Syncephalis pseudoplumigaleata

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Zoopagomycota; Zoopagomycotina; Zoopagomycetes; Zoopagales; Piptocephalidaceae; Syncephalis

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5997 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P9YT38|A0A4P9YT38_9FUNG Uncharacterized protein OS=Syncephalis pseudoplumigaleata OX=1712513 GN=SYNPS1DRAFT_24887 PE=4 SV=1
MM1 pKa = 7.51SGQLEE6 pKa = 4.42MPDD9 pKa = 3.46AASATQLATAEE20 pKa = 4.44ILNHH24 pKa = 6.42PASSQNDD31 pKa = 3.18PMIFANNNNNSNNSGDD47 pKa = 4.06SNHH50 pKa = 6.64TGAASGGIQPQHH62 pKa = 6.66LPLSPPDD69 pKa = 3.77EE70 pKa = 4.27QKK72 pKa = 11.2LEE74 pKa = 4.21CTTLDD79 pKa = 3.49ASDD82 pKa = 4.14VSWISSKK89 pKa = 11.29SMPATPYY96 pKa = 10.95ASDD99 pKa = 3.5AVTSASMFSSNVLALQQQNEE119 pKa = 4.5SASDD123 pKa = 3.49ASMSAADD130 pKa = 4.19DD131 pKa = 4.02AACEE135 pKa = 4.11GMPSCVQRR143 pKa = 11.84LLRR146 pKa = 11.84LDD148 pKa = 3.83PDD150 pKa = 3.43VWEE153 pKa = 5.14HH154 pKa = 6.43GSIDD158 pKa = 3.83SDD160 pKa = 4.22IVCCYY165 pKa = 9.67HH166 pKa = 7.57DD167 pKa = 3.77VVLRR171 pKa = 11.84VDD173 pKa = 4.15DD174 pKa = 5.22LRR176 pKa = 11.84LLSPGQWLNDD186 pKa = 2.96TWIEE190 pKa = 3.98FFYY193 pKa = 10.85EE194 pKa = 3.93

Molecular weight: 20.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1J1N7|A0A4V1J1N7_9FUNG ArAE_2 domain-containing protein OS=Syncephalis pseudoplumigaleata OX=1712513 GN=SYNPS1DRAFT_28574 PE=4 SV=1
MM1 pKa = 6.46TTSALTWLLLKK12 pKa = 10.24KK13 pKa = 10.18QSRR16 pKa = 11.84FVVKK20 pKa = 10.47RR21 pKa = 11.84AGAEE25 pKa = 4.15FNTEE29 pKa = 4.03PGNLTNKK36 pKa = 8.36NTFTSSSLVNKK47 pKa = 10.25SVVLAAGPKK56 pKa = 9.45GKK58 pKa = 9.98GVKK61 pKa = 8.44LTQPSGRR68 pKa = 11.84VVKK71 pKa = 9.1LTRR74 pKa = 11.84GAARR78 pKa = 11.84AARR81 pKa = 11.84TVSGAVRR88 pKa = 11.84PQQRR92 pKa = 11.84RR93 pKa = 11.84VALARR98 pKa = 11.84MSAILRR104 pKa = 11.84AQRR107 pKa = 11.84PVKK110 pKa = 9.79ATTFKK115 pKa = 10.29RR116 pKa = 11.84TGIRR120 pKa = 11.84ATRR123 pKa = 11.84KK124 pKa = 9.47

Molecular weight: 13.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5997

0

5997

2091956

49

3138

348.8

38.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.446 ± 0.04

1.482 ± 0.014

5.707 ± 0.025

5.796 ± 0.038

3.281 ± 0.024

6.392 ± 0.03

3.032 ± 0.018

4.665 ± 0.027

3.933 ± 0.028

9.275 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.69 ± 0.014

3.159 ± 0.018

5.343 ± 0.034

4.174 ± 0.021

6.899 ± 0.031

7.192 ± 0.041

5.923 ± 0.024

6.388 ± 0.027

1.292 ± 0.011

2.932 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski