Ajellomyces capsulatus (strain H88) (Darling s disease fungus) (Histoplasma capsulatum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales; Ajellomycetaceae; Histoplasma; Histoplasma capsulatum

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9445 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0UHR3|F0UHR3_AJEC8 Patatin-like phospholipase domain-containing protein OS=Ajellomyces capsulatus (strain H88) OX=544711 GN=HCEG_05487 PE=3 SV=1
MM1 pKa = 7.67FSLTDD6 pKa = 4.04LSAADD11 pKa = 4.05LDD13 pKa = 4.39YY14 pKa = 11.0FSSHH18 pKa = 7.54IILTVHH24 pKa = 6.36NADD27 pKa = 4.23AFAINNNDD35 pKa = 3.35ADD37 pKa = 3.54QSYY40 pKa = 11.06EE41 pKa = 4.0KK42 pKa = 10.66FII44 pKa = 5.05

Molecular weight: 4.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0UV39|F0UV39_AJEC8 Uncharacterized protein OS=Ajellomyces capsulatus (strain H88) OX=544711 GN=HCEG_08981 PE=4 SV=1
MM1 pKa = 7.5NVRR4 pKa = 11.84CLQVGIHH11 pKa = 6.15GFININISARR21 pKa = 11.84ANRR24 pKa = 11.84RR25 pKa = 11.84SSPSKK30 pKa = 9.81LSVYY34 pKa = 7.01VQCVQAYY41 pKa = 7.43PPPTLLSSVAYY52 pKa = 8.82PRR54 pKa = 11.84TIIPTTSAKK63 pKa = 10.42GRR65 pKa = 11.84DD66 pKa = 3.59IVLKK70 pKa = 10.31RR71 pKa = 11.84APEE74 pKa = 4.29RR75 pKa = 11.84DD76 pKa = 3.72DD77 pKa = 5.63KK78 pKa = 11.35ILEE81 pKa = 4.24TDD83 pKa = 3.04HH84 pKa = 7.41DD85 pKa = 4.18IPRR88 pKa = 11.84PSQGLLPSPSGAWHH102 pKa = 7.25PSQICQSSWAHH113 pKa = 6.7SNFQGCSIHH122 pKa = 6.38SQAARR127 pKa = 11.84DD128 pKa = 3.72VARR131 pKa = 11.84LKK133 pKa = 10.35IHH135 pKa = 6.01VGKK138 pKa = 8.49TRR140 pKa = 11.84WNGQEE145 pKa = 3.23IDD147 pKa = 3.36PRR149 pKa = 11.84RR150 pKa = 11.84RR151 pKa = 11.84GAQHH155 pKa = 6.41SFSLVHH161 pKa = 6.14NRR163 pKa = 11.84HH164 pKa = 5.62GGCWDD169 pKa = 3.32RR170 pKa = 11.84TTCRR174 pKa = 11.84MPQDD178 pKa = 3.53AKK180 pKa = 10.8SISSARR186 pKa = 11.84SHH188 pKa = 7.48PIPATEE194 pKa = 3.98RR195 pKa = 11.84ADD197 pKa = 3.53VPVGIVVYY205 pKa = 7.21EE206 pKa = 3.97TRR208 pKa = 11.84PQHH211 pKa = 6.23WDD213 pKa = 3.01PAAEE217 pKa = 4.01RR218 pKa = 11.84GSRR221 pKa = 11.84GWEE224 pKa = 3.78TKK226 pKa = 10.46VIEE229 pKa = 4.66GSQPQRR235 pKa = 11.84KK236 pKa = 8.16FFNFSVPTTHH246 pKa = 7.5RR247 pKa = 11.84VGIPLHH253 pKa = 6.27ARR255 pKa = 11.84AILAIIHH262 pKa = 5.71RR263 pKa = 11.84TFFLQNNPLLLASPP277 pKa = 4.76

Molecular weight: 30.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9445

0

9445

4200093

39

5717

444.7

49.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.907 ± 0.018

1.277 ± 0.009

5.54 ± 0.017

6.118 ± 0.026

3.642 ± 0.014

6.725 ± 0.023

2.522 ± 0.011

5.102 ± 0.016

4.996 ± 0.02

8.875 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.091 ± 0.009

3.967 ± 0.013

6.289 ± 0.029

4.075 ± 0.018

6.452 ± 0.021

8.831 ± 0.03

5.857 ± 0.016

5.787 ± 0.018

1.326 ± 0.009

2.62 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski