Capybara microvirus Cap1_SP_151
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVQ1|A0A4V1FVQ1_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_151 OX=2585393 PE=4 SV=1
MM1 pKa = 7.66 IRR3 pKa = 11.84 RR4 pKa = 11.84 FWMRR8 pKa = 11.84 KK9 pKa = 6.7 EE10 pKa = 4.84 LIDD13 pKa = 5.4 SVEE16 pKa = 4.13 TDD18 pKa = 2.82 QNTAFRR24 pKa = 11.84 IKK26 pKa = 9.78 TKK28 pKa = 10.59 FNFIVYY34 pKa = 9.02 PSNSGSKK41 pKa = 9.32 FHH43 pKa = 6.59 TKK45 pKa = 10.37 YY46 pKa = 10.37 EE47 pKa = 4.23 YY48 pKa = 9.41 KK49 pKa = 7.74 TTQGITEE56 pKa = 4.5 LVPTEE61 pKa = 4.02 EE62 pKa = 4.19 VNTYY66 pKa = 11.16 NEE68 pKa = 4.04 VQSYY72 pKa = 10.02 KK73 pKa = 10.75 EE74 pKa = 3.65 EE75 pKa = 3.87 TSIYY79 pKa = 9.98 KK80 pKa = 10.12 ILDD83 pKa = 2.96 RR84 pKa = 11.84 MTRR87 pKa = 11.84 GDD89 pKa = 4.03 YY90 pKa = 10.75 SALDD94 pKa = 3.63 KK95 pKa = 11.39 NQGIFMDD102 pKa = 4.66 CTNLPKK108 pKa = 10.49 DD109 pKa = 3.61 INEE112 pKa = 4.1 LTDD115 pKa = 3.46 FFNNYY120 pKa = 9.11 EE121 pKa = 4.28 STFNQADD128 pKa = 3.53 PRR130 pKa = 11.84 FKK132 pKa = 10.97 EE133 pKa = 4.17 LFNNNYY139 pKa = 8.95 KK140 pKa = 10.17 QLIFDD145 pKa = 4.36 FQNGTFEE152 pKa = 4.48 EE153 pKa = 5.09 KK154 pKa = 9.65 YY155 pKa = 10.36 KK156 pKa = 10.74 KK157 pKa = 10.53 YY158 pKa = 10.84 NEE160 pKa = 4.4 LYY162 pKa = 10.68 SPEE165 pKa = 4.15 KK166 pKa = 10.11 LQSNNVVSSEE176 pKa = 4.32 PINFEE181 pKa = 3.92 NKK183 pKa = 8.97 EE184 pKa = 4.14 VNDD187 pKa = 3.78 NGG189 pKa = 3.92
Molecular weight: 22.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.968
IPC2_protein 5.067
IPC_protein 4.94
Toseland 4.813
ProMoST 5.029
Dawson 4.876
Bjellqvist 5.016
Wikipedia 4.711
Rodwell 4.787
Grimsley 4.724
Solomon 4.876
Lehninger 4.825
Nozaki 4.978
DTASelect 5.092
Thurlkill 4.8
EMBOSS 4.736
Sillero 5.054
Patrickios 3.999
IPC_peptide 4.889
IPC2_peptide 5.054
IPC2.peptide.svr19 5.059
Protein with the highest isoelectric point:
>tr|A0A4P8W590|A0A4P8W590_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_151 OX=2585393 PE=4 SV=1
MM1 pKa = 7.1 QCFDD5 pKa = 4.5 PFVVSNIRR13 pKa = 11.84 NPLIQLNYY21 pKa = 9.19 KK22 pKa = 9.93 ASKK25 pKa = 9.67 KK26 pKa = 10.13 RR27 pKa = 11.84 RR28 pKa = 11.84 FAYY31 pKa = 9.11 WIKK34 pKa = 10.75 FKK36 pKa = 10.72 DD37 pKa = 3.28 IRR39 pKa = 11.84 KK40 pKa = 9.47 DD41 pKa = 3.38 LLPEE45 pKa = 3.59 VLEE48 pKa = 4.54 RR49 pKa = 11.84 IKK51 pKa = 11.26 NKK53 pKa = 10.58 DD54 pKa = 3.32 MLLIPCGRR62 pKa = 11.84 CFSCRR67 pKa = 11.84 SKK69 pKa = 11.14 KK70 pKa = 10.45 AMSWTIRR77 pKa = 11.84 NYY79 pKa = 10.29 FEE81 pKa = 4.71 SEE83 pKa = 4.28 KK84 pKa = 10.71 YY85 pKa = 9.82 DD86 pKa = 3.34 QKK88 pKa = 11.52 CFITATYY95 pKa = 11.16 NEE97 pKa = 4.63 FKK99 pKa = 10.74 CPRR102 pKa = 11.84 FLVKK106 pKa = 10.1 KK107 pKa = 10.28 DD108 pKa = 3.42 LQLLIKK114 pKa = 9.82 QIRR117 pKa = 11.84 RR118 pKa = 11.84 YY119 pKa = 9.3 IDD121 pKa = 3.02 YY122 pKa = 10.35 HH123 pKa = 8.03 ISSDD127 pKa = 3.45 MKK129 pKa = 10.42 IKK131 pKa = 10.8 YY132 pKa = 7.35 FACGEE137 pKa = 4.04 YY138 pKa = 10.37 GGQFGRR144 pKa = 11.84 PHH146 pKa = 5.83 FHH148 pKa = 6.36 ILLYY152 pKa = 10.19 GFCFPKK158 pKa = 10.7 DD159 pKa = 3.69 HH160 pKa = 6.93 ILKK163 pKa = 10.59 DD164 pKa = 3.73 EE165 pKa = 4.39 NGKK168 pKa = 9.79 KK169 pKa = 10.09 EE170 pKa = 4.15 YY171 pKa = 10.1 ISLKK175 pKa = 9.74 LTEE178 pKa = 4.18 LWNRR182 pKa = 11.84 GIISFSDD189 pKa = 3.86 CVDD192 pKa = 3.3 ASHH195 pKa = 7.42 IKK197 pKa = 8.0 YY198 pKa = 8.01 TCGYY202 pKa = 7.17 VQKK205 pKa = 10.43 KK206 pKa = 9.68 LFHH209 pKa = 6.9 IYY211 pKa = 10.58 SDD213 pKa = 3.92 AEE215 pKa = 4.18 KK216 pKa = 10.89 KK217 pKa = 10.55 LLQDD221 pKa = 4.15 WIEE224 pKa = 4.04 LHH226 pKa = 6.96 PDD228 pKa = 3.01 DD229 pKa = 5.63 HH230 pKa = 6.91 FQDD233 pKa = 4.29 SFITCSKK240 pKa = 10.86 GIGLEE245 pKa = 4.04 FFDD248 pKa = 3.75 KK249 pKa = 10.97 NKK251 pKa = 10.66 YY252 pKa = 9.92 NIYY255 pKa = 10.43 NDD257 pKa = 3.48 NEE259 pKa = 4.36 FLFHH263 pKa = 7.15 PPFDD267 pKa = 4.12 SSPVKK272 pKa = 10.06 HH273 pKa = 6.22 VKK275 pKa = 9.34 PLPYY279 pKa = 9.47 FDD281 pKa = 5.44 YY282 pKa = 11.14 RR283 pKa = 11.84 MQNVDD288 pKa = 3.52 YY289 pKa = 11.01 NLYY292 pKa = 10.3 AHH294 pKa = 6.78 SKK296 pKa = 9.58 FLRR299 pKa = 11.84 SEE301 pKa = 4.12 YY302 pKa = 10.75 NEE304 pKa = 3.84 NYY306 pKa = 9.51 YY307 pKa = 11.04 KK308 pKa = 10.81 KK309 pKa = 10.35 FLEE312 pKa = 4.37 DD313 pKa = 3.58 DD314 pKa = 3.73 RR315 pKa = 11.84 SISEE319 pKa = 3.81 IMRR322 pKa = 11.84 AEE324 pKa = 3.76 EE325 pKa = 5.29 DD326 pKa = 3.88 YY327 pKa = 11.58 ALKK330 pKa = 10.56 NYY332 pKa = 10.37 KK333 pKa = 9.73 LYY335 pKa = 10.84 KK336 pKa = 9.06 YY337 pKa = 10.08 IRR339 pKa = 11.84 RR340 pKa = 3.86
Molecular weight: 41.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.398
IPC2_protein 8.361
IPC_protein 8.2
Toseland 8.58
ProMoST 8.785
Dawson 9.077
Bjellqvist 9.077
Wikipedia 9.194
Rodwell 9.194
Grimsley 8.946
Solomon 9.121
Lehninger 9.092
Nozaki 9.165
DTASelect 8.931
Thurlkill 9.004
EMBOSS 9.209
Sillero 9.224
Patrickios 4.139
IPC_peptide 9.121
IPC2_peptide 7.834
IPC2.peptide.svr19 7.753
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1440
189
560
360.0
41.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.25 ± 1.598
1.667 ± 0.451
6.25 ± 0.354
4.653 ± 1.205
5.903 ± 0.865
5.139 ± 0.86
2.014 ± 0.469
6.667 ± 0.35
7.083 ± 1.569
7.153 ± 0.419
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.639 ± 0.336
7.222 ± 0.899
4.375 ± 0.797
3.611 ± 0.308
3.958 ± 0.443
9.653 ± 1.662
4.653 ± 0.874
4.167 ± 0.67
0.833 ± 0.173
6.111 ± 0.626
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here