Gossypium arboreum (Tree cotton) (Gossypium nanking)
Average proteome isoelectric point is 7.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 31431 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B0NVE4|A0A0B0NVE4_GOSAR Uncharacterized protein OS=Gossypium arboreum OX=29729 GN=F383_25827 PE=4 SV=1
MM1 pKa = 7.34 NSDD4 pKa = 3.8 HH5 pKa = 7.21 SMSSDD10 pKa = 2.58 ATYY13 pKa = 8.95 ITNPDD18 pKa = 3.2 HH19 pKa = 7.61 SMSSDD24 pKa = 3.41 FLIPSDD30 pKa = 3.61 MSLVSS35 pKa = 3.39
Molecular weight: 3.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.91
IPC_protein 3.732
Toseland 3.528
ProMoST 3.999
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.859
Rodwell 3.592
Grimsley 3.465
Solomon 3.732
Lehninger 3.694
Nozaki 3.961
DTASelect 4.24
Thurlkill 3.668
EMBOSS 3.846
Sillero 3.884
Patrickios 1.952
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.788
Protein with the highest isoelectric point:
>tr|A0A0B0PCS6|A0A0B0PCS6_GOSAR Isoform of A0A0B0PHC0 Calcium-transporting ATPase OS=Gossypium arboreum OX=29729 GN=F383_00749 PE=3 SV=1
MM1 pKa = 7.76 RR2 pKa = 11.84 WMFWWFRR9 pKa = 11.84 VIWGFHH15 pKa = 4.63 HH16 pKa = 7.42 RR17 pKa = 11.84 RR18 pKa = 11.84 LRR20 pKa = 11.84 WFRR23 pKa = 11.84 VIRR26 pKa = 11.84 WLHH29 pKa = 5.19 HH30 pKa = 5.13 RR31 pKa = 11.84 WLRR34 pKa = 11.84 VIGWLYY40 pKa = 9.47 NGRR43 pKa = 11.84 MMMFRR48 pKa = 11.84 RR49 pKa = 11.84 IAWRR53 pKa = 11.84 RR54 pKa = 11.84 TFRR57 pKa = 11.84 RR58 pKa = 11.84 RR59 pKa = 11.84 RR60 pKa = 11.84 WVAVTFRR67 pKa = 11.84 AAA69 pKa = 3.2
Molecular weight: 9.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.509
IPC2_protein 11.316
IPC_protein 12.778
Toseland 12.925
ProMoST 13.437
Dawson 12.925
Bjellqvist 12.925
Wikipedia 13.408
Rodwell 12.427
Grimsley 12.969
Solomon 13.437
Lehninger 13.334
Nozaki 12.925
DTASelect 12.925
Thurlkill 12.925
EMBOSS 13.437
Sillero 12.925
Patrickios 12.149
IPC_peptide 13.437
IPC2_peptide 12.427
IPC2.peptide.svr19 9.204
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
30420
1011
31431
9014226
8
5456
286.8
32.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.747 ± 0.014
1.911 ± 0.007
5.198 ± 0.012
6.407 ± 0.018
4.073 ± 0.01
6.495 ± 0.015
2.447 ± 0.007
5.328 ± 0.011
6.19 ± 0.018
9.587 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.594 ± 0.006
4.411 ± 0.011
4.944 ± 0.014
3.771 ± 0.011
5.346 ± 0.012
9.022 ± 0.021
4.94 ± 0.01
6.472 ± 0.011
1.285 ± 0.006
2.833 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here