 4.22 RR642 pKa = 11.84 PVRR645 pKa = 11.84 PYY647 pKa = 10.62 KK648 pKa = 10.32 EE649 pKa = 3.86 PRR651 pKa = 11.84 PAVGMTGVKK660 pKa = 9.83 FVPRR664 pKa = 11.84 YY665 pKa = 9.27 VLDD668 pKa = 4.1 GKK670 pKa = 10.42 VVEE673 pKa = 4.98 DD674 pKa = 5.05 PIDD677 pKa = 3.55 VRR679 pKa = 11.84 TKK681 pKa = 10.47 RR682 pKa = 11.84 PATGEE687 pKa = 4.06 MRR689 pKa = 11.84 VKK691 pKa = 10.78 AIDD694 pKa = 3.42 VPGDD698 pKa = 3.96 GKK700 pKa = 11.01 CGLHH704 pKa = 6.86 AVVQDD709 pKa = 4.09 LQSHH713 pKa = 6.38 GLLTQRR719 pKa = 11.84 DD720 pKa = 3.89 GGRR723 pKa = 11.84 VLRR726 pKa = 11.84 LLDD729 pKa = 3.75 KK730 pKa = 10.23 STNAPTFHH738 pKa = 7.26 SPEE741 pKa = 5.51 DD742 pKa = 3.34 IAGAVLKK749 pKa = 10.77 LGLGLDD755 pKa = 3.86 VVAGGIVHH763 pKa = 7.12 SYY765 pKa = 10.23 GNNDD769 pKa = 3.04 GHH771 pKa = 6.46 RR772 pKa = 11.84 VLMQLKK778 pKa = 8.77 DD779 pKa = 3.49 HH780 pKa = 7.04 HH781 pKa = 6.97 YY782 pKa = 8.64 MPIVEE787 pKa = 4.41 HH788 pKa = 6.91 EE789 pKa = 4.37 EE790 pKa = 4.58 GEE792 pKa = 4.52 TLNITSVQEE801 pKa = 3.93 QVGTDD806 pKa = 3.21 DD807 pKa = 4.99 EE808 pKa = 4.91 YY809 pKa = 10.95 IKK811 pKa = 10.86 RR812 pKa = 11.84 LGEE815 pKa = 4.07 FEE817 pKa = 4.79 RR818 pKa = 11.84 MLAAATT824 pKa = 3.76
Molecular weight: 90.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.566
IPC2_protein 5.525
IPC_protein 5.55
Toseland 5.855
ProMoST 5.855
Dawson 5.753
Bjellqvist 5.792
Wikipedia 5.728
Rodwell 5.715
Grimsley 5.982
Solomon 5.753
Lehninger 5.728
Nozaki 5.982
DTASelect 6.173
Thurlkill 6.122
EMBOSS 6.084
Sillero 6.084
Patrickios 4.164
IPC_peptide 5.766
IPC2_peptide 6.084
IPC2.peptide.svr19 6.04
Protein with the highest isoelectric point:
>tr|D6QX90|D6QX90_9VIRU Putative replication associated protein OS=Cryphonectria nitschkei chrysovirus 1 OX=399394 PE=4 SV=1
MM1 pKa = 7.53 SISPNSRR8 pKa = 11.84 YY9 pKa = 10.31 AGGGGHH15 pKa = 5.24 GHH17 pKa = 6.52 KK18 pKa = 10.64 VGLAEE23 pKa = 3.9 KK24 pKa = 10.18 AARR27 pKa = 11.84 RR28 pKa = 11.84 LINRR32 pKa = 11.84 GKK34 pKa = 8.69 VTYY37 pKa = 10.01 GPKK40 pKa = 10.44 DD41 pKa = 3.75 FEE43 pKa = 4.82 VKK45 pKa = 10.67 DD46 pKa = 3.58 SDD48 pKa = 3.79 RR49 pKa = 11.84 NLIGVVIPSLLGKK62 pKa = 6.83 TTLANQFGWLDD73 pKa = 3.58 VDD75 pKa = 6.25 DD76 pKa = 5.08 LTTDD80 pKa = 3.54 DD81 pKa = 5.46 KK82 pKa = 11.61 RR83 pKa = 11.84 NQLFATFSQRR93 pKa = 11.84 YY94 pKa = 9.09 AEE96 pKa = 5.1 DD97 pKa = 3.62 GWCAAMRR104 pKa = 11.84 PFNEE108 pKa = 4.08 EE109 pKa = 3.7 CEE111 pKa = 4.04 ASIDD115 pKa = 3.44 RR116 pKa = 11.84 MYY118 pKa = 10.67 FYY120 pKa = 11.0 SQTVILAHH128 pKa = 6.07 SVSSLAYY135 pKa = 9.98 LGVIPSIRR143 pKa = 11.84 IAPSDD148 pKa = 3.69 DD149 pKa = 3.31 FAAKK153 pKa = 10.25 LLSLEE158 pKa = 4.48 TGGSAAFAKK167 pKa = 10.73 ANIEE171 pKa = 4.06 AVRR174 pKa = 11.84 EE175 pKa = 4.11 EE176 pKa = 4.66 ADD178 pKa = 3.38 SSPLVTVSSYY188 pKa = 11.87 DD189 pKa = 3.65 EE190 pKa = 4.41 LSATAVQLAIRR201 pKa = 11.84 LGHH204 pKa = 7.03 DD205 pKa = 3.58 VDD207 pKa = 5.08 CKK209 pKa = 10.97 AVSQDD214 pKa = 3.67 DD215 pKa = 5.64 AIDD218 pKa = 3.53 QYY220 pKa = 11.87 EE221 pKa = 4.32 CGLIEE226 pKa = 5.89 RR227 pKa = 11.84 DD228 pKa = 3.52 DD229 pKa = 3.93 ADD231 pKa = 3.9 RR232 pKa = 11.84 LVRR235 pKa = 11.84 EE236 pKa = 4.2 HH237 pKa = 6.95 GGLHH241 pKa = 6.33 RR242 pKa = 11.84 GFGRR246 pKa = 11.84 TRR248 pKa = 11.84 SGWARR253 pKa = 11.84 AVACIGCDD261 pKa = 3.27 NKK263 pKa = 10.5 PALSVTVDD271 pKa = 3.61 KK272 pKa = 11.17 EE273 pKa = 4.4 VLDD276 pKa = 3.98 KK277 pKa = 11.48 ANCINRR283 pKa = 11.84 AVVGRR288 pKa = 11.84 ILTKK292 pKa = 10.28 SNKK295 pKa = 8.77 TSAHH299 pKa = 4.46 VQALLTFVVAFGKK312 pKa = 10.56 GQLLDD317 pKa = 4.02 VISNLLTTPEE327 pKa = 4.23 SDD329 pKa = 2.93 WEE331 pKa = 4.0 RR332 pKa = 11.84 VMRR335 pKa = 11.84 GISSLVKK342 pKa = 9.9 TSNSYY347 pKa = 9.32 MGYY350 pKa = 10.41 DD351 pKa = 3.27 LSDD354 pKa = 3.56 DD355 pKa = 3.59 EE356 pKa = 4.54 RR357 pKa = 11.84 RR358 pKa = 11.84 VMSEE362 pKa = 3.42 LWLLGHH368 pKa = 6.47 QNRR371 pKa = 11.84 TSMAALMRR379 pKa = 11.84 EE380 pKa = 4.0 WLLGCGGTYY389 pKa = 10.5 KK390 pKa = 10.77 SIPTSADD397 pKa = 2.36 IRR399 pKa = 11.84 KK400 pKa = 8.97 AADD403 pKa = 3.39 GAGLAGGRR411 pKa = 11.84 TVRR414 pKa = 11.84 QSLQQALKK422 pKa = 8.88 MWAEE426 pKa = 4.33 TWRR429 pKa = 11.84 LSDD432 pKa = 3.61 KK433 pKa = 11.24 ASLEE437 pKa = 3.94 ALQGVDD443 pKa = 3.88 LAFGNASGTSWYY455 pKa = 9.29 KK456 pKa = 9.51 ACRR459 pKa = 11.84 IIYY462 pKa = 7.73 LTYY465 pKa = 10.01 EE466 pKa = 3.78 SDD468 pKa = 5.45 EE469 pKa = 4.18 IGEE472 pKa = 4.26 WLSKK476 pKa = 10.28 LVSVRR481 pKa = 11.84 EE482 pKa = 3.84 QDD484 pKa = 3.4 VRR486 pKa = 11.84 EE487 pKa = 4.12 VDD489 pKa = 2.78 WKK491 pKa = 10.98 RR492 pKa = 11.84 RR493 pKa = 11.84 VEE495 pKa = 4.24 SAVSSLLLNLLAGAIGGIEE514 pKa = 4.18 VEE516 pKa = 4.28 KK517 pKa = 10.68 HH518 pKa = 4.83 SCPVRR523 pKa = 11.84 ASDD526 pKa = 4.95 CDD528 pKa = 3.57 ALSAVILMMGIRR540 pKa = 11.84 QVEE543 pKa = 4.27 RR544 pKa = 11.84 DD545 pKa = 3.5 TGGLTTWAAGRR556 pKa = 11.84 IAEE559 pKa = 4.51 TMTMMEE565 pKa = 4.61 SKK567 pKa = 10.62 VVTAIEE573 pKa = 4.16 ASLIMSSGDD582 pKa = 3.25 KK583 pKa = 10.82 AHH585 pKa = 6.71 EE586 pKa = 3.76 LWANIWQRR594 pKa = 11.84 GAIYY598 pKa = 10.14 HH599 pKa = 5.63 MPGMMWMARR608 pKa = 11.84 HH609 pKa = 5.89 KK610 pKa = 10.22 FDD612 pKa = 4.03 HH613 pKa = 6.43 KK614 pKa = 10.6 RR615 pKa = 11.84 RR616 pKa = 11.84 SDD618 pKa = 3.83 GYY620 pKa = 9.51 IKK622 pKa = 10.57 AYY624 pKa = 9.9 LARR627 pKa = 11.84 LCSPKK632 pKa = 10.66 SKK634 pKa = 10.42 GGSGRR639 pKa = 11.84 VSMTGSTFHH648 pKa = 7.53 PEE650 pKa = 3.75 QRR652 pKa = 11.84 WSGKK656 pKa = 9.52 HH657 pKa = 4.41 KK658 pKa = 10.59 QQGEE662 pKa = 3.85 WRR664 pKa = 11.84 RR665 pKa = 11.84 ASGSTSLVEE674 pKa = 3.73 LRR676 pKa = 11.84 GIEE679 pKa = 4.09 DD680 pKa = 3.82 VPTISCGEE688 pKa = 4.18 AIKK691 pKa = 9.46 STKK694 pKa = 10.25 AVALGLIGSCITQGLPAKK712 pKa = 9.5 KK713 pKa = 9.12 VCVVLGRR720 pKa = 11.84 LLAKK724 pKa = 10.17 RR725 pKa = 11.84 RR726 pKa = 11.84 QVLIGKK732 pKa = 7.59 VEE734 pKa = 4.01 KK735 pKa = 11.06 VEE737 pKa = 4.48 EE738 pKa = 4.51 PEE740 pKa = 3.96 QQLL743 pKa = 3.46
Molecular weight: 81.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.078
IPC2_protein 7.512
IPC_protein 7.322
Toseland 7.0
ProMoST 7.936
Dawson 8.053
Bjellqvist 8.463
Wikipedia 7.966
Rodwell 8.083
Grimsley 7.059
Solomon 8.097
Lehninger 8.112
Nozaki 8.726
DTASelect 8.185
Thurlkill 8.244
EMBOSS 8.273
Sillero 8.58
Patrickios 4.584
IPC_peptide 8.097
IPC2_peptide 7.454
IPC2.peptide.svr19 7.506
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3435
743
962
858.8
95.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.21 ± 0.461
1.456 ± 0.209
6.143 ± 0.511
6.376 ± 0.171
2.591 ± 0.543
7.54 ± 0.685
2.416 ± 0.298
4.6 ± 0.214
5.007 ± 0.327
9.054 ± 0.219
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.61 ± 0.314
2.969 ± 0.443
3.785 ± 0.406
2.882 ± 0.108
6.9 ± 0.254
8.21 ± 0.539
5.56 ± 0.288
7.831 ± 0.411
1.805 ± 0.217
3.057 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here