Amino acid dipepetide frequency for Mycobacterium phage Quesadilla

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.055AlaAla: 18.055 ± 1.356
0.527AlaCys: 0.527 ± 0.146
7.468AlaAsp: 7.468 ± 0.762
10.148AlaGlu: 10.148 ± 0.828
2.68AlaPhe: 2.68 ± 0.378
11.773AlaGly: 11.773 ± 1.144
2.153AlaHis: 2.153 ± 0.298
5.447AlaIle: 5.447 ± 0.454
4.613AlaLys: 4.613 ± 0.641
10.763AlaLeu: 10.763 ± 0.851
3.602AlaMet: 3.602 ± 0.412
3.207AlaAsn: 3.207 ± 0.408
8.347AlaPro: 8.347 ± 0.664
4.788AlaGln: 4.788 ± 0.458
8.874AlaArg: 8.874 ± 0.848
7.073AlaSer: 7.073 ± 0.746
7.204AlaThr: 7.204 ± 0.582
8.83AlaVal: 8.83 ± 0.666
2.328AlaTrp: 2.328 ± 0.31
2.592AlaTyr: 2.592 ± 0.301
0.0AlaXaa: 0.0 ± 0.0
Cys
1.054CysAla: 1.054 ± 0.229
0.0CysCys: 0.0 ± 0.0
0.835CysAsp: 0.835 ± 0.203
0.22CysGlu: 0.22 ± 0.107
0.22CysPhe: 0.22 ± 0.115
1.362CysGly: 1.362 ± 0.287
0.308CysHis: 0.308 ± 0.113
0.132CysIle: 0.132 ± 0.071
0.176CysLys: 0.176 ± 0.086
0.308CysLeu: 0.308 ± 0.117
0.22CysMet: 0.22 ± 0.107
0.132CysAsn: 0.132 ± 0.077
0.879CysPro: 0.879 ± 0.217
0.22CysGln: 0.22 ± 0.112
1.186CysArg: 1.186 ± 0.217
0.659CysSer: 0.659 ± 0.172
0.176CysThr: 0.176 ± 0.082
0.703CysVal: 0.703 ± 0.188
0.22CysTrp: 0.22 ± 0.102
0.22CysTyr: 0.22 ± 0.113
0.0CysXaa: 0.0 ± 0.0
Asp
6.677AspAla: 6.677 ± 0.58
0.703AspCys: 0.703 ± 0.224
3.251AspAsp: 3.251 ± 0.438
3.954AspGlu: 3.954 ± 0.584
1.757AspPhe: 1.757 ± 0.231
5.667AspGly: 5.667 ± 0.609
1.845AspHis: 1.845 ± 0.339
3.075AspIle: 3.075 ± 0.316
1.494AspLys: 1.494 ± 0.262
4.393AspLeu: 4.393 ± 0.427
1.186AspMet: 1.186 ± 0.227
1.23AspAsn: 1.23 ± 0.312
4.876AspPro: 4.876 ± 0.551
2.372AspGln: 2.372 ± 0.383
3.69AspArg: 3.69 ± 0.451
2.504AspSer: 2.504 ± 0.415
2.855AspThr: 2.855 ± 0.328
3.69AspVal: 3.69 ± 0.324
1.01AspTrp: 1.01 ± 0.196
1.23AspTyr: 1.23 ± 0.256
0.0AspXaa: 0.0 ± 0.0
Glu
9.225GluAla: 9.225 ± 0.762
0.966GluCys: 0.966 ± 0.24
3.075GluAsp: 3.075 ± 0.449
3.69GluGlu: 3.69 ± 0.519
1.713GluPhe: 1.713 ± 0.308
5.755GluGly: 5.755 ± 0.64
1.538GluHis: 1.538 ± 0.293
3.031GluIle: 3.031 ± 0.362
1.977GluLys: 1.977 ± 0.299
6.897GluLeu: 6.897 ± 0.611
1.362GluMet: 1.362 ± 0.302
1.318GluAsn: 1.318 ± 0.223
3.778GluPro: 3.778 ± 0.597
2.196GluGln: 2.196 ± 0.35
5.711GluArg: 5.711 ± 0.687
2.328GluSer: 2.328 ± 0.27
3.383GluThr: 3.383 ± 0.322
5.184GluVal: 5.184 ± 0.6
1.01GluTrp: 1.01 ± 0.156
0.966GluTyr: 0.966 ± 0.179
0.0GluXaa: 0.0 ± 0.0
Phe
3.075PheAla: 3.075 ± 0.33
0.22PheCys: 0.22 ± 0.099
2.065PheAsp: 2.065 ± 0.417
1.889PheGlu: 1.889 ± 0.331
0.659PhePhe: 0.659 ± 0.166
2.636PheGly: 2.636 ± 0.356
0.879PheHis: 0.879 ± 0.18
1.054PheIle: 1.054 ± 0.206
0.571PheLys: 0.571 ± 0.156
1.713PheLeu: 1.713 ± 0.268
0.483PheMet: 0.483 ± 0.128
0.747PheAsn: 0.747 ± 0.199
1.362PhePro: 1.362 ± 0.25
0.923PheGln: 0.923 ± 0.215
1.801PheArg: 1.801 ± 0.273
1.977PheSer: 1.977 ± 0.322
1.45PheThr: 1.45 ± 0.276
1.538PheVal: 1.538 ± 0.239
0.439PheTrp: 0.439 ± 0.149
0.351PheTyr: 0.351 ± 0.142
0.0PheXaa: 0.0 ± 0.0
Gly
10.06GlyAla: 10.06 ± 1.341
1.186GlyCys: 1.186 ± 0.294
4.832GlyAsp: 4.832 ± 0.408
5.974GlyGlu: 5.974 ± 0.627
2.636GlyPhe: 2.636 ± 0.468
10.85GlyGly: 10.85 ± 2.145
1.757GlyHis: 1.757 ± 0.347
4.613GlyIle: 4.613 ± 0.502
3.426GlyLys: 3.426 ± 0.493
8.654GlyLeu: 8.654 ± 0.881
2.153GlyMet: 2.153 ± 0.592
2.592GlyAsn: 2.592 ± 0.405
4.788GlyPro: 4.788 ± 0.568
3.822GlyGln: 3.822 ± 0.496
6.37GlyArg: 6.37 ± 0.55
5.579GlySer: 5.579 ± 0.528
5.535GlyThr: 5.535 ± 0.599
6.677GlyVal: 6.677 ± 0.724
2.636GlyTrp: 2.636 ± 0.396
2.372GlyTyr: 2.372 ± 0.351
0.0GlyXaa: 0.0 ± 0.0
His
2.46HisAla: 2.46 ± 0.332
0.132HisCys: 0.132 ± 0.074
1.054HisAsp: 1.054 ± 0.242
0.791HisGlu: 0.791 ± 0.192
0.923HisPhe: 0.923 ± 0.269
1.669HisGly: 1.669 ± 0.292
0.571HisHis: 0.571 ± 0.161
0.835HisIle: 0.835 ± 0.16
0.615HisLys: 0.615 ± 0.192
1.669HisLeu: 1.669 ± 0.336
0.351HisMet: 0.351 ± 0.099
0.747HisAsn: 0.747 ± 0.16
1.274HisPro: 1.274 ± 0.266
0.747HisGln: 0.747 ± 0.196
1.581HisArg: 1.581 ± 0.282
0.571HisSer: 0.571 ± 0.144
1.318HisThr: 1.318 ± 0.27
1.098HisVal: 1.098 ± 0.231
0.571HisTrp: 0.571 ± 0.152
0.703HisTyr: 0.703 ± 0.208
0.0HisXaa: 0.0 ± 0.0
Ile
6.326IleAla: 6.326 ± 0.494
0.439IleCys: 0.439 ± 0.134
3.646IleAsp: 3.646 ± 0.381
4.349IleGlu: 4.349 ± 0.473
0.879IlePhe: 0.879 ± 0.165
4.129IleGly: 4.129 ± 0.479
0.703IleHis: 0.703 ± 0.199
2.504IleIle: 2.504 ± 0.319
1.098IleLys: 1.098 ± 0.25
2.943IleLeu: 2.943 ± 0.33
0.395IleMet: 0.395 ± 0.148
1.669IleAsn: 1.669 ± 0.223
2.724IlePro: 2.724 ± 0.297
1.845IleGln: 1.845 ± 0.351
2.68IleArg: 2.68 ± 0.473
2.548IleSer: 2.548 ± 0.317
3.339IleThr: 3.339 ± 0.319
2.943IleVal: 2.943 ± 0.351
0.615IleTrp: 0.615 ± 0.138
0.615IleTyr: 0.615 ± 0.133
0.0IleXaa: 0.0 ± 0.0
Lys
4.437LysAla: 4.437 ± 0.63
0.308LysCys: 0.308 ± 0.123
1.186LysAsp: 1.186 ± 0.205
1.933LysGlu: 1.933 ± 0.289
0.351LysPhe: 0.351 ± 0.111
2.504LysGly: 2.504 ± 0.316
0.483LysHis: 0.483 ± 0.177
1.494LysIle: 1.494 ± 0.24
1.23LysLys: 1.23 ± 0.274
3.251LysLeu: 3.251 ± 0.397
0.615LysMet: 0.615 ± 0.166
0.879LysAsn: 0.879 ± 0.206
1.274LysPro: 1.274 ± 0.241
0.703LysGln: 0.703 ± 0.163
1.801LysArg: 1.801 ± 0.263
1.625LysSer: 1.625 ± 0.261
1.318LysThr: 1.318 ± 0.217
2.768LysVal: 2.768 ± 0.392
0.527LysTrp: 0.527 ± 0.134
0.615LysTyr: 0.615 ± 0.167
0.0LysXaa: 0.0 ± 0.0
Leu
12.476LeuAla: 12.476 ± 0.706
0.439LeuCys: 0.439 ± 0.162
6.062LeuAsp: 6.062 ± 0.603
4.305LeuGlu: 4.305 ± 0.409
1.977LeuPhe: 1.977 ± 0.264
7.995LeuGly: 7.995 ± 0.787
1.362LeuHis: 1.362 ± 0.264
3.47LeuIle: 3.47 ± 0.466
2.109LeuLys: 2.109 ± 0.429
6.106LeuLeu: 6.106 ± 0.474
2.768LeuMet: 2.768 ± 0.347
2.724LeuAsn: 2.724 ± 0.407
6.238LeuPro: 6.238 ± 0.548
2.724LeuGln: 2.724 ± 0.369
4.613LeuArg: 4.613 ± 0.407
4.92LeuSer: 4.92 ± 0.527
5.579LeuThr: 5.579 ± 0.524
5.315LeuVal: 5.315 ± 0.427
1.054LeuTrp: 1.054 ± 0.205
1.669LeuTyr: 1.669 ± 0.244
0.0LeuXaa: 0.0 ± 0.0
Met
3.778MetAla: 3.778 ± 0.325
0.264MetCys: 0.264 ± 0.096
1.362MetAsp: 1.362 ± 0.202
1.098MetGlu: 1.098 ± 0.291
0.966MetPhe: 0.966 ± 0.211
2.065MetGly: 2.065 ± 0.455
0.395MetHis: 0.395 ± 0.164
1.581MetIle: 1.581 ± 0.254
0.615MetLys: 0.615 ± 0.18
1.581MetLeu: 1.581 ± 0.254
0.527MetMet: 0.527 ± 0.147
0.527MetAsn: 0.527 ± 0.165
1.801MetPro: 1.801 ± 0.273
0.483MetGln: 0.483 ± 0.15
1.538MetArg: 1.538 ± 0.277
1.801MetSer: 1.801 ± 0.305
2.065MetThr: 2.065 ± 0.336
1.054MetVal: 1.054 ± 0.163
0.264MetTrp: 0.264 ± 0.1
0.395MetTyr: 0.395 ± 0.128
0.0MetXaa: 0.0 ± 0.0
Asn
4.305AsnAla: 4.305 ± 0.574
0.22AsnCys: 0.22 ± 0.089
1.45AsnAsp: 1.45 ± 0.255
1.098AsnGlu: 1.098 ± 0.247
0.395AsnPhe: 0.395 ± 0.138
2.724AsnGly: 2.724 ± 0.348
0.615AsnHis: 0.615 ± 0.169
1.186AsnIle: 1.186 ± 0.309
0.791AsnLys: 0.791 ± 0.203
2.46AsnLeu: 2.46 ± 0.437
0.747AsnMet: 0.747 ± 0.168
0.747AsnAsn: 0.747 ± 0.204
2.372AsnPro: 2.372 ± 0.357
0.395AsnGln: 0.395 ± 0.149
1.362AsnArg: 1.362 ± 0.255
1.186AsnSer: 1.186 ± 0.322
1.669AsnThr: 1.669 ± 0.261
1.757AsnVal: 1.757 ± 0.292
0.527AsnTrp: 0.527 ± 0.17
0.571AsnTyr: 0.571 ± 0.189
0.0AsnXaa: 0.0 ± 0.0
Pro
8.259ProAla: 8.259 ± 0.791
0.835ProCys: 0.835 ± 0.21
4.437ProAsp: 4.437 ± 0.494
5.535ProGlu: 5.535 ± 0.449
2.109ProPhe: 2.109 ± 0.284
7.468ProGly: 7.468 ± 0.685
0.923ProHis: 0.923 ± 0.221
2.592ProIle: 2.592 ± 0.321
1.538ProLys: 1.538 ± 0.237
4.085ProLeu: 4.085 ± 0.473
1.098ProMet: 1.098 ± 0.205
1.757ProAsn: 1.757 ± 0.295
4.876ProPro: 4.876 ± 0.646
1.669ProGln: 1.669 ± 0.243
2.768ProArg: 2.768 ± 0.391
3.558ProSer: 3.558 ± 0.564
4.261ProThr: 4.261 ± 0.468
4.393ProVal: 4.393 ± 0.395
1.274ProTrp: 1.274 ± 0.263
0.747ProTyr: 0.747 ± 0.224
0.0ProXaa: 0.0 ± 0.0
Gln
4.832GlnAla: 4.832 ± 0.638
0.044GlnCys: 0.044 ± 0.047
1.494GlnAsp: 1.494 ± 0.296
1.845GlnGlu: 1.845 ± 0.291
1.01GlnPhe: 1.01 ± 0.246
3.426GlnGly: 3.426 ± 0.442
0.527GlnHis: 0.527 ± 0.166
1.713GlnIle: 1.713 ± 0.232
0.659GlnLys: 0.659 ± 0.181
4.525GlnLeu: 4.525 ± 0.379
0.923GlnMet: 0.923 ± 0.186
0.659GlnAsn: 0.659 ± 0.21
2.416GlnPro: 2.416 ± 0.309
1.45GlnGln: 1.45 ± 0.618
2.811GlnArg: 2.811 ± 0.324
1.45GlnSer: 1.45 ± 0.248
1.801GlnThr: 1.801 ± 0.304
2.416GlnVal: 2.416 ± 0.307
0.527GlnTrp: 0.527 ± 0.155
0.791GlnTyr: 0.791 ± 0.167
0.0GlnXaa: 0.0 ± 0.0
Arg
9.005ArgAla: 9.005 ± 0.854
0.659ArgCys: 0.659 ± 0.164
3.47ArgAsp: 3.47 ± 0.398
5.14ArgGlu: 5.14 ± 0.51
1.538ArgPhe: 1.538 ± 0.229
4.393ArgGly: 4.393 ± 0.505
1.142ArgHis: 1.142 ± 0.263
2.855ArgIle: 2.855 ± 0.335
1.757ArgLys: 1.757 ± 0.313
6.106ArgLeu: 6.106 ± 0.76
2.153ArgMet: 2.153 ± 0.305
2.153ArgAsn: 2.153 ± 0.287
3.558ArgPro: 3.558 ± 0.444
2.636ArgGln: 2.636 ± 0.356
6.282ArgArg: 6.282 ± 0.687
3.602ArgSer: 3.602 ± 0.504
3.119ArgThr: 3.119 ± 0.388
4.305ArgVal: 4.305 ± 0.489
1.977ArgTrp: 1.977 ± 0.35
2.065ArgTyr: 2.065 ± 0.344
0.0ArgXaa: 0.0 ± 0.0
Ser
6.37SerAla: 6.37 ± 0.552
0.571SerCys: 0.571 ± 0.188
2.987SerAsp: 2.987 ± 0.433
2.811SerGlu: 2.811 ± 0.364
1.494SerPhe: 1.494 ± 0.261
6.985SerGly: 6.985 ± 0.776
0.835SerHis: 0.835 ± 0.154
2.372SerIle: 2.372 ± 0.309
2.24SerLys: 2.24 ± 0.337
4.129SerLeu: 4.129 ± 0.49
1.581SerMet: 1.581 ± 0.241
1.406SerAsn: 1.406 ± 0.315
3.031SerPro: 3.031 ± 0.335
2.899SerGln: 2.899 ± 0.323
3.383SerArg: 3.383 ± 0.409
2.504SerSer: 2.504 ± 0.355
2.196SerThr: 2.196 ± 0.303
3.119SerVal: 3.119 ± 0.358
1.581SerTrp: 1.581 ± 0.242
1.098SerTyr: 1.098 ± 0.289
0.0SerXaa: 0.0 ± 0.0
Thr
6.853ThrAla: 6.853 ± 0.628
0.483ThrCys: 0.483 ± 0.14
3.207ThrAsp: 3.207 ± 0.377
3.822ThrGlu: 3.822 ± 0.361
1.625ThrPhe: 1.625 ± 0.282
5.93ThrGly: 5.93 ± 0.5
0.835ThrHis: 0.835 ± 0.163
3.69ThrIle: 3.69 ± 0.438
1.406ThrLys: 1.406 ± 0.264
4.129ThrLeu: 4.129 ± 0.458
1.669ThrMet: 1.669 ± 0.278
1.362ThrAsn: 1.362 ± 0.256
4.173ThrPro: 4.173 ± 0.539
1.23ThrGln: 1.23 ± 0.216
3.558ThrArg: 3.558 ± 0.39
3.954ThrSer: 3.954 ± 0.448
2.987ThrThr: 2.987 ± 0.411
4.173ThrVal: 4.173 ± 0.416
1.23ThrTrp: 1.23 ± 0.253
1.098ThrTyr: 1.098 ± 0.174
0.0ThrXaa: 0.0 ± 0.0
Val
8.742ValAla: 8.742 ± 0.626
0.439ValCys: 0.439 ± 0.158
3.075ValAsp: 3.075 ± 0.488
4.393ValGlu: 4.393 ± 0.531
1.669ValPhe: 1.669 ± 0.245
6.194ValGly: 6.194 ± 0.496
1.713ValHis: 1.713 ± 0.287
3.558ValIle: 3.558 ± 0.317
2.196ValLys: 2.196 ± 0.31
5.974ValLeu: 5.974 ± 0.536
1.581ValMet: 1.581 ± 0.313
1.581ValAsn: 1.581 ± 0.302
4.129ValPro: 4.129 ± 0.347
2.416ValGln: 2.416 ± 0.412
4.656ValArg: 4.656 ± 0.544
3.251ValSer: 3.251 ± 0.4
5.008ValThr: 5.008 ± 0.543
3.91ValVal: 3.91 ± 0.378
1.186ValTrp: 1.186 ± 0.177
1.362ValTyr: 1.362 ± 0.212
0.0ValXaa: 0.0 ± 0.0
Trp
1.889TrpAla: 1.889 ± 0.319
0.571TrpCys: 0.571 ± 0.178
1.01TrpAsp: 1.01 ± 0.206
0.835TrpGlu: 0.835 ± 0.204
0.615TrpPhe: 0.615 ± 0.226
1.274TrpGly: 1.274 ± 0.219
0.791TrpHis: 0.791 ± 0.224
0.527TrpIle: 0.527 ± 0.162
0.22TrpLys: 0.22 ± 0.084
2.109TrpLeu: 2.109 ± 0.31
0.439TrpMet: 0.439 ± 0.125
0.483TrpAsn: 0.483 ± 0.12
1.142TrpPro: 1.142 ± 0.252
0.659TrpGln: 0.659 ± 0.189
1.669TrpArg: 1.669 ± 0.287
1.538TrpSer: 1.538 ± 0.251
1.318TrpThr: 1.318 ± 0.307
1.713TrpVal: 1.713 ± 0.299
0.351TrpTrp: 0.351 ± 0.128
0.439TrpTyr: 0.439 ± 0.137
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.592TyrAla: 2.592 ± 0.391
0.22TyrCys: 0.22 ± 0.093
1.494TyrAsp: 1.494 ± 0.219
1.274TyrGlu: 1.274 ± 0.197
0.615TyrPhe: 0.615 ± 0.167
1.757TyrGly: 1.757 ± 0.331
0.308TyrHis: 0.308 ± 0.135
0.659TyrIle: 0.659 ± 0.208
0.571TyrLys: 0.571 ± 0.147
2.109TyrLeu: 2.109 ± 0.288
0.264TyrMet: 0.264 ± 0.09
0.659TyrAsn: 0.659 ± 0.156
1.142TyrPro: 1.142 ± 0.18
1.142TyrGln: 1.142 ± 0.256
1.581TyrArg: 1.581 ± 0.315
0.966TyrSer: 0.966 ± 0.16
0.835TyrThr: 0.835 ± 0.158
1.45TyrVal: 1.45 ± 0.255
0.264TyrTrp: 0.264 ± 0.106
0.439TyrTyr: 0.439 ± 0.199
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 98 proteins (22765 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski