Spiroplasma phage SVGII3

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; unclassified Inoviridae

Average proteome isoelectric point is 7.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q3ZVF2|Q3ZVF2_9VIRU Uncharacterized protein OS=Spiroplasma phage SVGII3 OX=745349 PE=4 SV=1
MM1 pKa = 7.53LEE3 pKa = 4.15KK4 pKa = 10.56SVFDD8 pKa = 3.56IYY10 pKa = 10.89IEE12 pKa = 3.95FMQIIFGVDD21 pKa = 3.31MPPALAYY28 pKa = 9.75ICFTLFLTFIIGVIFWIFRR47 pKa = 11.84LIFRR51 pKa = 11.84GFCC54 pKa = 3.46

Molecular weight: 6.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q3ZVE9|Q3ZVE9_9VIRU Uncharacterized protein OS=Spiroplasma phage SVGII3 OX=745349 PE=4 SV=1
MM1 pKa = 7.74IKK3 pKa = 10.48LLWFFLVIGIIGSAFVANIFLASEE27 pKa = 4.02TAMATIKK34 pKa = 10.3QWFNSSNEE42 pKa = 3.93FYY44 pKa = 11.21KK45 pKa = 10.96LVANLFIIATHH56 pKa = 6.56PIFRR60 pKa = 11.84LFIAFGIMLMIIRR73 pKa = 11.84VIFVVRR79 pKa = 4.07

Molecular weight: 9.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

1750

54

469

159.1

18.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.229 ± 0.528

0.686 ± 0.313

4.457 ± 0.583

4.571 ± 0.537

8.286 ± 1.186

5.029 ± 0.396

1.2 ± 0.255

10.4 ± 1.221

10.057 ± 0.991

9.429 ± 0.877

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.086 ± 0.404

7.771 ± 0.873

2.571 ± 0.275

2.457 ± 0.299

2.857 ± 0.37

5.086 ± 0.578

3.943 ± 0.276

5.771 ± 0.381

2.229 ± 0.336

5.886 ± 0.8
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski