Chondrostereum purpureum cryptic virus 1
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B7ZGY9|B7ZGY9_9VIRU Coat protein OS=Chondrostereum purpureum cryptic virus 1 OX=529380 GN=CP PE=4 SV=1
MM1 pKa = 7.34 SLSSGPVSQAVLTASEE17 pKa = 4.38 PAVPAPVEE25 pKa = 4.06 YY26 pKa = 10.36 RR27 pKa = 11.84 PGATQPDD34 pKa = 3.79 ATGTMTQTASGTANAIFQSGATTAPEE60 pKa = 3.83 QPPVLIHH67 pKa = 6.52 VSDD70 pKa = 4.08 DD71 pKa = 3.41 AKK73 pKa = 11.44 SNEE76 pKa = 4.06 KK77 pKa = 9.91 MSAIDD82 pKa = 3.05 ITRR85 pKa = 11.84 IAICYY90 pKa = 8.87 PMSTDD95 pKa = 3.48 SVLVPSWFLPSSRR108 pKa = 11.84 YY109 pKa = 9.57 LYY111 pKa = 10.4 RR112 pKa = 11.84 IVQEE116 pKa = 3.91 MDD118 pKa = 2.87 IIMLNTHH125 pKa = 7.15 RR126 pKa = 11.84 FTQSVHH132 pKa = 4.79 AWSPLVSRR140 pKa = 11.84 VYY142 pKa = 10.2 IAVLFWIQTIRR153 pKa = 11.84 CMQATGFLPRR163 pKa = 11.84 EE164 pKa = 4.46 GIAFINEE171 pKa = 4.22 LLQDD175 pKa = 4.3 FPARR179 pKa = 11.84 TLVVPGPLVSLFRR192 pKa = 11.84 SLCASSPSFGTYY204 pKa = 10.45 GDD206 pKa = 3.7 VFPAFEE212 pKa = 4.12 TAGIGASPANCFTLNSARR230 pKa = 11.84 FPQWYY235 pKa = 8.37 MLMPNVALLLDD246 pKa = 3.64 QLTCFTQACVNNPATIGDD264 pKa = 4.02 FVIGDD269 pKa = 4.34 DD270 pKa = 4.91 LFGTPFDD277 pKa = 3.93 GTIGSHH283 pKa = 5.01 RR284 pKa = 11.84 QQRR287 pKa = 11.84 VAPGMTEE294 pKa = 3.4 PVYY297 pKa = 10.86 FNTKK301 pKa = 9.35 AAEE304 pKa = 3.98 NHH306 pKa = 5.36 ASMRR310 pKa = 11.84 NLMALPTRR318 pKa = 11.84 LNYY321 pKa = 10.38 DD322 pKa = 3.53 PQNATPLTLNEE333 pKa = 4.02 MFRR336 pKa = 11.84 FNTDD340 pKa = 2.41 KK341 pKa = 10.85 RR342 pKa = 11.84 WFSRR346 pKa = 11.84 IISTMTVYY354 pKa = 10.95 SKK356 pKa = 10.97 FFDD359 pKa = 4.15 GSVTLYY365 pKa = 9.12 EE366 pKa = 4.82 CPPVGPPAAQVSMVRR381 pKa = 11.84 TQPMRR386 pKa = 11.84 FEE388 pKa = 4.76 ASNTAQAMEE397 pKa = 4.38 TEE399 pKa = 4.69 SFEE402 pKa = 4.21 STAISVNRR410 pKa = 11.84 SLSLHH415 pKa = 6.02 DD416 pKa = 4.26 EE417 pKa = 4.32 KK418 pKa = 11.52 VALAALINEE427 pKa = 4.61 TLVSTGDD434 pKa = 3.46 QPIYY438 pKa = 10.06 AAQRR442 pKa = 11.84 LGPFWNISPTRR453 pKa = 11.84 RR454 pKa = 11.84 QAYY457 pKa = 9.4 RR458 pKa = 11.84 FDD460 pKa = 3.87 PSFVLPSVIRR470 pKa = 11.84 DD471 pKa = 3.75 HH472 pKa = 6.79 YY473 pKa = 11.71 ALTTATRR480 pKa = 3.54
Molecular weight: 52.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.543
IPC2_protein 5.601
IPC_protein 5.601
Toseland 5.893
ProMoST 5.919
Dawson 5.817
Bjellqvist 5.842
Wikipedia 5.804
Rodwell 5.779
Grimsley 5.995
Solomon 5.817
Lehninger 5.804
Nozaki 6.059
DTASelect 6.237
Thurlkill 6.198
EMBOSS 6.173
Sillero 6.148
Patrickios 3.897
IPC_peptide 5.83
IPC2_peptide 6.16
IPC2.peptide.svr19 6.107
Protein with the highest isoelectric point:
>tr|B7ZGY9|B7ZGY9_9VIRU Coat protein OS=Chondrostereum purpureum cryptic virus 1 OX=529380 GN=CP PE=4 SV=1
MM1 pKa = 7.95 RR2 pKa = 11.84 FTEE5 pKa = 4.44 YY6 pKa = 10.19 ILSGFKK12 pKa = 10.84 VLDD15 pKa = 3.48 SSMDD19 pKa = 3.13 RR20 pKa = 11.84 SRR22 pKa = 11.84 NYY24 pKa = 10.46 QFIGFDD30 pKa = 3.61 KK31 pKa = 11.0 PEE33 pKa = 4.25 SQPPADD39 pKa = 3.71 ATMHH43 pKa = 6.5 HH44 pKa = 7.03 AVQAPVKK51 pKa = 9.59 HH52 pKa = 6.15 AMRR55 pKa = 11.84 KK56 pKa = 8.85 ILLHH60 pKa = 6.22 NEE62 pKa = 4.01 FVHH65 pKa = 5.8 ITTKK69 pKa = 10.66 FKK71 pKa = 10.87 RR72 pKa = 11.84 STIDD76 pKa = 3.22 DD77 pKa = 3.74 SSITEE82 pKa = 4.15 AFFKK86 pKa = 11.09 GDD88 pKa = 3.57 LPYY91 pKa = 10.99 HH92 pKa = 6.38 KK93 pKa = 10.26 VPKK96 pKa = 10.19 DD97 pKa = 3.36 GSYY100 pKa = 10.8 QRR102 pKa = 11.84 ALAATMQVFAPQVPIKK118 pKa = 9.72 PVHH121 pKa = 5.6 YY122 pKa = 9.95 CDD124 pKa = 4.32 LRR126 pKa = 11.84 YY127 pKa = 10.18 YY128 pKa = 9.15 PWKK131 pKa = 10.17 LRR133 pKa = 11.84 PSAEE137 pKa = 4.58 LPFTDD142 pKa = 5.7 DD143 pKa = 3.13 PALFKK148 pKa = 10.5 RR149 pKa = 11.84 VQHH152 pKa = 5.54 AAEE155 pKa = 4.51 RR156 pKa = 11.84 KK157 pKa = 8.91 QIPDD161 pKa = 2.79 KK162 pKa = 11.15 RR163 pKa = 11.84 MSFGNLYY170 pKa = 10.35 NHH172 pKa = 6.65 IFEE175 pKa = 4.3 YY176 pKa = 10.42 LRR178 pKa = 11.84 PIIHH182 pKa = 6.55 YY183 pKa = 9.38 IKK185 pKa = 10.57 NGPFHH190 pKa = 7.03 LPFKK194 pKa = 10.02 TSWPQDD200 pKa = 3.45 DD201 pKa = 5.14 YY202 pKa = 10.25 LTPMRR207 pKa = 11.84 SHH209 pKa = 6.71 ARR211 pKa = 11.84 SAIQPISDD219 pKa = 3.73 PNKK222 pKa = 7.97 VRR224 pKa = 11.84 FVYY227 pKa = 9.7 GYY229 pKa = 9.01 PKK231 pKa = 10.72 VGIFPQAMFFWPLFVYY247 pKa = 8.24 YY248 pKa = 9.9 HH249 pKa = 6.32 ITGQSPLLWGYY260 pKa = 7.75 EE261 pKa = 3.99 TMTGGWYY268 pKa = 10.39 RR269 pKa = 11.84 LNHH272 pKa = 6.05 EE273 pKa = 4.67 LRR275 pKa = 11.84 STPGISGSILEE286 pKa = 4.97 LDD288 pKa = 3.29 WQGFDD293 pKa = 2.56 IHH295 pKa = 8.38 ALFEE299 pKa = 4.3 VFDD302 pKa = 5.14 DD303 pKa = 4.17 IKK305 pKa = 11.04 NLSWSFFDD313 pKa = 5.15 CSHH316 pKa = 6.9 GYY318 pKa = 9.79 QPTKK322 pKa = 10.53 DD323 pKa = 3.89 YY324 pKa = 11.37 QGTSCDD330 pKa = 3.57 PDD332 pKa = 3.21 RR333 pKa = 11.84 LKK335 pKa = 11.14 NLWEE339 pKa = 4.6 WIWFSIKK346 pKa = 9.1 YY347 pKa = 8.22 TPIRR351 pKa = 11.84 LPDD354 pKa = 3.13 GRR356 pKa = 11.84 LFRR359 pKa = 11.84 RR360 pKa = 11.84 SWRR363 pKa = 11.84 GVPSGLFITQWLDD376 pKa = 3.24 SVYY379 pKa = 9.86 NCIMIFTILDD389 pKa = 3.24 AMGFEE394 pKa = 4.4 ITSDD398 pKa = 4.34 LIIKK402 pKa = 9.68 VLGDD406 pKa = 3.79 DD407 pKa = 3.33 SLTRR411 pKa = 11.84 LRR413 pKa = 11.84 LLIPYY418 pKa = 7.94 DD419 pKa = 3.17 QHH421 pKa = 5.73 EE422 pKa = 4.5 AFTIRR427 pKa = 11.84 FKK429 pKa = 11.15 HH430 pKa = 5.21 LAQYY434 pKa = 10.95 YY435 pKa = 9.21 FDD437 pKa = 5.39 AVLSDD442 pKa = 3.39 KK443 pKa = 10.66 KK444 pKa = 11.22 SRR446 pKa = 11.84 IHH448 pKa = 5.58 NTLNGVKK455 pKa = 10.42 CLGYY459 pKa = 10.39 RR460 pKa = 11.84 NNNGLPVRR468 pKa = 11.84 DD469 pKa = 3.92 RR470 pKa = 11.84 EE471 pKa = 4.41 SLLTALLYY479 pKa = 10.31 PKK481 pKa = 10.28 ARR483 pKa = 11.84 RR484 pKa = 11.84 PTFEE488 pKa = 3.85 QLKK491 pKa = 9.86 ARR493 pKa = 11.84 CIGIAYY499 pKa = 9.73 ASAGNDD505 pKa = 3.21 PTTLEE510 pKa = 4.08 VCKK513 pKa = 10.53 DD514 pKa = 3.26 VYY516 pKa = 11.23 DD517 pKa = 3.95 YY518 pKa = 11.88 LDD520 pKa = 3.63 AKK522 pKa = 10.2 GIKK525 pKa = 9.72 ADD527 pKa = 3.87 PAGLADD533 pKa = 5.77 LFDD536 pKa = 5.73 PNLGLDD542 pKa = 3.11 IDD544 pKa = 3.79 ITFFPNILTIQRR556 pKa = 11.84 SLLILRR562 pKa = 11.84 DD563 pKa = 3.52 SRR565 pKa = 11.84 EE566 pKa = 3.81 QAHH569 pKa = 6.4 RR570 pKa = 11.84 MWPTSHH576 pKa = 7.23 FLSPIPGLVDD586 pKa = 3.12 II587 pKa = 5.55
Molecular weight: 68.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.801
IPC2_protein 8.024
IPC_protein 7.907
Toseland 7.995
ProMoST 8.404
Dawson 8.653
Bjellqvist 8.77
Wikipedia 8.697
Rodwell 8.697
Grimsley 8.083
Solomon 8.741
Lehninger 8.726
Nozaki 8.814
DTASelect 8.609
Thurlkill 8.668
EMBOSS 8.814
Sillero 8.902
Patrickios 4.266
IPC_peptide 8.726
IPC2_peptide 7.614
IPC2.peptide.svr19 7.754
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1067
480
587
533.5
60.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.591 ± 1.53
1.312 ± 0.093
5.998 ± 1.163
3.374 ± 0.239
6.186 ± 0.224
4.967 ± 0.244
2.624 ± 0.741
6.373 ± 0.74
3.561 ± 1.468
8.903 ± 0.759
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.718 ± 0.523
3.561 ± 0.385
7.591 ± 0.339
4.03 ± 0.352
5.998 ± 0.237
7.404 ± 0.855
6.842 ± 1.345
5.155 ± 0.961
1.781 ± 0.337
4.03 ± 0.707
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here