Candidatus Entotheonella gemina

Taxonomy: cellular organisms; Bacteria; Nitrospinae/Tectomicrobia group; Candidatus Tectomicrobia; Candidatus Entotheonella

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8989 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W4M049|W4M049_9BACT Uncharacterized protein OS=Candidatus Entotheonella gemina OX=1429439 GN=ETSY2_33120 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 10.36RR3 pKa = 11.84LSTLPASCLRR13 pKa = 11.84LMCSIIIGLVSPAWLYY29 pKa = 11.18AQLSLNEE36 pKa = 4.26NATLAQIANEE46 pKa = 4.07LEE48 pKa = 4.48GPGITIFNLSIIVGNTNQYY67 pKa = 8.69GTFTGGTIPSGAGPVVGIDD86 pKa = 3.01TGVFLTTGAVNTIAPDD102 pKa = 3.47RR103 pKa = 11.84ANITPGINDD112 pKa = 3.73NATLGPNTAGGISFSHH128 pKa = 7.29PGPPYY133 pKa = 10.4SDD135 pKa = 4.89PDD137 pKa = 3.49LTSIDD142 pKa = 3.65TQATRR147 pKa = 11.84DD148 pKa = 3.53AMIVEE153 pKa = 4.72FNVVPQQNVLKK164 pKa = 10.05IAFVFGSDD172 pKa = 3.52EE173 pKa = 4.1YY174 pKa = 10.79PEE176 pKa = 4.18YY177 pKa = 11.3VCTIFNDD184 pKa = 3.25AFGFFVTGDD193 pKa = 3.83FGSGTDD199 pKa = 3.33TTRR202 pKa = 11.84NLAVVPGTSVPIAVNTINNGSVGSAQSPGNAAPCDD237 pKa = 3.87LSNSGSFIDD246 pKa = 4.82NGDD249 pKa = 3.55GTTSSLNQNLQLDD262 pKa = 4.71GFTIPLLTQTDD273 pKa = 4.44VIPGNTYY280 pKa = 9.79RR281 pKa = 11.84VKK283 pKa = 10.77LAIADD288 pKa = 3.91ARR290 pKa = 11.84DD291 pKa = 3.86KK292 pKa = 11.17QWDD295 pKa = 3.55SAVFVNFLTSTLFNDD310 pKa = 4.6DD311 pKa = 3.1ADD313 pKa = 4.29LRR315 pKa = 11.84LSKK318 pKa = 10.66QADD321 pKa = 3.59NLAPAVGSNVTFTLTVDD338 pKa = 3.32NDD340 pKa = 4.17GPDD343 pKa = 3.24AAPGVEE349 pKa = 4.39VTDD352 pKa = 4.59LLPSGFTYY360 pKa = 10.8VSDD363 pKa = 4.06SLGGAAGGPFVDD375 pKa = 4.53YY376 pKa = 11.35NPTTGVWTLPSSVASGASATLQITATINASGDD408 pKa = 3.47YY409 pKa = 10.57TNIAEE414 pKa = 4.18ITAAQATDD422 pKa = 4.0PDD424 pKa = 4.35SEE426 pKa = 4.36PDD428 pKa = 3.59NRR430 pKa = 11.84SLNPNEE436 pKa = 5.5DD437 pKa = 3.49DD438 pKa = 3.75TASITLPPVDD448 pKa = 5.45LDD450 pKa = 3.97YY451 pKa = 11.94GDD453 pKa = 5.58APDD456 pKa = 5.06SYY458 pKa = 10.92GTDD461 pKa = 3.48STDD464 pKa = 3.03SSGEE468 pKa = 4.09GVGARR473 pKa = 11.84HH474 pKa = 7.19IINSSLYY481 pKa = 10.45LGAAVPDD488 pKa = 3.5IDD490 pKa = 4.59INGFVDD496 pKa = 6.13GIDD499 pKa = 3.79DD500 pKa = 4.27NGNGTDD506 pKa = 4.68DD507 pKa = 4.94DD508 pKa = 4.17ATGAPGNGDD517 pKa = 4.11DD518 pKa = 4.72EE519 pKa = 5.86DD520 pKa = 6.27SITSFPPLSILAAGYY535 pKa = 7.16TISGIPLHH543 pKa = 6.47NSTRR547 pKa = 11.84STAHH551 pKa = 5.33LVGWIDD557 pKa = 4.06FDD559 pKa = 4.25QSGTFDD565 pKa = 3.9ADD567 pKa = 3.15EE568 pKa = 4.48AATVSVPDD576 pKa = 3.58RR577 pKa = 11.84TSSVTLTWSSLPGIVAGVTYY597 pKa = 10.89ARR599 pKa = 11.84FRR601 pKa = 11.84LTTDD605 pKa = 3.22TSIATGTAATSVPTGTAIDD624 pKa = 4.56GEE626 pKa = 4.55VEE628 pKa = 4.26DD629 pKa = 4.17YY630 pKa = 11.15QLIIGGLTVSGTVYY644 pKa = 11.13NDD646 pKa = 3.42ANHH649 pKa = 6.04NRR651 pKa = 11.84QLDD654 pKa = 3.7SGEE657 pKa = 4.28TGTGLTLFAKK667 pKa = 9.53LIPASTPAGPALSAVPVDD685 pKa = 4.16AGSGAYY691 pKa = 10.17AFTSVNPLTYY701 pKa = 10.08RR702 pKa = 11.84VIIDD706 pKa = 4.13DD707 pKa = 4.04NATLSDD713 pKa = 3.85VTPTLPAGWIGTEE726 pKa = 3.58IPGQIRR732 pKa = 11.84PAVTVVNASVPNQNFGLFNGSQLSGTVFIDD762 pKa = 3.1NGADD766 pKa = 3.05RR767 pKa = 11.84GTPNNGLQDD776 pKa = 3.6GGEE779 pKa = 4.19TGLGSVTVTATDD791 pKa = 3.91GASTVYY797 pKa = 9.74DD798 pKa = 3.86TTTTAADD805 pKa = 3.72GTYY808 pKa = 9.93TLYY811 pKa = 11.07VPAAATTVAVTQTNLSAYY829 pKa = 9.33LSTGGSPGTTGGTYY843 pKa = 10.46DD844 pKa = 4.19RR845 pKa = 11.84NPDD848 pKa = 3.87TVSLTATAGTSYY860 pKa = 10.85SGVDD864 pKa = 4.38FGDD867 pKa = 3.58VPNNTFVPDD876 pKa = 4.12GQQNGLPGTVLWYY889 pKa = 9.5PHH891 pKa = 6.41TFTAGSAGTVTFSTSGSSSPAIPGWSEE918 pKa = 3.91TLYY921 pKa = 10.91QDD923 pKa = 3.99NNCNSIIDD931 pKa = 3.86NGEE934 pKa = 3.86GQIGSIALSAGDD946 pKa = 3.57QVCILMRR953 pKa = 11.84EE954 pKa = 5.06AIPHH958 pKa = 6.61AAPDD962 pKa = 3.57GATRR966 pKa = 11.84QVTVSASFSYY976 pKa = 11.34DD977 pKa = 2.99NASPSLSHH985 pKa = 5.69VLQRR989 pKa = 11.84IDD991 pKa = 3.48LTTVGDD997 pKa = 3.85STSAGLHH1004 pKa = 5.82LLKK1007 pKa = 10.85AVDD1010 pKa = 3.78KK1011 pKa = 8.98TQAAPGEE1018 pKa = 4.33VLTYY1022 pKa = 10.18TITYY1026 pKa = 9.37SNRR1029 pKa = 11.84SNEE1032 pKa = 4.12SLSNIVIHH1040 pKa = 7.22DD1041 pKa = 4.24LTPAFTVFQSATCDD1055 pKa = 3.28LPLPLDD1061 pKa = 3.37ISGCNVTQQPVINGTGAIEE1080 pKa = 3.99WTLGGTLRR1088 pKa = 11.84PGHH1091 pKa = 6.06QGTVTFSIQIRR1102 pKa = 11.84QQ1103 pKa = 3.21

Molecular weight: 113.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W4LQR2|W4LQR2_9BACT Translation initiation factor IF-3 OS=Candidatus Entotheonella gemina OX=1429439 GN=infC PE=3 SV=1
MM1 pKa = 7.89PKK3 pKa = 9.71MKK5 pKa = 9.95TNRR8 pKa = 11.84SAAKK12 pKa = 10.05RR13 pKa = 11.84FRR15 pKa = 11.84LTARR19 pKa = 11.84GKK21 pKa = 9.33VRR23 pKa = 11.84RR24 pKa = 11.84NQAFTRR30 pKa = 11.84HH31 pKa = 5.85ILTKK35 pKa = 9.37KK36 pKa = 6.62TRR38 pKa = 11.84KK39 pKa = 9.41RR40 pKa = 11.84KK41 pKa = 8.77RR42 pKa = 11.84QLRR45 pKa = 11.84PTALVAAADD54 pKa = 3.61APRR57 pKa = 11.84IKK59 pKa = 10.42RR60 pKa = 11.84IITRR64 pKa = 3.66

Molecular weight: 7.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8989

0

8989

2328076

29

2567

259.0

28.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.011 ± 0.031

1.08 ± 0.009

5.677 ± 0.023

6.135 ± 0.026

3.67 ± 0.017

7.572 ± 0.026

2.748 ± 0.015

5.319 ± 0.022

2.981 ± 0.022

10.363 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.593 ± 0.015

2.786 ± 0.015

5.296 ± 0.019

4.501 ± 0.023

6.83 ± 0.021

5.334 ± 0.017

5.541 ± 0.019

7.146 ± 0.022

1.508 ± 0.012

2.911 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski