Mogibacterium sp. CM50
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1791 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J6H8X2|J6H8X2_9FIRM Uncharacterized protein OS=Mogibacterium sp. CM50 OX=936375 GN=HMPREF1152_0636 PE=4 SV=1
MM1 pKa = 7.8 DD2 pKa = 5.42 RR3 pKa = 11.84 DD4 pKa = 3.6 KK5 pKa = 11.18 LYY7 pKa = 10.78 EE8 pKa = 4.08 ILDD11 pKa = 3.64 IEE13 pKa = 4.64 SPDD16 pKa = 3.25 EE17 pKa = 3.89 FEE19 pKa = 4.62 YY20 pKa = 11.49 YY21 pKa = 10.61 EE22 pKa = 4.23 NLSALLEE29 pKa = 4.4 ADD31 pKa = 3.65 EE32 pKa = 5.49 MIDD35 pKa = 3.88 SDD37 pKa = 6.44 LILALLKK44 pKa = 10.56 EE45 pKa = 4.28 IEE47 pKa = 4.25 LEE49 pKa = 4.03 KK50 pKa = 10.69 LADD53 pKa = 3.61 MLEE56 pKa = 4.53 SYY58 pKa = 10.73 FDD60 pKa = 3.41 EE61 pKa = 4.49 WNKK64 pKa = 10.2 VIPDD68 pKa = 4.01 EE69 pKa = 4.41 LAEE72 pKa = 4.52 LYY74 pKa = 9.27 VTIDD78 pKa = 3.42 TAKK81 pKa = 10.55 LSIMGNFNEE90 pKa = 4.13 YY91 pKa = 8.07 MTDD94 pKa = 3.31 EE95 pKa = 5.43 DD96 pKa = 4.77 YY97 pKa = 11.69 SSLAQAIYY105 pKa = 10.62 DD106 pKa = 3.51 FRR108 pKa = 11.84 RR109 pKa = 11.84 WYY111 pKa = 10.02 VLEE114 pKa = 4.19 DD115 pKa = 3.45 HH116 pKa = 6.76 VHH118 pKa = 6.6 DD119 pKa = 4.34 EE120 pKa = 4.21 EE121 pKa = 6.22 SGIEE125 pKa = 4.11 LNVRR129 pKa = 11.84 DD130 pKa = 3.97 ARR132 pKa = 11.84 YY133 pKa = 9.52 NLSASRR139 pKa = 11.84 FTGDD143 pKa = 2.77 EE144 pKa = 4.02 CNYY147 pKa = 10.36 NFDD150 pKa = 4.78 DD151 pKa = 4.77 AYY153 pKa = 11.16 QYY155 pKa = 11.57 VGDD158 pKa = 4.18 SYY160 pKa = 11.18 SVKK163 pKa = 10.33 VSDD166 pKa = 5.37 IIEE169 pKa = 4.14 AEE171 pKa = 4.31 YY172 pKa = 11.23 NN173 pKa = 3.34
Molecular weight: 20.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.744
IPC2_protein 3.783
IPC_protein 3.77
Toseland 3.567
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.656
Rodwell 3.605
Grimsley 3.478
Solomon 3.745
Lehninger 3.694
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.897
Patrickios 0.693
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.788
Protein with the highest isoelectric point:
>tr|J4WCP0|J4WCP0_9FIRM Uncharacterized protein OS=Mogibacterium sp. CM50 OX=936375 GN=HMPREF1152_0936 PE=4 SV=1
MM1 pKa = 7.38 NKK3 pKa = 10.05 SIAAIGKK10 pKa = 6.11 TGRR13 pKa = 11.84 RR14 pKa = 11.84 VMKK17 pKa = 9.79 SVRR20 pKa = 11.84 RR21 pKa = 11.84 TVRR24 pKa = 11.84 KK25 pKa = 9.62 VFPKK29 pKa = 10.05 VKK31 pKa = 10.1 KK32 pKa = 10.49 SIAKK36 pKa = 9.05 VHH38 pKa = 5.77 VKK40 pKa = 10.24 LKK42 pKa = 10.54 KK43 pKa = 9.66 KK44 pKa = 10.25 LKK46 pKa = 10.26 SVVKK50 pKa = 9.01 TFRR53 pKa = 11.84 KK54 pKa = 7.88 TNSKK58 pKa = 9.65 IRR60 pKa = 11.84 AKK62 pKa = 10.5 AKK64 pKa = 10.23 KK65 pKa = 8.7 ATSKK69 pKa = 10.59 LKK71 pKa = 9.8 RR72 pKa = 11.84 TIKK75 pKa = 10.49 HH76 pKa = 4.32 IFKK79 pKa = 10.58 RR80 pKa = 11.84 GRR82 pKa = 11.84 HH83 pKa = 4.39 RR84 pKa = 3.83
Molecular weight: 9.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.504
IPC2_protein 11.33
IPC_protein 12.837
Toseland 13.027
ProMoST 13.51
Dawson 13.027
Bjellqvist 13.013
Wikipedia 13.495
Rodwell 13.013
Grimsley 13.071
Solomon 13.51
Lehninger 13.408
Nozaki 13.027
DTASelect 13.013
Thurlkill 13.027
EMBOSS 13.525
Sillero 13.027
Patrickios 12.735
IPC_peptide 13.51
IPC2_peptide 12.501
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1791
0
1791
551351
37
2150
307.8
34.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.751 ± 0.065
1.242 ± 0.027
6.095 ± 0.054
7.14 ± 0.077
4.053 ± 0.042
7.253 ± 0.052
1.733 ± 0.025
7.928 ± 0.056
7.138 ± 0.066
8.562 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.012 ± 0.034
4.659 ± 0.046
3.215 ± 0.033
2.483 ± 0.028
4.76 ± 0.051
6.217 ± 0.048
5.189 ± 0.04
6.944 ± 0.049
0.74 ± 0.017
3.886 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here