Amino acid dipepetide frequency for Methanomassiliicoccales archaeon RumEn M1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.581AlaAla: 9.581 ± 0.185
1.072AlaCys: 1.072 ± 0.044
4.99AlaAsp: 4.99 ± 0.105
6.41AlaGlu: 6.41 ± 0.135
3.386AlaPhe: 3.386 ± 0.093
7.078AlaGly: 7.078 ± 0.116
1.583AlaHis: 1.583 ± 0.061
5.787AlaIle: 5.787 ± 0.12
3.557AlaLys: 3.557 ± 0.099
9.777AlaLeu: 9.777 ± 0.182
3.444AlaMet: 3.444 ± 0.096
2.201AlaAsn: 2.201 ± 0.073
3.471AlaPro: 3.471 ± 0.096
2.328AlaGln: 2.328 ± 0.068
6.194AlaArg: 6.194 ± 0.139
6.0AlaSer: 6.0 ± 0.113
4.118AlaThr: 4.118 ± 0.095
7.798AlaVal: 7.798 ± 0.138
0.912AlaTrp: 0.912 ± 0.045
2.43AlaTyr: 2.43 ± 0.069
0.0AlaXaa: 0.0 ± 0.0
Cys
0.974CysAla: 0.974 ± 0.051
0.202CysCys: 0.202 ± 0.024
0.797CysAsp: 0.797 ± 0.036
0.657CysGlu: 0.657 ± 0.037
0.325CysPhe: 0.325 ± 0.026
1.435CysGly: 1.435 ± 0.062
0.261CysHis: 0.261 ± 0.022
0.58CysIle: 0.58 ± 0.037
0.39CysLys: 0.39 ± 0.027
0.962CysLeu: 0.962 ± 0.048
0.338CysMet: 0.338 ± 0.024
0.325CysAsn: 0.325 ± 0.031
1.049CysPro: 1.049 ± 0.052
0.353CysGln: 0.353 ± 0.025
0.926CysArg: 0.926 ± 0.05
0.793CysSer: 0.793 ± 0.045
0.686CysThr: 0.686 ± 0.043
0.77CysVal: 0.77 ± 0.046
0.148CysTrp: 0.148 ± 0.016
0.325CysTyr: 0.325 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
5.528AspAla: 5.528 ± 0.112
0.659AspCys: 0.659 ± 0.04
3.427AspAsp: 3.427 ± 0.089
4.558AspGlu: 4.558 ± 0.108
1.794AspPhe: 1.794 ± 0.066
5.224AspGly: 5.224 ± 0.129
1.389AspHis: 1.389 ± 0.061
3.953AspIle: 3.953 ± 0.089
1.944AspLys: 1.944 ± 0.066
6.498AspLeu: 6.498 ± 0.113
2.015AspMet: 2.015 ± 0.066
1.45AspAsn: 1.45 ± 0.068
3.069AspPro: 3.069 ± 0.078
1.416AspGln: 1.416 ± 0.058
4.516AspArg: 4.516 ± 0.105
2.487AspSer: 2.487 ± 0.068
2.174AspThr: 2.174 ± 0.07
5.301AspVal: 5.301 ± 0.1
0.726AspTrp: 0.726 ± 0.036
1.646AspTyr: 1.646 ± 0.068
0.0AspXaa: 0.0 ± 0.0
Glu
7.103GluAla: 7.103 ± 0.152
0.747GluCys: 0.747 ± 0.048
3.991GluAsp: 3.991 ± 0.096
6.319GluGlu: 6.319 ± 0.175
2.007GluPhe: 2.007 ± 0.062
5.776GluGly: 5.776 ± 0.117
1.525GluHis: 1.525 ± 0.062
3.87GluIle: 3.87 ± 0.102
3.35GluLys: 3.35 ± 0.095
7.276GluLeu: 7.276 ± 0.148
2.641GluMet: 2.641 ± 0.081
1.919GluAsn: 1.919 ± 0.068
2.595GluPro: 2.595 ± 0.092
2.009GluGln: 2.009 ± 0.065
6.168GluArg: 6.168 ± 0.16
3.129GluSer: 3.129 ± 0.09
2.683GluThr: 2.683 ± 0.081
6.106GluVal: 6.106 ± 0.12
0.816GluTrp: 0.816 ± 0.041
1.706GluTyr: 1.706 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
2.827PheAla: 2.827 ± 0.078
0.419PheCys: 0.419 ± 0.031
2.249PheAsp: 2.249 ± 0.068
2.247PheGlu: 2.247 ± 0.068
1.35PhePhe: 1.35 ± 0.069
2.958PheGly: 2.958 ± 0.09
0.686PheHis: 0.686 ± 0.035
1.996PheIle: 1.996 ± 0.071
1.214PheLys: 1.214 ± 0.042
3.388PheLeu: 3.388 ± 0.098
1.018PheMet: 1.018 ± 0.051
1.083PheAsn: 1.083 ± 0.047
1.656PhePro: 1.656 ± 0.066
0.989PheGln: 0.989 ± 0.045
2.124PheArg: 2.124 ± 0.069
2.268PheSer: 2.268 ± 0.073
1.861PheThr: 1.861 ± 0.064
2.729PheVal: 2.729 ± 0.073
0.39PheTrp: 0.39 ± 0.029
1.022PheTyr: 1.022 ± 0.045
0.0PheXaa: 0.0 ± 0.0
Gly
7.105GlyAla: 7.105 ± 0.124
1.172GlyCys: 1.172 ± 0.051
4.31GlyAsp: 4.31 ± 0.108
5.278GlyGlu: 5.278 ± 0.105
2.789GlyPhe: 2.789 ± 0.088
6.469GlyGly: 6.469 ± 0.123
1.723GlyHis: 1.723 ± 0.059
5.111GlyIle: 5.111 ± 0.107
3.938GlyLys: 3.938 ± 0.092
7.725GlyLeu: 7.725 ± 0.158
3.05GlyMet: 3.05 ± 0.089
2.234GlyAsn: 2.234 ± 0.081
3.208GlyPro: 3.208 ± 0.08
2.13GlyGln: 2.13 ± 0.065
6.292GlyArg: 6.292 ± 0.156
5.013GlySer: 5.013 ± 0.112
4.758GlyThr: 4.758 ± 0.096
6.227GlyVal: 6.227 ± 0.135
1.047GlyTrp: 1.047 ± 0.051
2.316GlyTyr: 2.316 ± 0.078
0.0GlyXaa: 0.0 ± 0.0
His
1.523HisAla: 1.523 ± 0.054
0.292HisCys: 0.292 ± 0.026
1.108HisAsp: 1.108 ± 0.05
1.197HisGlu: 1.197 ± 0.056
0.661HisPhe: 0.661 ± 0.034
1.592HisGly: 1.592 ± 0.067
0.58HisHis: 0.58 ± 0.039
1.087HisIle: 1.087 ± 0.051
0.519HisLys: 0.519 ± 0.034
2.078HisLeu: 2.078 ± 0.066
0.653HisMet: 0.653 ± 0.04
0.459HisAsn: 0.459 ± 0.031
1.166HisPro: 1.166 ± 0.055
0.511HisGln: 0.511 ± 0.039
1.446HisArg: 1.446 ± 0.065
1.037HisSer: 1.037 ± 0.043
0.805HisThr: 0.805 ± 0.043
1.498HisVal: 1.498 ± 0.053
0.207HisTrp: 0.207 ± 0.021
0.64HisTyr: 0.64 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
5.524IleAla: 5.524 ± 0.105
0.688IleCys: 0.688 ± 0.041
4.041IleAsp: 4.041 ± 0.1
4.208IleGlu: 4.208 ± 0.121
1.713IlePhe: 1.713 ± 0.067
5.148IleGly: 5.148 ± 0.112
0.887IleHis: 0.887 ± 0.044
3.761IleIle: 3.761 ± 0.101
2.274IleLys: 2.274 ± 0.076
4.881IleLeu: 4.881 ± 0.143
1.825IleMet: 1.825 ± 0.058
1.61IleAsn: 1.61 ± 0.063
2.745IlePro: 2.745 ± 0.084
1.197IleGln: 1.197 ± 0.051
3.348IleArg: 3.348 ± 0.094
3.911IleSer: 3.911 ± 0.101
3.16IleThr: 3.16 ± 0.078
5.19IleVal: 5.19 ± 0.1
0.534IleTrp: 0.534 ± 0.038
1.548IleTyr: 1.548 ± 0.063
0.0IleXaa: 0.0 ± 0.0
Lys
4.183LysAla: 4.183 ± 0.118
0.478LysCys: 0.478 ± 0.038
2.608LysAsp: 2.608 ± 0.08
3.55LysGlu: 3.55 ± 0.099
1.081LysPhe: 1.081 ± 0.049
3.759LysGly: 3.759 ± 0.089
0.732LysHis: 0.732 ± 0.037
2.128LysIle: 2.128 ± 0.084
2.272LysLys: 2.272 ± 0.095
3.238LysLeu: 3.238 ± 0.094
1.368LysMet: 1.368 ± 0.053
1.212LysAsn: 1.212 ± 0.058
1.27LysPro: 1.27 ± 0.054
0.926LysGln: 0.926 ± 0.042
2.929LysArg: 2.929 ± 0.078
1.742LysSer: 1.742 ± 0.056
1.738LysThr: 1.738 ± 0.062
4.053LysVal: 4.053 ± 0.089
0.388LysTrp: 0.388 ± 0.029
1.072LysTyr: 1.072 ± 0.053
0.0LysXaa: 0.0 ± 0.0
Leu
9.333LeuAla: 9.333 ± 0.178
1.031LeuCys: 1.031 ± 0.048
6.396LeuAsp: 6.396 ± 0.133
6.857LeuGlu: 6.857 ± 0.145
3.598LeuPhe: 3.598 ± 0.105
7.566LeuGly: 7.566 ± 0.139
1.625LeuHis: 1.625 ± 0.074
5.173LeuIle: 5.173 ± 0.133
3.974LeuLys: 3.974 ± 0.095
9.571LeuLeu: 9.571 ± 0.232
3.05LeuMet: 3.05 ± 0.093
2.754LeuAsn: 2.754 ± 0.078
4.548LeuPro: 4.548 ± 0.109
2.353LeuGln: 2.353 ± 0.069
6.821LeuArg: 6.821 ± 0.146
6.675LeuSer: 6.675 ± 0.123
4.825LeuThr: 4.825 ± 0.105
7.597LeuVal: 7.597 ± 0.145
0.818LeuTrp: 0.818 ± 0.043
2.351LeuTyr: 2.351 ± 0.071
0.0LeuXaa: 0.0 ± 0.0
Met
3.356MetAla: 3.356 ± 0.083
0.332MetCys: 0.332 ± 0.026
2.119MetAsp: 2.119 ± 0.07
2.568MetGlu: 2.568 ± 0.079
1.129MetPhe: 1.129 ± 0.053
2.737MetGly: 2.737 ± 0.072
0.557MetHis: 0.557 ± 0.033
1.583MetIle: 1.583 ± 0.059
1.51MetLys: 1.51 ± 0.056
3.129MetLeu: 3.129 ± 0.088
1.02MetMet: 1.02 ± 0.05
1.139MetAsn: 1.139 ± 0.048
1.435MetPro: 1.435 ± 0.054
0.701MetGln: 0.701 ± 0.045
2.259MetArg: 2.259 ± 0.069
2.18MetSer: 2.18 ± 0.067
1.886MetThr: 1.886 ± 0.06
3.046MetVal: 3.046 ± 0.079
0.261MetTrp: 0.261 ± 0.022
0.761MetTyr: 0.761 ± 0.043
0.0MetXaa: 0.0 ± 0.0
Asn
2.42AsnAla: 2.42 ± 0.071
0.398AsnCys: 0.398 ± 0.034
1.598AsnAsp: 1.598 ± 0.06
1.976AsnGlu: 1.976 ± 0.058
0.893AsnPhe: 0.893 ± 0.042
2.639AsnGly: 2.639 ± 0.083
0.453AsnHis: 0.453 ± 0.032
2.082AsnIle: 2.082 ± 0.079
1.018AsnLys: 1.018 ± 0.05
2.487AsnLeu: 2.487 ± 0.063
0.876AsnMet: 0.876 ± 0.041
0.834AsnAsn: 0.834 ± 0.046
1.352AsnPro: 1.352 ± 0.068
0.615AsnGln: 0.615 ± 0.035
1.64AsnArg: 1.64 ± 0.057
1.25AsnSer: 1.25 ± 0.051
1.285AsnThr: 1.285 ± 0.052
2.628AsnVal: 2.628 ± 0.084
0.309AsnTrp: 0.309 ± 0.025
0.797AsnTyr: 0.797 ± 0.046
0.0AsnXaa: 0.0 ± 0.0
Pro
3.676ProAla: 3.676 ± 0.083
0.496ProCys: 0.496 ± 0.035
2.864ProAsp: 2.864 ± 0.086
3.778ProGlu: 3.778 ± 0.096
1.961ProPhe: 1.961 ± 0.073
3.715ProGly: 3.715 ± 0.082
0.974ProHis: 0.974 ± 0.047
2.13ProIle: 2.13 ± 0.072
1.596ProLys: 1.596 ± 0.057
4.135ProLeu: 4.135 ± 0.105
1.325ProMet: 1.325 ± 0.062
1.179ProAsn: 1.179 ± 0.049
2.023ProPro: 2.023 ± 0.079
1.225ProGln: 1.225 ± 0.053
2.985ProArg: 2.985 ± 0.083
3.457ProSer: 3.457 ± 0.093
2.207ProThr: 2.207 ± 0.072
3.505ProVal: 3.505 ± 0.096
0.48ProTrp: 0.48 ± 0.032
1.291ProTyr: 1.291 ± 0.057
0.0ProXaa: 0.0 ± 0.0
Gln
2.631GlnAla: 2.631 ± 0.08
0.317GlnCys: 0.317 ± 0.03
1.389GlnAsp: 1.389 ± 0.056
2.007GlnGlu: 2.007 ± 0.067
0.82GlnPhe: 0.82 ± 0.038
2.053GlnGly: 2.053 ± 0.065
0.547GlnHis: 0.547 ± 0.041
1.306GlnIle: 1.306 ± 0.048
1.026GlnLys: 1.026 ± 0.047
2.318GlnLeu: 2.318 ± 0.085
0.893GlnMet: 0.893 ± 0.042
0.73GlnAsn: 0.73 ± 0.043
0.891GlnPro: 0.891 ± 0.037
0.816GlnGln: 0.816 ± 0.052
1.998GlnArg: 1.998 ± 0.064
1.272GlnSer: 1.272 ± 0.052
1.197GlnThr: 1.197 ± 0.053
2.011GlnVal: 2.011 ± 0.059
0.263GlnTrp: 0.263 ± 0.022
0.741GlnTyr: 0.741 ± 0.039
0.0GlnXaa: 0.0 ± 0.0
Arg
5.828ArgAla: 5.828 ± 0.145
1.022ArgCys: 1.022 ± 0.048
4.047ArgAsp: 4.047 ± 0.096
5.37ArgGlu: 5.37 ± 0.124
2.52ArgPhe: 2.52 ± 0.085
4.796ArgGly: 4.796 ± 0.117
1.298ArgHis: 1.298 ± 0.052
3.913ArgIle: 3.913 ± 0.094
3.221ArgLys: 3.221 ± 0.095
6.571ArgLeu: 6.571 ± 0.141
2.747ArgMet: 2.747 ± 0.074
1.932ArgAsn: 1.932 ± 0.06
3.369ArgPro: 3.369 ± 0.085
1.988ArgGln: 1.988 ± 0.064
5.593ArgArg: 5.593 ± 0.174
4.879ArgSer: 4.879 ± 0.118
3.872ArgThr: 3.872 ± 0.095
4.289ArgVal: 4.289 ± 0.105
0.864ArgTrp: 0.864 ± 0.048
2.253ArgTyr: 2.253 ± 0.065
0.0ArgXaa: 0.0 ± 0.0
Ser
5.397SerAla: 5.397 ± 0.106
0.822SerCys: 0.822 ± 0.044
3.192SerAsp: 3.192 ± 0.078
3.826SerGlu: 3.826 ± 0.091
2.401SerPhe: 2.401 ± 0.075
5.163SerGly: 5.163 ± 0.114
0.987SerHis: 0.987 ± 0.05
3.847SerIle: 3.847 ± 0.088
2.491SerLys: 2.491 ± 0.082
5.997SerLeu: 5.997 ± 0.134
2.167SerMet: 2.167 ± 0.075
1.558SerAsn: 1.558 ± 0.068
2.839SerPro: 2.839 ± 0.087
1.427SerGln: 1.427 ± 0.061
3.905SerArg: 3.905 ± 0.098
4.316SerSer: 4.316 ± 0.12
3.302SerThr: 3.302 ± 0.087
4.767SerVal: 4.767 ± 0.107
0.768SerTrp: 0.768 ± 0.037
1.815SerTyr: 1.815 ± 0.066
0.0SerXaa: 0.0 ± 0.0
Thr
4.773ThrAla: 4.773 ± 0.112
0.576ThrCys: 0.576 ± 0.038
2.653ThrAsp: 2.653 ± 0.073
2.668ThrGlu: 2.668 ± 0.08
1.842ThrPhe: 1.842 ± 0.063
4.493ThrGly: 4.493 ± 0.107
0.843ThrHis: 0.843 ± 0.042
3.265ThrIle: 3.265 ± 0.088
1.848ThrLys: 1.848 ± 0.069
4.579ThrLeu: 4.579 ± 0.096
1.673ThrMet: 1.673 ± 0.061
1.398ThrAsn: 1.398 ± 0.061
2.443ThrPro: 2.443 ± 0.086
0.962ThrGln: 0.962 ± 0.046
2.539ThrArg: 2.539 ± 0.082
3.302ThrSer: 3.302 ± 0.1
2.866ThrThr: 2.866 ± 0.089
4.838ThrVal: 4.838 ± 0.114
0.513ThrTrp: 0.513 ± 0.035
1.504ThrTyr: 1.504 ± 0.061
0.0ThrXaa: 0.0 ± 0.0
Val
7.209ValAla: 7.209 ± 0.143
1.022ValCys: 1.022 ± 0.048
5.353ValAsp: 5.353 ± 0.102
5.766ValGlu: 5.766 ± 0.111
2.795ValPhe: 2.795 ± 0.073
6.056ValGly: 6.056 ± 0.129
1.627ValHis: 1.627 ± 0.06
4.614ValIle: 4.614 ± 0.087
3.215ValLys: 3.215 ± 0.09
8.449ValLeu: 8.449 ± 0.149
2.485ValMet: 2.485 ± 0.081
2.322ValAsn: 2.322 ± 0.062
4.174ValPro: 4.174 ± 0.096
2.19ValGln: 2.19 ± 0.074
5.908ValArg: 5.908 ± 0.126
4.867ValSer: 4.867 ± 0.103
4.322ValThr: 4.322 ± 0.109
6.963ValVal: 6.963 ± 0.136
0.734ValTrp: 0.734 ± 0.044
2.067ValTyr: 2.067 ± 0.071
0.0ValXaa: 0.0 ± 0.0
Trp
0.912TrpAla: 0.912 ± 0.043
0.138TrpCys: 0.138 ± 0.017
0.684TrpAsp: 0.684 ± 0.041
0.695TrpGlu: 0.695 ± 0.039
0.369TrpPhe: 0.369 ± 0.029
0.747TrpGly: 0.747 ± 0.04
0.165TrpHis: 0.165 ± 0.018
0.574TrpIle: 0.574 ± 0.034
0.53TrpLys: 0.53 ± 0.035
1.028TrpLeu: 1.028 ± 0.053
0.378TrpMet: 0.378 ± 0.028
0.392TrpAsn: 0.392 ± 0.027
0.411TrpPro: 0.411 ± 0.032
0.305TrpGln: 0.305 ± 0.025
0.734TrpArg: 0.734 ± 0.039
0.774TrpSer: 0.774 ± 0.038
0.651TrpThr: 0.651 ± 0.037
0.711TrpVal: 0.711 ± 0.037
0.173TrpTrp: 0.173 ± 0.022
0.323TrpTyr: 0.323 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.236TyrAla: 2.236 ± 0.065
0.413TyrCys: 0.413 ± 0.03
1.959TyrAsp: 1.959 ± 0.065
1.802TyrGlu: 1.802 ± 0.061
1.039TyrPhe: 1.039 ± 0.048
2.491TyrGly: 2.491 ± 0.079
0.617TyrHis: 0.617 ± 0.033
1.435TyrIle: 1.435 ± 0.057
0.839TyrLys: 0.839 ± 0.048
2.831TyrLeu: 2.831 ± 0.08
0.741TyrMet: 0.741 ± 0.042
0.818TyrAsn: 0.818 ± 0.044
1.285TyrPro: 1.285 ± 0.061
0.784TyrGln: 0.784 ± 0.045
2.046TyrArg: 2.046 ± 0.06
1.635TyrSer: 1.635 ± 0.059
1.181TyrThr: 1.181 ± 0.046
2.113TyrVal: 2.113 ± 0.069
0.344TyrTrp: 0.344 ± 0.027
0.955TyrTyr: 0.955 ± 0.055
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1877 proteins (479373 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski