Terrapene carolina carolina ranavirus
Average proteome isoelectric point is 7.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U9QGY3|A0A2U9QGY3_FRG3V Uncharacterized protein OS=Terrapene carolina carolina ranavirus OX=2219561 GN=ORF87 PE=4 SV=1
MM1 pKa = 8.17 DD2 pKa = 4.92 KK3 pKa = 9.58 PTVEE7 pKa = 4.01 TSAAPVEE14 pKa = 4.38 TLVLTEE20 pKa = 4.99 PPAEE24 pKa = 4.21 TQAEE28 pKa = 4.21 DD29 pKa = 3.62 SVSSVLAGLTAAIEE43 pKa = 4.33 TVDD46 pKa = 3.64 RR47 pKa = 11.84 LRR49 pKa = 11.84 TAFGAEE55 pKa = 3.81
Molecular weight: 5.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.462
IPC2_protein 4.088
IPC_protein 3.872
Toseland 3.732
ProMoST 3.948
Dawson 3.834
Bjellqvist 4.113
Wikipedia 3.745
Rodwell 3.732
Grimsley 3.656
Solomon 3.808
Lehninger 3.757
Nozaki 3.986
DTASelect 4.05
Thurlkill 3.77
EMBOSS 3.757
Sillero 3.986
Patrickios 3.961
IPC_peptide 3.808
IPC2_peptide 3.973
IPC2.peptide.svr19 3.937
Protein with the highest isoelectric point:
>tr|A0A2U9QGT1|A0A2U9QGT1_FRG3V Uncharacterized protein OS=Terrapene carolina carolina ranavirus OX=2219561 GN=ORF41 PE=4 SV=1
MM1 pKa = 7.11 YY2 pKa = 10.24 SVRR5 pKa = 11.84 NSGCSVGCSPRR16 pKa = 11.84 QGASPIMFGPSLGAMLSAPVVRR38 pKa = 11.84 ASAPVVRR45 pKa = 11.84 ASSPVVKK52 pKa = 10.32 RR53 pKa = 11.84 KK54 pKa = 10.35 SLVKK58 pKa = 10.26 RR59 pKa = 11.84 KK60 pKa = 10.2 SPVKK64 pKa = 10.34 RR65 pKa = 11.84 SPLKK69 pKa = 10.35 KK70 pKa = 8.96 RR71 pKa = 11.84 SQMRR75 pKa = 11.84 TSPVKK80 pKa = 10.29 RR81 pKa = 11.84 KK82 pKa = 10.42 SPVKK86 pKa = 10.07 RR87 pKa = 11.84 KK88 pKa = 10.4 SPVKK92 pKa = 10.07 RR93 pKa = 11.84 KK94 pKa = 10.4 SPVKK98 pKa = 10.07 RR99 pKa = 11.84 KK100 pKa = 10.4 SPVKK104 pKa = 10.07 RR105 pKa = 11.84 KK106 pKa = 10.4 SPVKK110 pKa = 10.07 RR111 pKa = 11.84 KK112 pKa = 10.4 SPVKK116 pKa = 10.07 RR117 pKa = 11.84 KK118 pKa = 10.4 SPVKK122 pKa = 10.07 RR123 pKa = 11.84 KK124 pKa = 10.4 SPVKK128 pKa = 10.07 RR129 pKa = 11.84 KK130 pKa = 10.4 SPVKK134 pKa = 10.07 RR135 pKa = 11.84 KK136 pKa = 10.4 SPVKK140 pKa = 10.05 RR141 pKa = 11.84 KK142 pKa = 10.07 SPAKK146 pKa = 10.0 RR147 pKa = 11.84 RR148 pKa = 11.84 QQVCFMATKK157 pKa = 10.43
Molecular weight: 17.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.386
IPC2_protein 10.935
IPC_protein 12.384
Toseland 12.559
ProMoST 13.027
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.486
Grimsley 12.603
Solomon 13.042
Lehninger 12.939
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.193
IPC_peptide 13.042
IPC2_peptide 12.032
IPC2.peptide.svr19 8.979
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
95
0
95
28294
43
1293
297.8
32.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.726 ± 0.272
2.135 ± 0.141
6.072 ± 0.18
5.598 ± 0.232
3.266 ± 0.123
7.376 ± 0.262
2.202 ± 0.152
3.23 ± 0.131
5.627 ± 0.273
8.002 ± 0.265
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.064 ± 0.145
2.658 ± 0.14
6.432 ± 0.333
2.888 ± 0.162
7.143 ± 0.301
7.206 ± 0.254
5.478 ± 0.199
8.454 ± 0.216
1.46 ± 0.112
2.983 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here