Rhizobium sp. Root564

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4726 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q8AQN9|A0A0Q8AQN9_9RHIZ Integrase OS=Rhizobium sp. Root564 OX=1736563 GN=ASD74_20800 PE=4 SV=1
MM1 pKa = 7.95CSTTLLGADD10 pKa = 3.61TTFGGIGLASYY21 pKa = 8.56PNPDD25 pKa = 3.28TFLPIASISPSGLWCTVIVPAVTEE49 pKa = 4.24TDD51 pKa = 3.22TSEE54 pKa = 4.18TPGIRR59 pKa = 11.84LTAVSILLAQDD70 pKa = 3.47AQSIPSTRR78 pKa = 11.84NLVCSSSLMASILLFF93 pKa = 4.17

Molecular weight: 9.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q8B9I6|A0A0Q8B9I6_9RHIZ Uncharacterized protein OS=Rhizobium sp. Root564 OX=1736563 GN=ASD74_19030 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.84LVRR13 pKa = 11.84KK14 pKa = 9.15RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.42GFRR20 pKa = 11.84ARR22 pKa = 11.84MATAGGRR29 pKa = 11.84KK30 pKa = 8.88VLAARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.99

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4726

0

4726

1499049

34

5092

317.2

34.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.391 ± 0.041

0.77 ± 0.012

5.717 ± 0.029

5.826 ± 0.037

3.958 ± 0.023

8.064 ± 0.032

2.002 ± 0.018

5.833 ± 0.028

4.079 ± 0.033

9.908 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.641 ± 0.015

2.981 ± 0.018

4.768 ± 0.03

3.266 ± 0.022

6.381 ± 0.034

6.07 ± 0.025

5.438 ± 0.026

7.321 ± 0.028

1.267 ± 0.015

2.319 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski