Methanomassiliicoccales archaeon RumEn M1

Taxonomy: cellular organisms; Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Methanomassiliicoccales; unclassified Methanomassiliicoccales

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1877 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q4BC58|A0A0Q4BC58_9ARCH B12-binding domain-containing protein OS=Methanomassiliicoccales archaeon RumEn M1 OX=1713724 GN=AOA80_07865 PE=4 SV=1
MM1 pKa = 7.18SAEE4 pKa = 4.38TGDD7 pKa = 3.68PWTDD11 pKa = 2.8IPEE14 pKa = 4.09NEE16 pKa = 4.18IIDD19 pKa = 3.91WGEE22 pKa = 3.39MWGTVIQFQYY32 pKa = 11.1VYY34 pKa = 10.39DD35 pKa = 4.09PEE37 pKa = 4.52VGNQAQTIEE46 pKa = 4.04WDD48 pKa = 3.79FGDD51 pKa = 4.26GSPRR55 pKa = 11.84STEE58 pKa = 3.46WNPRR62 pKa = 11.84HH63 pKa = 5.82TYY65 pKa = 9.97EE66 pKa = 4.52EE67 pKa = 4.6YY68 pKa = 9.05GTYY71 pKa = 9.99IVVQHH76 pKa = 5.19VTNTYY81 pKa = 10.6EE82 pKa = 4.63GFSEE86 pKa = 3.89DD87 pKa = 2.43WGYY90 pKa = 11.59YY91 pKa = 10.14RR92 pKa = 11.84MTIMGKK98 pKa = 9.13PYY100 pKa = 10.43VEE102 pKa = 4.13IVAPEE107 pKa = 4.4GAPQMEE113 pKa = 4.52KK114 pKa = 10.79VYY116 pKa = 9.26ATKK119 pKa = 9.36GTAPEE124 pKa = 4.26QPDD127 pKa = 3.42DD128 pKa = 5.04PIWKK132 pKa = 8.36GHH134 pKa = 5.53EE135 pKa = 3.85FLGYY139 pKa = 9.93YY140 pKa = 10.35ADD142 pKa = 5.0AEE144 pKa = 4.36FTVPFDD150 pKa = 3.08WTIPLDD156 pKa = 3.94RR157 pKa = 11.84AVTAYY162 pKa = 10.53AQFQNASVIPDD173 pKa = 4.5PDD175 pKa = 5.01DD176 pKa = 4.03EE177 pKa = 6.18DD178 pKa = 6.35PIDD181 pKa = 5.56DD182 pKa = 4.6EE183 pKa = 5.43DD184 pKa = 5.43PNGTDD189 pKa = 3.14SKK191 pKa = 11.9DD192 pKa = 3.48NITAGIGEE200 pKa = 4.28FFEE203 pKa = 4.83QYY205 pKa = 10.68GALTLGIAGGIVIAVFAIGVRR226 pKa = 11.84HH227 pKa = 5.61PVVLIVGIALLVIAALLKK245 pKa = 10.8FGVII249 pKa = 4.11

Molecular weight: 27.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q4BCL3|A0A0Q4BCL3_9ARCH Histidinol-phosphate aminotransferase OS=Methanomassiliicoccales archaeon RumEn M1 OX=1713724 GN=hisC PE=3 SV=1
MM1 pKa = 7.19KK2 pKa = 9.96HH3 pKa = 6.14YY4 pKa = 10.65EE5 pKa = 3.66IDD7 pKa = 3.6KK8 pKa = 9.34YY9 pKa = 11.28AHH11 pKa = 6.37SSSLFQFDD19 pKa = 3.86PRR21 pKa = 11.84VKK23 pKa = 9.79LACAISLIVVTAFLRR38 pKa = 11.84SMEE41 pKa = 3.82AVMVVLLFTALLVLFSRR58 pKa = 11.84VPLGHH63 pKa = 6.9LWKK66 pKa = 10.48NLALAVPFIVVPSLALLFTSGPYY89 pKa = 9.2NAAVLAMRR97 pKa = 11.84ILASVLALTAVISTTPLFDD116 pKa = 4.73LLRR119 pKa = 11.84TLRR122 pKa = 11.84WFRR125 pKa = 11.84MPKK128 pKa = 9.76LLSSIMMFAYY138 pKa = 10.16RR139 pKa = 11.84FIFVLIDD146 pKa = 3.02EE147 pKa = 4.62MDD149 pKa = 3.65RR150 pKa = 11.84MKK152 pKa = 9.9MARR155 pKa = 11.84MARR158 pKa = 11.84GYY160 pKa = 8.73TGRR163 pKa = 11.84GNILSRR169 pKa = 11.84DD170 pKa = 3.61VFRR173 pKa = 11.84TLSFTAGMIFVRR185 pKa = 11.84SNSRR189 pKa = 11.84ATRR192 pKa = 11.84IYY194 pKa = 10.81DD195 pKa = 3.4ALLARR200 pKa = 11.84GYY202 pKa = 10.73SGDD205 pKa = 3.25VHH207 pKa = 6.33TLDD210 pKa = 3.14RR211 pKa = 11.84MKK213 pKa = 10.82VRR215 pKa = 11.84GRR217 pKa = 11.84DD218 pKa = 3.55VPSAQCSSASEE229 pKa = 4.09RR230 pKa = 3.77

Molecular weight: 25.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1877

0

1877

479373

44

2088

255.4

28.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.212 ± 0.069

1.23 ± 0.028

5.977 ± 0.049

7.174 ± 0.064

3.447 ± 0.038

8.189 ± 0.06

1.906 ± 0.025

5.819 ± 0.051

4.073 ± 0.054

9.692 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.371 ± 0.03

2.755 ± 0.038

4.5 ± 0.034

2.522 ± 0.026

7.037 ± 0.077

6.099 ± 0.051

4.958 ± 0.047

8.214 ± 0.05

1.069 ± 0.021

2.757 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski