Fistulina hepatica ATCC 64428

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Fistulinaceae; Fistulina; Fistulina hepatica

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9322 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D7A8A7|A0A0D7A8A7_9AGAR Uncharacterized protein OS=Fistulina hepatica ATCC 64428 OX=1128425 GN=FISHEDRAFT_59972 PE=4 SV=1
II1 pKa = 7.43SSGWYY6 pKa = 8.91PGWSYY11 pKa = 11.38TEE13 pKa = 4.64SPASDD18 pKa = 4.2LSWSKK23 pKa = 8.46YY24 pKa = 5.83THH26 pKa = 5.98MAFAFATTTSDD37 pKa = 3.58PSVLSLDD44 pKa = 3.89DD45 pKa = 3.95SGGGDD50 pKa = 3.56ALTSFVSAAKK60 pKa = 9.76EE61 pKa = 4.15NNVSALLSVGGWSGSVYY78 pKa = 10.47FSPAMEE84 pKa = 4.28KK85 pKa = 10.6SNRR88 pKa = 11.84ATFVQTIVGLADD100 pKa = 4.12KK101 pKa = 10.88YY102 pKa = 11.63DD103 pKa = 4.21LDD105 pKa = 4.63GVDD108 pKa = 6.2FDD110 pKa = 4.27WEE112 pKa = 4.51YY113 pKa = 10.82PNSDD117 pKa = 2.74GAGNQKK123 pKa = 10.25SSSDD127 pKa = 3.25ARR129 pKa = 11.84NFLAFLKK136 pKa = 10.4DD137 pKa = 3.72LRR139 pKa = 11.84ATDD142 pKa = 3.66TGSKK146 pKa = 10.21LILSASVAITPFNGADD162 pKa = 3.98GEE164 pKa = 4.38PMSDD168 pKa = 2.86VSDD171 pKa = 3.87FADD174 pKa = 3.23VLDD177 pKa = 4.16YY178 pKa = 10.62VQIMAYY184 pKa = 10.28DD185 pKa = 3.66IFTTSSATVGPNAPFQDD202 pKa = 3.41EE203 pKa = 4.57CAASDD208 pKa = 3.85DD209 pKa = 4.0QVGSVVSASDD219 pKa = 3.1VWVDD223 pKa = 3.25AGFPASQILLGVPSYY238 pKa = 11.32GYY240 pKa = 10.37AFVVSEE246 pKa = 4.09EE247 pKa = 4.07DD248 pKa = 3.54AYY250 pKa = 11.67NADD253 pKa = 3.9GLLAEE258 pKa = 4.86YY259 pKa = 9.81PSFDD263 pKa = 3.93ASATPGGASDD273 pKa = 4.63DD274 pKa = 3.77DD275 pKa = 4.52GYY277 pKa = 11.78YY278 pKa = 10.78DD279 pKa = 3.96FSGMIKK285 pKa = 10.48DD286 pKa = 4.05ALLDD290 pKa = 3.61EE291 pKa = 5.36AGEE294 pKa = 4.3VMSNVSYY301 pKa = 11.06RR302 pKa = 11.84FDD304 pKa = 3.72NCSHH308 pKa = 6.61TPYY311 pKa = 10.19IYY313 pKa = 10.22DD314 pKa = 3.48SKK316 pKa = 9.62TSTMVSFDD324 pKa = 3.73DD325 pKa = 4.05AKK327 pKa = 11.12SFAVKK332 pKa = 10.38GSYY335 pKa = 9.86IVDD338 pKa = 3.37KK339 pKa = 10.52GLKK342 pKa = 9.12GFAMWEE348 pKa = 4.31TVSDD352 pKa = 4.15YY353 pKa = 11.67NDD355 pKa = 3.38ILLDD359 pKa = 3.91SIRR362 pKa = 11.84ASLGG366 pKa = 3.24

Molecular weight: 38.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D7AAF6|A0A0D7AAF6_9AGAR Uncharacterized protein (Fragment) OS=Fistulina hepatica ATCC 64428 OX=1128425 GN=FISHEDRAFT_21244 PE=4 SV=1
TT1 pKa = 7.29PALPHH6 pKa = 6.3SRR8 pKa = 11.84TPALPHH14 pKa = 6.23SRR16 pKa = 11.84TPALPHH22 pKa = 6.23SRR24 pKa = 11.84TPALPHH30 pKa = 6.26SRR32 pKa = 11.84TPTLPHH38 pKa = 6.41SRR40 pKa = 11.84TPALPHH46 pKa = 6.61PRR48 pKa = 11.84TPSLSHH54 pKa = 5.78SHH56 pKa = 5.26TT57 pKa = 3.69

Molecular weight: 6.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9322

0

9322

3775801

49

3562

405.0

44.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.76 ± 0.025

1.437 ± 0.009

5.867 ± 0.02

5.677 ± 0.023

3.821 ± 0.015

6.177 ± 0.022

2.669 ± 0.01

4.838 ± 0.017

4.166 ± 0.023

9.214 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.212 ± 0.011

3.328 ± 0.012

6.245 ± 0.028

3.584 ± 0.016

6.408 ± 0.024

8.535 ± 0.035

6.012 ± 0.021

6.754 ± 0.018

1.462 ± 0.009

2.835 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski