Amino acid dipepetide frequency for Mogibacterium sp. CM50

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.561AlaAla: 7.561 ± 0.17
0.972AlaCys: 0.972 ± 0.042
4.877AlaAsp: 4.877 ± 0.093
5.739AlaGlu: 5.739 ± 0.111
3.163AlaPhe: 3.163 ± 0.103
6.078AlaGly: 6.078 ± 0.109
1.398AlaHis: 1.398 ± 0.054
5.835AlaIle: 5.835 ± 0.123
5.133AlaLys: 5.133 ± 0.111
6.803AlaLeu: 6.803 ± 0.122
2.374AlaMet: 2.374 ± 0.065
3.176AlaAsn: 3.176 ± 0.086
2.14AlaPro: 2.14 ± 0.072
1.946AlaGln: 1.946 ± 0.06
3.361AlaArg: 3.361 ± 0.095
4.202AlaSer: 4.202 ± 0.091
3.602AlaThr: 3.602 ± 0.087
5.947AlaVal: 5.947 ± 0.13
0.499AlaTrp: 0.499 ± 0.03
2.699AlaTyr: 2.699 ± 0.066
0.0AlaXaa: 0.0 ± 0.0
Cys
0.909CysAla: 0.909 ± 0.042
0.218CysCys: 0.218 ± 0.018
0.802CysAsp: 0.802 ± 0.042
0.785CysGlu: 0.785 ± 0.033
0.479CysPhe: 0.479 ± 0.028
1.313CysGly: 1.313 ± 0.053
0.252CysHis: 0.252 ± 0.022
0.978CysIle: 0.978 ± 0.044
0.785CysLys: 0.785 ± 0.041
0.833CysLeu: 0.833 ± 0.036
0.357CysMet: 0.357 ± 0.027
0.546CysAsn: 0.546 ± 0.035
0.542CysPro: 0.542 ± 0.037
0.214CysGln: 0.214 ± 0.021
0.64CysArg: 0.64 ± 0.036
0.796CysSer: 0.796 ± 0.04
0.662CysThr: 0.662 ± 0.034
0.84CysVal: 0.84 ± 0.037
0.083CysTrp: 0.083 ± 0.012
0.386CysTyr: 0.386 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
4.371AspAla: 4.371 ± 0.092
0.635AspCys: 0.635 ± 0.037
3.798AspAsp: 3.798 ± 0.093
5.39AspGlu: 5.39 ± 0.134
2.699AspPhe: 2.699 ± 0.068
4.416AspGly: 4.416 ± 0.092
0.876AspHis: 0.876 ± 0.044
5.271AspIle: 5.271 ± 0.103
4.861AspLys: 4.861 ± 0.104
4.282AspLeu: 4.282 ± 0.088
1.923AspMet: 1.923 ± 0.065
2.837AspAsn: 2.837 ± 0.077
1.758AspPro: 1.758 ± 0.061
1.048AspGln: 1.048 ± 0.04
2.929AspArg: 2.929 ± 0.071
3.557AspSer: 3.557 ± 0.09
2.844AspThr: 2.844 ± 0.074
4.364AspVal: 4.364 ± 0.104
0.501AspTrp: 0.501 ± 0.029
2.595AspTyr: 2.595 ± 0.072
0.0AspXaa: 0.0 ± 0.0
Glu
5.657GluAla: 5.657 ± 0.117
0.762GluCys: 0.762 ± 0.043
3.823GluAsp: 3.823 ± 0.097
5.358GluGlu: 5.358 ± 0.14
2.786GluPhe: 2.786 ± 0.085
3.99GluGly: 3.99 ± 0.083
1.485GluHis: 1.485 ± 0.048
5.933GluIle: 5.933 ± 0.123
5.374GluLys: 5.374 ± 0.121
7.304GluLeu: 7.304 ± 0.154
2.18GluMet: 2.18 ± 0.059
3.725GluAsn: 3.725 ± 0.096
1.921GluPro: 1.921 ± 0.067
2.305GluGln: 2.305 ± 0.073
3.568GluArg: 3.568 ± 0.096
3.667GluSer: 3.667 ± 0.099
3.261GluThr: 3.261 ± 0.079
4.616GluVal: 4.616 ± 0.118
0.511GluTrp: 0.511 ± 0.028
3.174GluTyr: 3.174 ± 0.077
0.0GluXaa: 0.0 ± 0.0
Phe
3.194PheAla: 3.194 ± 0.08
0.579PheCys: 0.579 ± 0.032
2.751PheAsp: 2.751 ± 0.079
2.646PheGlu: 2.646 ± 0.072
1.777PhePhe: 1.777 ± 0.071
3.069PheGly: 3.069 ± 0.08
0.611PheHis: 0.611 ± 0.036
3.096PheIle: 3.096 ± 0.082
2.73PheLys: 2.73 ± 0.078
3.179PheLeu: 3.179 ± 0.097
1.193PheMet: 1.193 ± 0.053
1.93PheAsn: 1.93 ± 0.063
1.311PhePro: 1.311 ± 0.044
0.825PheGln: 0.825 ± 0.041
1.81PheArg: 1.81 ± 0.068
2.76PheSer: 2.76 ± 0.066
2.245PheThr: 2.245 ± 0.076
2.958PheVal: 2.958 ± 0.069
0.348PheTrp: 0.348 ± 0.025
1.514PheTyr: 1.514 ± 0.057
0.0PheXaa: 0.0 ± 0.0
Gly
5.434GlyAla: 5.434 ± 0.108
1.085GlyCys: 1.085 ± 0.05
3.925GlyAsp: 3.925 ± 0.086
4.772GlyGlu: 4.772 ± 0.103
3.13GlyPhe: 3.13 ± 0.085
5.176GlyGly: 5.176 ± 0.122
1.304GlyHis: 1.304 ± 0.05
6.413GlyIle: 6.413 ± 0.134
5.686GlyLys: 5.686 ± 0.123
5.768GlyLeu: 5.768 ± 0.125
2.215GlyMet: 2.215 ± 0.063
3.403GlyAsn: 3.403 ± 0.075
1.239GlyPro: 1.239 ± 0.047
1.837GlyGln: 1.837 ± 0.063
3.482GlyArg: 3.482 ± 0.094
4.362GlySer: 4.362 ± 0.084
4.246GlyThr: 4.246 ± 0.102
5.213GlyVal: 5.213 ± 0.112
0.537GlyTrp: 0.537 ± 0.039
3.303GlyTyr: 3.303 ± 0.069
0.0GlyXaa: 0.0 ± 0.0
His
1.063HisAla: 1.063 ± 0.044
0.229HisCys: 0.229 ± 0.021
1.066HisAsp: 1.066 ± 0.054
1.173HisGlu: 1.173 ± 0.05
0.684HisPhe: 0.684 ± 0.036
1.404HisGly: 1.404 ± 0.049
0.401HisHis: 0.401 ± 0.049
1.406HisIle: 1.406 ± 0.053
1.137HisLys: 1.137 ± 0.047
1.409HisLeu: 1.409 ± 0.047
0.501HisMet: 0.501 ± 0.033
0.802HisAsn: 0.802 ± 0.037
0.825HisPro: 0.825 ± 0.048
0.423HisGln: 0.423 ± 0.028
0.867HisArg: 0.867 ± 0.04
1.03HisSer: 1.03 ± 0.043
0.901HisThr: 0.901 ± 0.044
1.148HisVal: 1.148 ± 0.052
0.116HisTrp: 0.116 ± 0.017
0.744HisTyr: 0.744 ± 0.042
0.0HisXaa: 0.0 ± 0.0
Ile
6.685IleAla: 6.685 ± 0.109
1.175IleCys: 1.175 ± 0.046
5.117IleAsp: 5.117 ± 0.103
5.41IleGlu: 5.41 ± 0.116
3.038IlePhe: 3.038 ± 0.088
5.632IleGly: 5.632 ± 0.117
1.21IleHis: 1.21 ± 0.049
6.038IleIle: 6.038 ± 0.122
5.423IleLys: 5.423 ± 0.109
6.444IleLeu: 6.444 ± 0.129
2.106IleMet: 2.106 ± 0.068
3.577IleAsn: 3.577 ± 0.087
3.21IlePro: 3.21 ± 0.079
1.609IleGln: 1.609 ± 0.057
3.856IleArg: 3.856 ± 0.094
5.488IleSer: 5.488 ± 0.114
4.398IleThr: 4.398 ± 0.084
5.597IleVal: 5.597 ± 0.111
0.477IleTrp: 0.477 ± 0.03
2.786IleTyr: 2.786 ± 0.074
0.0IleXaa: 0.0 ± 0.0
Lys
5.51LysAla: 5.51 ± 0.107
0.709LysCys: 0.709 ± 0.039
4.353LysAsp: 4.353 ± 0.108
5.512LysGlu: 5.512 ± 0.134
2.51LysPhe: 2.51 ± 0.069
4.513LysGly: 4.513 ± 0.11
1.261LysHis: 1.261 ± 0.047
4.872LysIle: 4.872 ± 0.099
5.612LysLys: 5.612 ± 0.128
6.484LysLeu: 6.484 ± 0.108
2.131LysMet: 2.131 ± 0.056
3.609LysAsn: 3.609 ± 0.099
2.097LysPro: 2.097 ± 0.067
2.149LysGln: 2.149 ± 0.062
3.569LysArg: 3.569 ± 0.092
4.366LysSer: 4.366 ± 0.107
3.869LysThr: 3.869 ± 0.101
4.674LysVal: 4.674 ± 0.098
0.557LysTrp: 0.557 ± 0.034
3.027LysTyr: 3.027 ± 0.079
0.0LysXaa: 0.0 ± 0.0
Leu
6.675LeuAla: 6.675 ± 0.108
1.068LeuCys: 1.068 ± 0.044
5.078LeuAsp: 5.078 ± 0.11
5.773LeuGlu: 5.773 ± 0.118
3.337LeuPhe: 3.337 ± 0.101
6.118LeuGly: 6.118 ± 0.117
1.498LeuHis: 1.498 ± 0.053
6.484LeuIle: 6.484 ± 0.151
6.032LeuLys: 6.032 ± 0.125
7.453LeuLeu: 7.453 ± 0.148
2.806LeuMet: 2.806 ± 0.087
3.812LeuAsn: 3.812 ± 0.089
3.315LeuPro: 3.315 ± 0.085
2.182LeuGln: 2.182 ± 0.06
4.088LeuArg: 4.088 ± 0.101
6.314LeuSer: 6.314 ± 0.117
4.369LeuThr: 4.369 ± 0.079
5.586LeuVal: 5.586 ± 0.115
0.577LeuTrp: 0.577 ± 0.033
3.053LeuTyr: 3.053 ± 0.071
0.0LeuXaa: 0.0 ± 0.0
Met
2.352MetAla: 2.352 ± 0.064
0.336MetCys: 0.336 ± 0.026
1.725MetAsp: 1.725 ± 0.056
1.801MetGlu: 1.801 ± 0.063
1.099MetPhe: 1.099 ± 0.044
2.048MetGly: 2.048 ± 0.063
0.484MetHis: 0.484 ± 0.026
2.378MetIle: 2.378 ± 0.078
2.283MetLys: 2.283 ± 0.065
2.704MetLeu: 2.704 ± 0.078
1.059MetMet: 1.059 ± 0.047
1.65MetAsn: 1.65 ± 0.052
1.139MetPro: 1.139 ± 0.042
0.954MetGln: 0.954 ± 0.042
1.471MetArg: 1.471 ± 0.054
2.077MetSer: 2.077 ± 0.053
1.707MetThr: 1.707 ± 0.056
1.732MetVal: 1.732 ± 0.059
0.183MetTrp: 0.183 ± 0.017
0.932MetTyr: 0.932 ± 0.041
0.0MetXaa: 0.0 ± 0.0
Asn
3.315AsnAla: 3.315 ± 0.078
0.508AsnCys: 0.508 ± 0.031
2.536AsnAsp: 2.536 ± 0.073
3.107AsnGlu: 3.107 ± 0.071
1.803AsnPhe: 1.803 ± 0.058
3.566AsnGly: 3.566 ± 0.091
0.811AsnHis: 0.811 ± 0.033
4.048AsnIle: 4.048 ± 0.09
3.39AsnLys: 3.39 ± 0.08
3.843AsnLeu: 3.843 ± 0.1
1.337AsnMet: 1.337 ± 0.047
2.293AsnAsn: 2.293 ± 0.078
2.115AsnPro: 2.115 ± 0.08
1.037AsnGln: 1.037 ± 0.048
2.383AsnArg: 2.383 ± 0.072
2.853AsnSer: 2.853 ± 0.069
2.403AsnThr: 2.403 ± 0.062
3.062AsnVal: 3.062 ± 0.075
0.428AsnTrp: 0.428 ± 0.029
1.756AsnTyr: 1.756 ± 0.054
0.0AsnXaa: 0.0 ± 0.0
Pro
2.543ProAla: 2.543 ± 0.067
0.386ProCys: 0.386 ± 0.027
2.445ProAsp: 2.445 ± 0.074
2.945ProGlu: 2.945 ± 0.076
1.397ProPhe: 1.397 ± 0.048
2.39ProGly: 2.39 ± 0.069
0.609ProHis: 0.609 ± 0.035
2.398ProIle: 2.398 ± 0.062
2.004ProLys: 2.004 ± 0.071
2.528ProLeu: 2.528 ± 0.066
0.887ProMet: 0.887 ± 0.04
1.411ProAsn: 1.411 ± 0.048
0.7ProPro: 0.7 ± 0.036
0.851ProGln: 0.851 ± 0.043
1.128ProArg: 1.128 ± 0.051
1.865ProSer: 1.865 ± 0.058
1.65ProThr: 1.65 ± 0.055
2.519ProVal: 2.519 ± 0.067
0.248ProTrp: 0.248 ± 0.018
1.246ProTyr: 1.246 ± 0.053
0.0ProXaa: 0.0 ± 0.0
Gln
1.903GlnAla: 1.903 ± 0.07
0.272GlnCys: 0.272 ± 0.026
1.3GlnAsp: 1.3 ± 0.049
1.64GlnGlu: 1.64 ± 0.062
0.941GlnPhe: 0.941 ± 0.043
1.654GlnGly: 1.654 ± 0.05
0.415GlnHis: 0.415 ± 0.025
2.113GlnIle: 2.113 ± 0.066
1.787GlnLys: 1.787 ± 0.065
2.43GlnLeu: 2.43 ± 0.065
0.803GlnMet: 0.803 ± 0.036
1.213GlnAsn: 1.213 ± 0.052
0.727GlnPro: 0.727 ± 0.038
0.813GlnGln: 0.813 ± 0.044
1.259GlnArg: 1.259 ± 0.049
1.582GlnSer: 1.582 ± 0.059
1.233GlnThr: 1.233 ± 0.058
1.567GlnVal: 1.567 ± 0.053
0.187GlnTrp: 0.187 ± 0.018
0.987GlnTyr: 0.987 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
3.292ArgAla: 3.292 ± 0.084
0.513ArgCys: 0.513 ± 0.032
2.779ArgAsp: 2.779 ± 0.071
4.003ArgGlu: 4.003 ± 0.101
1.921ArgPhe: 1.921 ± 0.062
3.04ArgGly: 3.04 ± 0.085
0.798ArgHis: 0.798 ± 0.034
3.907ArgIle: 3.907 ± 0.104
3.606ArgLys: 3.606 ± 0.09
4.159ArgLeu: 4.159 ± 0.099
1.585ArgMet: 1.585 ± 0.055
2.354ArgAsn: 2.354 ± 0.072
1.348ArgPro: 1.348 ± 0.053
1.346ArgGln: 1.346 ± 0.042
2.59ArgArg: 2.59 ± 0.079
2.686ArgSer: 2.686 ± 0.081
2.371ArgThr: 2.371 ± 0.07
2.962ArgVal: 2.962 ± 0.072
0.366ArgTrp: 0.366 ± 0.029
1.977ArgTyr: 1.977 ± 0.06
0.0ArgXaa: 0.0 ± 0.0
Ser
4.373SerAla: 4.373 ± 0.092
0.725SerCys: 0.725 ± 0.035
3.983SerAsp: 3.983 ± 0.1
4.329SerGlu: 4.329 ± 0.095
2.775SerPhe: 2.775 ± 0.077
5.376SerGly: 5.376 ± 0.113
1.117SerHis: 1.117 ± 0.043
4.89SerIle: 4.89 ± 0.115
4.358SerLys: 4.358 ± 0.109
5.24SerLeu: 5.24 ± 0.106
1.783SerMet: 1.783 ± 0.058
2.764SerAsn: 2.764 ± 0.083
1.834SerPro: 1.834 ± 0.058
1.433SerGln: 1.433 ± 0.055
2.924SerArg: 2.924 ± 0.064
3.921SerSer: 3.921 ± 0.096
3.082SerThr: 3.082 ± 0.08
4.302SerVal: 4.302 ± 0.089
0.531SerTrp: 0.531 ± 0.026
2.429SerTyr: 2.429 ± 0.064
0.0SerXaa: 0.0 ± 0.0
Thr
4.318ThrAla: 4.318 ± 0.089
0.642ThrCys: 0.642 ± 0.04
3.063ThrAsp: 3.063 ± 0.085
3.457ThrGlu: 3.457 ± 0.091
2.08ThrPhe: 2.08 ± 0.064
4.62ThrGly: 4.62 ± 0.108
0.903ThrHis: 0.903 ± 0.041
3.95ThrIle: 3.95 ± 0.091
3.114ThrLys: 3.114 ± 0.08
4.603ThrLeu: 4.603 ± 0.098
1.424ThrMet: 1.424 ± 0.053
1.973ThrAsn: 1.973 ± 0.065
2.089ThrPro: 2.089 ± 0.067
1.148ThrGln: 1.148 ± 0.042
2.069ThrArg: 2.069 ± 0.063
3.054ThrSer: 3.054 ± 0.077
2.706ThrThr: 2.706 ± 0.082
4.406ThrVal: 4.406 ± 0.091
0.412ThrTrp: 0.412 ± 0.029
1.861ThrTyr: 1.861 ± 0.066
0.0ThrXaa: 0.0 ± 0.0
Val
5.242ValAla: 5.242 ± 0.11
1.012ValCys: 1.012 ± 0.049
4.304ValAsp: 4.304 ± 0.098
4.455ValGlu: 4.455 ± 0.111
2.895ValPhe: 2.895 ± 0.087
4.638ValGly: 4.638 ± 0.109
1.079ValHis: 1.079 ± 0.049
5.828ValIle: 5.828 ± 0.127
4.919ValLys: 4.919 ± 0.119
6.23ValLeu: 6.23 ± 0.115
2.055ValMet: 2.055 ± 0.059
3.285ValAsn: 3.285 ± 0.083
2.421ValPro: 2.421 ± 0.068
1.534ValGln: 1.534 ± 0.05
3.163ValArg: 3.163 ± 0.082
4.641ValSer: 4.641 ± 0.1
3.851ValThr: 3.851 ± 0.09
4.948ValVal: 4.948 ± 0.115
0.466ValTrp: 0.466 ± 0.03
2.478ValTyr: 2.478 ± 0.058
0.0ValXaa: 0.0 ± 0.0
Trp
0.446TrpAla: 0.446 ± 0.026
0.096TrpCys: 0.096 ± 0.014
0.473TrpAsp: 0.473 ± 0.028
0.448TrpGlu: 0.448 ± 0.029
0.334TrpPhe: 0.334 ± 0.027
0.521TrpGly: 0.521 ± 0.032
0.145TrpHis: 0.145 ± 0.016
0.582TrpIle: 0.582 ± 0.03
0.55TrpLys: 0.55 ± 0.032
0.673TrpLeu: 0.673 ± 0.038
0.258TrpMet: 0.258 ± 0.024
0.406TrpAsn: 0.406 ± 0.026
0.187TrpPro: 0.187 ± 0.02
0.287TrpGln: 0.287 ± 0.024
0.336TrpArg: 0.336 ± 0.026
0.385TrpSer: 0.385 ± 0.023
0.359TrpThr: 0.359 ± 0.027
0.463TrpVal: 0.463 ± 0.028
0.098TrpTrp: 0.098 ± 0.013
0.355TrpTyr: 0.355 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.663TyrAla: 2.663 ± 0.068
0.499TyrCys: 0.499 ± 0.032
2.755TyrAsp: 2.755 ± 0.071
2.657TyrGlu: 2.657 ± 0.069
1.681TyrPhe: 1.681 ± 0.057
2.969TyrGly: 2.969 ± 0.072
0.669TyrHis: 0.669 ± 0.04
2.858TyrIle: 2.858 ± 0.072
2.599TyrLys: 2.599 ± 0.071
3.248TyrLeu: 3.248 ± 0.073
1.137TyrMet: 1.137 ± 0.047
1.826TyrAsn: 1.826 ± 0.058
1.233TyrPro: 1.233 ± 0.048
0.885TyrGln: 0.885 ± 0.037
2.108TyrArg: 2.108 ± 0.071
2.563TyrSer: 2.563 ± 0.064
2.135TyrThr: 2.135 ± 0.068
2.541TyrVal: 2.541 ± 0.072
0.274TyrTrp: 0.274 ± 0.024
1.558TyrTyr: 1.558 ± 0.052
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1791 proteins (551351 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski