Amino acid dipepetide frequency for Nitrospira sp. KM1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.223AlaAla: 10.223 ± 0.108
1.054AlaCys: 1.054 ± 0.035
4.854AlaAsp: 4.854 ± 0.068
5.48AlaGlu: 5.48 ± 0.074
3.433AlaPhe: 3.433 ± 0.054
8.224AlaGly: 8.224 ± 0.101
2.026AlaHis: 2.026 ± 0.046
5.213AlaIle: 5.213 ± 0.076
3.981AlaLys: 3.981 ± 0.066
10.026AlaLeu: 10.026 ± 0.123
2.747AlaMet: 2.747 ± 0.048
2.553AlaAsn: 2.553 ± 0.061
3.877AlaPro: 3.877 ± 0.065
3.498AlaGln: 3.498 ± 0.063
5.919AlaArg: 5.919 ± 0.08
5.706AlaSer: 5.706 ± 0.073
4.908AlaThr: 4.908 ± 0.076
7.735AlaVal: 7.735 ± 0.087
1.247AlaTrp: 1.247 ± 0.038
2.404AlaTyr: 2.404 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
0.892CysAla: 0.892 ± 0.026
0.14CysCys: 0.14 ± 0.013
0.462CysAsp: 0.462 ± 0.019
0.489CysGlu: 0.489 ± 0.019
0.339CysPhe: 0.339 ± 0.018
0.902CysGly: 0.902 ± 0.03
0.351CysHis: 0.351 ± 0.021
0.436CysIle: 0.436 ± 0.021
0.274CysLys: 0.274 ± 0.016
1.091CysLeu: 1.091 ± 0.033
0.203CysMet: 0.203 ± 0.013
0.253CysAsn: 0.253 ± 0.016
0.562CysPro: 0.562 ± 0.023
0.312CysGln: 0.312 ± 0.017
0.809CysArg: 0.809 ± 0.03
0.613CysSer: 0.613 ± 0.025
0.482CysThr: 0.482 ± 0.023
0.672CysVal: 0.672 ± 0.026
0.092CysTrp: 0.092 ± 0.008
0.282CysTyr: 0.282 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
4.746AspAla: 4.746 ± 0.059
0.525AspCys: 0.525 ± 0.021
2.542AspAsp: 2.542 ± 0.054
3.223AspGlu: 3.223 ± 0.05
1.86AspPhe: 1.86 ± 0.039
4.153AspGly: 4.153 ± 0.066
1.465AspHis: 1.465 ± 0.034
2.86AspIle: 2.86 ± 0.047
1.761AspLys: 1.761 ± 0.043
5.838AspLeu: 5.838 ± 0.081
1.134AspMet: 1.134 ± 0.028
1.316AspAsn: 1.316 ± 0.035
3.239AspPro: 3.239 ± 0.057
2.227AspGln: 2.227 ± 0.046
4.347AspArg: 4.347 ± 0.066
2.737AspSer: 2.737 ± 0.051
2.51AspThr: 2.51 ± 0.061
4.222AspVal: 4.222 ± 0.068
0.769AspTrp: 0.769 ± 0.024
1.477AspTyr: 1.477 ± 0.032
0.0AspXaa: 0.0 ± 0.0
Glu
5.932GluAla: 5.932 ± 0.084
0.475GluCys: 0.475 ± 0.019
2.662GluAsp: 2.662 ± 0.05
3.702GluGlu: 3.702 ± 0.071
2.072GluPhe: 2.072 ± 0.037
4.064GluGly: 4.064 ± 0.068
1.521GluHis: 1.521 ± 0.036
3.319GluIle: 3.319 ± 0.052
2.507GluLys: 2.507 ± 0.052
5.938GluLeu: 5.938 ± 0.077
1.447GluMet: 1.447 ± 0.035
1.355GluAsn: 1.355 ± 0.036
2.436GluPro: 2.436 ± 0.046
2.914GluGln: 2.914 ± 0.046
4.979GluArg: 4.979 ± 0.07
3.503GluSer: 3.503 ± 0.061
3.227GluThr: 3.227 ± 0.052
4.063GluVal: 4.063 ± 0.064
0.853GluTrp: 0.853 ± 0.027
1.433GluTyr: 1.433 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
3.233PheAla: 3.233 ± 0.054
0.404PheCys: 0.404 ± 0.019
2.356PheAsp: 2.356 ± 0.045
2.155PheGlu: 2.155 ± 0.045
1.513PhePhe: 1.513 ± 0.04
3.214PheGly: 3.214 ± 0.056
0.963PheHis: 0.963 ± 0.026
1.752PheIle: 1.752 ± 0.038
1.293PheLys: 1.293 ± 0.037
3.792PheLeu: 3.792 ± 0.062
0.888PheMet: 0.888 ± 0.022
1.231PheAsn: 1.231 ± 0.036
1.765PhePro: 1.765 ± 0.039
1.301PheGln: 1.301 ± 0.035
2.375PheArg: 2.375 ± 0.04
2.475PheSer: 2.475 ± 0.048
2.188PheThr: 2.188 ± 0.048
2.798PheVal: 2.798 ± 0.055
0.518PheTrp: 0.518 ± 0.022
1.078PheTyr: 1.078 ± 0.034
0.0PheXaa: 0.0 ± 0.0
Gly
6.674GlyAla: 6.674 ± 0.093
0.943GlyCys: 0.943 ± 0.03
3.697GlyAsp: 3.697 ± 0.068
4.237GlyGlu: 4.237 ± 0.062
3.262GlyPhe: 3.262 ± 0.055
6.315GlyGly: 6.315 ± 0.129
1.902GlyHis: 1.902 ± 0.042
4.665GlyIle: 4.665 ± 0.069
3.628GlyLys: 3.628 ± 0.052
8.139GlyLeu: 8.139 ± 0.097
2.222GlyMet: 2.222 ± 0.048
2.412GlyAsn: 2.412 ± 0.088
3.075GlyPro: 3.075 ± 0.056
3.072GlyGln: 3.072 ± 0.051
5.401GlyArg: 5.401 ± 0.062
5.091GlySer: 5.091 ± 0.073
5.061GlyThr: 5.061 ± 0.087
5.696GlyVal: 5.696 ± 0.07
1.218GlyTrp: 1.218 ± 0.033
2.389GlyTyr: 2.389 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
2.319HisAla: 2.319 ± 0.04
0.308HisCys: 0.308 ± 0.016
1.358HisAsp: 1.358 ± 0.036
1.453HisGlu: 1.453 ± 0.039
0.881HisPhe: 0.881 ± 0.028
1.951HisGly: 1.951 ± 0.044
0.776HisHis: 0.776 ± 0.031
1.168HisIle: 1.168 ± 0.028
0.675HisLys: 0.675 ± 0.023
2.469HisLeu: 2.469 ± 0.046
0.562HisMet: 0.562 ± 0.023
0.56HisAsn: 0.56 ± 0.019
1.537HisPro: 1.537 ± 0.035
0.966HisGln: 0.966 ± 0.03
1.788HisArg: 1.788 ± 0.039
1.219HisSer: 1.219 ± 0.034
1.164HisThr: 1.164 ± 0.034
1.878HisVal: 1.878 ± 0.043
0.33HisTrp: 0.33 ± 0.015
0.667HisTyr: 0.667 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
5.42IleAla: 5.42 ± 0.071
0.502IleCys: 0.502 ± 0.023
3.241IleAsp: 3.241 ± 0.05
3.637IleGlu: 3.637 ± 0.059
1.677IlePhe: 1.677 ± 0.04
4.783IleGly: 4.783 ± 0.068
1.263IleHis: 1.263 ± 0.034
2.455IleIle: 2.455 ± 0.05
2.099IleLys: 2.099 ± 0.045
4.966IleLeu: 4.966 ± 0.079
1.062IleMet: 1.062 ± 0.03
1.585IleAsn: 1.585 ± 0.039
3.073IlePro: 3.073 ± 0.05
1.95IleGln: 1.95 ± 0.04
3.847IleArg: 3.847 ± 0.057
3.083IleSer: 3.083 ± 0.057
2.997IleThr: 2.997 ± 0.054
4.458IleVal: 4.458 ± 0.067
0.563IleTrp: 0.563 ± 0.023
1.182IleTyr: 1.182 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
3.826LysAla: 3.826 ± 0.062
0.228LysCys: 0.228 ± 0.014
2.387LysAsp: 2.387 ± 0.048
2.675LysGlu: 2.675 ± 0.056
1.117LysPhe: 1.117 ± 0.031
3.028LysGly: 3.028 ± 0.055
0.925LysHis: 0.925 ± 0.029
2.172LysIle: 2.172 ± 0.043
2.032LysLys: 2.032 ± 0.057
3.563LysLeu: 3.563 ± 0.058
0.992LysMet: 0.992 ± 0.029
1.196LysAsn: 1.196 ± 0.032
2.171LysPro: 2.171 ± 0.043
1.795LysGln: 1.795 ± 0.038
2.751LysArg: 2.751 ± 0.052
2.365LysSer: 2.365 ± 0.041
2.533LysThr: 2.533 ± 0.044
2.766LysVal: 2.766 ± 0.044
0.422LysTrp: 0.422 ± 0.021
0.899LysTyr: 0.899 ± 0.03
0.0LysXaa: 0.0 ± 0.0
Leu
10.197LeuAla: 10.197 ± 0.111
1.087LeuCys: 1.087 ± 0.029
5.475LeuAsp: 5.475 ± 0.069
5.774LeuGlu: 5.774 ± 0.076
3.917LeuPhe: 3.917 ± 0.064
7.428LeuGly: 7.428 ± 0.092
2.338LeuHis: 2.338 ± 0.046
5.344LeuIle: 5.344 ± 0.071
4.384LeuLys: 4.384 ± 0.061
10.349LeuLeu: 10.349 ± 0.139
2.362LeuMet: 2.362 ± 0.044
3.195LeuAsn: 3.195 ± 0.053
5.258LeuPro: 5.258 ± 0.075
3.757LeuGln: 3.757 ± 0.057
6.813LeuArg: 6.813 ± 0.094
6.989LeuSer: 6.989 ± 0.078
6.421LeuThr: 6.421 ± 0.086
7.212LeuVal: 7.212 ± 0.09
1.21LeuTrp: 1.21 ± 0.034
2.744LeuTyr: 2.744 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
2.623MetAla: 2.623 ± 0.046
0.176MetCys: 0.176 ± 0.012
1.241MetAsp: 1.241 ± 0.032
1.288MetGlu: 1.288 ± 0.032
0.754MetPhe: 0.754 ± 0.025
1.761MetGly: 1.761 ± 0.038
0.475MetHis: 0.475 ± 0.017
1.358MetIle: 1.358 ± 0.029
1.391MetLys: 1.391 ± 0.035
2.339MetLeu: 2.339 ± 0.041
0.669MetMet: 0.669 ± 0.027
0.945MetAsn: 0.945 ± 0.027
1.422MetPro: 1.422 ± 0.031
0.899MetGln: 0.899 ± 0.027
1.666MetArg: 1.666 ± 0.034
1.588MetSer: 1.588 ± 0.041
1.85MetThr: 1.85 ± 0.035
1.842MetVal: 1.842 ± 0.043
0.243MetTrp: 0.243 ± 0.014
0.451MetTyr: 0.451 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
2.604AsnAla: 2.604 ± 0.053
0.263AsnCys: 0.263 ± 0.015
1.457AsnAsp: 1.457 ± 0.054
1.419AsnGlu: 1.419 ± 0.034
1.044AsnPhe: 1.044 ± 0.036
2.623AsnGly: 2.623 ± 0.06
0.656AsnHis: 0.656 ± 0.022
1.531AsnIle: 1.531 ± 0.043
0.961AsnLys: 0.961 ± 0.027
3.043AsnLeu: 3.043 ± 0.054
0.676AsnMet: 0.676 ± 0.025
0.928AsnAsn: 0.928 ± 0.037
2.064AsnPro: 2.064 ± 0.046
1.158AsnGln: 1.158 ± 0.031
2.176AsnArg: 2.176 ± 0.041
1.527AsnSer: 1.527 ± 0.043
1.45AsnThr: 1.45 ± 0.04
2.248AsnVal: 2.248 ± 0.05
0.423AsnTrp: 0.423 ± 0.02
0.782AsnTyr: 0.782 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
4.93ProAla: 4.93 ± 0.071
0.381ProCys: 0.381 ± 0.018
3.352ProAsp: 3.352 ± 0.054
3.193ProGlu: 3.193 ± 0.052
2.033ProPhe: 2.033 ± 0.042
4.084ProGly: 4.084 ± 0.063
1.21ProHis: 1.21 ± 0.033
2.561ProIle: 2.561 ± 0.046
1.814ProLys: 1.814 ± 0.038
4.821ProLeu: 4.821 ± 0.063
1.22ProMet: 1.22 ± 0.033
1.524ProAsn: 1.524 ± 0.037
2.595ProPro: 2.595 ± 0.061
1.789ProGln: 1.789 ± 0.036
2.546ProArg: 2.546 ± 0.054
3.498ProSer: 3.498 ± 0.055
2.644ProThr: 2.644 ± 0.043
4.19ProVal: 4.19 ± 0.064
0.659ProTrp: 0.659 ± 0.027
1.301ProTyr: 1.301 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
4.15GlnAla: 4.15 ± 0.073
0.292GlnCys: 0.292 ± 0.016
1.976GlnAsp: 1.976 ± 0.043
2.394GlnGlu: 2.394 ± 0.048
1.449GlnPhe: 1.449 ± 0.038
2.816GlnGly: 2.816 ± 0.051
0.938GlnHis: 0.938 ± 0.029
2.023GlnIle: 2.023 ± 0.042
1.401GlnLys: 1.401 ± 0.037
3.813GlnLeu: 3.813 ± 0.065
0.903GlnMet: 0.903 ± 0.026
1.005GlnAsn: 1.005 ± 0.029
1.997GlnPro: 1.997 ± 0.042
1.867GlnGln: 1.867 ± 0.046
2.796GlnArg: 2.796 ± 0.05
2.473GlnSer: 2.473 ± 0.046
2.197GlnThr: 2.197 ± 0.046
2.881GlnVal: 2.881 ± 0.049
0.583GlnTrp: 0.583 ± 0.022
0.991GlnTyr: 0.991 ± 0.031
0.0GlnXaa: 0.0 ± 0.0
Arg
5.83ArgAla: 5.83 ± 0.061
0.624ArgCys: 0.624 ± 0.026
3.608ArgAsp: 3.608 ± 0.059
4.417ArgGlu: 4.417 ± 0.067
2.81ArgPhe: 2.81 ± 0.054
4.228ArgGly: 4.228 ± 0.057
1.83ArgHis: 1.83 ± 0.039
4.135ArgIle: 4.135 ± 0.057
2.591ArgLys: 2.591 ± 0.046
7.767ArgLeu: 7.767 ± 0.096
2.031ArgMet: 2.031 ± 0.043
1.96ArgAsn: 1.96 ± 0.035
3.1ArgPro: 3.1 ± 0.046
2.919ArgGln: 2.919 ± 0.051
5.12ArgArg: 5.12 ± 0.076
4.191ArgSer: 4.191 ± 0.058
3.923ArgThr: 3.923 ± 0.058
4.857ArgVal: 4.857 ± 0.074
1.093ArgTrp: 1.093 ± 0.028
2.135ArgTyr: 2.135 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
5.603SerAla: 5.603 ± 0.079
0.606SerCys: 0.606 ± 0.025
3.221SerAsp: 3.221 ± 0.052
3.219SerGlu: 3.219 ± 0.052
2.4SerPhe: 2.4 ± 0.043
5.569SerGly: 5.569 ± 0.076
1.475SerHis: 1.475 ± 0.03
3.297SerIle: 3.297 ± 0.052
2.18SerLys: 2.18 ± 0.048
6.382SerLeu: 6.382 ± 0.074
1.638SerMet: 1.638 ± 0.038
1.72SerAsn: 1.72 ± 0.046
3.293SerPro: 3.293 ± 0.059
2.239SerGln: 2.239 ± 0.042
4.195SerArg: 4.195 ± 0.062
4.187SerSer: 4.187 ± 0.069
3.348SerThr: 3.348 ± 0.056
4.66SerVal: 4.66 ± 0.057
0.897SerTrp: 0.897 ± 0.029
1.635SerTyr: 1.635 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
5.562ThrAla: 5.562 ± 0.071
0.485ThrCys: 0.485 ± 0.023
2.965ThrAsp: 2.965 ± 0.048
2.835ThrGlu: 2.835 ± 0.053
2.232ThrPhe: 2.232 ± 0.05
5.219ThrGly: 5.219 ± 0.075
1.253ThrHis: 1.253 ± 0.033
3.481ThrIle: 3.481 ± 0.057
2.166ThrLys: 2.166 ± 0.04
5.761ThrLeu: 5.761 ± 0.084
1.368ThrMet: 1.368 ± 0.031
1.652ThrAsn: 1.652 ± 0.043
3.165ThrPro: 3.165 ± 0.054
1.849ThrGln: 1.849 ± 0.043
3.219ThrArg: 3.219 ± 0.05
3.269ThrSer: 3.269 ± 0.056
3.316ThrThr: 3.316 ± 0.057
5.26ThrVal: 5.26 ± 0.07
0.729ThrTrp: 0.729 ± 0.027
1.507ThrTyr: 1.507 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
7.024ValAla: 7.024 ± 0.091
0.715ValCys: 0.715 ± 0.024
3.826ValAsp: 3.826 ± 0.061
4.543ValGlu: 4.543 ± 0.071
2.782ValPhe: 2.782 ± 0.049
5.654ValGly: 5.654 ± 0.068
1.614ValHis: 1.614 ± 0.041
4.279ValIle: 4.279 ± 0.066
3.199ValLys: 3.199 ± 0.051
7.864ValLeu: 7.864 ± 0.096
1.923ValMet: 1.923 ± 0.043
2.362ValAsn: 2.362 ± 0.055
3.889ValPro: 3.889 ± 0.056
2.756ValGln: 2.756 ± 0.054
5.201ValArg: 5.201 ± 0.067
4.934ValSer: 4.934 ± 0.059
5.013ValThr: 5.013 ± 0.075
5.996ValVal: 5.996 ± 0.086
0.913ValTrp: 0.913 ± 0.024
1.722ValTyr: 1.722 ± 0.04
0.0ValXaa: 0.0 ± 0.0
Trp
1.034TrpAla: 1.034 ± 0.029
0.149TrpCys: 0.149 ± 0.011
0.663TrpAsp: 0.663 ± 0.024
0.744TrpGlu: 0.744 ± 0.028
0.584TrpPhe: 0.584 ± 0.02
0.899TrpGly: 0.899 ± 0.033
0.347TrpHis: 0.347 ± 0.019
0.749TrpIle: 0.749 ± 0.027
0.685TrpLys: 0.685 ± 0.028
1.531TrpLeu: 1.531 ± 0.035
0.397TrpMet: 0.397 ± 0.016
0.503TrpAsn: 0.503 ± 0.019
0.556TrpPro: 0.556 ± 0.019
0.574TrpGln: 0.574 ± 0.022
0.847TrpArg: 0.847 ± 0.029
0.888TrpSer: 0.888 ± 0.03
0.775TrpThr: 0.775 ± 0.023
0.869TrpVal: 0.869 ± 0.029
0.263TrpTrp: 0.263 ± 0.017
0.373TrpTyr: 0.373 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.287TyrAla: 2.287 ± 0.047
0.299TyrCys: 0.299 ± 0.016
1.606TyrAsp: 1.606 ± 0.045
1.528TyrGlu: 1.528 ± 0.037
1.127TyrPhe: 1.127 ± 0.035
2.186TyrGly: 2.186 ± 0.042
0.701TyrHis: 0.701 ± 0.028
1.044TyrIle: 1.044 ± 0.028
0.821TyrLys: 0.821 ± 0.028
2.761TyrLeu: 2.761 ± 0.056
0.539TyrMet: 0.539 ± 0.021
0.78TyrAsn: 0.78 ± 0.028
1.272TyrPro: 1.272 ± 0.033
1.105TyrGln: 1.105 ± 0.027
2.372TyrArg: 2.372 ± 0.045
1.43TyrSer: 1.43 ± 0.034
1.287TyrThr: 1.287 ± 0.038
1.906TyrVal: 1.906 ± 0.043
0.403TyrTrp: 0.403 ± 0.019
0.888TyrTyr: 0.888 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4311 proteins (1283078 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski