Pectobacterium phage PP90

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Corkvirinae; Phimunavirus; Pectobacterium virus PP90

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1PEH7|A0A1B1PEH7_9CAUD Putative DNA primase OS=Pectobacterium phage PP90 OX=1873959 GN=BI050_gp12 PE=4 SV=1
MM1 pKa = 7.23TKK3 pKa = 10.51YY4 pKa = 9.86IVSVPFCGYY13 pKa = 7.85MRR15 pKa = 11.84GYY17 pKa = 10.53KK18 pKa = 10.03VYY20 pKa = 9.52TVEE23 pKa = 4.36ADD25 pKa = 3.48SPEE28 pKa = 4.07EE29 pKa = 4.0AKK31 pKa = 10.76EE32 pKa = 4.07KK33 pKa = 10.73ACDD36 pKa = 3.1WDD38 pKa = 4.48YY39 pKa = 10.37ITEE42 pKa = 4.55SIEE45 pKa = 3.82VTRR48 pKa = 11.84DD49 pKa = 3.17DD50 pKa = 6.12SEE52 pKa = 4.54TDD54 pKa = 2.87WANAEE59 pKa = 4.24VAHH62 pKa = 6.83DD63 pKa = 3.78

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1PEI2|A0A1B1PEI2_9CAUD Uncharacterized protein OS=Pectobacterium phage PP90 OX=1873959 GN=BI050_gp33 PE=4 SV=1
MM1 pKa = 7.54SCTDD5 pKa = 3.34HH6 pKa = 6.75GRR8 pKa = 11.84HH9 pKa = 6.01KK10 pKa = 10.78SLSKK14 pKa = 10.62EE15 pKa = 3.95GYY17 pKa = 10.51LLVKK21 pKa = 10.44DD22 pKa = 3.82PRR24 pKa = 11.84KK25 pKa = 9.98KK26 pKa = 10.32SRR28 pKa = 11.84CTSLHH33 pKa = 5.69RR34 pKa = 11.84MVYY37 pKa = 8.17ACKK40 pKa = 10.01MGTNLDD46 pKa = 4.2GIADD50 pKa = 3.6VVVRR54 pKa = 11.84HH55 pKa = 5.64TCDD58 pKa = 2.86NPRR61 pKa = 11.84CINPEE66 pKa = 3.87HH67 pKa = 7.01LIGGTKK73 pKa = 10.15ADD75 pKa = 3.81NNRR78 pKa = 11.84DD79 pKa = 3.16RR80 pKa = 11.84AEE82 pKa = 4.18RR83 pKa = 11.84GRR85 pKa = 11.84SARR88 pKa = 11.84VVPSRR93 pKa = 11.84QRR95 pKa = 11.84LSQEE99 pKa = 3.57DD100 pKa = 3.82VQFIRR105 pKa = 11.84LNWVKK110 pKa = 10.38RR111 pKa = 11.84HH112 pKa = 5.88PLFGTTKK119 pKa = 8.54LAARR123 pKa = 11.84FGVDD127 pKa = 2.68SAVIIRR133 pKa = 11.84VIKK136 pKa = 8.84GTYY139 pKa = 8.12VCTRR143 pKa = 3.33

Molecular weight: 16.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

13911

39

1263

248.4

27.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.59 ± 0.413

1.222 ± 0.176

6.146 ± 0.234

5.255 ± 0.303

3.019 ± 0.124

7.613 ± 0.25

2.042 ± 0.202

4.471 ± 0.152

4.658 ± 0.308

8.648 ± 0.224

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.638 ± 0.181

4.615 ± 0.251

3.99 ± 0.189

4.903 ± 0.347

5.528 ± 0.281

6.599 ± 0.258

6.362 ± 0.425

7.354 ± 0.22

1.387 ± 0.136

3.961 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski