Salinarimonas sp. BN140002
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5046 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B2VVA8|A0A5B2VVA8_9RHIZ Uncharacterized protein OS=Salinarimonas sp. BN140002 OX=1638099 GN=F0L46_02905 PE=4 SV=1
MM1 pKa = 7.47 QIGANQQNIDD11 pKa = 3.52 TSQWASLDD19 pKa = 3.5 GKK21 pKa = 10.82 AGLSFNDD28 pKa = 3.47 ILMGMLQLLQSGQRR42 pKa = 11.84 VGEE45 pKa = 4.2 GAGGGAAGEE54 pKa = 4.22 AGGCGAAGSATGCGGAGGAGGIGDD78 pKa = 4.53 AGATNACGGANGSDD92 pKa = 3.44 NGILMPSQAGGAGAVGSSGSCGGMDD117 pKa = 3.4 GGYY120 pKa = 10.78 DD121 pKa = 3.69 FGSDD125 pKa = 3.41 SLDD128 pKa = 3.36 AAGGCGSSSGVGEE141 pKa = 4.03 AGGCGEE147 pKa = 4.31 VGATGSDD154 pKa = 3.11 VDD156 pKa = 4.08 GASGAGEE163 pKa = 4.37 GCPRR167 pKa = 11.84 CCGPQCSCNGNASRR181 pKa = 11.84 NGVSVFRR188 pKa = 11.84 GNKK191 pKa = 8.73 LQQ193 pKa = 3.54
Molecular weight: 17.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.865
IPC2_protein 3.859
IPC_protein 3.808
Toseland 3.592
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.783
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.757
Nozaki 3.935
DTASelect 4.19
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.935
Patrickios 0.146
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|A0A5B2UZ78|A0A5B2UZ78_9RHIZ Uncharacterized protein OS=Salinarimonas sp. BN140002 OX=1638099 GN=F0L46_25280 PE=4 SV=1
GG1 pKa = 6.88 GRR3 pKa = 11.84 GAGGGGGSPRR13 pKa = 11.84 VPPLAPRR20 pKa = 11.84 PPAPPPPPRR29 pKa = 11.84 RR30 pKa = 11.84 GGRR33 pKa = 11.84 PPPPVAPPPRR43 pKa = 11.84 GPRR46 pKa = 11.84 SRR48 pKa = 11.84 GGGGLGRR55 pKa = 11.84 PPLAPARR62 pKa = 11.84 RR63 pKa = 11.84 GAPPPP68 pKa = 3.77
Molecular weight: 6.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.544
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.281
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.236
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5046
0
5046
1511247
30
2108
299.5
32.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.867 ± 0.054
0.778 ± 0.011
5.467 ± 0.028
5.787 ± 0.031
3.393 ± 0.022
9.191 ± 0.035
1.929 ± 0.017
4.404 ± 0.024
2.383 ± 0.027
10.443 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.219 ± 0.014
2.066 ± 0.02
5.858 ± 0.028
2.681 ± 0.021
8.493 ± 0.038
4.728 ± 0.02
5.115 ± 0.023
7.953 ± 0.032
1.275 ± 0.014
1.969 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here