Prevotella oulorum F0390

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; Prevotella oulorum

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2483 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1WAD0|G1WAD0_9BACT Uncharacterized protein OS=Prevotella oulorum F0390 OX=702438 GN=HMPREF9431_00775 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 10.29FSIVFMIIVILYY14 pKa = 7.78ILYY17 pKa = 8.84YY18 pKa = 10.0ISMIARR24 pKa = 11.84DD25 pKa = 3.75LYY27 pKa = 11.18FNNKK31 pKa = 7.68EE32 pKa = 4.12VADD35 pKa = 3.9IGAMEE40 pKa = 4.89EE41 pKa = 4.14DD42 pKa = 4.7EE43 pKa = 4.98IDD45 pKa = 3.28ISEE48 pKa = 4.42DD49 pKa = 2.87ARR51 pKa = 11.84NFQTIDD57 pKa = 3.16IPSPNAEE64 pKa = 4.44DD65 pKa = 3.99NEE67 pKa = 4.51IPANTHH73 pKa = 3.8STEE76 pKa = 3.93NNEE79 pKa = 4.29EE80 pKa = 3.89GGIDD84 pKa = 3.47MNGGYY89 pKa = 10.7NIDD92 pKa = 3.71QFIAAANDD100 pKa = 3.51YY101 pKa = 11.12AEE103 pKa = 4.34NGEE106 pKa = 4.15MGEE109 pKa = 4.09AAEE112 pKa = 4.46VCNKK116 pKa = 8.2WVMSVV121 pKa = 2.88

Molecular weight: 13.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1W9F7|G1W9F7_9BACT Serine hydroxymethyltransferase OS=Prevotella oulorum F0390 OX=702438 GN=glyA PE=3 SV=1
MM1 pKa = 7.85PNGKK5 pKa = 9.19KK6 pKa = 10.25KK7 pKa = 10.12KK8 pKa = 7.0GHH10 pKa = 6.14KK11 pKa = 9.06MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2483

0

2483

812405

29

2490

327.2

36.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.095 ± 0.052

1.308 ± 0.021

5.335 ± 0.034

5.528 ± 0.056

4.539 ± 0.029

6.725 ± 0.053

2.444 ± 0.028

6.221 ± 0.054

6.2 ± 0.045

9.326 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.767 ± 0.022

4.958 ± 0.049

3.849 ± 0.027

4.286 ± 0.035

4.88 ± 0.037

5.652 ± 0.039

5.901 ± 0.044

6.428 ± 0.039

1.299 ± 0.021

4.258 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski