Ideonella sp. MAG2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Ideonella; unclassified Ideonella

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3953 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A523Q3D7|A0A523Q3D7_9BURK Type I restriction enzyme R Protein OS=Ideonella sp. MAG2 OX=2083017 GN=C4K60_15055 PE=3 SV=1
MM1 pKa = 7.37SLNYY5 pKa = 10.64LHH7 pKa = 7.32FDD9 pKa = 3.55ASDD12 pKa = 3.61DD13 pKa = 4.34GEE15 pKa = 4.6GHH17 pKa = 5.54GTWDD21 pKa = 3.22AMASVKK27 pKa = 10.13PPHH30 pKa = 6.58LPAVLAEE37 pKa = 4.26VQAVLAWCQAHH48 pKa = 6.93APGPCGPLDD57 pKa = 3.92EE58 pKa = 5.4GGAWDD63 pKa = 4.76VEE65 pKa = 4.38QQQTQDD71 pKa = 3.68GDD73 pKa = 3.76WVTVTLTLTGPEE85 pKa = 3.67DD86 pKa = 3.26WGRR89 pKa = 11.84EE90 pKa = 3.76MLAALGADD98 pKa = 3.63AAASS102 pKa = 3.53

Molecular weight: 10.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A523Q3H3|A0A523Q3H3_9BURK ABC transporter substrate-binding protein OS=Ideonella sp. MAG2 OX=2083017 GN=C4K60_15250 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 8.87VRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.65GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.02GRR39 pKa = 11.84KK40 pKa = 8.98KK41 pKa = 10.48LSQVV45 pKa = 2.98

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3953

0

3953

1228316

19

2167

310.7

33.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.856 ± 0.059

0.988 ± 0.013

5.026 ± 0.027

5.167 ± 0.035

3.291 ± 0.026

8.134 ± 0.043

2.407 ± 0.023

3.751 ± 0.031

3.268 ± 0.034

11.115 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.447 ± 0.021

2.528 ± 0.028

5.425 ± 0.031

4.633 ± 0.03

6.675 ± 0.041

5.731 ± 0.037

5.189 ± 0.036

7.532 ± 0.035

1.746 ± 0.023

2.088 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski