Penicillium expansum (Blue mold rot fungus)
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11060 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A2I7I2|A0A0A2I7I2_PENEN Fatty acyl-CoA reductase OS=Penicillium expansum OX=27334 GN=PEX2_006590 PE=3 SV=1
MM1 pKa = 7.72 RR2 pKa = 11.84 TQQLLPLCGLLLASVAIASKK22 pKa = 10.51 LDD24 pKa = 3.57 HH25 pKa = 7.65 DD26 pKa = 5.06 DD27 pKa = 3.52 VPNRR31 pKa = 11.84 CWTVCSSVVDD41 pKa = 3.75 ISKK44 pKa = 10.6 SCDD47 pKa = 3.14 TKK49 pKa = 10.99 HH50 pKa = 6.14 EE51 pKa = 4.3 RR52 pKa = 11.84 DD53 pKa = 3.58 SAEE56 pKa = 3.92 IQCICDD62 pKa = 2.94 WDD64 pKa = 3.86 AAQTQIPLCSACITQYY80 pKa = 9.28 QTDD83 pKa = 3.44 RR84 pKa = 11.84 RR85 pKa = 11.84 NRR87 pKa = 11.84 NITQHH92 pKa = 7.27 DD93 pKa = 4.48 DD94 pKa = 4.73 DD95 pKa = 7.49 DD96 pKa = 7.53 DD97 pKa = 7.47 DD98 pKa = 7.65 DD99 pKa = 7.62 DD100 pKa = 7.63 DD101 pKa = 7.63 DD102 pKa = 7.57 DD103 pKa = 6.61 DD104 pKa = 6.6 DD105 pKa = 6.14 NEE107 pKa = 4.5 ALDD110 pKa = 4.81 LVHH113 pKa = 6.72 SCSLTTTTYY122 pKa = 11.04 NSAAATTVISTSTTTAGSNTATTTDD147 pKa = 3.15 SSSTSGTNSQDD158 pKa = 3.21 STSSVSSGSSDD169 pKa = 3.26 SAGASSGSASSPTPTPGAAVGISAPGAASMAVVMGLMAFAWLL211 pKa = 3.69
Molecular weight: 21.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.679
IPC2_protein 3.795
IPC_protein 3.834
Toseland 3.579
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.846
Rodwell 3.656
Grimsley 3.49
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.317
Thurlkill 3.668
EMBOSS 3.846
Sillero 3.961
Patrickios 1.138
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.84
Protein with the highest isoelectric point:
>tr|A0A0A2JE85|A0A0A2JE85_PENEN Uncharacterized protein OS=Penicillium expansum OX=27334 GN=PEX2_063050 PE=4 SV=1
MM1 pKa = 7.43 LSSRR5 pKa = 11.84 SFSSLLSTPTRR16 pKa = 11.84 FQPSRR21 pKa = 11.84 TLGARR26 pKa = 11.84 LSSPTTTATASAFSPLSSLLSQKK49 pKa = 10.58 APSQSRR55 pKa = 11.84 SFSASASLGVRR66 pKa = 11.84 RR67 pKa = 11.84 VTFRR71 pKa = 11.84 PSRR74 pKa = 11.84 RR75 pKa = 11.84 VQKK78 pKa = 10.3 RR79 pKa = 11.84 RR80 pKa = 11.84 HH81 pKa = 5.75 GYY83 pKa = 9.09 LARR86 pKa = 11.84 KK87 pKa = 9.08 KK88 pKa = 10.39 DD89 pKa = 3.6 RR90 pKa = 11.84 NGRR93 pKa = 11.84 KK94 pKa = 6.83 TLIRR98 pKa = 11.84 RR99 pKa = 11.84 TLKK102 pKa = 10.34 GRR104 pKa = 11.84 KK105 pKa = 7.8 EE106 pKa = 4.02 LSWW109 pKa = 4.66
Molecular weight: 12.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.378
IPC2_protein 10.906
IPC_protein 12.384
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.223
Grimsley 12.588
Solomon 13.042
Lehninger 12.954
Nozaki 12.544
DTASelect 12.544
Thurlkill 12.544
EMBOSS 13.056
Sillero 12.544
Patrickios 11.945
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.088
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11060
0
11060
5559105
40
7287
502.6
55.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.403 ± 0.021
1.197 ± 0.008
5.649 ± 0.016
6.134 ± 0.023
3.862 ± 0.014
6.784 ± 0.023
2.425 ± 0.01
5.232 ± 0.016
4.635 ± 0.019
9.047 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.249 ± 0.008
3.777 ± 0.011
5.956 ± 0.029
4.045 ± 0.016
5.799 ± 0.017
8.323 ± 0.024
6.026 ± 0.014
6.164 ± 0.017
1.491 ± 0.008
2.801 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here