Amino acid dipepetide frequency for Spiroplasma phage SVGII3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.0AlaAla: 0.0 ± 0.0
0.572AlaCys: 0.572 ± 0.773
1.144AlaAsp: 1.144 ± 1.102
1.144AlaGlu: 1.144 ± 0.556
5.146AlaPhe: 5.146 ± 1.723
3.431AlaGly: 3.431 ± 2.234
0.572AlaHis: 0.572 ± 0.428
4.002AlaIle: 4.002 ± 1.683
1.715AlaLys: 1.715 ± 0.706
5.146AlaLeu: 5.146 ± 2.013
2.287AlaMet: 2.287 ± 0.905
5.146AlaAsn: 5.146 ± 1.566
1.144AlaPro: 1.144 ± 0.769
0.0AlaGln: 0.0 ± 0.0
0.572AlaArg: 0.572 ± 0.428
2.287AlaSer: 2.287 ± 1.035
4.002AlaThr: 4.002 ± 1.735
0.572AlaVal: 0.572 ± 0.481
0.0AlaTrp: 0.0 ± 0.0
3.431AlaTyr: 3.431 ± 1.469
0.0AlaXaa: 0.0 ± 0.0
Cys
0.572CysAla: 0.572 ± 0.736
0.0CysCys: 0.0 ± 0.0
0.0CysAsp: 0.0 ± 0.0
0.572CysGlu: 0.572 ± 0.481
0.572CysPhe: 0.572 ± 0.834
0.0CysGly: 0.0 ± 0.0
0.0CysHis: 0.0 ± 0.0
1.144CysIle: 1.144 ± 0.961
1.144CysLys: 1.144 ± 0.809
0.572CysLeu: 0.572 ± 0.462
0.572CysMet: 0.572 ± 0.731
0.0CysAsn: 0.0 ± 0.0
0.0CysPro: 0.0 ± 0.0
0.572CysGln: 0.572 ± 0.481
0.0CysArg: 0.0 ± 0.0
0.0CysSer: 0.0 ± 0.0
0.572CysThr: 0.572 ± 0.481
0.572CysVal: 0.572 ± 0.773
0.0CysTrp: 0.0 ± 0.0
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
1.715AspAla: 1.715 ± 1.022
0.572AspCys: 0.572 ± 0.462
1.144AspAsp: 1.144 ± 0.769
1.144AspGlu: 1.144 ± 0.491
2.287AspPhe: 2.287 ± 1.71
1.715AspGly: 1.715 ± 0.505
0.0AspHis: 0.0 ± 0.0
5.718AspIle: 5.718 ± 1.862
6.289AspLys: 6.289 ± 1.854
3.431AspLeu: 3.431 ± 1.294
3.431AspMet: 3.431 ± 1.042
2.859AspAsn: 2.859 ± 1.251
0.572AspPro: 0.572 ± 0.481
0.572AspGln: 0.572 ± 0.428
0.572AspArg: 0.572 ± 0.462
2.859AspSer: 2.859 ± 1.229
1.144AspThr: 1.144 ± 0.576
4.574AspVal: 4.574 ± 1.476
2.287AspTrp: 2.287 ± 0.986
1.715AspTyr: 1.715 ± 0.505
0.0AspXaa: 0.0 ± 0.0
Glu
0.572GluAla: 0.572 ± 0.773
0.572GluCys: 0.572 ± 0.481
1.144GluAsp: 1.144 ± 0.491
1.144GluGlu: 1.144 ± 0.556
3.431GluPhe: 3.431 ± 1.17
1.715GluGly: 1.715 ± 1.518
0.0GluHis: 0.0 ± 0.0
2.859GluIle: 2.859 ± 1.778
6.289GluLys: 6.289 ± 1.618
3.431GluLeu: 3.431 ± 1.491
2.287GluMet: 2.287 ± 0.989
5.718GluAsn: 5.718 ± 1.476
1.144GluPro: 1.144 ± 0.855
1.715GluGln: 1.715 ± 1.119
0.0GluArg: 0.0 ± 0.0
1.715GluSer: 1.715 ± 1.103
4.002GluThr: 4.002 ± 1.264
3.431GluVal: 3.431 ± 1.086
0.0GluTrp: 0.0 ± 0.0
4.574GluTyr: 4.574 ± 1.281
0.0GluXaa: 0.0 ± 0.0
Phe
0.572PheAla: 0.572 ± 0.428
0.572PheCys: 0.572 ± 0.834
4.574PheAsp: 4.574 ± 1.797
1.715PheGlu: 1.715 ± 1.283
6.861PhePhe: 6.861 ± 1.778
4.574PheGly: 4.574 ± 1.708
0.572PheHis: 0.572 ± 0.481
6.861PheIle: 6.861 ± 1.998
7.433PheLys: 7.433 ± 2.693
9.148PheLeu: 9.148 ± 3.168
3.431PheMet: 3.431 ± 1.278
8.576PheAsn: 8.576 ± 3.04
1.144PhePro: 1.144 ± 0.491
1.144PheGln: 1.144 ± 0.783
2.287PheArg: 2.287 ± 1.783
8.576PheSer: 8.576 ± 3.782
2.859PheThr: 2.859 ± 0.997
6.289PheVal: 6.289 ± 1.331
2.859PheTrp: 2.859 ± 1.216
2.859PheTyr: 2.859 ± 1.383
0.0PheXaa: 0.0 ± 0.0
Gly
4.002GlyAla: 4.002 ± 1.001
0.572GlyCys: 0.572 ± 0.481
1.144GlyAsp: 1.144 ± 0.491
2.287GlyGlu: 2.287 ± 1.065
4.574GlyPhe: 4.574 ± 1.646
2.859GlyGly: 2.859 ± 0.901
0.0GlyHis: 0.0 ± 0.0
9.148GlyIle: 9.148 ± 2.462
5.146GlyLys: 5.146 ± 1.435
1.144GlyLeu: 1.144 ± 1.087
0.572GlyMet: 0.572 ± 0.462
2.287GlyAsn: 2.287 ± 1.25
0.572GlyPro: 0.572 ± 0.481
0.572GlyGln: 0.572 ± 0.462
0.0GlyArg: 0.0 ± 0.0
3.431GlySer: 3.431 ± 1.322
4.002GlyThr: 4.002 ± 1.54
4.002GlyVal: 4.002 ± 1.735
1.715GlyTrp: 1.715 ± 0.769
2.287GlyTyr: 2.287 ± 1.295
0.0GlyXaa: 0.0 ± 0.0
His
0.572HisAla: 0.572 ± 0.773
0.0HisCys: 0.0 ± 0.0
0.0HisAsp: 0.0 ± 0.0
0.0HisGlu: 0.0 ± 0.0
1.144HisPhe: 1.144 ± 0.961
1.144HisGly: 1.144 ± 0.708
0.572HisHis: 0.572 ± 0.462
2.287HisIle: 2.287 ± 0.97
0.0HisLys: 0.0 ± 0.0
0.0HisLeu: 0.0 ± 0.0
0.0HisMet: 0.0 ± 0.0
4.002HisAsn: 4.002 ± 2.023
0.572HisPro: 0.572 ± 0.648
0.572HisGln: 0.572 ± 0.481
0.572HisArg: 0.572 ± 0.754
0.0HisSer: 0.0 ± 0.0
0.0HisThr: 0.0 ± 0.0
0.0HisVal: 0.0 ± 0.0
0.572HisTrp: 0.572 ± 0.462
0.0HisTyr: 0.0 ± 0.0
0.0HisXaa: 0.0 ± 0.0
Ile
4.002IleAla: 4.002 ± 2.042
1.144IleCys: 1.144 ± 1.104
3.431IleAsp: 3.431 ± 1.493
4.002IleGlu: 4.002 ± 1.517
12.579IlePhe: 12.579 ± 4.24
5.718IleGly: 5.718 ± 2.672
3.431IleHis: 3.431 ± 1.602
10.863IleIle: 10.863 ± 3.712
12.579IleLys: 12.579 ± 3.828
7.433IleLeu: 7.433 ± 3.465
2.859IleMet: 2.859 ± 1.88
5.718IleAsn: 5.718 ± 1.298
5.718IlePro: 5.718 ± 1.816
1.144IleGln: 1.144 ± 0.556
5.146IleArg: 5.146 ± 1.255
3.431IleSer: 3.431 ± 1.649
4.002IleThr: 4.002 ± 1.744
5.146IleVal: 5.146 ± 2.222
2.287IleTrp: 2.287 ± 0.981
3.431IleTyr: 3.431 ± 0.997
0.0IleXaa: 0.0 ± 0.0
Lys
4.002LysAla: 4.002 ± 1.859
0.0LysCys: 0.0 ± 0.0
8.576LysAsp: 8.576 ± 2.302
6.289LysGlu: 6.289 ± 3.771
4.002LysPhe: 4.002 ± 0.996
3.431LysGly: 3.431 ± 0.993
2.859LysHis: 2.859 ± 0.818
9.72LysIle: 9.72 ± 2.66
9.148LysLys: 9.148 ± 2.533
7.433LysLeu: 7.433 ± 1.6
2.859LysMet: 2.859 ± 1.005
8.576LysAsn: 8.576 ± 2.571
0.572LysPro: 0.572 ± 0.428
2.859LysGln: 2.859 ± 1.35
1.715LysArg: 1.715 ± 0.881
5.146LysSer: 5.146 ± 1.365
4.002LysThr: 4.002 ± 1.245
5.146LysVal: 5.146 ± 1.823
1.715LysTrp: 1.715 ± 1.013
12.579LysTyr: 12.579 ± 3.486
0.0LysXaa: 0.0 ± 0.0
Leu
6.861LeuAla: 6.861 ± 2.216
0.0LeuCys: 0.0 ± 0.0
4.574LeuAsp: 4.574 ± 1.567
5.146LeuGlu: 5.146 ± 1.094
7.433LeuPhe: 7.433 ± 2.056
5.718LeuGly: 5.718 ± 1.802
0.572LeuHis: 0.572 ± 0.481
10.863LeuIle: 10.863 ± 3.944
7.433LeuLys: 7.433 ± 2.269
6.861LeuLeu: 6.861 ± 1.967
5.146LeuMet: 5.146 ± 1.59
4.002LeuAsn: 4.002 ± 1.292
5.146LeuPro: 5.146 ± 1.365
3.431LeuGln: 3.431 ± 1.184
2.859LeuArg: 2.859 ± 1.197
2.287LeuSer: 2.287 ± 1.142
3.431LeuThr: 3.431 ± 1.031
5.146LeuVal: 5.146 ± 1.769
2.859LeuTrp: 2.859 ± 1.731
4.574LeuTyr: 4.574 ± 1.367
0.0LeuXaa: 0.0 ± 0.0
Met
1.715MetAla: 1.715 ± 0.663
0.572MetCys: 0.572 ± 0.481
2.287MetAsp: 2.287 ± 1.322
2.287MetGlu: 2.287 ± 1.091
3.431MetPhe: 3.431 ± 1.06
1.144MetGly: 1.144 ± 0.576
0.572MetHis: 0.572 ± 0.428
3.431MetIle: 3.431 ± 1.75
1.715MetLys: 1.715 ± 1.113
2.287MetLeu: 2.287 ± 1.061
0.0MetMet: 0.0 ± 0.0
2.287MetAsn: 2.287 ± 1.023
1.144MetPro: 1.144 ± 0.897
0.572MetGln: 0.572 ± 0.834
2.859MetArg: 2.859 ± 1.015
1.715MetSer: 1.715 ± 1.424
0.0MetThr: 0.0 ± 0.0
1.715MetVal: 1.715 ± 1.145
0.572MetTrp: 0.572 ± 0.716
0.572MetTyr: 0.572 ± 0.481
0.0MetXaa: 0.0 ± 0.0
Asn
3.431AsnAla: 3.431 ± 1.58
1.715AsnCys: 1.715 ± 0.955
2.287AsnAsp: 2.287 ± 1.152
3.431AsnGlu: 3.431 ± 0.69
7.433AsnPhe: 7.433 ± 1.817
3.431AsnGly: 3.431 ± 1.615
0.572AsnHis: 0.572 ± 0.481
4.574AsnIle: 4.574 ± 1.675
8.576AsnLys: 8.576 ± 2.619
10.292AsnLeu: 10.292 ± 2.678
0.572AsnMet: 0.572 ± 0.549
6.861AsnAsn: 6.861 ± 1.623
2.287AsnPro: 2.287 ± 0.855
1.715AsnGln: 1.715 ± 0.743
1.715AsnArg: 1.715 ± 1.097
4.002AsnSer: 4.002 ± 2.149
3.431AsnThr: 3.431 ± 1.404
4.574AsnVal: 4.574 ± 1.392
2.859AsnTrp: 2.859 ± 1.027
3.431AsnTyr: 3.431 ± 1.353
0.0AsnXaa: 0.0 ± 0.0
Pro
1.715ProAla: 1.715 ± 1.151
0.0ProCys: 0.0 ± 0.0
0.0ProAsp: 0.0 ± 0.0
0.572ProGlu: 0.572 ± 0.719
0.572ProPhe: 0.572 ± 0.428
1.144ProGly: 1.144 ± 0.855
0.572ProHis: 0.572 ± 0.481
4.574ProIle: 4.574 ± 1.461
2.859ProLys: 2.859 ± 1.095
2.859ProLeu: 2.859 ± 0.843
0.0ProMet: 0.0 ± 0.0
0.572ProAsn: 0.572 ± 0.481
0.572ProPro: 0.572 ± 0.834
0.572ProGln: 0.572 ± 0.716
0.0ProArg: 0.0 ± 0.0
1.144ProSer: 1.144 ± 0.491
0.572ProThr: 0.572 ± 0.481
1.144ProVal: 1.144 ± 0.961
0.572ProTrp: 0.572 ± 0.428
5.718ProTyr: 5.718 ± 2.069
0.0ProXaa: 0.0 ± 0.0
Gln
0.0GlnAla: 0.0 ± 0.0
0.0GlnCys: 0.0 ± 0.0
1.715GlnAsp: 1.715 ± 0.743
0.572GlnGlu: 0.572 ± 0.481
1.715GlnPhe: 1.715 ± 0.927
0.572GlnGly: 0.572 ± 0.428
0.0GlnHis: 0.0 ± 0.0
2.287GlnIle: 2.287 ± 1.331
4.002GlnLys: 4.002 ± 1.051
2.287GlnLeu: 2.287 ± 0.972
0.572GlnMet: 0.572 ± 0.481
3.431GlnAsn: 3.431 ± 1.471
0.572GlnPro: 0.572 ± 0.481
1.144GlnGln: 1.144 ± 0.836
2.287GlnArg: 2.287 ± 1.398
1.715GlnSer: 1.715 ± 0.76
0.0GlnThr: 0.0 ± 0.0
0.572GlnVal: 0.572 ± 0.719
0.572GlnTrp: 0.572 ± 0.648
0.572GlnTyr: 0.572 ± 0.462
0.0GlnXaa: 0.0 ± 0.0
Arg
0.0ArgAla: 0.0 ± 0.0
0.0ArgCys: 0.0 ± 0.0
1.144ArgAsp: 1.144 ± 0.576
1.144ArgGlu: 1.144 ± 0.923
1.715ArgPhe: 1.715 ± 0.948
2.859ArgGly: 2.859 ± 1.171
0.0ArgHis: 0.0 ± 0.0
0.572ArgIle: 0.572 ± 0.481
5.718ArgLys: 5.718 ± 1.898
4.574ArgLeu: 4.574 ± 1.562
0.572ArgMet: 0.572 ± 0.611
1.144ArgAsn: 1.144 ± 0.576
0.572ArgPro: 0.572 ± 0.481
2.287ArgGln: 2.287 ± 1.016
0.0ArgArg: 0.0 ± 0.0
1.715ArgSer: 1.715 ± 0.505
0.0ArgThr: 0.0 ± 0.0
2.287ArgVal: 2.287 ± 1.018
0.0ArgTrp: 0.0 ± 0.0
2.287ArgTyr: 2.287 ± 1.113
0.0ArgXaa: 0.0 ± 0.0
Ser
4.002SerAla: 4.002 ± 1.884
0.0SerCys: 0.0 ± 0.0
1.715SerAsp: 1.715 ± 0.868
5.146SerGlu: 5.146 ± 1.438
2.287SerPhe: 2.287 ± 1.378
1.715SerGly: 1.715 ± 1.385
0.0SerHis: 0.0 ± 0.0
4.574SerIle: 4.574 ± 1.394
3.431SerLys: 3.431 ± 1.526
6.289SerLeu: 6.289 ± 1.657
0.572SerMet: 0.572 ± 0.656
6.289SerAsn: 6.289 ± 1.923
1.144SerPro: 1.144 ± 0.491
2.287SerGln: 2.287 ± 1.064
0.572SerArg: 0.572 ± 0.481
2.287SerSer: 2.287 ± 0.852
4.002SerThr: 4.002 ± 1.654
1.144SerVal: 1.144 ± 0.927
1.144SerTrp: 1.144 ± 0.769
2.287SerTyr: 2.287 ± 1.261
0.0SerXaa: 0.0 ± 0.0
Thr
2.859ThrAla: 2.859 ± 1.077
0.0ThrCys: 0.0 ± 0.0
1.715ThrAsp: 1.715 ± 0.795
2.287ThrGlu: 2.287 ± 1.565
4.574ThrPhe: 4.574 ± 1.277
4.002ThrGly: 4.002 ± 1.859
0.572ThrHis: 0.572 ± 0.648
4.002ThrIle: 4.002 ± 1.024
2.859ThrLys: 2.859 ± 0.995
6.861ThrLeu: 6.861 ± 2.396
1.144ThrMet: 1.144 ± 0.779
1.144ThrAsn: 1.144 ± 0.556
0.572ThrPro: 0.572 ± 0.462
0.0ThrGln: 0.0 ± 0.0
0.572ThrArg: 0.572 ± 0.462
1.144ThrSer: 1.144 ± 1.069
1.715ThrThr: 1.715 ± 1.32
1.144ThrVal: 1.144 ± 0.576
0.572ThrTrp: 0.572 ± 0.665
1.715ThrTyr: 1.715 ± 0.927
0.0ThrXaa: 0.0 ± 0.0
Val
2.859ValAla: 2.859 ± 1.784
0.572ValCys: 0.572 ± 0.719
2.287ValAsp: 2.287 ± 1.314
2.859ValGlu: 2.859 ± 1.83
4.574ValPhe: 4.574 ± 1.507
3.431ValGly: 3.431 ± 1.246
0.572ValHis: 0.572 ± 0.481
5.146ValIle: 5.146 ± 2.474
9.72ValLys: 9.72 ± 2.081
6.861ValLeu: 6.861 ± 1.746
1.715ValMet: 1.715 ± 0.728
2.859ValAsn: 2.859 ± 0.892
0.572ValPro: 0.572 ± 0.736
0.572ValGln: 0.572 ± 0.773
2.859ValArg: 2.859 ± 0.822
2.859ValSer: 2.859 ± 0.987
0.0ValThr: 0.0 ± 0.0
4.574ValVal: 4.574 ± 1.688
0.0ValTrp: 0.0 ± 0.0
2.859ValTyr: 2.859 ± 1.273
0.0ValXaa: 0.0 ± 0.0
Trp
1.715TrpAla: 1.715 ± 1.306
0.0TrpCys: 0.0 ± 0.0
1.144TrpAsp: 1.144 ± 0.779
0.572TrpGlu: 0.572 ± 0.428
2.859TrpPhe: 2.859 ± 1.731
0.0TrpGly: 0.0 ± 0.0
0.0TrpHis: 0.0 ± 0.0
3.431TrpIle: 3.431 ± 1.942
1.144TrpLys: 1.144 ± 0.881
1.144TrpLeu: 1.144 ± 0.491
1.144TrpMet: 1.144 ± 0.769
2.287TrpAsn: 2.287 ± 1.25
0.572TrpPro: 0.572 ± 0.481
1.715TrpGln: 1.715 ± 0.868
0.572TrpArg: 0.572 ± 0.481
0.0TrpSer: 0.0 ± 0.0
1.144TrpThr: 1.144 ± 1.087
1.144TrpVal: 1.144 ± 0.809
0.572TrpTrp: 0.572 ± 0.773
1.144TrpTyr: 1.144 ± 0.491
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.144TyrAla: 1.144 ± 0.556
0.0TyrCys: 0.0 ± 0.0
4.574TyrAsp: 4.574 ± 1.424
3.431TyrGlu: 3.431 ± 0.898
5.718TyrPhe: 5.718 ± 2.615
1.715TyrGly: 1.715 ± 0.948
0.572TyrHis: 0.572 ± 0.481
8.005TyrIle: 8.005 ± 2.383
3.431TyrLys: 3.431 ± 1.522
6.289TyrLeu: 6.289 ± 1.141
0.572TyrMet: 0.572 ± 0.62
4.002TyrAsn: 4.002 ± 1.654
1.144TyrPro: 1.144 ± 0.491
1.144TyrGln: 1.144 ± 0.749
3.431TyrArg: 3.431 ± 1.323
4.574TyrSer: 4.574 ± 1.523
0.572TyrThr: 0.572 ± 0.719
4.574TyrVal: 4.574 ± 1.401
1.144TyrTrp: 1.144 ± 0.576
2.859TyrTyr: 2.859 ± 1.098
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 11 proteins (1750 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski