Mycobacterium phage Quesadilla

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; unclassified Bclasvirinae

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WF65|A0A5Q2WF65_9CAUD Uncharacterized protein OS=Mycobacterium phage Quesadilla OX=2664226 GN=81 PE=4 SV=1
MM1 pKa = 7.37FSHH4 pKa = 6.64YY5 pKa = 9.13SARR8 pKa = 11.84YY9 pKa = 7.63AAEE12 pKa = 4.41GGPGVTLPTIPEE24 pKa = 3.72AMIADD29 pKa = 3.84EE30 pKa = 4.41TANVMSIVFAGAGRR44 pKa = 11.84WVLADD49 pKa = 3.39SQGQVYY55 pKa = 10.57AEE57 pKa = 4.24AEE59 pKa = 4.19NPAEE63 pKa = 4.3LGDD66 pKa = 3.84WLTEE70 pKa = 3.98YY71 pKa = 11.1LDD73 pKa = 3.75TLGQMVEE80 pKa = 4.01RR81 pKa = 11.84GEE83 pKa = 4.09LL84 pKa = 3.41

Molecular weight: 9.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WEM7|A0A5Q2WEM7_9CAUD Uncharacterized protein OS=Mycobacterium phage Quesadilla OX=2664226 GN=86 PE=4 SV=1
MM1 pKa = 7.57ARR3 pKa = 11.84RR4 pKa = 11.84GPASSRR10 pKa = 11.84RR11 pKa = 11.84GGRR14 pKa = 11.84RR15 pKa = 11.84ALSKK19 pKa = 10.58SSPKK23 pKa = 9.17IARR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84GTAVAMSAATGGIGQFSRR46 pKa = 11.84RR47 pKa = 11.84GGGSYY52 pKa = 10.25HH53 pKa = 6.19GFKK56 pKa = 10.55SKK58 pKa = 10.65KK59 pKa = 5.56QWRR62 pKa = 11.84WAFASKK68 pKa = 10.47QPWAHH73 pKa = 6.12EE74 pKa = 4.21KK75 pKa = 9.98AHH77 pKa = 5.73KK78 pKa = 9.32TKK80 pKa = 10.56GGPKK84 pKa = 9.34ARR86 pKa = 11.84YY87 pKa = 8.96RR88 pKa = 11.84RR89 pKa = 11.84LPASKK94 pKa = 10.38HH95 pKa = 5.76SGHH98 pKa = 6.59KK99 pKa = 10.25GSRR102 pKa = 11.84APSS105 pKa = 3.23

Molecular weight: 11.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

22765

53

2127

232.3

24.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.046 ± 0.355

0.922 ± 0.127

5.746 ± 0.237

6.51 ± 0.336

2.583 ± 0.11

9.655 ± 0.503

1.968 ± 0.179

4.639 ± 0.165

2.93 ± 0.19

8.39 ± 0.199

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.416 ± 0.119

2.658 ± 0.15

6.418 ± 0.226

3.532 ± 0.201

6.791 ± 0.373

5.359 ± 0.163

5.741 ± 0.189

6.703 ± 0.178

1.959 ± 0.161

2.034 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski