Desulfurella amilsii
Average proteome isoelectric point is 7.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2019 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X4XUT4|A0A1X4XUT4_9DELT Uncharacterized protein OS=Desulfurella amilsii OX=1562698 GN=DESAMIL20_843 PE=4 SV=1
MM1 pKa = 7.09 MRR3 pKa = 11.84 SLWSGVSGLQTSQDD17 pKa = 3.36 FMDD20 pKa = 3.58 VVGNNVANVNTVGYY34 pKa = 9.38 KK35 pKa = 10.15 ASQINFSDD43 pKa = 3.73 VLAQTLSGATGPQGALGGMNPMQIGLGTQVASTSKK78 pKa = 10.5 NFSQGSLQTTNVTTNLAIQGNGFFIVSPDD107 pKa = 3.18 NGNTYY112 pKa = 10.66 SYY114 pKa = 10.64 TRR116 pKa = 11.84 AGDD119 pKa = 3.42 FTFDD123 pKa = 3.24 AKK125 pKa = 11.34 GNLVTPTGDD134 pKa = 3.55 VVQGWLANNTTHH146 pKa = 8.07 LINTASTIQGINIPQNMTVPAGVTKK171 pKa = 10.4 NVSMSGNLDD180 pKa = 3.36 GGQTVQTSEE189 pKa = 4.13 LTAITPSLEE198 pKa = 4.01 NSTGAINMNSIYY210 pKa = 10.61 NSNGNLIGVGQTTATAAVGAGSDD233 pKa = 4.03 TVSGLYY239 pKa = 10.12 DD240 pKa = 3.25 INGNQIGSNWTVTVGGTTLTPTPATLSDD268 pKa = 4.26 LANALAPSGTTATITPGGEE287 pKa = 3.81 IQISSTGGTTLPTITSSNPVLNGILSNLSGKK318 pKa = 7.85 TIPAGGTITSLPFFSTPGDD337 pKa = 3.68 TVAMSFDD344 pKa = 3.1 GGANYY349 pKa = 7.76 NTYY352 pKa = 10.15 IYY354 pKa = 10.95 GSGTGGGGTRR364 pKa = 11.84 NSTTGIMSGNVQYY377 pKa = 8.36 FTTLDD382 pKa = 3.77 DD383 pKa = 4.55 LKK385 pKa = 11.43 SDD387 pKa = 3.37 INTDD391 pKa = 3.13 INDD394 pKa = 3.68 NSATPPINNATVTLNSSGEE413 pKa = 4.06 MQIVPTTGSNLSSIGPVYY431 pKa = 10.99 SNNSNLATILGTLSGQVQQTGSTSQPFMADD461 pKa = 3.04 TYY463 pKa = 8.33 STSVAVYY470 pKa = 10.08 DD471 pKa = 4.21 SLGNKK476 pKa = 9.59 HH477 pKa = 6.86 DD478 pKa = 3.63 VTFNFAKK485 pKa = 10.13 TNYY488 pKa = 9.58 NPSTNHH494 pKa = 5.71 TQWEE498 pKa = 4.95 WYY500 pKa = 7.91 ATAPSSGSSTPTLTNASGQITFDD523 pKa = 3.45 STGKK527 pKa = 10.48 LVGLTPPLAITANWNNGTSQQVVNLHH553 pKa = 6.42 FGDD556 pKa = 4.73 LNTFDD561 pKa = 5.51 GLTQFSLPSQTSSLTQDD578 pKa = 3.54 GYY580 pKa = 11.89 AGGSLQNIIVNQNGVISGMFSNGKK604 pKa = 9.72 SYY606 pKa = 11.6 ALGQVSLATFNNNDD620 pKa = 3.31 GLLSEE625 pKa = 4.74 GNSLFEE631 pKa = 4.01 ATANSGTPIVTTAGTGTAGTVIPSEE656 pKa = 4.24 LEE658 pKa = 3.75 EE659 pKa = 4.93 SNVDD663 pKa = 3.88 LGTEE667 pKa = 4.4 FTNMIIAEE675 pKa = 4.11 RR676 pKa = 11.84 AYY678 pKa = 10.04 QANARR683 pKa = 11.84 TLSTSDD689 pKa = 3.18 TMLQSLLNIQQ699 pKa = 3.52
Molecular weight: 71.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.961
IPC_protein 3.986
Toseland 3.757
ProMoST 4.151
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.923
Rodwell 3.808
Grimsley 3.668
Solomon 3.973
Lehninger 3.935
Nozaki 4.088
DTASelect 4.355
Thurlkill 3.808
EMBOSS 3.935
Sillero 4.101
Patrickios 1.278
IPC_peptide 3.961
IPC2_peptide 4.075
IPC2.peptide.svr19 3.974
Protein with the highest isoelectric point:
>tr|A0A1X4XXY5|A0A1X4XXY5_9DELT Z1 domain-containing protein OS=Desulfurella amilsii OX=1562698 GN=DESAMIL20_1953 PE=4 SV=1
MM1 pKa = 7.38 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPHH8 pKa = 4.18 NTPRR12 pKa = 11.84 KK13 pKa = 7.34 RR14 pKa = 11.84 THH16 pKa = 6.22 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 MKK23 pKa = 8.83 TANGRR28 pKa = 11.84 QVLSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.98 GRR39 pKa = 11.84 KK40 pKa = 8.8 RR41 pKa = 11.84 LAII44 pKa = 4.0
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2019
0
2019
603201
37
1518
298.8
33.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.536 ± 0.045
1.071 ± 0.023
5.13 ± 0.04
6.04 ± 0.064
5.371 ± 0.057
5.905 ± 0.05
1.519 ± 0.02
9.505 ± 0.055
9.021 ± 0.06
9.813 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.116 ± 0.02
6.038 ± 0.054
3.295 ± 0.031
3.202 ± 0.039
3.015 ± 0.032
6.534 ± 0.047
4.769 ± 0.043
6.248 ± 0.036
0.698 ± 0.016
4.173 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here