Beihai noda-like virus 11
Average proteome isoelectric point is 7.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFH1|A0A1L3KFH1_9VIRU Capsid protein OS=Beihai noda-like virus 11 OX=1922464 PE=4 SV=1
MM1 pKa = 7.55 AKK3 pKa = 10.23 KK4 pKa = 8.51 PTKK7 pKa = 10.18 KK8 pKa = 9.82 NARR11 pKa = 11.84 NRR13 pKa = 11.84 GSNRR17 pKa = 11.84 PRR19 pKa = 11.84 GAPVAQATNIHH30 pKa = 6.09 VGSRR34 pKa = 11.84 DD35 pKa = 3.35 PPIVVRR41 pKa = 11.84 GTGRR45 pKa = 11.84 FAHH48 pKa = 5.94 STHH51 pKa = 6.74 AANSLSDD58 pKa = 3.5 GDD60 pKa = 4.15 VLFDD64 pKa = 4.98 SIINPSSFPRR74 pKa = 11.84 MSRR77 pKa = 11.84 IASAYY82 pKa = 7.76 QRR84 pKa = 11.84 YY85 pKa = 8.63 RR86 pKa = 11.84 FTRR89 pKa = 11.84 LAFTIQPMCPATTGAGYY106 pKa = 10.56 VAGFLKK112 pKa = 10.79 DD113 pKa = 3.5 PTDD116 pKa = 3.56 EE117 pKa = 4.44 DD118 pKa = 4.19 TSFDD122 pKa = 4.8 AIQGSQGAVIGKK134 pKa = 7.18 WWEE137 pKa = 3.79 HH138 pKa = 4.81 KK139 pKa = 10.11 RR140 pKa = 11.84 IEE142 pKa = 4.31 VRR144 pKa = 11.84 PPTDD148 pKa = 4.24 LLWTSLGEE156 pKa = 4.08 NPRR159 pKa = 11.84 LFSPGKK165 pKa = 9.95 FVMTAVGTNTDD176 pKa = 3.3 AVNVSVLCEE185 pKa = 3.98 WEE187 pKa = 4.21 AVLSVPSLEE196 pKa = 5.08 DD197 pKa = 3.49 YY198 pKa = 10.88 SEE200 pKa = 4.86 KK201 pKa = 10.61 PVTEE205 pKa = 4.09 YY206 pKa = 11.08 FSTKK210 pKa = 10.61 DD211 pKa = 3.82 LLNSSYY217 pKa = 10.31 VHH219 pKa = 6.43 NSNSILQTNVFDD231 pKa = 4.98 GDD233 pKa = 4.08 VIPTGTILRR242 pKa = 11.84 IPFPIDD248 pKa = 2.71 VDD250 pKa = 3.91 YY251 pKa = 11.64 SLGAGDD257 pKa = 3.73 VASARR262 pKa = 11.84 YY263 pKa = 9.45 CYY265 pKa = 10.28 FLAGSHH271 pKa = 5.82 NLTWGLYY278 pKa = 9.67 SGGSFSGGSNYY289 pKa = 10.09 YY290 pKa = 10.63 SDD292 pKa = 4.45 GQPTQCVLPKK302 pKa = 9.0 GTRR305 pKa = 11.84 FEE307 pKa = 4.64 VIPNN311 pKa = 3.7
Molecular weight: 33.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.8
IPC2_protein 6.927
IPC_protein 7.117
Toseland 6.942
ProMoST 7.454
Dawson 7.702
Bjellqvist 7.688
Wikipedia 7.644
Rodwell 7.688
Grimsley 7.015
Solomon 7.819
Lehninger 7.834
Nozaki 7.936
DTASelect 7.834
Thurlkill 7.863
EMBOSS 7.966
Sillero 8.112
Patrickios 4.164
IPC_peptide 7.819
IPC2_peptide 7.307
IPC2.peptide.svr19 7.35
Protein with the highest isoelectric point:
>tr|A0A1L3KFH1|A0A1L3KFH1_9VIRU Capsid protein OS=Beihai noda-like virus 11 OX=1922464 PE=4 SV=1
MM1 pKa = 7.45 SLFHH5 pKa = 7.01 FIASTFRR12 pKa = 11.84 QGVFRR17 pKa = 11.84 MSQSCLLWLITAIASVIGEE36 pKa = 4.31 TLNLPVLIFISLAVFSLSIVAVVKK60 pKa = 9.14 MARR63 pKa = 11.84 SDD65 pKa = 3.09 WFANRR70 pKa = 11.84 LLDD73 pKa = 5.27 LRR75 pKa = 11.84 HH76 pKa = 5.53 QLIGSSVIKK85 pKa = 10.53 IPTASHH91 pKa = 6.48 LRR93 pKa = 11.84 STVQKK98 pKa = 9.68 KK99 pKa = 10.75 LEE101 pKa = 3.9 MTKK104 pKa = 9.92 CGVRR108 pKa = 11.84 RR109 pKa = 11.84 GHH111 pKa = 5.81 SHH113 pKa = 4.72 QQAATEE119 pKa = 4.26 RR120 pKa = 11.84 NSATEE125 pKa = 3.83 TMLDD129 pKa = 3.8 FVTSNGYY136 pKa = 8.87 IPYY139 pKa = 9.8 VISPSPRR146 pKa = 11.84 EE147 pKa = 3.75 AGIDD151 pKa = 3.43 GRR153 pKa = 11.84 RR154 pKa = 11.84 RR155 pKa = 11.84 FYY157 pKa = 11.46 SLADD161 pKa = 3.38 LRR163 pKa = 11.84 QNYY166 pKa = 10.16 LNDD169 pKa = 4.15 PLTDD173 pKa = 3.03 KK174 pKa = 10.92 HH175 pKa = 8.12 IIVMTDD181 pKa = 1.86 VDD183 pKa = 4.78 YY184 pKa = 11.13 YY185 pKa = 11.75 VNMHH189 pKa = 6.02 EE190 pKa = 5.12 LISLGRR196 pKa = 11.84 PILCYY201 pKa = 9.63 TFQPSTVSGPVKK213 pKa = 10.54 DD214 pKa = 4.49 GYY216 pKa = 9.65 FTIKK220 pKa = 10.6 DD221 pKa = 3.92 DD222 pKa = 3.74 VVHH225 pKa = 5.47 YY226 pKa = 10.02 HH227 pKa = 5.72 VNGGKK232 pKa = 9.77 DD233 pKa = 3.32 VQHH236 pKa = 7.07 RR237 pKa = 11.84 IWNYY241 pKa = 9.09 NQDD244 pKa = 3.64 TIYY247 pKa = 11.22 VVDD250 pKa = 4.29 PEE252 pKa = 4.28 NGFWATLRR260 pKa = 11.84 QIIYY264 pKa = 10.55 DD265 pKa = 3.87 LVGLSWLSSICHH277 pKa = 5.62 KK278 pKa = 10.63 RR279 pKa = 11.84 FGIGPFGRR287 pKa = 11.84 KK288 pKa = 7.21 VTICTVDD295 pKa = 3.17 QFKK298 pKa = 11.14 LSDD301 pKa = 3.31 HH302 pKa = 6.63 RR303 pKa = 11.84 NIVSIVPFAKK313 pKa = 10.13 CRR315 pKa = 11.84 EE316 pKa = 3.95 NLLPFEE322 pKa = 5.24 EE323 pKa = 4.8 YY324 pKa = 10.47 GQQLRR329 pKa = 11.84 RR330 pKa = 11.84 MQYY333 pKa = 9.13 GAANPTEE340 pKa = 4.12 QFNTLIYY347 pKa = 10.71 LGDD350 pKa = 4.04 GDD352 pKa = 4.79 PLVSLGVAGQVASVQLPLRR371 pKa = 11.84 DD372 pKa = 4.1 LEE374 pKa = 5.49 AMMLMHH380 pKa = 7.18 NDD382 pKa = 3.05 SKK384 pKa = 10.14 TANLSDD390 pKa = 3.43 TVRR393 pKa = 11.84 RR394 pKa = 11.84 ARR396 pKa = 11.84 ATEE399 pKa = 3.75 KK400 pKa = 10.94 SLTEE404 pKa = 3.59 QQAAIVHH411 pKa = 6.4 HH412 pKa = 6.64 YY413 pKa = 9.11 LLSMLPLKK421 pKa = 10.32 PDD423 pKa = 3.43 VVHH426 pKa = 7.27 KK427 pKa = 10.24 PGQLARR433 pKa = 11.84 HH434 pKa = 5.07 YY435 pKa = 10.45 QATDD439 pKa = 2.74 VKK441 pKa = 10.41 YY442 pKa = 11.02 DD443 pKa = 3.73 HH444 pKa = 7.72 DD445 pKa = 3.84 CTEE448 pKa = 3.69 QGRR451 pKa = 11.84 EE452 pKa = 3.9 YY453 pKa = 11.13 ARR455 pKa = 11.84 TYY457 pKa = 11.39 APGPLSQTAVFPNEE471 pKa = 3.28 SRR473 pKa = 11.84 ANEE476 pKa = 3.93 RR477 pKa = 11.84 ATIDD481 pKa = 3.42 GRR483 pKa = 11.84 VVGPQTKK490 pKa = 10.24 AKK492 pKa = 10.0 ARR494 pKa = 11.84 EE495 pKa = 4.23 NITQRR500 pKa = 11.84 LRR502 pKa = 11.84 KK503 pKa = 8.28 YY504 pKa = 10.69 AKK506 pKa = 10.41 DD507 pKa = 3.46 FVKK510 pKa = 10.62 QLVPNPGAGFRR521 pKa = 11.84 YY522 pKa = 9.35 SVAHH526 pKa = 6.6 VEE528 pKa = 4.23 EE529 pKa = 4.59 QQQKK533 pKa = 8.97 PLQRR537 pKa = 11.84 ARR539 pKa = 11.84 NDD541 pKa = 3.38 ANRR544 pKa = 11.84 MHH546 pKa = 6.8 HH547 pKa = 7.34 AISMITKK554 pKa = 9.73 SFQKK558 pKa = 10.4 KK559 pKa = 7.19 EE560 pKa = 3.8 AYY562 pKa = 8.38 GAPNHH567 pKa = 6.41 PRR569 pKa = 11.84 NISTVPHH576 pKa = 5.77 GQNARR581 pKa = 11.84 LSGYY585 pKa = 8.89 TYY587 pKa = 10.94 AFKK590 pKa = 10.91 DD591 pKa = 3.46 AVLKK595 pKa = 8.91 KK596 pKa = 8.52 TEE598 pKa = 3.81 WYY600 pKa = 8.39 MPCKK604 pKa = 9.91 TPAQIAEE611 pKa = 4.27 AVVNLAAKK619 pKa = 8.93 STEE622 pKa = 4.03 LVEE625 pKa = 4.1 TDD627 pKa = 3.26 YY628 pKa = 11.77 SRR630 pKa = 11.84 FDD632 pKa = 3.3 GTFLRR637 pKa = 11.84 FMRR640 pKa = 11.84 EE641 pKa = 3.82 HH642 pKa = 8.3 VEE644 pKa = 4.0 FACYY648 pKa = 9.55 KK649 pKa = 9.01 RR650 pKa = 11.84 WVPEE654 pKa = 3.63 EE655 pKa = 4.1 LLPEE659 pKa = 4.48 LSEE662 pKa = 4.13 LLNNEE667 pKa = 3.94 VDD669 pKa = 3.64 SKK671 pKa = 11.66 ARR673 pKa = 11.84 TRR675 pKa = 11.84 LGLTYY680 pKa = 10.78 APGCSRR686 pKa = 11.84 LSGSPLTTDD695 pKa = 3.96 GNSICNAFVSYY706 pKa = 10.76 AAGRR710 pKa = 11.84 YY711 pKa = 9.04 SGASHH716 pKa = 6.77 EE717 pKa = 4.65 EE718 pKa = 3.63 AWDD721 pKa = 4.57 DD722 pKa = 3.36 IGIVYY727 pKa = 10.4 GDD729 pKa = 3.96 DD730 pKa = 3.65 GLRR733 pKa = 11.84 SGSVPDD739 pKa = 4.13 EE740 pKa = 4.46 LLSSTASDD748 pKa = 3.8 LGFEE752 pKa = 4.81 LKK754 pKa = 9.92 ICNRR758 pKa = 11.84 ASRR761 pKa = 11.84 GQSVSFLSRR770 pKa = 11.84 IFCDD774 pKa = 3.36 PWSSPASIQDD784 pKa = 3.61 PARR787 pKa = 11.84 TLLKK791 pKa = 10.33 IHH793 pKa = 5.84 TTCDD797 pKa = 3.21 GSNKK801 pKa = 9.29 TIQEE805 pKa = 3.97 IGWAKK810 pKa = 7.63 TQAYY814 pKa = 8.91 LATDD818 pKa = 3.97 RR819 pKa = 11.84 LTPFISDD826 pKa = 2.71 WCLAYY831 pKa = 10.31 QRR833 pKa = 11.84 NCPEE837 pKa = 3.62 QLVNYY842 pKa = 9.98 DD843 pKa = 4.91 DD844 pKa = 4.09 YY845 pKa = 11.8 TDD847 pKa = 3.21 IPFWVRR853 pKa = 11.84 DD854 pKa = 3.56 EE855 pKa = 5.67 DD856 pKa = 4.06 SLNNSWPQSDD866 pKa = 3.63 NEE868 pKa = 3.87 IWVGIVANRR877 pKa = 11.84 LGVTASEE884 pKa = 4.18 LDD886 pKa = 3.34 AHH888 pKa = 6.39 IKK890 pKa = 9.82 KK891 pKa = 10.15 LRR893 pKa = 11.84 AFTGRR898 pKa = 11.84 VEE900 pKa = 4.51 DD901 pKa = 4.34 LPVLTSHH908 pKa = 7.76 IDD910 pKa = 3.32 QTPKK914 pKa = 11.02 LEE916 pKa = 3.91 VAMDD920 pKa = 4.03 GEE922 pKa = 4.56 IHH924 pKa = 7.07 AGPSSVNKK932 pKa = 9.02 KK933 pKa = 8.19 TEE935 pKa = 3.87 NGPNHH940 pKa = 6.53 HH941 pKa = 7.19 GDD943 pKa = 3.73 RR944 pKa = 11.84 EE945 pKa = 4.19 DD946 pKa = 3.55 SGTAEE951 pKa = 4.18 TAVPTSRR958 pKa = 11.84 GSPAKK963 pKa = 9.79 PGRR966 pKa = 11.84 AVPGRR971 pKa = 11.84 GKK973 pKa = 10.39 RR974 pKa = 11.84 DD975 pKa = 3.38 ANVCAEE981 pKa = 4.27 RR982 pKa = 11.84 EE983 pKa = 4.25 RR984 pKa = 11.84 KK985 pKa = 9.58 GGRR988 pKa = 11.84 NQASAIAHH996 pKa = 5.93 SNSASADD1003 pKa = 3.4 KK1004 pKa = 10.19 PHH1006 pKa = 7.06 RR1007 pKa = 11.84 RR1008 pKa = 11.84 RR1009 pKa = 11.84 PTPQVSGPRR1018 pKa = 11.84 KK1019 pKa = 9.2 PKK1021 pKa = 9.94 VV1022 pKa = 3.06
Molecular weight: 114.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.578
IPC2_protein 7.907
IPC_protein 7.717
Toseland 7.497
ProMoST 8.302
Dawson 8.478
Bjellqvist 8.77
Wikipedia 8.419
Rodwell 8.507
Grimsley 7.585
Solomon 8.521
Lehninger 8.521
Nozaki 8.902
DTASelect 8.521
Thurlkill 8.58
EMBOSS 8.668
Sillero 8.873
Patrickios 4.431
IPC_peptide 8.507
IPC2_peptide 7.673
IPC2.peptide.svr19 7.822
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1333
311
1022
666.5
74.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.952 ± 0.268
1.575 ± 0.139
5.851 ± 0.031
4.501 ± 0.62
3.676 ± 0.553
6.827 ± 0.894
2.926 ± 0.48
5.026 ± 0.098
4.501 ± 0.465
7.727 ± 0.625
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.575 ± 0.139
4.576 ± 0.274
5.851 ± 0.589
3.901 ± 0.64
6.827 ± 0.501
8.327 ± 0.791
6.377 ± 0.491
6.977 ± 0.202
1.425 ± 0.088
3.601 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here