Amino acid dipepetide frequency for Pectobacterium phage PP90

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.365AlaAla: 12.365 ± 1.318
1.15AlaCys: 1.15 ± 0.325
5.464AlaAsp: 5.464 ± 0.611
5.679AlaGlu: 5.679 ± 0.894
3.091AlaPhe: 3.091 ± 0.525
6.254AlaGly: 6.254 ± 0.679
2.085AlaHis: 2.085 ± 0.553
2.66AlaIle: 2.66 ± 0.374
4.817AlaLys: 4.817 ± 0.588
8.699AlaLeu: 8.699 ± 0.838
2.66AlaMet: 2.66 ± 0.47
3.882AlaAsn: 3.882 ± 0.564
3.738AlaPro: 3.738 ± 0.541
5.464AlaGln: 5.464 ± 0.793
4.889AlaArg: 4.889 ± 0.648
6.254AlaSer: 6.254 ± 0.836
4.745AlaThr: 4.745 ± 0.64
7.261AlaVal: 7.261 ± 0.709
1.366AlaTrp: 1.366 ± 0.33
3.307AlaTyr: 3.307 ± 0.545
0.0AlaXaa: 0.0 ± 0.0
Cys
0.575CysAla: 0.575 ± 0.238
0.431CysCys: 0.431 ± 0.18
0.791CysAsp: 0.791 ± 0.25
0.359CysGlu: 0.359 ± 0.154
0.503CysPhe: 0.503 ± 0.209
0.935CysGly: 0.935 ± 0.257
0.647CysHis: 0.647 ± 0.245
1.006CysIle: 1.006 ± 0.335
0.216CysLys: 0.216 ± 0.114
0.935CysLeu: 0.935 ± 0.283
0.575CysMet: 0.575 ± 0.226
0.575CysAsn: 0.575 ± 0.225
0.431CysPro: 0.431 ± 0.227
0.288CysGln: 0.288 ± 0.148
0.647CysArg: 0.647 ± 0.267
0.935CysSer: 0.935 ± 0.315
1.078CysThr: 1.078 ± 0.373
0.647CysVal: 0.647 ± 0.218
0.216CysTrp: 0.216 ± 0.115
0.431CysTyr: 0.431 ± 0.144
0.0CysXaa: 0.0 ± 0.0
Asp
6.111AspAla: 6.111 ± 0.749
0.503AspCys: 0.503 ± 0.226
4.17AspAsp: 4.17 ± 0.646
3.163AspGlu: 3.163 ± 0.449
1.797AspPhe: 1.797 ± 0.239
4.601AspGly: 4.601 ± 0.599
0.863AspHis: 0.863 ± 0.295
3.882AspIle: 3.882 ± 0.436
2.588AspLys: 2.588 ± 0.574
5.104AspLeu: 5.104 ± 0.647
2.301AspMet: 2.301 ± 0.397
2.804AspAsn: 2.804 ± 0.411
2.013AspPro: 2.013 ± 0.302
1.222AspGln: 1.222 ± 0.343
3.019AspArg: 3.019 ± 0.57
4.673AspSer: 4.673 ± 0.578
4.242AspThr: 4.242 ± 0.538
4.673AspVal: 4.673 ± 0.484
1.582AspTrp: 1.582 ± 0.3
2.157AspTyr: 2.157 ± 0.477
0.0AspXaa: 0.0 ± 0.0
Glu
5.248GluAla: 5.248 ± 0.641
0.431GluCys: 0.431 ± 0.203
3.81GluAsp: 3.81 ± 0.616
3.307GluGlu: 3.307 ± 0.715
2.588GluPhe: 2.588 ± 0.439
2.732GluGly: 2.732 ± 0.439
1.366GluHis: 1.366 ± 0.381
2.013GluIle: 2.013 ± 0.386
2.732GluLys: 2.732 ± 0.572
5.32GluLeu: 5.32 ± 0.57
1.51GluMet: 1.51 ± 0.31
2.013GluAsn: 2.013 ± 0.357
1.222GluPro: 1.222 ± 0.291
3.666GluGln: 3.666 ± 0.452
2.229GluArg: 2.229 ± 0.455
2.804GluSer: 2.804 ± 0.466
2.804GluThr: 2.804 ± 0.588
3.595GluVal: 3.595 ± 0.631
0.647GluTrp: 0.647 ± 0.261
2.516GluTyr: 2.516 ± 0.434
0.0GluXaa: 0.0 ± 0.0
Phe
2.516PheAla: 2.516 ± 0.458
0.216PheCys: 0.216 ± 0.123
2.516PheAsp: 2.516 ± 0.426
1.222PheGlu: 1.222 ± 0.215
1.006PhePhe: 1.006 ± 0.271
2.804PheGly: 2.804 ± 0.45
0.503PheHis: 0.503 ± 0.217
1.869PheIle: 1.869 ± 0.29
1.653PheLys: 1.653 ± 0.349
2.157PheLeu: 2.157 ± 0.408
0.503PheMet: 0.503 ± 0.167
2.301PheAsn: 2.301 ± 0.506
1.222PhePro: 1.222 ± 0.282
1.582PheGln: 1.582 ± 0.285
1.51PheArg: 1.51 ± 0.343
1.653PheSer: 1.653 ± 0.358
1.51PheThr: 1.51 ± 0.397
2.229PheVal: 2.229 ± 0.416
0.431PheTrp: 0.431 ± 0.163
0.791PheTyr: 0.791 ± 0.289
0.0PheXaa: 0.0 ± 0.0
Gly
6.83GlyAla: 6.83 ± 0.791
1.222GlyCys: 1.222 ± 0.456
4.529GlyAsp: 4.529 ± 0.675
3.307GlyGlu: 3.307 ± 0.516
2.516GlyPhe: 2.516 ± 0.35
5.32GlyGly: 5.32 ± 0.712
0.647GlyHis: 0.647 ± 0.226
4.817GlyIle: 4.817 ± 0.623
3.81GlyLys: 3.81 ± 0.527
6.47GlyLeu: 6.47 ± 0.656
1.797GlyMet: 1.797 ± 0.335
4.098GlyAsn: 4.098 ± 0.69
1.294GlyPro: 1.294 ± 0.347
2.444GlyGln: 2.444 ± 0.45
3.738GlyArg: 3.738 ± 0.436
5.32GlySer: 5.32 ± 0.582
7.261GlyThr: 7.261 ± 0.631
5.967GlyVal: 5.967 ± 0.733
0.935GlyTrp: 0.935 ± 0.236
3.81GlyTyr: 3.81 ± 0.541
0.0GlyXaa: 0.0 ± 0.0
His
1.725HisAla: 1.725 ± 0.452
0.503HisCys: 0.503 ± 0.189
1.006HisAsp: 1.006 ± 0.316
1.15HisGlu: 1.15 ± 0.37
0.216HisPhe: 0.216 ± 0.123
1.653HisGly: 1.653 ± 0.366
0.503HisHis: 0.503 ± 0.211
1.006HisIle: 1.006 ± 0.316
0.935HisLys: 0.935 ± 0.355
2.085HisLeu: 2.085 ± 0.432
0.575HisMet: 0.575 ± 0.265
1.006HisAsn: 1.006 ± 0.267
1.15HisPro: 1.15 ± 0.344
0.863HisGln: 0.863 ± 0.24
1.006HisArg: 1.006 ± 0.229
1.078HisSer: 1.078 ± 0.304
0.791HisThr: 0.791 ± 0.29
1.653HisVal: 1.653 ± 0.343
0.503HisTrp: 0.503 ± 0.177
1.006HisTyr: 1.006 ± 0.348
0.0HisXaa: 0.0 ± 0.0
Ile
3.882IleAla: 3.882 ± 0.601
0.647IleCys: 0.647 ± 0.243
3.451IleAsp: 3.451 ± 0.493
2.732IleGlu: 2.732 ± 0.462
0.863IlePhe: 0.863 ± 0.239
2.876IleGly: 2.876 ± 0.4
0.647IleHis: 0.647 ± 0.169
2.085IleIle: 2.085 ± 0.401
3.163IleLys: 3.163 ± 0.423
3.738IleLeu: 3.738 ± 0.543
1.222IleMet: 1.222 ± 0.31
2.732IleAsn: 2.732 ± 0.569
2.013IlePro: 2.013 ± 0.293
2.157IleGln: 2.157 ± 0.335
1.725IleArg: 1.725 ± 0.342
2.66IleSer: 2.66 ± 0.358
4.026IleThr: 4.026 ± 0.641
2.516IleVal: 2.516 ± 0.384
0.647IleTrp: 0.647 ± 0.252
0.935IleTyr: 0.935 ± 0.23
0.0IleXaa: 0.0 ± 0.0
Lys
5.104LysAla: 5.104 ± 0.865
0.359LysCys: 0.359 ± 0.162
3.379LysAsp: 3.379 ± 0.447
3.523LysGlu: 3.523 ± 0.691
0.791LysPhe: 0.791 ± 0.227
3.523LysGly: 3.523 ± 0.462
1.006LysHis: 1.006 ± 0.291
1.294LysIle: 1.294 ± 0.288
1.869LysLys: 1.869 ± 0.464
4.817LysLeu: 4.817 ± 0.697
1.294LysMet: 1.294 ± 0.392
1.366LysAsn: 1.366 ± 0.392
1.941LysPro: 1.941 ± 0.311
2.588LysGln: 2.588 ± 0.507
3.307LysArg: 3.307 ± 0.586
2.948LysSer: 2.948 ± 0.473
1.725LysThr: 1.725 ± 0.353
3.019LysVal: 3.019 ± 0.493
0.431LysTrp: 0.431 ± 0.174
2.301LysTyr: 2.301 ± 0.489
0.0LysXaa: 0.0 ± 0.0
Leu
7.836LeuAla: 7.836 ± 0.673
1.078LeuCys: 1.078 ± 0.31
4.529LeuAsp: 4.529 ± 0.618
4.385LeuGlu: 4.385 ± 0.648
2.516LeuPhe: 2.516 ± 0.448
6.398LeuGly: 6.398 ± 0.813
2.301LeuHis: 2.301 ± 0.462
3.019LeuIle: 3.019 ± 0.529
3.666LeuLys: 3.666 ± 0.566
7.477LeuLeu: 7.477 ± 0.777
2.229LeuMet: 2.229 ± 0.294
4.673LeuAsn: 4.673 ± 0.589
4.601LeuPro: 4.601 ± 0.516
3.81LeuGln: 3.81 ± 0.614
5.392LeuArg: 5.392 ± 0.807
6.686LeuSer: 6.686 ± 0.629
5.392LeuThr: 5.392 ± 0.823
6.542LeuVal: 6.542 ± 0.513
0.719LeuTrp: 0.719 ± 0.257
3.235LeuTyr: 3.235 ± 0.441
0.0LeuXaa: 0.0 ± 0.0
Met
2.444MetAla: 2.444 ± 0.419
0.288MetCys: 0.288 ± 0.142
1.222MetAsp: 1.222 ± 0.255
0.863MetGlu: 0.863 ± 0.268
1.15MetPhe: 1.15 ± 0.275
2.301MetGly: 2.301 ± 0.448
0.431MetHis: 0.431 ± 0.163
0.719MetIle: 0.719 ± 0.214
0.935MetLys: 0.935 ± 0.255
2.229MetLeu: 2.229 ± 0.465
0.503MetMet: 0.503 ± 0.166
1.006MetAsn: 1.006 ± 0.224
1.366MetPro: 1.366 ± 0.392
2.013MetGln: 2.013 ± 0.444
2.085MetArg: 2.085 ± 0.527
2.085MetSer: 2.085 ± 0.473
1.51MetThr: 1.51 ± 0.305
1.797MetVal: 1.797 ± 0.34
0.144MetTrp: 0.144 ± 0.097
1.294MetTyr: 1.294 ± 0.315
0.0MetXaa: 0.0 ± 0.0
Asn
3.523AsnAla: 3.523 ± 0.524
0.791AsnCys: 0.791 ± 0.293
2.085AsnAsp: 2.085 ± 0.331
2.157AsnGlu: 2.157 ± 0.438
1.222AsnPhe: 1.222 ± 0.342
4.026AsnGly: 4.026 ± 0.475
0.791AsnHis: 0.791 ± 0.261
1.582AsnIle: 1.582 ± 0.346
3.019AsnLys: 3.019 ± 0.413
4.817AsnLeu: 4.817 ± 0.667
1.366AsnMet: 1.366 ± 0.277
2.229AsnAsn: 2.229 ± 0.41
2.588AsnPro: 2.588 ± 0.423
2.229AsnGln: 2.229 ± 0.408
2.301AsnArg: 2.301 ± 0.435
3.163AsnSer: 3.163 ± 0.688
3.738AsnThr: 3.738 ± 0.6
2.804AsnVal: 2.804 ± 0.512
0.719AsnTrp: 0.719 ± 0.22
1.006AsnTyr: 1.006 ± 0.298
0.0AsnXaa: 0.0 ± 0.0
Pro
3.954ProAla: 3.954 ± 0.538
0.216ProCys: 0.216 ± 0.13
3.954ProAsp: 3.954 ± 0.519
3.451ProGlu: 3.451 ± 0.508
0.791ProPhe: 0.791 ± 0.24
2.588ProGly: 2.588 ± 0.351
0.431ProHis: 0.431 ± 0.156
1.438ProIle: 1.438 ± 0.336
1.582ProLys: 1.582 ± 0.365
2.588ProLeu: 2.588 ± 0.483
0.935ProMet: 0.935 ± 0.239
1.294ProAsn: 1.294 ± 0.329
1.51ProPro: 1.51 ± 0.329
1.51ProGln: 1.51 ± 0.328
1.797ProArg: 1.797 ± 0.321
2.732ProSer: 2.732 ± 0.403
2.948ProThr: 2.948 ± 0.452
3.81ProVal: 3.81 ± 0.49
0.863ProTrp: 0.863 ± 0.28
1.51ProTyr: 1.51 ± 0.268
0.0ProXaa: 0.0 ± 0.0
Gln
6.183GlnAla: 6.183 ± 0.752
0.431GlnCys: 0.431 ± 0.153
2.444GlnAsp: 2.444 ± 0.414
2.301GlnGlu: 2.301 ± 0.395
2.013GlnPhe: 2.013 ± 0.342
3.954GlnGly: 3.954 ± 0.603
1.438GlnHis: 1.438 ± 0.301
1.653GlnIle: 1.653 ± 0.369
2.013GlnLys: 2.013 ± 0.396
3.954GlnLeu: 3.954 ± 0.667
1.15GlnMet: 1.15 ± 0.249
2.301GlnAsn: 2.301 ± 0.552
1.582GlnPro: 1.582 ± 0.328
3.379GlnGln: 3.379 ± 0.742
2.804GlnArg: 2.804 ± 0.448
2.66GlnSer: 2.66 ± 0.546
2.013GlnThr: 2.013 ± 0.406
3.307GlnVal: 3.307 ± 0.524
0.359GlnTrp: 0.359 ± 0.156
3.091GlnTyr: 3.091 ± 0.461
0.0GlnXaa: 0.0 ± 0.0
Arg
4.026ArgAla: 4.026 ± 0.436
1.078ArgCys: 1.078 ± 0.35
3.379ArgAsp: 3.379 ± 0.457
2.948ArgGlu: 2.948 ± 0.397
1.438ArgPhe: 1.438 ± 0.297
4.026ArgGly: 4.026 ± 0.537
1.438ArgHis: 1.438 ± 0.363
3.379ArgIle: 3.379 ± 0.573
3.019ArgLys: 3.019 ± 0.497
3.954ArgLeu: 3.954 ± 0.578
1.51ArgMet: 1.51 ± 0.407
2.588ArgAsn: 2.588 ± 0.539
1.51ArgPro: 1.51 ± 0.343
2.229ArgGln: 2.229 ± 0.437
4.026ArgArg: 4.026 ± 0.47
3.523ArgSer: 3.523 ± 0.74
3.019ArgThr: 3.019 ± 0.587
4.745ArgVal: 4.745 ± 0.613
1.006ArgTrp: 1.006 ± 0.25
2.444ArgTyr: 2.444 ± 0.475
0.0ArgXaa: 0.0 ± 0.0
Ser
7.405SerAla: 7.405 ± 0.955
0.575SerCys: 0.575 ± 0.177
3.091SerAsp: 3.091 ± 0.507
2.372SerGlu: 2.372 ± 0.391
1.941SerPhe: 1.941 ± 0.361
5.967SerGly: 5.967 ± 0.894
1.15SerHis: 1.15 ± 0.377
4.17SerIle: 4.17 ± 0.816
3.379SerLys: 3.379 ± 0.567
5.679SerLeu: 5.679 ± 0.636
1.941SerMet: 1.941 ± 0.437
2.876SerAsn: 2.876 ± 0.518
2.157SerPro: 2.157 ± 0.386
2.444SerGln: 2.444 ± 0.411
3.235SerArg: 3.235 ± 0.432
3.954SerSer: 3.954 ± 0.466
4.889SerThr: 4.889 ± 0.783
5.895SerVal: 5.895 ± 0.56
1.15SerTrp: 1.15 ± 0.329
1.725SerTyr: 1.725 ± 0.31
0.0SerXaa: 0.0 ± 0.0
Thr
6.542ThrAla: 6.542 ± 0.811
0.503ThrCys: 0.503 ± 0.222
3.451ThrAsp: 3.451 ± 0.531
3.595ThrGlu: 3.595 ± 0.437
1.438ThrPhe: 1.438 ± 0.268
6.542ThrGly: 6.542 ± 0.941
1.653ThrHis: 1.653 ± 0.304
2.229ThrIle: 2.229 ± 0.337
2.948ThrLys: 2.948 ± 0.417
5.032ThrLeu: 5.032 ± 0.539
1.078ThrMet: 1.078 ± 0.253
2.948ThrAsn: 2.948 ± 0.492
3.666ThrPro: 3.666 ± 0.687
2.013ThrGln: 2.013 ± 0.483
2.948ThrArg: 2.948 ± 0.57
5.032ThrSer: 5.032 ± 0.607
4.313ThrThr: 4.313 ± 0.942
4.96ThrVal: 4.96 ± 0.735
0.431ThrTrp: 0.431 ± 0.185
2.301ThrTyr: 2.301 ± 0.342
0.0ThrXaa: 0.0 ± 0.0
Val
6.039ValAla: 6.039 ± 0.504
1.078ValCys: 1.078 ± 0.317
4.601ValAsp: 4.601 ± 0.722
3.019ValGlu: 3.019 ± 0.473
2.372ValPhe: 2.372 ± 0.324
5.464ValGly: 5.464 ± 0.594
1.653ValHis: 1.653 ± 0.369
3.235ValIle: 3.235 ± 0.538
2.588ValLys: 2.588 ± 0.548
6.758ValLeu: 6.758 ± 0.876
1.653ValMet: 1.653 ± 0.331
3.019ValAsn: 3.019 ± 0.547
3.738ValPro: 3.738 ± 0.472
6.254ValGln: 6.254 ± 0.627
4.817ValArg: 4.817 ± 0.59
4.385ValSer: 4.385 ± 0.525
4.385ValThr: 4.385 ± 0.648
4.673ValVal: 4.673 ± 0.619
0.791ValTrp: 0.791 ± 0.248
3.019ValTyr: 3.019 ± 0.389
0.0ValXaa: 0.0 ± 0.0
Trp
0.935TrpAla: 0.935 ± 0.248
0.144TrpCys: 0.144 ± 0.105
0.935TrpAsp: 0.935 ± 0.311
0.935TrpGlu: 0.935 ± 0.29
0.935TrpPhe: 0.935 ± 0.311
1.078TrpGly: 1.078 ± 0.346
0.072TrpHis: 0.072 ± 0.069
0.288TrpIle: 0.288 ± 0.159
0.144TrpLys: 0.144 ± 0.107
1.366TrpLeu: 1.366 ± 0.377
0.431TrpMet: 0.431 ± 0.167
0.719TrpAsn: 0.719 ± 0.252
0.431TrpPro: 0.431 ± 0.186
0.719TrpGln: 0.719 ± 0.241
0.719TrpArg: 0.719 ± 0.244
0.863TrpSer: 0.863 ± 0.287
0.575TrpThr: 0.575 ± 0.188
1.294TrpVal: 1.294 ± 0.275
0.288TrpTrp: 0.288 ± 0.163
1.006TrpTyr: 1.006 ± 0.322
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.66TyrAla: 2.66 ± 0.393
0.575TyrCys: 0.575 ± 0.234
2.66TyrAsp: 2.66 ± 0.369
2.085TyrGlu: 2.085 ± 0.416
1.006TyrPhe: 1.006 ± 0.27
3.091TyrGly: 3.091 ± 0.578
0.791TyrHis: 0.791 ± 0.192
2.372TyrIle: 2.372 ± 0.487
1.51TyrLys: 1.51 ± 0.365
3.307TyrLeu: 3.307 ± 0.579
1.078TyrMet: 1.078 ± 0.309
1.725TyrAsn: 1.725 ± 0.366
1.725TyrPro: 1.725 ± 0.289
2.157TyrGln: 2.157 ± 0.361
3.091TyrArg: 3.091 ± 0.436
2.588TyrSer: 2.588 ± 0.441
2.66TyrThr: 2.66 ± 0.449
2.157TyrVal: 2.157 ± 0.347
0.647TyrTrp: 0.647 ± 0.312
1.725TyrTyr: 1.725 ± 0.46
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (13911 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski