Candidatus Mcinerneyibacterium aminivorans
Average proteome isoelectric point is 7.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2016 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5D0MKI0|A0A5D0MKI0_9BACT TIGR00282 family metallophosphoesterase OS=Candidatus Mcinerneyibacterium aminivorans OX=2703815 GN=FXF47_00975 PE=4 SV=1
MM1 pKa = 7.73 AEE3 pKa = 4.86 CPLLDD8 pKa = 3.05 QCKK11 pKa = 9.79 FYY13 pKa = 11.3 EE14 pKa = 4.54 KK15 pKa = 10.63 YY16 pKa = 10.81 QNIDD20 pKa = 3.47 DD21 pKa = 4.06 KK22 pKa = 11.28 TLLEE26 pKa = 4.28 GFVNRR31 pKa = 11.84 FCNGNFEE38 pKa = 4.2 QCVVKK43 pKa = 10.78 KK44 pKa = 9.46 ITEE47 pKa = 4.18 DD48 pKa = 3.18 HH49 pKa = 6.39 NMDD52 pKa = 3.88 YY53 pKa = 11.21 VPDD56 pKa = 3.78 NMLPDD61 pKa = 4.19 GMPEE65 pKa = 4.17 PGTDD69 pKa = 3.34 DD70 pKa = 4.76 SEE72 pKa = 4.19 WSEE75 pKa = 5.53 DD76 pKa = 3.45 IIDD79 pKa = 3.69 YY80 pKa = 8.55 MM81 pKa = 5.14
Molecular weight: 9.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.897
IPC_protein 3.846
Toseland 3.643
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.783
Rodwell 3.681
Grimsley 3.554
Solomon 3.821
Lehninger 3.783
Nozaki 3.961
DTASelect 4.177
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.973
Patrickios 0.896
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.856
Protein with the highest isoelectric point:
>tr|A0A5D0MGJ7|A0A5D0MGJ7_9BACT Uncharacterized protein OS=Candidatus Mcinerneyibacterium aminivorans OX=2703815 GN=FXF47_08475 PE=4 SV=1
MM1 pKa = 7.54 SSVKK5 pKa = 10.23 KK6 pKa = 10.28 KK7 pKa = 10.23 RR8 pKa = 11.84 KK9 pKa = 9.79 KK10 pKa = 10.52 KK11 pKa = 8.69 MNKK14 pKa = 8.81 HH15 pKa = 4.84 KK16 pKa = 10.35 RR17 pKa = 11.84 KK18 pKa = 9.58 KK19 pKa = 8.92 RR20 pKa = 11.84 SRR22 pKa = 11.84 KK23 pKa = 8.93 NRR25 pKa = 11.84 HH26 pKa = 5.12 KK27 pKa = 10.59 KK28 pKa = 9.46 KK29 pKa = 10.48 KK30 pKa = 9.44 RR31 pKa = 3.32
Molecular weight: 4.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.445
IPC2_protein 11.052
IPC_protein 12.544
Toseland 12.735
ProMoST 13.217
Dawson 12.749
Bjellqvist 12.72
Wikipedia 13.203
Rodwell 12.808
Grimsley 12.778
Solomon 13.217
Lehninger 13.115
Nozaki 12.735
DTASelect 12.72
Thurlkill 12.735
EMBOSS 13.232
Sillero 12.735
Patrickios 12.515
IPC_peptide 13.217
IPC2_peptide 12.193
IPC2.peptide.svr19 8.979
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2016
0
2016
637312
16
2339
316.1
36.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.445 ± 0.057
0.723 ± 0.018
5.612 ± 0.045
7.488 ± 0.072
5.243 ± 0.054
5.628 ± 0.06
1.425 ± 0.019
10.093 ± 0.056
11.282 ± 0.085
9.049 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.273 ± 0.026
7.031 ± 0.069
2.72 ± 0.03
2.23 ± 0.022
3.627 ± 0.031
5.89 ± 0.044
4.058 ± 0.03
5.518 ± 0.052
0.745 ± 0.016
4.921 ± 0.059
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here