Amino acid dipepetide frequency for Chlorella variabilis (Green alga)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	38.699AlaAla: 38.699 ± 0.341	2.359AlaCys: 2.359 ± 0.031	6.053AlaAsp: 6.053 ± 0.043	8.587AlaGlu: 8.587 ± 0.083	3.323AlaPhe: 3.323 ± 0.033	14.482AlaGly: 14.482 ± 0.09	2.392AlaHis: 2.392 ± 0.023	2.98AlaIle: 2.98 ± 0.028	3.625AlaLys: 3.625 ± 0.042	12.529AlaLeu: 12.529 ± 0.087	2.58AlaMet: 2.58 ± 0.027	2.15AlaAsn: 2.15 ± 0.022	8.913AlaPro: 8.913 ± 0.074	6.287AlaGln: 6.287 ± 0.057	7.504AlaArg: 7.504 ± 0.055	9.174AlaSer: 9.174 ± 0.057	6.022AlaThr: 6.022 ± 0.046	9.651AlaVal: 9.651 ± 0.058	1.743AlaTrp: 1.743 ± 0.021	2.161AlaTyr: 2.161 ± 0.025	0.0AlaXaa: 0.0 ± 0.0
Cys	1.753CysAla: 1.753 ± 0.025	0.567CysCys: 0.567 ± 0.014	0.752CysAsp: 0.752 ± 0.014	0.734CysGlu: 0.734 ± 0.013	0.536CysPhe: 0.536 ± 0.012	1.592CysGly: 1.592 ± 0.025	0.416CysHis: 0.416 ± 0.01	0.555CysIle: 0.555 ± 0.012	0.578CysLys: 0.578 ± 0.016	1.766CysLeu: 1.766 ± 0.024	0.382CysMet: 0.382 ± 0.01	0.431CysAsn: 0.431 ± 0.011	1.086CysPro: 1.086 ± 0.02	0.744CysGln: 0.744 ± 0.014	1.31CysArg: 1.31 ± 0.02	1.362CysSer: 1.362 ± 0.021	1.001CysThr: 1.001 ± 0.018	1.065CysVal: 1.065 ± 0.016	0.34CysTrp: 0.34 ± 0.009	0.388CysTyr: 0.388 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	5.93AspAla: 5.93 ± 0.044	0.787AspCys: 0.787 ± 0.014	2.414AspAsp: 2.414 ± 0.03	2.998AspGlu: 2.998 ± 0.033	1.458AspPhe: 1.458 ± 0.018	4.427AspGly: 4.427 ± 0.038	0.74AspHis: 0.74 ± 0.013	1.494AspIle: 1.494 ± 0.022	1.469AspLys: 1.469 ± 0.019	4.138AspLeu: 4.138 ± 0.033	1.006AspMet: 1.006 ± 0.017	0.929AspAsn: 0.929 ± 0.014	2.655AspPro: 2.655 ± 0.026	1.39AspGln: 1.39 ± 0.018	2.424AspArg: 2.424 ± 0.023	2.859AspSer: 2.859 ± 0.03	1.903AspThr: 1.903 ± 0.024	3.269AspVal: 3.269 ± 0.03	0.783AspTrp: 0.783 ± 0.014	1.013AspTyr: 1.013 ± 0.016	0.0AspXaa: 0.0 ± 0.0
Glu	9.478GluAla: 9.478 ± 0.087	0.774GluCys: 0.774 ± 0.015	2.781GluAsp: 2.781 ± 0.033	6.431GluGlu: 6.431 ± 0.088	1.338GluPhe: 1.338 ± 0.021	5.204GluGly: 5.204 ± 0.037	1.189GluHis: 1.189 ± 0.017	1.248GluIle: 1.248 ± 0.022	1.687GluLys: 1.687 ± 0.029	6.083GluLeu: 6.083 ± 0.043	1.154GluMet: 1.154 ± 0.018	0.861GluAsn: 0.861 ± 0.018	2.68GluPro: 2.68 ± 0.028	4.0GluGln: 4.0 ± 0.047	4.203GluArg: 4.203 ± 0.046	2.295GluSer: 2.295 ± 0.025	1.657GluThr: 1.657 ± 0.021	4.166GluVal: 4.166 ± 0.031	0.788GluTrp: 0.788 ± 0.013	1.067GluTyr: 1.067 ± 0.017	0.0GluXaa: 0.0 ± 0.0
Phe	2.813PheAla: 2.813 ± 0.029	0.568PheCys: 0.568 ± 0.013	1.524PheAsp: 1.524 ± 0.019	1.5PheGlu: 1.5 ± 0.016	1.075PhePhe: 1.075 ± 0.019	2.43PheGly: 2.43 ± 0.032	0.601PheHis: 0.601 ± 0.013	0.889PheIle: 0.889 ± 0.015	1.015PheLys: 1.015 ± 0.018	2.666PheLeu: 2.666 ± 0.031	0.64PheMet: 0.64 ± 0.013	0.809PheAsn: 0.809 ± 0.013	1.327PhePro: 1.327 ± 0.019	1.115PheGln: 1.115 ± 0.017	1.58PheArg: 1.58 ± 0.019	1.899PheSer: 1.899 ± 0.022	1.398PheThr: 1.398 ± 0.019	1.934PheVal: 1.934 ± 0.024	0.443PheTrp: 0.443 ± 0.01	0.739PheTyr: 0.739 ± 0.012	0.0PheXaa: 0.0 ± 0.0
Gly	11.963GlyAla: 11.963 ± 0.073	1.585GlyCys: 1.585 ± 0.023	3.962GlyAsp: 3.962 ± 0.034	4.967GlyGlu: 4.967 ± 0.042	2.316GlyPhe: 2.316 ± 0.026	14.13GlyGly: 14.13 ± 0.153	1.714GlyHis: 1.714 ± 0.022	2.375GlyIle: 2.375 ± 0.028	3.043GlyLys: 3.043 ± 0.032	7.469GlyLeu: 7.469 ± 0.05	2.005GlyMet: 2.005 ± 0.029	1.708GlyAsn: 1.708 ± 0.025	4.287GlyPro: 4.287 ± 0.045	3.832GlyGln: 3.832 ± 0.036	6.005GlyArg: 6.005 ± 0.053	7.954GlySer: 7.954 ± 0.057	4.246GlyThr: 4.246 ± 0.036	5.416GlyVal: 5.416 ± 0.04	1.471GlyTrp: 1.471 ± 0.021	1.765GlyTyr: 1.765 ± 0.021	0.0GlyXaa: 0.0 ± 0.0
His	2.654HisAla: 2.654 ± 0.026	0.446HisCys: 0.446 ± 0.011	0.888HisAsp: 0.888 ± 0.015	1.007HisGlu: 1.007 ± 0.016	0.692HisPhe: 0.692 ± 0.016	1.84HisGly: 1.84 ± 0.02	0.773HisHis: 0.773 ± 0.016	0.714HisIle: 0.714 ± 0.014	0.683HisLys: 0.683 ± 0.013	2.316HisLeu: 2.316 ± 0.024	0.524HisMet: 0.524 ± 0.012	0.5HisAsn: 0.5 ± 0.012	1.59HisPro: 1.59 ± 0.02	1.119HisGln: 1.119 ± 0.019	1.575HisArg: 1.575 ± 0.021	1.539HisSer: 1.539 ± 0.02	1.098HisThr: 1.098 ± 0.018	1.385HisVal: 1.385 ± 0.016	0.362HisTrp: 0.362 ± 0.01	0.528HisTyr: 0.528 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	2.804IleAla: 2.804 ± 0.032	0.501IleCys: 0.501 ± 0.013	1.422IleAsp: 1.422 ± 0.02	1.422IleGlu: 1.422 ± 0.018	0.896IlePhe: 0.896 ± 0.016	1.834IleGly: 1.834 ± 0.025	0.578IleHis: 0.578 ± 0.012	0.984IleIle: 0.984 ± 0.019	1.143IleLys: 1.143 ± 0.019	2.294IleLeu: 2.294 ± 0.029	0.613IleMet: 0.613 ± 0.011	0.854IleAsn: 0.854 ± 0.015	1.455IlePro: 1.455 ± 0.02	1.046IleGln: 1.046 ± 0.018	1.531IleArg: 1.531 ± 0.02	1.752IleSer: 1.752 ± 0.024	1.473IleThr: 1.473 ± 0.022	1.834IleVal: 1.834 ± 0.026	0.333IleTrp: 0.333 ± 0.009	0.628IleTyr: 0.628 ± 0.012	0.0IleXaa: 0.0 ± 0.0
Lys	4.023LysAla: 4.023 ± 0.044	0.513LysCys: 0.513 ± 0.016	1.35LysAsp: 1.35 ± 0.019	2.061LysGlu: 2.061 ± 0.031	0.801LysPhe: 0.801 ± 0.015	2.298LysGly: 2.298 ± 0.026	0.709LysHis: 0.709 ± 0.014	0.833LysIle: 0.833 ± 0.017	1.802LysLys: 1.802 ± 0.031	3.154LysLeu: 3.154 ± 0.031	0.65LysMet: 0.65 ± 0.014	0.691LysAsn: 0.691 ± 0.014	1.809LysPro: 1.809 ± 0.025	1.809LysGln: 1.809 ± 0.024	2.402LysArg: 2.402 ± 0.031	1.493LysSer: 1.493 ± 0.021	1.255LysThr: 1.255 ± 0.022	2.044LysVal: 2.044 ± 0.027	0.43LysTrp: 0.43 ± 0.01	0.775LysTyr: 0.775 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	13.779LeuAla: 13.779 ± 0.094	1.676LeuCys: 1.676 ± 0.022	4.572LeuAsp: 4.572 ± 0.035	6.02LeuGlu: 6.02 ± 0.053	2.655LeuPhe: 2.655 ± 0.031	7.411LeuGly: 7.411 ± 0.053	2.649LeuHis: 2.649 ± 0.029	2.188LeuIle: 2.188 ± 0.028	2.958LeuLys: 2.958 ± 0.034	12.135LeuLeu: 12.135 ± 0.102	1.842LeuMet: 1.842 ± 0.023	1.967LeuAsn: 1.967 ± 0.025	7.228LeuPro: 7.228 ± 0.05	5.853LeuGln: 5.853 ± 0.05	7.319LeuArg: 7.319 ± 0.054	6.033LeuSer: 6.033 ± 0.041	4.194LeuThr: 4.194 ± 0.038	6.321LeuVal: 6.321 ± 0.044	1.252LeuTrp: 1.252 ± 0.02	1.933LeuTyr: 1.933 ± 0.025	0.0LeuXaa: 0.0 ± 0.0
Met	2.795MetAla: 2.795 ± 0.025	0.291MetCys: 0.291 ± 0.008	0.92MetAsp: 0.92 ± 0.016	1.175MetGlu: 1.175 ± 0.017	0.53MetPhe: 0.53 ± 0.012	1.531MetGly: 1.531 ± 0.02	0.536MetHis: 0.536 ± 0.011	0.452MetIle: 0.452 ± 0.011	0.588MetLys: 0.588 ± 0.013	2.239MetLeu: 2.239 ± 0.022	0.472MetMet: 0.472 ± 0.01	0.407MetAsn: 0.407 ± 0.01	1.341MetPro: 1.341 ± 0.02	1.271MetGln: 1.271 ± 0.018	1.431MetArg: 1.431 ± 0.018	1.149MetSer: 1.149 ± 0.017	0.758MetThr: 0.758 ± 0.014	1.315MetVal: 1.315 ± 0.019	0.251MetTrp: 0.251 ± 0.007	0.387MetTyr: 0.387 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.11AsnAla: 2.11 ± 0.023	0.404AsnCys: 0.404 ± 0.011	0.818AsnAsp: 0.818 ± 0.014	0.935AsnGlu: 0.935 ± 0.015	0.701AsnPhe: 0.701 ± 0.013	1.801AsnGly: 1.801 ± 0.027	0.394AsnHis: 0.394 ± 0.01	0.818AsnIle: 0.818 ± 0.015	0.806AsnLys: 0.806 ± 0.015	1.965AsnLeu: 1.965 ± 0.028	0.47AsnMet: 0.47 ± 0.011	0.59AsnAsn: 0.59 ± 0.013	1.294AsnPro: 1.294 ± 0.02	0.718AsnGln: 0.718 ± 0.014	1.143AsnArg: 1.143 ± 0.02	1.292AsnSer: 1.292 ± 0.019	1.053AsnThr: 1.053 ± 0.016	1.347AsnVal: 1.347 ± 0.021	0.31AsnTrp: 0.31 ± 0.008	0.516AsnTyr: 0.516 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	10.853ProAla: 10.853 ± 0.092	0.918ProCys: 0.918 ± 0.02	2.752ProAsp: 2.752 ± 0.028	3.452ProGlu: 3.452 ± 0.032	1.518ProPhe: 1.518 ± 0.019	5.942ProGly: 5.942 ± 0.051	1.41ProHis: 1.41 ± 0.019	1.274ProIle: 1.274 ± 0.02	1.578ProLys: 1.578 ± 0.022	5.768ProLeu: 5.768 ± 0.042	0.96ProMet: 0.96 ± 0.016	1.101ProAsn: 1.101 ± 0.014	7.234ProPro: 7.234 ± 0.093	3.149ProGln: 3.149 ± 0.037	3.638ProArg: 3.638 ± 0.031	4.476ProSer: 4.476 ± 0.038	2.778ProThr: 2.778 ± 0.029	3.395ProVal: 3.395 ± 0.034	0.81ProTrp: 0.81 ± 0.015	1.05ProTyr: 1.05 ± 0.016	0.0ProXaa: 0.0 ± 0.0
Gln	7.693GlnAla: 7.693 ± 0.056	0.647GlnCys: 0.647 ± 0.015	1.729GlnAsp: 1.729 ± 0.022	3.303GlnGlu: 3.303 ± 0.044	1.042GlnPhe: 1.042 ± 0.016	3.508GlnGly: 3.508 ± 0.03	1.785GlnHis: 1.785 ± 0.029	0.929GlnIle: 0.929 ± 0.015	1.245GlnLys: 1.245 ± 0.019	6.604GlnLeu: 6.604 ± 0.061	0.896GlnMet: 0.896 ± 0.016	0.662GlnAsn: 0.662 ± 0.013	4.1GlnPro: 4.1 ± 0.047	9.289GlnGln: 9.289 ± 0.161	4.647GlnArg: 4.647 ± 0.046	1.864GlnSer: 1.864 ± 0.022	1.384GlnThr: 1.384 ± 0.017	2.698GlnVal: 2.698 ± 0.024	0.619GlnTrp: 0.619 ± 0.012	0.988GlnTyr: 0.988 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	7.194ArgAla: 7.194 ± 0.049	1.315ArgCys: 1.315 ± 0.021	2.697ArgAsp: 2.697 ± 0.029	3.733ArgGlu: 3.733 ± 0.038	1.833ArgPhe: 1.833 ± 0.022	5.371ArgGly: 5.371 ± 0.051	1.881ArgHis: 1.881 ± 0.023	1.777ArgIle: 1.777 ± 0.022	2.208ArgLys: 2.208 ± 0.025	7.541ArgLeu: 7.541 ± 0.055	1.396ArgMet: 1.396 ± 0.018	1.206ArgAsn: 1.206 ± 0.018	3.886ArgPro: 3.886 ± 0.03	4.738ArgGln: 4.738 ± 0.048	6.788ArgArg: 6.788 ± 0.066	4.123ArgSer: 4.123 ± 0.036	2.488ArgThr: 2.488 ± 0.023	3.897ArgVal: 3.897 ± 0.037	1.15ArgTrp: 1.15 ± 0.016	1.41ArgTyr: 1.41 ± 0.018	0.0ArgXaa: 0.0 ± 0.0
Ser	7.803SerAla: 7.803 ± 0.06	1.184SerCys: 1.184 ± 0.019	2.698SerAsp: 2.698 ± 0.027	2.949SerGlu: 2.949 ± 0.028	1.891SerPhe: 1.891 ± 0.024	6.985SerGly: 6.985 ± 0.053	1.23SerHis: 1.23 ± 0.017	1.821SerIle: 1.821 ± 0.024	2.076SerLys: 2.076 ± 0.024	6.456SerLeu: 6.456 ± 0.053	1.345SerMet: 1.345 ± 0.016	1.402SerAsn: 1.402 ± 0.02	4.343SerPro: 4.343 ± 0.045	2.589SerGln: 2.589 ± 0.027	4.108SerArg: 4.108 ± 0.035	6.532SerSer: 6.532 ± 0.071	3.109SerThr: 3.109 ± 0.028	3.659SerVal: 3.659 ± 0.036	1.03SerTrp: 1.03 ± 0.017	1.275SerTyr: 1.275 ± 0.017	0.0SerXaa: 0.0 ± 0.0
Thr	6.222ThrAla: 6.222 ± 0.049	0.858ThrCys: 0.858 ± 0.019	1.805ThrAsp: 1.805 ± 0.023	1.881ThrGlu: 1.881 ± 0.021	1.399ThrPhe: 1.399 ± 0.023	3.761ThrGly: 3.761 ± 0.032	0.894ThrHis: 0.894 ± 0.014	1.387ThrIle: 1.387 ± 0.019	1.281ThrLys: 1.281 ± 0.018	4.322ThrLeu: 4.322 ± 0.038	0.797ThrMet: 0.797 ± 0.015	0.948ThrAsn: 0.948 ± 0.015	3.145ThrPro: 3.145 ± 0.035	1.615ThrGln: 1.615 ± 0.019	2.275ThrArg: 2.275 ± 0.023	3.019ThrSer: 3.019 ± 0.03	2.198ThrThr: 2.198 ± 0.032	3.033ThrVal: 3.033 ± 0.032	0.623ThrTrp: 0.623 ± 0.013	0.965ThrTyr: 0.965 ± 0.016	0.0ThrXaa: 0.0 ± 0.0
Val	9.154ValAla: 9.154 ± 0.05	1.187ValCys: 1.187 ± 0.017	3.146ValAsp: 3.146 ± 0.03	3.99ValGlu: 3.99 ± 0.035	1.861ValPhe: 1.861 ± 0.021	4.796ValGly: 4.796 ± 0.039	1.463ValHis: 1.463 ± 0.019	1.649ValIle: 1.649 ± 0.022	2.005ValLys: 2.005 ± 0.024	6.955ValLeu: 6.955 ± 0.052	1.335ValMet: 1.335 ± 0.021	1.274ValAsn: 1.274 ± 0.018	4.054ValPro: 4.054 ± 0.036	3.227ValGln: 3.227 ± 0.027	4.027ValArg: 4.027 ± 0.035	3.433ValSer: 3.433 ± 0.03	2.825ValThr: 2.825 ± 0.031	5.234ValVal: 5.234 ± 0.041	0.927ValTrp: 0.927 ± 0.015	1.354ValTyr: 1.354 ± 0.019	0.0ValXaa: 0.0 ± 0.0
Trp	1.517TrpAla: 1.517 ± 0.023	0.283TrpCys: 0.283 ± 0.008	0.721TrpAsp: 0.721 ± 0.014	0.842TrpGlu: 0.842 ± 0.014	0.391TrpPhe: 0.391 ± 0.011	1.133TrpGly: 1.133 ± 0.019	0.406TrpHis: 0.406 ± 0.01	0.336TrpIle: 0.336 ± 0.008	0.416TrpLys: 0.416 ± 0.011	1.606TrpLeu: 1.606 ± 0.019	0.314TrpMet: 0.314 ± 0.008	0.353TrpAsn: 0.353 ± 0.009	0.723TrpPro: 0.723 ± 0.014	0.986TrpGln: 0.986 ± 0.016	1.332TrpArg: 1.332 ± 0.022	0.851TrpSer: 0.851 ± 0.013	0.572TrpThr: 0.572 ± 0.011	0.916TrpVal: 0.916 ± 0.016	0.295TrpTrp: 0.295 ± 0.01	0.295TrpTyr: 0.295 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.976TyrAla: 1.976 ± 0.026	0.494TyrCys: 0.494 ± 0.012	1.083TyrAsp: 1.083 ± 0.016	1.094TyrGlu: 1.094 ± 0.02	0.709TyrPhe: 0.709 ± 0.013	1.738TyrGly: 1.738 ± 0.023	0.471TyrHis: 0.471 ± 0.011	0.694TyrIle: 0.694 ± 0.014	0.696TyrLys: 0.696 ± 0.014	1.979TyrLeu: 1.979 ± 0.023	0.457TyrMet: 0.457 ± 0.01	0.643TyrAsn: 0.643 ± 0.014	0.907TyrPro: 0.907 ± 0.016	0.854TyrGln: 0.854 ± 0.014	1.387TyrArg: 1.387 ± 0.021	1.388TyrSer: 1.388 ± 0.018	1.02TyrThr: 1.02 ± 0.016	1.321TyrVal: 1.321 ± 0.018	0.325TyrTrp: 0.325 ± 0.01	0.584TyrTyr: 0.584 ± 0.013	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 9831 proteins (4464018 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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